Gc Derivatization Reagents

MethElute™ Reagent (TMPAH) Thermo Scientific™

For accurate, sensitive, on-column methylation and detection by GC analysis use Thermo Scientific™ MethElute™ Reagent (TMPAH). It is ideally suited for the quantitative methylation and detection of barbiturates, sedatives, xanthine bases, phenolic alkaloids, and phenytoin, giving a single quantitative peak for each substance. When the reagent is heated with drug-containing extracts, serum, or urine the drugs containing reactive amino, hydroxyl, and carboxyl functions will be methylated at the reactive sites.

Methylate Reagent (DMFDMA) Thermo Scientific™

For easy, effective preparation of methyl esters from fatty acids and amino acids use Thermo Scientific™ Methylate Reagent (DMFDMA). Reactions are typically complete upon dissolution (except for long-chain solid acids) and quantitative yields are obtained when reagent and sample are injected without prior mixing. No water washing, extraction, or concentration of derivatives is required and no water is formed. Methylate Reagent (DMFDMA) contains 2 mEq/mL in pyridine and is supplied in ready-to-use Thermo Scientific™ Hypo-Vial™ Sample Storage Vials.

MBTFA Acylation Reagent Thermo Scientific™

Trifluoroacylate primary and secondary amines, hydroxyl and thiol groups, and carbohydrates with the Thermo Scientific™ MBTFA Acylation Reagent. The principal byproduct N-methyltrifluoroacetamine is stable, volatile, and does not present problems in subsequent GC. MBTFA produces very volatile derivatives of carbohydrates and can be used to selectively acylate amines in the presence of hydroxyl and carboxyl groups that have been protected by silylation.

Silylation Grade Solvents Thermo Scientific™

Use specially manufactured and packaged Thermo Scientific™ Silylation Grade Solvents to meet the exacting needs of silylation and other sensitive derivatization reactions. They are purified, dried, and packaged under nitrogen in convenient Thermo Scientific™ Hypo-Vial™ Sample Storage Vials, and are supplied with elastomer septa to enable immediate access to the sample without exposure to moisture and oxygen. Use polar solvents (acetonitrile, dimethylformamide, dimethylsulfoxide, pyridine, tetrahyrdrofuran) to facilitate reactions; nonpolar organic solvents may be used but will not accelerate the rate of reaction.

BSTFA + 1% TMCS Silylation Reagent Thermo Scientific™

Use Thermo Scientific™ BSTFA + 1% TMCS Silylation Reagent for analytes that are difficult to silylate. Stronger than BSTFA alone, this catalyzed formulation is ideal for derivatizing compounds such as fatty acid amides and slightly hindered hydroxyls for GC analysis.

Methoxamine (MOX) Reagent Thermo Scientific™

Prepare oximes of steroids and ketoacids prior to silylation with the Thermo Scientific™ Methoxamine (MOX) Reagent. It prevents the formation of multiple derivatives when enols are present during silylation, thereby ensuring optimal quantitation. The reagent is a 2% solution of methoxyamine–hydrogen chloride in pyridine.

MSTFA and MSTFA + 1% TMCS Silylation Reagent Thermo Scientific™

Use Thermo Scientific™ MSTFA and MSTFA + 1% TMCS Silylation Reagent for GC analyses of early eluting compounds that would otherwise be obscured in the chromatogram. MSTFA, the most volatile TMS-amide available, and its byproduct N-methyltrifluoroacetamide typically elute with the solvent front, allowing TMS derivatives of small molecules to be analyzed. Addition of Thermo Scientific™ TMCS aids derivatization of amides, secondary amines, and hindered hydroxyls not derivatized by MSTFA alone.

BSTFA Silylation Reagent Thermo Scientific™

Improve GC analysis with Thermo Scientific™ BSTFA Silylation Reagent. The highly volatile byproducts mono(trimethylsilyl)trifluoroacetamide (BSTFA) and trifluoroacetamide elute with the solvent front to ensure excellent chromatographic separations. This silylation reagent reacts with a wide range of polar compounds and is particularly well suited for the GC analyses of some lower boiling TMS-amino acids and TMS Krebs cycle acids.

BSA Silylation Reagent Thermo Scientific™

Derivatize analytes quickly and quantitatively under mild conditions using Thermo Scientific™ BSA Silylation Reagent. This reagent reacts readily with a wide range of compounds, including alcohols, amines, carboxylic acids, phenols, steroids, biogenic amines, and alkaloids to form volatile, stable TMS derivatives for GC.

Tri-Sil TP (TMSI:Pyridine) Reagent Thermo Scientific™

Derivatize hydroxyls compounds, particularly carbohydrates with Thermo Scientific™ Tri-Sil TP (TMSI:Pyridine) Reagent. Comprising TMSI in pyridine, this reagent silylates alcohols and phenols, organic acids, hydroxylamines, amino acids, carbohydrates, flavonoids, glycols and polyglycols, nucleotides, steroids, hydroxyl acids, barbituates, narcotics, indoles, and vitamins.

Perfluoroacylimidazoles (TFAI and HFBI) Acylation Reagents Thermo Scientific™

Conduct acylation of hydroxyl groups and primary and secondary amines with the Thermo Scientific™ Perfluoroacylimidazoles (TFAI and HFBI). Compared to acid chlorides and anhydrides, the reactions of fluorinated acylimidazoles are smooth and quantitative, and produce no acid byproducts. The principal byproduct is imidazole, which is relatively inert. The volatile and stable fluoroacyl derivatives are ideal for FID and electron-capture detection (ECD) techniques.

Tri-Sil TBT (TMSI:BSA:TMCS) Reagent Thermo Scientific™

Employ a catalyzed silylation reagent formulation, for derivatization of hydroxyls with the Thermo Scientific™ Tri-Sil TBT (TMSI:BSA:TMCS) Reagent. Composed of TMSI/BSA/TMCS (3:3:2), this catalyzed silylation reagent formulation typically reacts with hydroxyls in a short period of time at 60–80 °C to form TMS ethers. Highly hindered hydroxyls may require several hours.

Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA) Acylation Reagents Thermo Scientific™

Prepare fluoracyl derivatives for GC/MS and for flame ionization and electron-capture detection techniques with the Thermo Scientific™ Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA). These reagents react readily with alcohols, phenols, and amines.

HMDS (Hexamethyldisilazane) Thermo Scientific™

Thermo Scientific™ HMDS (Hexamethyldisilazane) greatly extends the practical range of GC, improving chromatographic results in the silylation of sugars and related substances.

MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent Thermo Scientific™

Produce stable TBDMS derivatives for GC analysis with the Thermo Scientific™ MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent. MTBSTFA derivatizes hydroxyl, carboxyl, thiol and primary and secondary amines with typically >96% yields, and the reaction byproducts are volatile and neutral. TBDMS ethers are 104 times more stable to hydrolysis than the corresponding TMS ethers. Addition of Thermo Scientific™ TBDMCS increases silylation potential.
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