June 2020 New Products

Feature Collection

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Cell Type


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Final Product Type


For Use With (Application)


Height (Metric)


Label or Dye


Length (Metric)


No. of Samples


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Product Type


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Second Strand cDNA Synthesis Kit Invitrogen™

Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

Celleste™ 5.0 Image Analysis Software, network server license + 1 seat Thermo Scientific™

Celleste 5.0 Image Analysis Software has features designed for a range of biological applications, such as image adjustment and processing with manual and automatic measurements over multiple channels, and segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste software allows you to seamlessly capture, process, measure, analyze, and share images and data.

This Network Server License is designed for environments where the use of multiple copies is desired. It comes with one seat; additional seats can be added at any time.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Comprehensive image processing and enhancement functions with optional modules to meet your specific needs
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• A new interface for streamlined multi-well plate navigation and well selection
• A wide range of functions to create reports and share images and data

Process and analyze
After image acquisition, immediately apply a suite of image processing and enhancement functions with a few clicks of a mouse. Use auto-alignment for overlays and remove background to reveal critical details with a wide range of filters. Once satisfied with the results from one image, apply the same steps to a set of time-lapse images, scans, or results from different treatments. When imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

Measure and quantify
Easily measure and analyze your images with a variety of measurement tools, such as those for distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. This feature is particularly powerful in combination with the EVOS Onstage Incubator, which allows continuous monitoring of cells under controlled environmental conditions.

Count and classify
Using a number of manual and automatic measurement tools, easily count and characterize cells and subcellular objects based on area, length, shape, and light intensity. Flexible segmentation tools allow you to label images based on color or intensity level. A new ROI functionality allows you to count objects and maintain measurements by region (e.g., a parent-child application) and also to count by ROI. After object counting, sort and display counted images based on size and other parameters.

Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready image and data reports with a few clicks and to share them with others in PDF, PowerPoint, and Excel format.

Celleste 5.0 software is a powerful image analysis solution that helps make your imaging research job easier and increase your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS microscopes, you now have the tools to make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.

HO-1 (Heme oxygenase 1) Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human HO-1 (Heme oxygenase 1) Simplex ProcartaPlex Kit measures HO-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

imPULSE™ Single-Use Mixer (S.U.M.) Thermo Scientific™

The Thermo Scientific imPULSE Single-Use Mixer (S.U.M.) can be used for many bioprocess mixing applications. The imPULSE S.U.M. design features include innovative disc-mixing technology, configurable high-end controls, and monitors to fit specific process requirements. These features enable uniform, superior mixing that is scalable from 30 L to 5,000 L. The touchscreen console for the imPULSE S.U.M. provides unsurpassed integrated sensor monitoring and pump control for pH and saline titration and for automatic fill and harvest of the S.U.M.

imPULSE S.U.M features include:
• Touchscreen console provides ease of use with multi-functional capabilities to monitor and control mixing parameters
• Integrated rolling diaphragm that provides the pumping action to the mixing disc; the diaphragm will not abrade the surfaces or produce particulate
• Mixing tank jacket and insulation
• Load cell weighing systems enable accurate batch weight monitoring
• Auto inflate and vent control options
• Adjustable powder hanger for 1,000 L and higher mixers that fits 1, 5, and 25 kg Powdertainer BPCs
• Open cart frame for easier cleaning

Applications:
• Media preparation
• Final formulation steps
• Buffer preparation
• Use as a harvest vessel
• Pooling and liquid transfer
• Product suspension
• Mixing and storage of multiple batches
• Viral inactivation

High-Intensity Perfusion CHO Medium Thermo Scientific™

Gibco High-Intensity Perfusion (HIP) CHO Medium is an innovative, chemically defined, animal origin-free (AOF), cGMP medium. This easy-to-use perfusion medium helps enable high titers in numerous CHO cell lines from the beginning to the end of the process regardless of the type of perfusion platform, all while using less medium.

Features of HIP CHO Medium include:
• Tested with CHO-S, CHO-DG44, CHO-GS, and CHO-K1 cell lines
• Supports high cell density of over 100 million cells/mL at 1 vessel volume/day (VVD) continuous perfusion
• Demonstrated production of up to 1.7 g/L/day at 1 VVD continuous perfusion

Flexibility
HIP CHO Medium can be used at multiple concentrations to support seed train to production with one product. The flexible reconstitution of the medium allows dynamic tailoring of medium concentration to meet process and clone needs. Just as it can be used at multiple concentrations, HIP CHO Medium can be used in multiple perfusion processes: N-1 and seed train, intensified fed-batch, concentrated fed-batch, and continuous perfusion.In addition, HIP CHO Medium supports high cell densities at low medium exchange rates (as low as 1 VVD), which improves process efficiency.

HIP CHO Medium can help enable your move toward perfusion processes, which in turn can lead to a more compact and efficient workflow while improving productivity and quality.

Applications of HIP CHO Medium:
• Suspension CHO cells
• Selection system such as glutamine synthetase (GS) and dihydrofolate reductase (DHFR)
• Passaging, seed train, and protein production applications
• Optimized for use without feeds in perfusion application (other than glucose)

AFP (Alpha-fetoprotein) Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human AFP (Alpha-fetoprotein) Simplex ProcartaPlex Kit measures AEP protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Celleste™ 5.0 Image Analysis Software, additional seat Thermo Scientific™

Celleste 5.0 Image Analysis Software has features designed for a range of biological applications, such as image adjustment and processing with manual and automatic measurements over multiple channels, and segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste software allows you to seamlessly capture, process, measure, analyze, and share images and data.

This Network Seat is designed for environments where the use of multiple copies is desired. It requires an active Celleste 5.0 Network Server License.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Comprehensive image processing and enhancement functions with optional modules to meet your specific needs
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• A new interface for streamlined multi-well plate navigation and well selection
• A wide range of functions to create reports and share images and data

Process and analyze
After image acquisition, immediately apply a suite of image processing and enhancement functions with a few clicks of a mouse. Use auto-alignment for overlays and remove background to reveal critical details with a wide range of filters. Once satisfied with the results from one image, apply the same steps to a set of time-lapse images, scans, or results from different treatments. When imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

Measure and quantify
Easily measure and analyze your images with a variety of measurement tools, such as those for distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. This feature is particularly powerful in combination with the EVOS Onstage Incubator, which allows continuous monitoring of cells under controlled environmental conditions.

Count and classify
Using a number of manual and automatic measurement tools, easily count and characterize cells and subcellular objects based on area, length, shape, and light intensity. Flexible segmentation tools allow you to label images based on color or intensity level. A new ROI functionality allows you to count objects and maintain measurements by region (e.g., a parent-child application) and also to count by ROI. After object counting, sort and display counted images based on size and other parameters.

Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready image and data reports with a few clicks and to share them with others in PDF, PowerPoint, and Excel format.

Celleste 5.0 software is a powerful image analysis solution that helps make your imaging research job easier and increase your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS microscopes, you now have the tools to make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.

Axiom™ Human Genotyping SARS-CoV-2 Research Array, 96 format Thermo Scientific™

The Axiom Human Genotyping SARS-CoV-2 Research Array is a comprehensive whole-genome genotyping tool designed for epidemiological research of host genetics in relation to SARS-CoV-2 infectious disease. The array offers >820,000 markers prioritized for dense coverage of genes implicated in underlying conditions that may influence disease susceptibility, severity, and outcome, including coronary artery disease, chronic obstructive pulmonary disease, diabetes, and high blood pressure. The array also offers dense coverage of the ACE2 and TMPRSS2 genes associated with viral entry points, as well as coverage of relevant pharmacogenes.

The Axiom Human Genotyping SARS-CoV-2 Research Array, along with the Axiom Genotyping Solution, delivers an impactful research solution that converts samples to whole-genome data for multi-ethnic populations. The array can be used to perform both prospective studies and retrospective studies with previously generated data.

Features of the Axiom Human Genotyping SARS CoV-2 Research Array include:
• Large GWAS module (>820,000) that includes variants for the research of key underlying health conditions for association studies and meta-analyses of previously generated data
• Research markers associated with underlying conditions such as lung disease, diabetes, cardiovascular disease, and respiratory distress
• A SARS-CoV-2-specific research module that includes markers for:
    --Various cytokines and pathway (e.g., NOTCH signaling pathways, RAAS ) associated with immunology, inflammation, and respiratory distress
    --ACE2 and TMPRSS2 genes associated with cell surface receptors and virus entry facilitators
    --Structural and non-structural proteins predicted or observed to interact with viral proteins
• Pharmacogenomic markers for research into druggable targets and adverse drug reactions
• Variants covering various blood groups including ABO
• Over 18,000 markers from the UK Biobank Axiom Array for the research of cardio-vascular disease and diabetes

Required products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software
Axiom Long Export Format Software

Galectin-1 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human Galectin-1 Simplex ProcartaPlex Kit measures Galectin-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

HyPerforma™ Smartainer™ 3.0 Bioprocess Container (BPC), Aegis 5-14 film Thermo Scientific™

This bioprocess container (BPC) is for use with the Thermo Scientific HyPerforma Smartainer 3.0, which provides end-users producing biologics or vaccine therapeutics with a large-volume, sterile liquid storage system for volumes ranging from 200 L to 3000 L. The Smartainer 3.0 system is an update to the Smartainer 2.0 system, and is available in either non-jacketed or jacketed configuation. The Smartainer 3.0 system is designed for the collection and storage of sterile process liquids such as cell culture media, process water, or purification media—wherever large volumes of sterile process liquids need to be safely, securely stored.

BPCs to optimize performance
Choose from a variety of off-the-shelf BPCs or customize a BPC to fit your Smartainer 3.0 system. Customization options include:
• Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
• Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, steam-to and steam-through connectors
• Filters—used for bioburden reduction; sterilization grade filters and vent filters
• Tubing—thermoplastic elastomers, platinum-cured silicone
• Sample ports—septum-style and needle-free ports
• Clamps—sliders, pinch, and crimp bands

imPULSE™ Single-Use Mixer BioProcess Container (BPC) Thermo Scientific™

The Thermo Scientific imPULSE Single-Use Mixer (S.U.M.) BioProcess Container (BPC) is a 3D system composed of ASI 26/77 polyethylene film. The innovative disc design and the hexagonal shape of the disc and vessel are at the core of the quick and thorough mixing capabilities of the imPULSE mixer. ImPULSE BPCs help to eliminate post-use cleaning steps required with reusable containers, reduce cross-contamination risks, and are designed to fit the full range of support containers, both square and cylindrical.

Applications:
• Media preparation
• Final formulation steps
• Buffer preparation
• Use as a harvest vessel
• Pooling and liquid transfer
• Product suspension
• Mixing and storage of multiple batches
• Viral inactivation

Customize standard BPC systems for optimized single-use technology
Choose from one of the industry’s largest component libraries with over 20,000 unique components that include:
Fittings—straight, reducing, elbow, T-style, X-style, and cross-style
Connectors—quick connectors/disconnectors, aseptic connectors and disconnectors, and steam-to and steam-through connectors
Filters—used for bioburden reduction; sterilization grade and vent filters
Tubing—thermoplastic elastomers, platinum-cured silicone
Sample ports—septum-style and needle-free ports
Clamps—sliders, pinch, and crimp bands

N-Cadherin Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human N-Cadherin Simplex ProcartaPlex Kit measures N-Cadherin protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

CD66f (PSBG-1) Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human CD66f (PSBG-1) Simplex ProcartaPlex Kit measures CD66f protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Maxima H Minus Double-Stranded cDNA Synthesis Kit Thermo Scientific™

Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA. First- and second-strand cDNA synthesis reactions are performed in the same tube without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains pre-mixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Maxima H Minus Double-Stranded cDNA Synthesis Kit include:
• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in less than two hours
• Convenient—pre-mixed components
• Complete—includes all primers, controls and residual RNA removal reagents

Applications
• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction

ADAM12 Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human ADAM12 Simplex ProcartaPlex Kit measures ADAM12 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Features include:
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

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