September 2019 New Products

Genomics and Proteomics


Genomics and Proteomics


Cell Line


Final Product Type


Immunoassay Kit Grade


Phenol Red Indicator


Reaction Speed


Species


Target Species Validated


NCAM-1 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human NCAM-1 Simplex ProcartaPlex Kit measures NCAM-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Kallikrein-6 (KLK6) Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human Kallikrein-6 (KLK6) Simplex ProcartaPlex Kit measures kallikrein-6 (KLK6) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

AccuSEQ™ Real-Time PCR Detection Software, version 3.0 Applied Biosystems™

AccuSEQ Real-Time PCR Detection Software, version 3.0, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 0.1-mL, 96-well plates for either Fast or Standard cycling.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

TDP-43 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human TDP-43 Simplex ProcartaPlex Kit measures TDP-43 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

MicroSEQ™ ID Microbial Identification Software, version 3.1.3 Applied Biosystems™

MicroSEQ ID Microbial Identification Software, version 3.1.3, allows you to easily identify and classify bacterial or fungal sequences by comparing them to a validated microbial library (available separately).

Features of MicroSEQ ID Microbial Identification Software include:
• Automatically identify unknown specimens against a validated microbial library of over 3000 microbial sequences
• Run plates directly on Applied Biosystems 3500 Series genetic analyzers
• Equipped with security, audit, and e-signature features to enable 21 CFR Pt. 11 compliance

Confidently identify microbes
The MicroSEQ ID sequence libraries (available separately) consist of rigorously validated bacterial and fungal libraries to ensure accurate identification. The MicroSEQ ID 16S rDNA 500 Microbial Library consists of over 2300 500-bp 16S rDNA sequences, including extensive coverage of gram-negative non-fermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. The entries contain the first 500 bases of the 16S rRNA gene, sufficient to identify bacterial unknowns.

The MicroSEQ ID Fungal Gene Library contains 1300 reference sequences from the D2 region of the Large Subunit (rDNA) of yeast and mold species against which an unknown yeast or mold sequence can be compared in order to identify the organism. The MicroSEQ libraries contain information on correctly identified organisms and quality-controlled sequences derived from type strains of curated culture collections. The libraries also support sequence polymorphisms and include bacterial Category A biothreat agents.

Automatically identify unknown specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The Auto-ID feature in MicroSEQ ID software uses a set of editable parameters to automatically identify unknown specimens. The Results table is displayed in the report with an editable Comments field for additional information or manual identification entries.

Run plates directly
Applied Biosystems 3500 Series genetic analyzer users are able to run plates directly via the MicroSEQ ID software user interface. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ system: a complete, integrated solution
Streamlining every microbial identification step, the MicroSEQ system combines the advantages of MicroSEQ ID software with easy-to-use PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. MicroSEQ ID software also includes security, audit, and electronic signature features, enabling the software to smoothly integrate into your workflow.

SiteClick™ Antibody Azido Modification Kit Invitrogen™

Create a label-ready site-specific azido-modified antibody without lengthy and inefficient genetic modification using the SiteClick Antibody Azido Modification Kit. SiteClick labeling uses enzymes to specifically attach an azido moiety to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain unaltered for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Once azido–modified, a variety of labeled SiteClick sDIBO alkynes are available for attachment to the antibody via Click chemistry at your choice of scale. This provides the flexibility to choose different labels for your antibody depending on your assay.

Features of the SiteClick Antibody Azido Modification Kit:
• Contains everything required to azido-modify any species or isotype of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugate

Learn more about SiteClick labeling technology ›

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

Platinum™ SuperFi II DNA Polymerase Invitrogen™

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

Features of Platinum SuperFi II DNA Polymerase include:
• Exceptional >300X Taq fidelity
• Universal primer annealing at 60°C
• Superior specificity, sensitivity, and yields
• Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

Platinum SuperFi II DNA Polymerase is an engineered enzyme high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications of Platinum SuperFi II DNA Polymerase:
• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR

For increased convenience, we offer Platinum SuperFi II Master Mix with Platinum SuperFi II DNA Polymerase provided in a ready-to-use mixture along with SuperFi II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. Platinum SuperFi II Green PCR Master Mix is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis.

View more information about Platinum SuperFi II DNA Polymerase products ›

CytKick™ Max Extended Cooling Adaptor Invitrogen™

The CytKick Max Extended Cooling Adaptor is designed for use with the CytKick Max Autosampler and the Attune Acoustic Focusing Cytometer. This cooling block maintains samples at 2-15°C for 95-100 minutes while they are being analyzed on the instrument. The cooling adaptor is brought to temperature by placing it in a refrigerator overnight or in a freezer for a minimum of two hours prior to use. If this longer cooling time is not required, please see the CytKick Max Universal Cooling Adaptor, which cools samples for ~65 minutes.

CytKick™ Max Tube Cooling Rack Invitrogen™

The CytKick Max Tube Cooling Rack is designed for use with the CytKick Max Autosampler and the Attune Acoustic Focusing Cytometer. It allows sample uptake from 16 1.5- and 2.0-mL Eppendorf tubes without the need to remove the caps. This cooling rack maintains samples at 2-15°C for 108-120 minutes while they are being analyzed on the instrument. The rack is chilled by placing it in a refrigerator overnight prior to use. For a non-cooling version of this rack, please see the CytKick Max Tube Rack.

FGF-21 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human FGF-21 Simplex ProcartaPlex Kit measures FGF-21 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

CytKick™ Max Universal Cooling Adaptor Invitrogen™

The CytKick Max Universal Cooling Adaptor is designed for use with the CytKick Max Autosampler and the Attune Acoustic Focusing Cytometer. This cooling block maintains samples at 2-15°C for ~65 minutes while they are being analyzed on the instrument. The cooling adaptor is brought to temperature by placing it in a refrigerator overnight or in a freezer for a minimum of two hours prior to use. For a longer cooling time (95-100 minutes), please see the CytKick Max Extended Cooling Adaptor.

MicroSEQ™ ID 16S rDNA 500 Supplemental Library, v2019 Applied Biosystems™

The MicroSEQ ID 16S rDNA 500 Supplemental Library v2019 is a stand-alone library that extends the size of the MicroSEQ ID 16S rDNA 500 Library v2019 to strengthen the identification process when using MicroSEQ ID Microbial Identification Software. The Supplemental library contains 7,138 16s rDNA public sequences of type and non-type strains of bacterial species that underwent the same rigorous validation process as the sequences in the MicroSEQ ID 16S rDNA 500 Library v2019. The Supplemental Library is used when the MicroSEQ ID 16S rDNA 500 Library v2019 results in a percent match lower than the species ID acceptance criteria.

Key features include:
• In-silico validated library with over 7,000 type and non-type strains quality-checked for sequence, taxonomy, nomenclature, and phylogeny
• Reliable entries sourced from public resources converted into a simple format for routine validation of unique environmental isolates

Amyloid Beta 1-40 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human Amyloid beta 1-40 Simplex ProcartaPlex Kit measures amyloid beta 1-40 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample

Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Human OATP1A2 SLC Transporter Cells Gibco™

TRANSiPORT Human OATP1A2 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP2B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1A2 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy-to-use format
• Obtain high-quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock OATP1A2 SLC Transporter Cells (Cat. No. GM1017) that do not overexpress the OATP1A2 SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in two days from thawing of the cells to final results.

Platinum™ Direct PCR Universal Master Mix Invitrogen™

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

Features of Platinum Direct PCR Universal Master Mix include:
• Direct DNA amplification from various samples due to engineered, inhibitor-tolerant Platinum II Taq Hot-Start DNA Polymerase
• Universal primer annealing temperature due to innovative buffer that enables primer annealing at 60°C
• Superior specificity, sensitivity, and yields due to Platinum hot-start technology

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The kit includes optimized reagents to achieve superior results. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments. Samples in the lysis buffer can be stored for later use. Platinum Direct PCR Universal Master Mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

View more information about Platinum Direct PCR Universal Master Mix ›

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