Recombinant Proteins

Feature Collection

Validated Application

CD274 (PDL1) Recombinant Human Protein, hIgG1-Fc tag Thermo Scientific™

CD274 molecule (CD274), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human CD274 (NP_054862.1) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized human PDCD1/PD1 at 10 µg/ml (100 µg/well) can bind recombinant human CD274/PDL1-Fc chimera with a linear range of 0.02-0.4 µg/ml
.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

PVR (CD155) Recombinant Human Protein, hIgG1-Fc Tag Thermo Scientific™

Poliovirus receptor (PVR/CD155), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 343) of human PVR (NP_006496.3) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized human DNAM1 at 2 µg/ml(100 µl/well) can bind human PVR-Fc with a linear range of 0.032-0.8 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



NGF Recombinant Mouse Protein Thermo Scientific™

Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effec is 0.2-2.0 ng/ml.

Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

CTLA4 Recombinant Mouse Protein, His Tag Thermo Scientific™

Cytotoxic T-Lymphocyte-Associated 4 (CTLA4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 µg/ml (100 µl/well) can mouse CD80-Fc (cat:50446-M02H) with a linear range of 0.78-62.5 ng/ml.
2. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 µg/ml (100 µl/well) can human B7-1-Fc (Cat:10698-H03H) with a linear range of 0.78-125 ng/ml..

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

CD19 Recombinant Human Protein, His tag Thermo Scientific™

Cluster of Differentiation 19 (CD19) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure the study of protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein is expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 291) of human CD19 (AAB60697) fused to a polyhistidine tag at the C-terminus.

N-terminal Sequence Analysis: Pro 20.

Activity: measured by its binding ability in a functional ELISA . Immobilized human CD19 at 2 µg/mL(100 µL/well) has been shown to bind biotinylated human CD9 with a linear range of 0.032-0.8 µg/mL.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Cluster of Differentiation 19 (CD19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulinlike) domains. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. It interacts with VAV. It interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. CD19 is an important pan B cell marker and co-stimulatory protein in humans and mice. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 defines signaling thresholds for the Ag receptor as well as other cell surface receptors that regulate B lymphocyte selection, activation, and differentiation. Defects in CD19 are a cause of hypogammaglobulinemia.

You may also be interested in the following CD19 products:
Cell Isolation
Primary Antibodies
Gene Expression Assays

FCGR2B (CD32b) Recombinant Human Protein, His Tag Thermo Scientific™

Fc gamma receptor II-b (FCGR2B/CD32b), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Ala 46-Pro 217) of mature human FCGR2B (NP_001002274.1) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.

Activity: Measured by its binding ability in a functional ELISA . Immobilized recombinant human FCGR2B at 10 µg/ml(100 µl/well) can bind human IgG2 with a linear range of 0.16-6.4 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

FCGR2A (CD32a) Recombinant Human Protein, His Tag Thermo Scientific™

Fc gamma receptor II-a (FCGR2A/CD32a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human FCGR2A precursor (AAA35827.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human FCGR2A at 10 µg/ml(100 µl/well) can bind human IgG2 with a linear range of 12.5-200 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

CD96 Recombinant Mouse Protein, His Tag Thermo Scientific™

CD96 antigen (CD96), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of mouse CD96 (Q3U0X8) (Met 1-Met 536) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD96 at 10 µg/ml (100 µl/well) can bind mouse PVR with a linear range of 6.4-160 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

PVR Recombinant Human Protein, His Tag Thermo Scientific™

Poliovirus Receptor (PVR), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human PVR isoform a (NP_006496.3) extracellular domain (Met 1-Asn 343) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized human PVR at 2 µg/ml (100 µl/well) can bind human CD226/DNAM1 with a linear range of 1.28-32 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

CD70 Recombinant Human Protein, hIgG1-Fc Tag Thermo Scientific™

Cluster of Differentiation 70 (CD70), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gln 39-Pro 193) of human CD70 (NP_001243.1) fused to the Fc region of human IgG1 at the N-terminus with a polypeptide linker.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized mouse CD27 at 2 µg/ml(100 µl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.
2. Immobilized mouse CD27-Fc at 2 µg/ml(100 µl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

ERBB2 Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag Thermo Scientific™

V-erb-b2 erythroblastic leukemia viral oncogene homolog 2 (ERBB2), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ERBB2 (NP_004439.2) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human ERBB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

CD36 Recombinant Human Protein, His tag Thermo Scientific™

Cluster of Differentiation 36 (CD36) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure the study of protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein is expressed from a DNA sequence encoding the extracellular domain (Gly 30-Asn 439) of human CD36 (NP_001001547.1) fused both to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

N-terminal Sequence Analysis: Asp 31.

Activity: measured by its binding ability in a functional ELISA . Immobilized human CD36 at 20 µg/mL (100 µL/well) has been shown to bind biotinylated human RSPO1 with a linear range of 0.032-4 µg/mL.

Formulation: lyophilized in PBS, pH 7.4, 5% mannitol, 5% trehalose, and 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
CD36, is integral membrane protein found on the surface of many cell types in vertebrate animals. It belongs to the CD36 family and is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 has also been implicated in hemostasis, thrombosis, malaria, inflammation, lipid metabolism and atherogenesis. CD36 seems to have numerous potential physiological functions. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and binds long chain fatty acids. CD36 may also function as a cell adhesion molecule and in the transport and/or as a regulator of fatty acid transport. Defects in CD36 are the cause of platelet glycoprotein IV deficiency also known as CD36 deficiency. As a receptor for thrombospondin 1, CD36 plays a role in the regulation of angiogenesis, which may be a therapeutic strategy for controlling the dissemination of malignant neoplasms.

You may also be interested in the following CD36 products:
Primary Antibodies
Gene Expression Assays

PARP1 Recombinant Human Protein, His Tag Thermo Scientific™

Poly (Adp-Ribose) Polymerase 1 (PARP1), recombinant human protein is supplied in frozen format. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from The amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human PARP1 at 10 µg/ml (100 µl/well) can bind to biotinylated human HSP70. The EC50 of biotinylated human HSP70 is 0.035 µg/ml.

Formulation: 50 mM Tris, 100 mM NaCl, pH 8, 2 mM EDTA, 2 mM PMSF, 0.5 mM TCEP, 10% glycerol.

Reconstitution: No reconstitution needed. Store at -80°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

PDCD1 Recombinant Human Protein, hIgG1-Fc.His Tag Thermo Scientific™

Programmed cell death 1 (PDCD1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 167) of human PDCD1 (NP_005009.2) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized recombinant human PDCD1LG2 at 1 µg/ml (100 µl/well) can bind human PDCD1-Fc chimera with a linear range of 7.8-1000 ng/ml
.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

NGF Recombinant Human Protein Thermo Scientific™

Nerve growth factor (NGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human NGF (NP_002497.2) (Ser 122-Arg 239).

N-terminal Sequence Analysis: Ser 122

Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is typically 0.4-1.0 ng/mL.

Formulation: Lyophilized in 50 mM NaAc, 150 mM NaCl, pH 5.5, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

Background
As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In additon to its neurotropic activities, studies indicated that NGF may also have an important role in the regulation of the immune system. The synthesis of β-NGF in astrocytes is enhanced by various other cytokines including IL-1, TNF-α, PDGF and TGF-β .Defects in β-NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) characterized by degeneration of dorsal root and autonomic ganglion cells, as well as sensory and/or autonomic abnormalities.

You may also be interested in the following NGF products:
Enzyme & Protein Assays
Primary Antibodies
Gene Expression Assays
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