Trifluoroacylate primary and secondary amines, hydroxyl and thiol groups, and carbohydrates with the Thermo Scientific™ MBTFA Acylation Reagent. The principal byproduct N-methyltrifluoroacetamine is stable, volatile, and does not present problems in subsequent GC. MBTFA produces very volatile derivatives of carbohydrates and can be used to selectively acylate amines in the presence of hydroxyl and carboxyl groups that have been protected by silylation.
Use Thermo Scientific™ MSTFA and MSTFA + 1% TMCS Silylation Reagent for GC analyses of early eluting compounds that would otherwise be obscured in the chromatogram. MSTFA, the most volatile TMS-amide available, and its byproduct N-methyltrifluoroacetamide typically elute with the solvent front, allowing TMS derivatives of small molecules to be analyzed. Addition of Thermo Scientific™ TMCS aids derivatization of amides, secondary amines, and hindered hydroxyls not derivatized by MSTFA alone.
Prepare fluoracyl derivatives for GC/MS and for flame ionization and electron-capture detection techniques with the Thermo Scientific™ Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA). These reagents react readily with alcohols, phenols, and amines.
Thermo Scientific™ HMDS (Hexamethyldisilazane) greatly extends the practical range of GC, improving chromatographic results in the silylation of sugars and related substances.
Improve GC analysis with Thermo Scientific™ BSTFA Silylation Reagent. The highly volatile byproducts mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front to ensure excellent chromatographic separations. This silylation reagent reacts with a wide range of polar compounds and is particularly well suited for the GC analyses of some lower boiling TMS-amino acids and TMS Krebs cycle acids.
Derivatize hydroxyls compounds, particularly carbohydrates with Thermo Scientific™ Tri-Sil TP (TMSI:Pyridine) Reagent. Comprising TMSI in pyridine, this reagent silylates alcohols and phenols, organic acids, hydroxylamines, amino acids, carbohydrates, flavonoids, glycols and polyglycols, nucleotides, steroids, hydroxyl acids, barbituates, narcotics, indoles, and vitamins.
Employ a reagent-catalyst solvent mixture, for one-step derivatization with the Thermo Scientific™ Tri-Sil HTP (HDMS:TMCS:Pyridine) Reagent, Composed of HMDS, TMCS and high-purity pyridine, it rapidly produces TMS derivatives of polar compounds for GC analysis and biochemical synthesis. This versatile reagent is ideally suited for GC determinations of a range of compounds including sugars, alcohols, phenols, steroids, sterols, organic acids, and some amines.
Derivatize analytes quickly and quantitatively under mild conditions using Thermo Scientific™ BSA Silylation Reagent. This reagent reacts readily with a wide range of compounds, including alcohols, amines, carboxylic acids, phenols, steroids, biogenic amines, and alkaloids to form volatile, stable TMS derivatives for GC.
Produce stable TBDMS derivatives for GC analysis with the Thermo Scientific™ MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent. MTBSTFA derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines with typically >96% yields, and the reaction byproducts are volatile and neutral. TBDMS ethers are 104 times more stable to hydrolysis than the corresponding TMS ethers. Addition of Thermo Scientific™ TBDMCS increases silylation potential.
Use Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent for analytes that are difficult to silylate. Stronger than BSTFA alone, this catalyzed formulation is ideal for derivatizing compounds such as fatty acid amides and slightly hindered hydroxyls for GC analysis.
Use specially manufactured and packaged Thermo Scientific™ Silylation Grade Solvents to meet the exacting needs of silylation and other sensitive derivatization reactions. They are purified, dried, and packaged under nitrogen in convenient Thermo Scientific™ Hypo-Vial™ Sample Storage Vials, and are supplied with elastomer septa to enable immediate access to the sample without exposure to moisture and oxygen. Use polar solvents (acetonitrile, dimethylformamide, dimethylsulfoxide, pyridine, tetrahyrdrofuran) to facilitate reactions; nonpolar organic solvents may be used but will not accelerate the rate of reaction.
Catalyze the derivatization of difficult-to-silylate compounds using Thermo Scientific™ TMCS Silylation Reagent. It is an excellent adjunct for forming TMS ethers for GC determinations. TMCS is also useful for preparing TMS derivatives of organic acids.
For accurate, sensitive, on-column methylation and detection by GC analysis use Thermo Scientific™ MethElute™ Reagent (TMPAH). It is ideally suited for the quantitative methylation and detection of barbiturates, sedatives, xanthine bases, phenolic alkaloids, and phenytoin, giving a single quantitative peak for each substance. When the reagent is heated with drug-containing extracts, serum, or urine the drugs containing reactive amino, hydroxyl, and carboxyl functions will be methylated at the reactive sites.
Employ the strongest silylator available for carbohydrates and steroids with the Thermo Scientific™ TMSI Silylation Reagent. This silylation reagent reacts quickly with hydroxyls and carboxylic acids but not with amines, and is particularly well suited in multiderivatization schemes for compounds containing both hydroxyl and amine groups. TMSI may be used to derivatize alcohols, phenols, organic acids, hormones, glycols, nucleotides, and narcotics and is excellent for C1–C5 fatty acids in serum and urine.
Derivatize carboxyl acids, phenols, and sulfonamides for electron-capture detection (ECD) GC analysis with the Thermo Scientific™ Pentafluorobenzyl Bromide (PFBBr) Alkylation Reagent. Reaction times are fast (~20 minutes). Derivatives are highly EC-sensitive, making them useful in low-level determinations of fatty acids. PFBBr has been applied in analyzing trace organics in asphalts and as a fingerprinting technique for identifying asphalt pollutants found in surface waters.