October 2020 New Products

Feature Collection

Capacity


Emission


Excitation


For Use With (Application)


Max. RCF


Max. Speed


Number of Arrays


Refrigerated


Axiom™ PharmacoFocus™ Assay Core Kit, mini 96-array Applied Biosystems™

The Axiom PharmacoFocus Assay Core Kit offers comprehensive coverage of targeted high-evidence pharmacogenomic research content (Pharmacogenomics Knowledge Base annotation levels of evidence 1A-2B) with a cost-efficient and high-throughput solution. The panel includes 2,000 variants in 150 genes and variants in regions of high homology to pseudo-genes (such as cytochrome P450 (CYP) genes), covers population diversity, and offers high accuracy and reproducibility.

The assay offers genotyping, copy number, and HLA typing, achieving the task of three different technologies in a single workflow at a cost per sample that enables profiling of hundreds to hundreds of thousands of samples. The assay is ideal for pharmacogenomic research in labs, academic hospitals, and health care centers, and for pharmaceutical companies with research applications in clinical trials.

Each Axiom PharmacoFocus Assay Core Kit, mini 96-array, includes all necessary array plates, reagents, controls, and consumables to process 94 samples and 2 controls. For a kit that offers star allele reporting with predicted gene phenotype, please see the Axiom PharmacoFocus Assay Kit, mini 96-array.

Features of the Axiom PharmacoFocus Assay Core Kit include:
• 2,000 markers in 150 genes of known pharmacogenomic value
• 88% of markers with PharmGKB annotation levels 1A-2B
• Accurate genotyping of markers in regions of high homology within CYP2C19, CYP2C8, CYP2D6, CYP2A6, CYP2B6, GSTM1, and SULT1A1
• Copy number variation (CNV) analysis for key ADME genes: CYP2D6, CYP2A6, GSTM1, GSTT1, UGB2B17, and SULT1A1
• HLA analysis with high resolution typing (2- and 4-digit) of the major histocompatibility complex (3 MHC Class I and 8 Class II loci) including HLA-A* and HLA-B* types with relevance in drug metabolism

The Axiom PharmacoFocus Assay Core Kit includes:
• Pharmacogenomic markers with highest PharmGKB levels of evidence (levels 1A, 1B, 2A, 2B) accessed in Nov 2019. Majority of annotations are associated with toxicity/adverse drug reactions and efficacy.
• Dutch Pharmacogenetics Working Group passport markers (accessed in Nov 2019)
• OpenArray TaqMan panels markers
• Markers referenced in the Center for Medicare and Medicaid (CMS) reimbursement policy including Psychiatry panel
• Human leukocyte antigen (HLA) markers associated with drug reactions

The Axiom PharmacoFocus Assay Core Kit requires the Applied Biosystems GeneTitan Multi-Channel Instrument, a platform used worldwide by genetic researchers and known for an efficient workflow and high reproducibility. Axiom arrays are manufactured using photolithographic manufacturing technology known for 100% fidelity, ensuring all markers are available in any manufacturing batch.

The Axiom PharmacoFocus Assay Core Kit data is analyzed with Applied Biosystems Axiom Analysis Suite software, which offers genotyping and copy number analysis. The Axiom HLA Analysis software can also be used for HLA typing.

QuantStudio™ 7 Pro Real-Time PCR System, TaqMan™ Array Card, laptop Applied Biosystems™

The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, automation capabilities, and large capacitive touch-screen interface, the QuantStudio 7 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, high throughput, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

Features of the QuantStudio 7 Pro Real-Time PCR System include:
• Easy and convenient hands-free operation with facial authentication and voice commands
• Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
• Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
• Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
• Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
• Application versatility with easily changed blocks and four different block types
• Increased productivity with automation compatibility for 24/7 operation
• Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
• Remote monitoring of run status using the Instrument Connect monitoring app
• Design and Analysis software compatible with PC and Mac operating systems
• Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments

Easy and convenient hands-free operation
The QuantStudio 7 Pro system was designed for a simple and powerful qPCR experience. Hands-free operation with facial authentication and voice commands enables users to quickly and easily access their personal profiles and settings without even removing their gloves.

Smart qPCR for ultimate ease of use
With embedded RFID readers, the QuantStudio 7 Pro system has the capability to run TaqMan Array Plates with RFID tags. These RFID tags simplify data tracking (with embedded lot number, expiration date, etc.) and reduce the need for manual data entry with preloaded assay layout, dye information, and thermal protocol. Automating this data transfer allows users to simply load the plate and start the run—no data entry needed.

Learn more about the TaqMan Array Plates with RFID ›

Peace of mind with Smart service
Minimize downtime with premium service offerings for the QuantStudio 7 Pro system. Smart Help allows users to contact Technical Support directly from the touchscreen, without the need to download files or spend time on the phone. Experienced technicians will analyze the request and respond according to the user’s preference (phone or email) and resolve the issue as quickly as possible.

Smart Remote Support is an immersive collaboration tool that allows customers to quickly and efficiently show issues to Technical Support for resolution, helping reduce instrument downtime compared to on-site repair and helping minimize the need for on-site visits.

Connect
The Connect platform provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the platform are web browser-based software tools that are flexible, fast, and easy to use. These software tools support a variety of analyses including relative quantitation, standard curve, high resolution melt (HRM), genotyping, and presence/absence. They are compatible with Mac or PC, as well as most Applied Biosystems instrument file types.

Format flexibility
The QuantStudio 7 Pro system accommodates the interchange of 96-well (0.2 mL), 96-well (0.1 mL), 384-well, and TaqMan array card (TAC) blocks. The system is delivered with one block type; other block types may be purchased separately. In addition, an Orbitor Microplate Mover is available for this system to support high-throughput workflows. For a more value-priced system that does not support TaqMan array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio 6 Pro Real-Time PCR System. And for the ultimate in flexibility, consider the QuantStudio 12K Flex Real-Time PCR System, which also accommodates TaqMan OpenArray plates.

Accomplish more, faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan Array 384-well microfluidic cards. And when integrated with the Orbitor Microplate Mover (available separately), the QuantStudio 7 Pro system maximizes your throughput capabilities.

High application versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, and gene regulation.

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex system, which features six decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

QuantiGene™ Singleplex Assay Kit Invitrogen™

QuantiGene Singleplex assays are hybridization-based assays for the direct quantitation of RNA, DNA, or microRNA transcripts directly from cell lysates, tissue homogenates or purified RNA. They utilize branched DNA technology for signal amplification, and signal is detected using a standard luminometer. The QuantiGene Singleplex Assay Kit contains all buffers, plates, and detection reagents necessary to perform the assay in 96-well plates. Sample processing kits and target-specific probe sets are sold separately.

The QuantiGene Singleplex Assay is comprised of three modules:
• QuantiGene Singleplex Assay Kit
• QuantiGene Singleplex target-specific probe sets (sold separately)
• QuantiGene Sample Processing Kit (sold separately) (not required for purified RNA samples)

Features of the QuantiGene Singleplex Assay include:
• Direct from lysate quantitation using signal amplification—no RNA or DNA purification, reverse transcription, or PCR amplification needed
• Precisely quantify subtle differences in gene expression as small as 10%
• Simple workflow features lyse-and-go sample preparation, easily automatable ELISA-like protocol, and readout using any standard luminometer
• Analyze any sample type: cultured cells; whole blood or dried blood spots; fresh, frozen, or formalin-fixed paraffin-embedded (FFPE) tissues; plants; bacteria

QuantiGene Singleplex assays bring the power of branched DNA (bDNA) technology to basic and pre-clinical research. The bDNA assay is a sandwich nucleic acid hybridization method that uses bDNA molecules to amplify signal from captured target RNA. bDNA technology forms the basis of the FDA-approved clinical diagnostic VERSANT 3.0 assays for HIV, HCV, and HBV viral load that are offered commercially by Siemens and have been in use for over a decade. Another advantage of bDNA assays is that RNA is measured directly from the sample source, without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies and variability introduced by or errors inherent to these processes.

Each capture plate in this assay kit is packaged individually in a sealed foil pouch.

Quant-iT™ 1X dsDNA BR Assay Invitrogen™

The Quant-iT 1X dsDNA BR (Broad Range) Assay Kit provides an accurate and precise method for the quantification of dsDNA samples. The assay is highly selective for dsDNA over ssDNA, RNA, and protein. Additionally, this assay tolerates many contaminants common to nucleic acid isolation and purification workflows, such as salts, solvents, detergents, and free nucleotides.

The assay kit is designed to be accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4 ng–4000 ng of DNA.

The '1X' dsDNA BR assay kit provides a ready-to-use working solution. Simply add your sample (any volume between 1 µL and 20 µL), add the Qubit 1X dsDNA BR working solution, and read the concentration using fluorescence-based microplate reader.

Pierce™ Yeast Digest Standard Thermo Scientific™

The Thermo Scientific Pierce Yeast Digest Standard is a lyophilized yeast peptide mixture ideal for monitoring LC/MS system performance.

Pierce Yeast Digest Standard features include:
Positive control sample—well-characterized eukaryotic proteome sample protein digest (>3,000 proteins) for LC/MS method standardization, development, and troubleshooting
Verified peptide quality—digestion conditions optimized for minimal missed cleavages or peptide modifications during processing
Rigorously tested—high-quality, consistent protein digest guaranteed and documented via lot-specific certificates of analysis
Convenient—ready-to-use lyophilized format

The Pierce Yeast Digest Standard is specifically formulated for LC/MS experiments and does not contain salts or detergents. The protein digest is derived from Saccharomyces cerevisiae yeast strain BY4741 (genetic background: MATa his3delta1 leu2delta0 met15delta0 ura3delta0). The yeast proteome is well-documented and characterized, and the digest is an ideal standard for more complex proteome mass spectrometry applications or when a non-mammalian digeste is required for analysis.

Using the standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.

QuantStudio™ 7 Pro Real-Time PCR System, TaqMan™ Array Card Applied Biosystems™

The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System adds innovative smart features to the real-time PCR (qPCR) workflow, all in a compact footprint. With facial authentication, voice commands, Smart Help, multiplex capability, motorized interchangeable blocks, simplified workflow, intuitive software, automation capabilities, and large capacitive touch-screen interface, the QuantStudio 7 Pro system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Designed for ultimate ease-of-use, the system offers high quality, high throughput, excellent reliability, and an optimal user experience for researchers who want to work smarter in the lab.

Features of the QuantStudio 7 Pro Real-Time PCR System include:
• Easy and convenient hands-free operation with facial authentication and voice commands
• Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
• Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
• Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
• Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
• Application versatility with easily changed blocks and four different block types
• Increased productivity with automation compatibility for 24/7 operation
• Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
• Remote monitoring of run status using the Instrument Connect monitoring app
• Design and Analysis software compatible with PC and Mac operating systems
• Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments

Easy and convenient hands-free operation
The QuantStudio 7 Pro system was designed for a simple and powerful qPCR experience. Hands-free operation with facial authentication and voice commands enables users to quickly and easily access their personal profiles and settings without even removing their gloves.

Smart qPCR for ultimate ease of use
With embedded RFID readers, the QuantStudio 7 Pro system has the capability to run TaqMan Array Plates with RFID tags. These RFID tags simplify data tracking (with embedded lot number, expiration date, etc.) and reduce the need for manual data entry with preloaded assay layout, dye information, and thermal protocol. Automating this data transfer allows users to simply load the plate and start the run—no data entry needed.

Learn more about the TaqMan Array Plates with RFID ›

Peace of mind with Smart service
Minimize downtime with premium service offerings for the QuantStudio 7 Pro system. Smart Help allows users to contact Technical Support directly from the touchscreen, without the need to download files or spend time on the phone. Experienced technicians will analyze the request and respond according to the user’s preference (phone or email) and resolve the issue as quickly as possible.

Smart Remote Support is an immersive collaboration tool that allows customers to quickly and efficiently show issues to Technical Support for resolution, helping reduce instrument downtime compared to on-site repair and helping minimize the need for on-site visits.

Connect
The Connect platform provides scientists with a secure place to store, analyze, and share data. Applied Biosystems qPCR Analysis Modules within the platform are web browser-based software tools that are flexible, fast, and easy to use. These software tools support a variety of analyses including relative quantitation, standard curve, high resolution melt (HRM), genotyping, and presence/absence. They are compatible with Mac or PC, as well as most Applied Biosystems instrument file types.

Format flexibility
The QuantStudio 7 Pro system accommodates the interchange of 96-well (0.2 mL), 96-well (0.1 mL), 384-well, and TaqMan array card (TAC) blocks. The system is delivered with one block type; other block types may be purchased separately. In addition, an Orbitor Microplate Mover is available for this system to support high-throughput workflows. For a more value-priced system that does not support TaqMan array cards or automation (but that can be upgraded to do so at a later date), please see our QuantStudio 6 Pro Real-Time PCR System. And for the ultimate in flexibility, consider the QuantStudio 12K Flex Real-Time PCR System, which also accommodates TaqMan OpenArray plates.

Accomplish more, faster
Run hundreds of real-time PCR reactions effortlessly using TaqMan Array 384-well microfluidic cards. And when integrated with the Orbitor Microplate Mover (available separately), the QuantStudio 7 Pro system maximizes your throughput capabilities.

High application versatility
Simple workflows, reliable assays and reagents, multiple formats, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, and gene regulation.

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with the OptiFlex system, which features six decoupled excitation and emission filter channels with 21 filter combinations for maximum multiplexing and chemistry flexibility.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

Qubit™ 1X dsDNA BR Assay Lambda Standards Invitrogen™

The Qubit 1X dsDNA BR (Broad Range) Assay Lambda Standards can be used with either the Qubit 1X dsDNA BR Assay Kit or the Qubit dsDNA BR Assay Kit. Sufficient material is supplied to perform standards with up to 500 samples.

Note: Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex fluorometer.

EVOS™ Light Cube, RFP 2.0 Invitrogen™

Our second-generation EVOS LED light cubes provide superior illumination uniformity across the field of view, resulting in exceptional tile-stitch image quality. At the same time, optimized spectral fidelity across channels significantly improves data quality by minimizing undesired bleed-through. Like the original design, they combine condensing optics and hard-coated filters into one remarkable light engine that delivers digitally controlled fluorophore excitation. These LED light cubes are backwards compatible with older EVOS and Countess systems and can be used with first-generation light cubes. Like the original light cubes, these LED cubes are user-changeable and automatically recognized by the instrument. The EVOS Light Cube, RFP 2.0, is ideal for use with Alexa Fluor 555, Cy3, MitoTracker Orange CMTMRos, pHrodo, RFP, Rhod-2, and other fluorophores with excitation and emission maxima near 542 and 593 nm.

The EVOS Light Cube, RFP 2.0, offers you these important advantages:
• Uniform illumination across the entire field of view for superb image quality
• Optimized spectral compatibility to maximize true signal detection, while minimizing bleed-through for the best possible data quality
• A short light path that affords superior detection of fluorescent signals
• Stable illumination intensity over 50,000 h helps experimental reproducibility

Efficient light engines
Our second-generation light cube design dramatically improves on the original. A larger aperture increases illumination uniformity, which affords superb tile-stitched image quality and consistency. In addition, new LED light and filter combinations maximize the signal-to-background ratio so critical for dim samples, improving data quality irrespective of signal strength for both EVOS and Countess systems. By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Hard-coated filter sets for higher transmission efficiencies
Hard-coated filters are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% higher light transmission compared to soft-coated filters. LED bulbs and filters optimized to maximize the signal from target fluorophores, while minimizing that from other dyes (i.e., bleed-through), will help you obtain brighter fluorescence, higher transmission efficiencies and ability to detect faint fluorescence signals, and better signal-to-noise ratios. These factors raise both image and data qualities.

Continuous light intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. EVOS cubes retain continuous light cube intensity that affords consistent illumination from day-to-day and experiment-to-experiment and help you to directly compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. These digitally controlled light sources make it easy to adjust light intensity, exposure time, and camera gain that can reduce phototoxicity and photobleaching.

Less expensive to own and maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

Explore the entire EVOS line of imaging systems and accessories ›

Pierce™ HeLa Digest/PRTC Standard Thermo Scientific™

The Thermo Scientific Pierce HeLa Digest/PRTC Standard is a validated complex mammalian protein digest containing additional PRTC heavy isotope-labeled peptides designed for LC/MS performance testing.

Features of HeLa Digest/PRTC Standard include:
Convenient—pre-mixed formulation of HeLa digest and PRTC standard in a ready-to-use format
Informative—enables robust performance assessment of LC/MS instruments
Verified—high-quality, consistent mixture documented via lot-specific certificates of analysis
Stable—provided in a lyophilized format

The Pierce HeLa Digest/PRTC Standard is a lyophilized mixture of 10 µg of HeLa protein digest with 5 pmol PRTC peptides to assess chromatography MS instrument performance. The protein digest is derived from a well-established adenocarcinoma reference HeLa S3 cell line that expresses over 15,000 proteins with relevant post-translational modifications. This complex digest is an ideal standard for complex proteome mass spectrometry applications.

The included Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy isotope-labeled peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HFs) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time. In combination, the mixture is a powerful tool to optimize and assess LC parameters, identify the total peptide elution window, and optimize and monitor MS instrumentation.

This standard is specifically formulated for LC/MS experiments and does not contain salts or detergents. Using the standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.

Applications:
• Qualitative LC assessment
• QC assay tool for assessment of LC/MS system performance
• LC/MS standardization
• LC/MS method development and optimization
• Normalize results for variation in retention times and peak intensities between runs

PrepFiler™ 96-Well Processing Plates Applied Biosystems™

The PrepFiler 96-Well Processing Plate is a polypropylene deep-well plate intended for use with the PrepFiler Automated Forensic DNA Extraction Kit or PrepFiler BTA Automated Forensic DNA Extraction Kit. It is used in the purification of sample lysates as part of an automated workflow using robotic liquid handlers.

Both the PrepFiler and PrepFiler BTA Automated Forensic DNA Extraction kits use magnetic particles with an optimized multi-component surface chemistry to deliver superior DNA yield from most forensic sample types. The PrepFiler kit provides superior results with body fluids and stains of body fluids, while the PrepFiler BTA kit was designed specifically for challenging samples such as bones, teeth, and adhesive-based samples (e.g., cigarette butts and tape lifts).

Note: The PrepFiler 96-Well Processing Plate is not compatible with the ID NIMBUS Presto system. See our KingFisher Deepwell 96 Plate, V-bottom, polypropylene (Cat. No. 95040450) for this application.

Qubit™ 1X dsDNA BR Assay Invitrogen™

The Qubit 1X dsDNA BR (Broad Range) Assay kit, designed for use with the Qubit 4 and Qubit Flex fluorometers, provides an accurate and precise method for the quantification of dsDNA samples. The assay is highly selective for dsDNA over ssDNA, RNA, and protein. Additionally, this assay tolerates many contaminants common to nucleic acid isolation and purification workflows, such as salts, solvents, detergents, and free nucleotides.

The assay kit is designed to be accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4–4000 ng of DNA in the assay tube.

The '1X' dsDNA BR assay kit provides a ready-to-use working solution. Simply add your sample (any volume between 1 µL and 20 µL), add the Qubit 1X dsDNA BR working solution, and read the concentration using a Qubit 4 or Qubit Flex fluorometer.

Notes
1. Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex fluorometer.
2. Qubit 4 and Qubit Flex fluorometers may require a firmware update to run the 1X dsDNA BR Assay.

High Resolution Melt (HRM) Plug-in for DA2 Software Applied Biosystems™

High Resolution Melt (HRM) Plug-in for DA2 Software is a tool for High Resolution Melting (HRM) analysis. HRM analysis is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by high-brightness, dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control, advanced data capture capabilities, and access to software designed specifically for HRM analysis. High Resolution Melt (HRM) Plug-in for DA2 Software brings HRM to the QuantStudio 1, QuantStudio 3 and 5, QuantStudio 6 and 7 Pro, StepOne and StepOnePlus Real-Time PCR Systems, and the 7500 Fast Real-Time PCR System, and contains features that make fast and accurate HRM results more accessible than ever.

Key product features:
Minimal user input to achieve results—software automatically applies default/saved analysis settings upon opening an experiment file
Accuracy—clustering algorithm accurately distinguishes control and variant genotypes
Sensitivity—software allows input of expected number of clusters for difficult-to-detect SNPs (class 4)
Ability to achieve more in one run—separate analysis of multiple assays run on a single plate
Shortcuts in experiment setup—direct copy and paste functions with Excel; well information and plate layout clearly displayed in Plate View

Minimize user input to achieve results and easily recall analysis settings
High Resolution Melt (HRM) Plug-in for DA2 Software automatically applies default or saved analysis settings upon opening an experiment file, which allows the user to achieve results with minimal input. It also includes an Analysis Settings Library, allowing the user to save optimized settings and easily recall them for use in future experiments.

Realize accuracy and increase your sensitivity
The software utilizes a clustering algorithm that helps to accurately distinguish control and variant genotypes and allows the user to input the expected number of clusters for increased sensitivity for difficult SNP genotyping.

Achieve more in one run
With High Resolution Melt (HRM) Plug-in for DA2 Software, you have the ability to conduct separate analyses of multiple assays run on a single plate.

Get your experiments up and running fast with shortcuts in setup
Users of High Resolution Melt (HRM) Plug-in for DA2 Software can copy and paste directly from Excel spreadsheets to set up experiments quickly. Plate View also clearly displays well information and the plate layout to ease plate setup.

Download High Resolution Melt (HRM) Plug-in for DA2 Software (license key required) >

Release Notes



PGD-SEQ™ HBB Panel and Reagent Kit Ion Torrent™

The PGD-SEQ HBB (Hemoglobin Subunit Beta) Panel and Reagent Kit provides all the materials necessary to amplify single nucleotide variant (SNV) linkage markers used to resolve the parental mutated and healthy alleles flanking the HBB gene locus in order to research monogenic disease specific to a particular family.

PGD-SEQ kits enable simultaneous detection of informative SNVs surrounding pathogenic variants such as SNVs, insertion/deletions (INDELs), copy number variants (CNVs), and triplet repeat expansion mutations. With dozens of markers, the PGD-SEQ approach reduces the risk of mistyping due to allelic dropout and can be used to classify each sample as affected, carrier, or unaffected. In addition, direct targeting of SNV or INDEL pathogenic variants can be included in the linkage marker assay.

When used with Ion GeneStudio S5 Series or Ion PGM sequencing systems, PGD-SEQ kits enable a simple, end-to-end workflow that includes preimplantation genetic testing for monogenic/single-gene defects (PGT-M) and preimplantation genetic testing for aneuploidies (PGT-A) in the same next-generation sequencing (NGS) run. PGD-SEQ kits are genetic tests specifically designed to overcome allelic drop-out and maximize the identification of an inherited condition by research labs using embryo biopsy samples.

Benefits of the PGD-SEQ HBB Panel and Reagent Kit include:
• PGT-M for HBB using embryo biopsy samples
• Optimized assay kits and software enables PGT-M in most labs
• Improved lab efficiencies in terms of time and expense for PGT

Comprehensive content
The PGD-SEQ kits provide comprehensive coverage of common and rare diseases with >180 demonstrated disease gene panels available. In addition, custom designs can be ordered.

PGD-SEQ kits are an efficient end-to-end solution that includes reagents that employ Ion AmpliSeq technology for target amplification and library preparation and that include software for data analysis. The reagents are optimized to work together out of the box and are ready to use with Ion ReproSeq PGS kits for template preparation and sequencing, enabling rapid combined PGT-M and PGT-A with a results turnaround time suitable for fresh embryo transfers.

Streamlined implementation
The PGT-M workflow begins by ordering the appropriate gene kit for the case. An informativity study is first performed by collecting DNA samples from both parents and additional family members for testing with the PGD-SEQ kit. The resulting embryos from an IVF cycle are biopsied and the samples analyzed using the PGD-SEQ kit and software. Following alignment and variant calling, the PGD-SEQ software is used for SNV phasing analysis to produce the final PGT-M report indicating whether the samples are affected, carrier, or unaffected.

EVOS™ Light Cube, DAPI 2.0 Invitrogen™

Our second-generation EVOS LED light cubes provide superior illumination uniformity across the field of view, resulting in exceptional tile-stitch image quality. At the same time, optimized spectral fidelity across channels significantly improves data quality by minimizing undesired bleed-through. Like the original design, they combine condensing optics and hard-coated filters into one remarkable light engine that delivers digitally controlled fluorophore excitation. These LED light cubes are backwards compatible with older EVOS and Countess systems and can be used with first-generation light cubes. Like the original light cubes, these LED cubes are user-changeable and automatically recognized by the instrument. The EVOS Light Cube, DAPI 2.0, is ideal for use with BFP, DAPI, Hoechst, LysoTracker Blue, NucBlue Live, and other fluorophores with excitation and emission maxima near 357 and 447 nm.

The EVOS Light Cube, DAPI 2.0 offers you these important advantages:
• Uniform illumination across the entire field of view for superb image quality
• Optimized spectral compatibility to maximize true signal detection, while minimizing bleed-through for the best possible data quality
• A short light path that affords superior detection of fluorescent signals
• Stable illumination intensity over 50,000 h helps experimental reproducibility

Efficient light engines
Our second-generation light cube design dramatically improves on the original. A larger aperture increases illumination uniformity, which affords superb tile-stitched image quality and consistency. In addition, new LED light and filter combinations maximize the signal-to-background ratio so critical for dim samples, improving data quality irrespective of signal strength for both EVOS and Countess systems. By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Hard-coated filter sets for higher transmission efficiencies
Hard-coated filters are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% higher light transmission compared to soft-coated filters. LED bulbs and filters optimized to maximize the signal from target fluorophores, while minimizing that from other dyes (i.e., bleed-through), will help you obtain brighter fluorescence, higher transmission efficiencies and ability to detect faint fluorescence signals, and better signal-to-noise ratios. These factors raise both image and data qualities.

Continuous light intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. EVOS cubes retain continuous light cube intensity that affords consistent illumination from day-to-day and experiment-to-experiment and help you to directly compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. These digitally controlled light sources make it easy to adjust light intensity, exposure time, and camera gain that can reduce phototoxicity and photobleaching.

Less expensive to own and maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

Explore the entire EVOS line of imaging systems and accessories ›

EVOS™ Light Cube, Texas Red 2.0 Invitrogen™

Our second-generation EVOS LED light cubes provide superior illumination uniformity across the field of view, resulting in exceptional tile-stitch image quality. At the same time, optimized spectral fidelity across channels significantly improves data quality by minimizing undesired bleed-through. Like the original design, they combine condensing optics and hard-coated filters into one remarkable light engine that delivers digitally controlled fluorophore excitation. These LED light cubes are backwards compatible with older EVOS and Countess systems and can be used with first-generation light cubes. Like the original light cubes, these LED cubes are user-changeable and automatically recognized by the instrument. The EVOS Light Cube, Texas Red 2.0, is ideal for use with Alexa Fluor 594, mCherry, MitoTracker Red CMXRos, mKate, Texas Red, and other fluorophores with excitation and emission maxima near 585 and 628 nm.

The EVOS Light Cube, Texas Red 2.0, offers you these important advantages:
• Uniform illumination across the entire field of view for superb image quality
• Optimized spectral compatibility to maximize true signal detection, while minimizing bleed-through for the best possible data quality
• A short light path that affords superior detection of fluorescent signals
• Stable illumination intensity over 50,000 h helps experimental reproducibility

Efficient light engines
Our second-generation light cube design dramatically improves on the original. A larger aperture increases illumination uniformity, which affords superb tile-stitched image quality and consistency. In addition, new LED light and filter combinations maximize the signal-to-background ratio so critical for dim samples, improving data quality irrespective of signal strength for both EVOS and Countess systems. By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Hard-coated filter sets for higher transmission efficiencies
Hard-coated filters are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% higher light transmission compared to soft-coated filters. LED bulbs and filters optimized to maximize the signal from target fluorophores, while minimizing that from other dyes (i.e., bleed-through), will help you obtain brighter fluorescence, higher transmission efficiencies and ability to detect faint fluorescence signals, and better signal-to-noise ratios. These factors raise both image and data qualities.

Continuous light intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. EVOS cubes retain continuous light cube intensity that affords consistent illumination from day-to-day and experiment-to-experiment and help you to directly compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. These digitally controlled light sources make it easy to adjust light intensity, exposure time, and camera gain that can reduce phototoxicity and photobleaching.

Less expensive to own and maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

Explore the entire EVOS line of imaging systems and accessories ›

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