May 2020 New Products

CLA IdentiFiler™ Plus PCR Amplification Kit Applied Biosystems™

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines. It includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition. The kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.

Features of the CLA IdentiFiler Plus PCR Amplification Kit include:
• 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Plus kit
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

OncoStat Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-synthetic nucleosomes bearing distinct histone mutations as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the mutation of interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The OncoStat Panel consists of a pool of nucleosomes harboring seven well-studied histone H3.3 mutations that have been implicated in cancer (histone H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W and H3.3K36M) plus a wild-type H3.3 control. Further, each of these eight nucleosomes is wrapped by two independent DNA barcodes (16 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

Applied Biosystems 3500xL Dx Genetic Analyzer Applied Biosystems™

The Applied Biosystems 3500xL Dx Genetic Analyzer is the first capillary electrophoresis platform for in vitro diagnostic (IVD) use for Sanger sequencing and fragment analysis of DNA. Sanger sequencing and fragment analysis are known as the 'gold-standard' for its accuracy, reliability, and ease of use.

Confidence in your results
You can now feel even more comfortable adopting the 3500xL Dx Genetic Analyzer platform for IVD use for Sanger sequencing and fragment analysis of DNA.
• The 3500xL Dx Genetic Analyzer is an IVD-labeled platform for Sequencing-Based Typing using SeCore HLA typing kits
• High accuracy: 99% concordance as shown in clinical trials using SeCore HLA typing kits
• The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are FDA-cleared for DNA fragment analysis with the AmplideX™ Fragile X Dx and Carrier Screen Kit from Asuragen, Inc.
• Direct injection of PCR products (no PCR clean up) into the Applied Biosystems 3500xL Dx Genetic Analyzer

Simplicity and efficiency
The 3500xL Dx Genetic Analyzer is a partner in your laboratory, making it easy for you to comply with various industry standards and simplifying the training of new users.
• Minimal instrument hands-on time with simplified installation and removal of consumables and accessories
• Controlled and efficient run setup with intuitive user interface, preconfigured plate templates, and software-controlled functions (plate setup, data collection, analysis)
• Instrument run-times as short as 1 hour; sample-to-result in as little as 1.5 days
• Maintenance calendar with programmable prompts and service history
• Security, audit, and electronic signature (SAE), radio frequency identification (RFID) tracking of consumables, built-in quality controls, and real-time data quality evaluation

Flexibility
• Collect data in IVD mode which is validated for sequencing using the SeCore HLA kit and validated for fragment analysis
• Different configurations to meet your throughput needs: 8-capillary (3500 Dx Genetic Analyzer) and 24-capillary (3500xL Dx Genetic Analyzer) versions
• Integrated, dual-mode software for instrument control, data collection, quality control, and auto-analysis of sample files

IVD-labeled accessories and consumables
The purchase of a 3500xL Dx Genetic Analyzer includes a computer system and associated data collection software, as well as an installation kit containing the initial set of accessories and reagents. Installation includes a full install qualification and operational qualification, as well as instrument training.

The 3500 Dx Genetic Analyzer is backed by a comprehensive service and support program with options to purchase additional services depending upon your laboratory maintenance and audit requirements.

Intended use—Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer
The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are in vitro diagnostic devices intended for detection of fluorescently-labeled human genomic deoxyribonucleic acid (DNA) nucleotides by capillary electrophoresis.

The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are indicated for sequencing and fragment analysis using FDA- cleared or approved assays.

Intended Use—AmplideX Fragile X Dx & Carrier Screen Kit
The AmplideX Fragile X Dx & Carrier Screen Kit is an in vitro diagnostic device that uses polymerase chain reaction (PCR) and capillary electrophoresis to detect and identify the number of cytosine-guanine-guanine (CGG) repeats in the fragile X mental retardation-1 (FMR1) gene using genomic DNA isolated from peripheral whole blood specimens. It is solely intended as an aid in the post-natal diagnosis of fragile X syndrome, and fragile X-associated disorders [i.e., fragile X-associated tremor/ataxia syndrome (FXTAS) or fragile X-associated primary ovarian insufficiency (FXPOI)], and for carrier testing in adults of reproductive age. Assay results are solely intended to be interpreted by healthcare professionals who are board certified in molecular genetics and to be used in conjunction with other clinical and diagnostic findings, consistent with professional standards of practice. Reflex testing, clinical genetic evaluation, and genetic counseling should be offered as appropriate. The test is for use on the Applied Biosystems 3500 Dx Series Genetic Analyzers.

This test is not indicated for use for fetal diagnostic testing, newborn screening, or for stand‐alone diagnostic purposes.

Note: The 3500xL Dx Genetic Analyzer data collection software uses the Windows 10 operating system.

UltraComp eBeads™ Plus Compensation Beads Invitrogen™

UltraComp eBeads Plus Compensation Beads are the second generation of UltraComp beads for use in flow cytometry. Fluorochrome-conjugated antibody binds the spherical particles for use as a single-color compensation controls. Like the first generation beads, they bind with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers. Unlike the first generation beads, UltraComp eBeads Plus compensate in a manner similar to cells when used with Brilliant Violet 785/786-, Brilliant Violet 711-, Super Bright 780- and Super Bright 702-conjugated antibodies.

Features include:
• Multispecies compatibility—bind with mouse, rat, hamster, rabbit and recombinant human antibodies
• Improved fluorophore compatibility

Each drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed. This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.

Ion AmpliSeq™ CarrierSeq™ ECS Panel Ion Torrent™

The Ion AmpliSeq CarrierSeq ECS Panel enables detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay. It is included in the Ion Torrent CarrierSeq ECS kits, which provide all of the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.

Benefits of the Ion AmpliSeq CarrierSeq ECS Panel include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Increased carrier status detection rate through genotyping of SNV, indel, and CNV carrier status by NGS
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.
vGenetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

K-MetStat Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded semi-syntheic nucleosomes bearing distinct post-translational as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the post-translational modification of interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The K-MetStat Panel is a lysine methylation status panel that consists of a pool of nucleosomes carrying fifteen well-studied, disease relevant lysine methyl marks on histones H3 and H4 (mono-, di- and tri-methylated H3K4, H3K9, H3K27, H3K36, and H4K20) plus an unmodified control. Further, each of these nucleosomes is wrapped by two independent DNA barcodes (32 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

RiboMinus™ Pan-Prokaryote Probe Mix Invitrogen™

RiboMinus Pan-Prokaryote Probe Mix is a component of the RiboMinus Bacteria 2.0 Transcriptome Isolation Kit and is available as a standalone product for use in conjunction with other RiboMinus products targeting other species: Plant (Cat. No. A1083808) and Eukaryote v2 (Cat. No. A15026). Combining RiboMinus Pan-Prokaryote Probe Mix with Plant or Eukaryote probe mixes is useful for metatranscriptomic studies. This probe mix consists of over 150 3’-biotin-labeled ssDNA oligonucleotide designs. These designs were created using highly conserved regions of rRNA across 76 pan-prokaryote species (see table below) and tested with several species (see compatibility list below).

RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit Invitrogen™

Highly efficient depletion of 16S, 23S, and 5S rRNA from a variety of species
The RiboMinus Bacteria 2.0 Transcriptome Isolation Kit is a complete and automatable kit that enables efficient and selective removal of bacterial and archaebacterial ribosomal RNA (rRNA) from intact total RNA (100 ng to 5 μg) in less than two hours.

Key features of the RiboMinus Bacteria 2.0 kit:
Efficient—the included RiboMinus Pan-Prokaryote Probe Mix has consistently shown less than 10% of reads mapping to 16S and 23S rRNA reads
Wide range of targets—designed with >150 probe designs targeting 16S, 23S, and 5S rRNA targets from >76 representative species of bacteria and archaebacteria from common phyla and sub-phyla
Convenient—Dynabeads MyOne Streptavidin C1 magnetic beads and bead cleanup module and protocols included for Kingfisher instruments
Flexible— accommodates mass inputs from 100 ng to 5 µg of total RNA; works with wide range of total RNA samples with RIN values from 5.0–10.0
High detection sensitivity—increases the number of sequencing reads mapping to protein-coding transcripts compared to previous version
Suitable for metatranscriptomics—deplete rRNA of many microbial species in metagenomic samples

Target rRNA from panorama of bacteria and archaebacteria
The RiboMinus Bacteria 2.0 kit is based on hybridization-capture technology and includes a new RiboMinus Pan-Prokaryote Probe Mix, which results in a 4-fold decrease of ribosomal reads remaining and shows significant improvement over the previous bacterial probe mix. The new probe mix consists of over 150 3’-biotin-labeled ssDNA oligonucleotiode designs. These designs were created using highly conserved regions of rRNA across 76 pan-prokaryote species (see table below) and tested with several species (see compatibility list below).

Dynabeads MyOne Streptavidin C1 cleanup kit included
Once the probe mix has selectively hybridized to the target rRNA transcripts, removal of the probe-rRNA complex is completed by capture on Dynabeads MyOne Streptavidin C1 magnetic beads, leaving behind rRNA-depleted RNA. The RiboMinus Bacteria 2.0 kit includes a magnetic bead-based cleanup module for concentration of the rRNA-depleted RNA, enabling easier handling, scalability, and automation on KingFisher instruments. The resulting rRNA-depleted RNA (RiboMinus RNA) is suitable for whole-transcriptome analysis by RNA-Seq or other downstream applications.

Compatiblity
The RiboMinus Pan-Prokaryote Probe Mix has been shown to be compatible with the following tested species:
Acinetobacter baumannii
Bacteroides thetaiotaomicron
Bifidobacterium longum subsp. infantis.
Campylobacter jejuni
Clostridioides difficile
Escherichia coli
Fusobacterium nucleatum
Helicobacter pylori
Janthinobacterium sp.
Moorella thermoacetica
Pseudomonas putida
Rickettsia rickettsia
Salmonella typhimurium
Pseudomonas aeruginosa
Salmonella enterica
Staphylococcus aureus
Thermoanaerobacter kivui
Vibrio cholerae
Vibrio fisheri

The RiboMinus Pan-Prokaryote Probe Mix has been shown to be compatible with the following species based on sequence homology:
Corynebacterium
Enterococcus faecalis
Gluconobacter
Myxococcus xanthus
Streptomyces coelicoflavus

Applied Biosystems 3500 Dx Genetic Analyzer Applied Biosystems™

The Applied Biosystems 3500 Dx Genetic Analyzer is the first capillary electrophoresis platform for in vitro diagnostic (IVD) use for Sanger sequencing and fragment analysis of DNA. Sanger sequencing and fragment analysis are known as the 'gold-standard' for its accuracy, reliability, and ease of use.

Confidence in your results
You can now feel even more comfortable adopting the 3500 Dx Genetic Analyzer platform for IVD use for Sanger sequencing and fragment analysis of DNA.
• The 3500 Dx Genetic Analyzer is an IVD-labeled platform for Sequencing-Based Typing using SeCore HLA typing kits
• High accuracy: 99% concordance as shown in clinical trials using SeCore HLA typing kits
• The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are FDA-cleared for DNA fragment analysis with the AmplideX™ Fragile X Dx and Carrier Screen Kit from Asuragen, Inc.
• Direct injection of PCR products (no PCR clean up) into the Applied Biosystems 3500 Dx Genetic Analyzer

Simplicity and efficiency
The 3500 Dx Genetic Analyzer is a partner in your laboratory, making it easy for you to comply with various industry standards and simplifying the training of new users.
• Minimal instrument hands-on time with simplified installation and removal of consumables and accessories
• Controlled and efficient run setup with intuitive user interface, preconfigured plate templates, and software-controlled functions (plate setup, data collection, analysis)
• Instrument run-times as short as 1 hour; sample-to-result in as little as 1.5 days
• Maintenance calendar with programmable prompts and service history
• Security, audit, and electronic signature (SAE), radio frequency identification (RFID) tracking of consumables, built-in quality controls, and real-time data quality evaluation

Flexibility
• Collect data in IVD mode which is validated for sequencing using the SeCore HLA kit and validated for fragment analysis
• Different configurations to meet your throughput needs: 8-capillary (3500 Dx Genetic Analyzer) and 24-capillary (3500xL Dx Genetic Analyzer) versions
• Integrated, dual-mode software for instrument control, data collection, quality control, and auto-analysis of sample files

IVD-labeled accessories and consumables
The purchase of a 3500 Dx Genetic Analyzer includes a computer system and associated data collection software, as well as an installation kit containing the initial set of accessories and reagents. Installation includes a full install qualification and operational qualification, as well as instrument training.

The 3500 Dx Genetic Analyzer is backed by a comprehensive service and support program with options to purchase additional services depending upon your laboratory maintenance and audit requirements.

Intended use—Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer
The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are in vitro diagnostic devices intended for detection of fluorescently-labeled human genomic deoxyribonucleic acid (DNA) nucleotides by capillary electrophoresis.

The Applied Biosystems 3500 Dx Genetic Analyzer and the Applied Biosystems 3500xL Dx Genetic Analyzer are indicated for sequencing and fragment analysis using FDA- cleared or approved assays.

Intended Use—AmplideX Fragile X Dx & Carrier Screen Kit
The AmplideX Fragile X Dx & Carrier Screen Kit is an in vitro diagnostic device that uses polymerase chain reaction (PCR) and capillary electrophoresis to detect and identify the number of cytosine-guanine-guanine (CGG) repeats in the fragile X mental retardation-1 (FMR1) gene using genomic DNA isolated from peripheral whole blood specimens. It is solely intended as an aid in the post-natal diagnosis of fragile X syndrome, and fragile X-associated disorders [i.e., fragile X-associated tremor/ataxia syndrome (FXTAS) or fragile X-associated primary ovarian insufficiency (FXPOI)], and for carrier testing in adults of reproductive age. Assay results are solely intended to be interpreted by healthcare professionals who are board certified in molecular genetics and to be used in conjunction with other clinical and diagnostic findings, consistent with professional standards of practice. Reflex testing, clinical genetic evaluation, and genetic counseling should be offered as appropriate. The test is for use on the Applied Biosystems 3500 Dx Series Genetic Analyzers.

This test is not indicated for use for fetal diagnostic testing, newborn screening, or for stand‐alone diagnostic purposes.

Note: The 3500 Dx Genetic Analyzer data collection software uses the Windows 10 operating system.

Olympus™ 60X Objective, fluorite, 0.90NA/0.2WD, correction collar (0.11–0.23 mm) Invitrogen™

This fluorite (semi-apochromat) objective is equipped with an adjustable collar that affords flexibility in imaging through vessels with a thickness from 0.11 to 0.23 mm. With four-color correction for spherical and chromatic aberrations, it delivers exceptional image quality. For the sharpest image simply set the collar to the thickness of the vessel/coverslip you are using. If the thickness is unknown, turn the collar in either direction while monitoring the sample focus. All Olympus objectives are compatible with EVOS cell imaging systems and offer outstanding optical performance from visible to near-infrared light. The extensive selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:
• Magnification: 60X
• Numerical Aperture: 0.90
• Working Distance: 0.2 mm
• Superb image quality

Image quality
Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation. High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide.
Explore the entire EVOS line of imaging systems and accessories ›

CarrierSeq™ ECS Kit with Ion 540™ Chips (15 samples/chip) Ion Torrent™

The Ion Torrent CarrierSeq ECS kits provide all the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.The Ion AmpliSeq CarrierSeq ECS Panel included in the kits enables the detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay.

Benefits of the CarrierSeq ECS kits include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))
• For use with Carrier Reporter, intuitive data analysis software that quickly translates data into results with customizable analysis and reporting options
• Flexible throughput options—kit formats available for 3, 4, 15, or 16 samples per chip or 6, 8, 30, or 32 samples per run

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.

Genetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

Streamlined implementation and workflow
The CarrierSeq ECS kits are an end-to-end solution that includes reagents for target amplification, library preparation, and sequencing with software for data analysis and reporting. The reagents are optimized to work together out of the box and ready to use with the Ion Chef and Ion GeneStudio S5 systems for template preparation and sequencing, respectively, and Carrier Reporter Software for reporting functionality with customizable options.

Straight-forward and powerful data analysis and reporting
Carrier Reporter Software is easy to use and designed specifically for carrier screening analysis and reporting. It incorporates the output of special analysis algorithms that address CNV and SNV/indel calling for technically challenging genes such as CYP21A2, GBA, HBA1/2, and SMN1.

K-AcylStat™ Panel, SNAP-ChIP™ Spike-in Invitrogen™

SNAP-ChIP (Sample Normalization and Antibody Profiling Chromatin ImmunoPrecipitation), a proprietary technology from EpiCypher, uses DNA-barcoded designer nucleosomes bearing distinct post-translational modifications as spike-in ChIP controls.

• Homogenous, fully defined standards faithfully represent target mononucleosomes in the experimental sample
• Nucleosomes are subjected to rigorous quality control for lot-to-lot consistency
• Unique DNA barcodes can be distinguished from experimental sample genomes
• Spike-ins provide a direct readout of antibody performance in ChIP
• Ability to monitor technical variability between samples
• Defined standards enable universal normalization across experiments

SNAP-ChIP panels are directly compatible with your current ChIP workflow, with semi-synthetic nucleosomes bearing the post-translational modification interest immunoprecipitated and processed alongside sample chromatin.

Users can directly monitor antibody capability (specificity and enrichment) in a ChIP experiment by measuring the recovery of on- and off-target nucleosomes. The SNAP spike-ins can be further used as a defined standard to normalize your ChIP data, thereby controlling for technical variability across experiments.

The K-AcylStat Panel is a lysine acylation status panel that consists of a pool of nucleosomes carrying twenty-two well-studied, disease relevant lysine acylation marks on histones H2A, H3, and H4 (see user manual for specifics) plus an unmodified control. Further, each of these nucleosomes is wrapped by two independent DNA barcodes (46 total barcodes in the panel), allowing for an internal technical replicate in each ChIP reaction. A single spike-in of the panel allows users to check antibody specificity by examining the post-IP recovery of on- versus off-target SNAP-ChIP nucleosomes, supporting the generation of high quality ChIP data.

NGS Reverse Transcription Kit Ion Torrent™

The Ion Torrent NGS Reverse Transcription (RT) Kit is the first cDNA synthesis kit developed specifically for next-generation sequencing (NGS) applications. It combines the superior performance of SuperScript IV Reverse Transcriptase with a novel master mix optimized for NGS library preparation. The NGS RT Kit is compatible with Ion AmpliSeq and Ion AmpliSeq HD assays and can be used with both manual and automated workflows.

Benefits of the NGS RT Kit include:
• Optimized for a variety of sample types, including FFPE, degraded samples, and those with low template amounts
• Superior NGS results with novel RT formulation optimized for NGS library preparation
• Helps increase NGS library yield, improve detection of long amplicons, and rescue under-performing amplicons
• Helps maximize mapped reads and improve detection of RNA fusions
• Trusted SuperScript IV RT technology offers superior cDNA synthesis performance with even the most challenging RNA samples
• Universal kit for wide variety of Oncomine oncology research assays and Ion AmpliSeq panels

Simple reaction setup
The NGS RT Kit contains a tube of 10X SuperScript IV enzyme mix and a tube of 5X buffer. A one-step, 25-minute reaction reverse transcribes total RNA into cDNA and can be used directly for NGS library preparation, without any additional quantification, purification, or dilution steps. A variety of sample types can be used, including RNA from blood, bone marrow, FFPE and partially degraded samples. This product is compatible with Oncomine and Ion AmpliSeq workflows, as well as Ion AmpliSeq HD panels. Consult the cDNA synthesis section of each assay manual for specific RT reaction volumes and setup instructions.

Performance and quality testing
The NGS RT Kit is assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product. It is QC tested with a full NGS workflow to evaluate library yield and ensure performance meets key sequencing metrics.

CarrierSeq™ ECS Kit with Ion 530™ Chips (3 samples/chip) Ion Torrent™

The Ion Torrent CarrierSeq ECS kits provide all the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.The Ion AmpliSeq CarrierSeq ECS Panel included in the kits enables the detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay.

Benefits of the CarrierSeq ECS kits include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))
• For use with Carrier Reporter, intuitive data analysis software that quickly translates data into results with customizable analysis and reporting options
• Flexible throughput options—kit formats available for 3, 4, 15, or 16 samples per chip or 6, 8, 30, or 32 samples per run

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.

Genetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

Streamlined implementation and workflow
The CarrierSeq ECS kits are an end-to-end solution that includes reagents for target amplification, library preparation, and sequencing with software for data analysis and reporting. The reagents are optimized to work together out of the box and ready to use with the Ion Chef and Ion GeneStudio S5 systems for template preparation and sequencing, respectively, and Carrier Reporter Software for reporting functionality with customizable options.

Straight-forward and powerful data analysis and reporting
Carrier Reporter Software is easy to use and designed specifically for carrier screening analysis and reporting. It incorporates the output of special analysis algorithms that address CNV and SNV/indel calling for technically challenging genes such as CYP21A2, GBA, HBA1/2, and SMN1.

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