November 2020 New Products

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Countess™ 3 FL Automated Cell Counter Invitrogen™

The Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting capability, and image analysis software driven by a neural network-based machine-learning algorithm, for rapid assessment of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells, monitor fluorescent protein expression, gain insights to apoptotic processes, and measure cell viability.

Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
Flexible—accepts up to two EVOS light cubes, user selectable from fifteen fluorescent channels
Fast—counts live and dead cells, measures viability, reports average cell size, and saves data with a single touch, in about 20 seconds
Convenient—requires no cleaning or routine maintenance; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training

The Countess 3 FL Automated Cell Counter works in two simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Get accurate cell counts
The Countess 3 FL Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer.

Simple operation with fluorescent application versatility
Operated using an intuitive user interface, automated cell counting with the Countess 3 FL counter gives you the data you need to prepare your sample for the next passage or experiment and allows you to multi-plex with a variety of fluorescent dyes and proteins excited via EVOS light cubes. You no longer need complex, expensive instruments to obtain reliable data.

Transfer data and images to your PC or cloud
Using the USB port on the Countess 3 FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. (For your convenience, a USB drive and WiFI dongle are supplied with the Countess 3 FL instrument).

SARS S1 Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human SARS S1 Ig Total Simplex ProcartaPlex Kit measures anti-SARS S1 antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Countess™ 3 Automated Cell Counter Invitrogen™

The Countess 3 Automated Cell Counter is a benchtop cell counting system equipped with an advanced machine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software for rapid assessment of primary and immortalized cell samples

Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
Fast—counts live and dead cells, measures viability, and reports average cell size with a single touch in about 20 seconds
Convenient—requires no cleaning or routine maintenance; flush-mounted high-resolution capacitive touchscreen and simple user interface provide quick startup, require minimal training, and offer automatic saving of data to USB or cloud

The Countess 3 Automated Cell Counter works in two simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Get accurate cell counts
The Countess 3 Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer.

Transfer data and images to your PC or cloud
Using the USB port on the Countess 3 Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. (For your convenience, a USB drive and WiFI dongle are supplied with the Countess 3 instrument).

Coronavirus Ig Total Human 11-Plex ProcartaPlex™ Panel Invitrogen™

The Human Coronavirus Ig Total 11-Plex ProcartaPlex Panel enables screening of four anti-SARS-CoV-2 antibodies (Spike trimer, S1 subunit, RBD (receptor binding domain), and Nucleocapsid), six anti-coronavirus strains (SARS-CoV-1, MERS, CoV-NL63, CoV-KHU1, CoV-229E, CoV-OC43 ), and one negative control in a single well using Luminex xMAP technology. Simultaneous detection of anti-SARS-CoV-2 antibodies and related coronavirus antibodies in one assay can save time to provide a complete, holistic data set of serum samples.

Unlike most ProcartaPlex kits that utilize matched antibody pairs to detect proteins of interest, this serological assay is designed with specific SARS-CoV-2 proteins conjugated to the bead. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum. IgG is the most abundant of the immunoglobulins.

The panel is provided in a ready-to-use format and the total incubation time is 2.5 hours. The kit comes with two vials each of high, medium, and low controls to be run either as controls or the high control can be serially diluted to generate a standard curve. Serum and plasma samples are recommended to be diluted 1:1000. It is not combinable with simplexes or other panels.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

Target list [bead region]: SARS-CoV-2 Spike (trimer) [14], SARS-CoV-2 S1 protein [15], SARS-CoV-2 RBD (IAP) [75], SARS-CoV-2 Nucleocapsid [13], SARS S1 [67], CoV-NL63 [39], CoV-HKU1 [30], CoV-229E [26], MERS S1 [46], CoV-OC43 [37], Negative control [52]

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

MERS S1 Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human MERS S1 Ig Total Simplex ProcartaPlex Kit measures anti-MERS S1 antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

CoV-NL63 Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human CoV-NL63 Ig Total Simplex ProcartaPlex Kit measures anti-CoV-NL63 antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

SARS-CoV-2 Spike (Trimer) Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human SARS-CoV-2 Spike (trimer) Ig Total Simplex ProcartaPlex Kit measures anti-SARS-CoV-2 Spike (trimer) antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

CoV-OC43 Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human CoV-OC43 Ig Total Simplex ProcartaPlex Kit measures anti-CoV-OC43 antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

CoV-HKU1 Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human CoV-HKU1 Ig Total Simplex ProcartaPlex Kit measures anti-CoV-HKU1 antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

MicroSEQ™ ID Software for SeqStudio™ Genetic Analyzer v1.0, Lite Applied Biosystems™

MicroSEQ ID Software for SeqStudio Genetic Analyzer v1.0 (with additional MicroSEQ libraries) allows you to easily identify and classify unidentified bacterial or fungal sequences by comparing them to a validated microbial library. The Lite version of the software is for use on a stand-alone PC (not connected to the genetic analyzer) for simple analyses.

• Validated library of over 3000 microbial sequences
• Automatically identify unknown specimens
• Run a plate on the SeqStudio Genetic Analyzer directly via the MicroSEQ ID Software
• MicroSEQ system includes security, audit, and e-signature features
• Qualification and validation support available

Confidently identify microbes
The MicroSEQ ID sequence libraries consist of bacterial libraries of 500-bp 16S rDNA sequences and over 2300 validated entries, with extensive coverage of gram-negative non-fermenters, Bacillus, coryneforms, mycobacteria, and Staphylococcus, as well as a fungal library containing more than 1100 entries. To help ensure accurate identification of the species of your sequences, we rigorously validate all library entries. And unlike other widely available databases, MicroSEQ libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms and include bacterial Category A biothreat agents.

Automatically identify unknown specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The Auto-ID feature in MicroSEQ ID Software uses a set of editable parameters to automatically identify unknown specimens. The results table is displayed in the report, and an editable comments field allows additional information or manual identifications to be entered into the same table.

The MicroSEQ system: acomplete, integrated solution
Streamlining every step in microbial identification, the MicroSEQ system combines the advantages of MicroSEQ ID software with easy-to-use PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. The MicroSEQ ID Software also includes security, audit, and electronic signature features, enabling the software to smoothly integrate into your workflow.

Qualification and validation support
To help ensure seamless integration into your laboratory, from installation to routine operation, we offer comprehensive installation and operational qualification (IQ/OQ) and validation support services.

Apoptosis 6-Plex Human ProcartaPlex™ Panel Invitrogen™

The Human Apoptosis 6-Plex ProcartaPlex Panel enables examination of the process of programmed cell death by analyzing 6 protein targets in a single well using Luminex xMAP technology. Simultaneous detection of apoptosis-related proteins along with GAPDH, a housekeeping protein, can help with research of cancer, stroke, Alzheimer's disease, and autoimmune diseases. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit our ProcartaPlex Immunoassays page for more information and available products.

Target list [bead region]: Bcl-2 [57], caspase-3 (active) [44], cleaved PARP [28], cytochrome C [54], GAPDH [12], p53 [52]

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

HyPerforma™ DynaDrive™ Single-Use Bioreactor, 120 V Thermo Scientific™

The Thermo Scientific HyPerforma DynaDrive Single-Use Bioreactor (S.U.B.) drives bioprocessing efficiency and allows users to reimagine bioreactor possibilities. The DynaDrive S.U.B. utilizes a revolutionary stir-tank design to meet cell culture demands of today and the future. The DynaDrive S.U.B. not only offers the advantages of single use, but also provides the mixing and mass transfer performance that demanding cell lines require. The hardware, mixing impeller (drive train), and sparging approach have been redesigned to optimize mixing dynamics, scale, and performance. The DynaDrive technology allows for lower seed-train turndown ratios, which facilitates efficiencies and reduces risk of contamination. The complete line of DynaDrive S.U.B.s include 50, 500, 3000, and 5000 L sizes that ensure consistent scalability from pilot-scale studies to pre-clinical to commercial production. The DynaDrive technology brings single-use to unprecedented volumes and performance.

Features:
• Hardware is jacketed with load cells
• Large volume—50 L, 500 L, and option of scalability to larger sizes of up to 5,000 L
• Improved seed train process—10:1 turndown ratio in the 50 L reactor and 20:1 turndown in the 500 L, plus projected larger-size reactors allow for streamlined seed-train process and help reduce risk
• Mixing times, power input per volume (PIV), and kLa performance are optimized for modern cell culture processes with >100 million cells/mL
• Hardware optimized for perfusion cell culture processes
• Ergonomically friendly hardware—designed with the user in mind and allows for consistent and uniform bioprocess container (BPC) loading
• Open-architecture approach—allows interoperability with available control systems
• HyPerforma DynaDrive BPCs with integrated drive train and made with highly robust Thermo Scientific Aegis 5-14 bioprocessing film
• BPC film and components subjected to leachable and extractable (L&E) evaluation according to BioPhorum Operations Group (BPOG) guidelines

Applications of the Thermo Scientific DynaDrive S.U.B. include:
• Batch, fed batch, and perfusion cultures

CTS™ AIM-V™ Medium, without phenol red, without antibiotics Gibco™

Gibco Cell Therapy Systems (CTS) AIM-V Medium is the first commercially available, defined, serum-free medium (SFM) for the proliferation and/or manipulation of T cells and dendritic cells manufactured in compliance with cGMP. It contains L-glutamine, but phenol red, gentamicin sulfate, and streptomycin sulfate have been removed to meet global regulatory requirements for ancillary materials used in cell therapy manufacturing.

CTS AIM-V Medium can be supplemented with human AB serum or CTS Immune Cell SR and various cytokines or growth factors depending on the application.

• cGMP manufactured in compliance with 21 CFR part 820
• Supports expansion of human peripheral blood T-cells including CAR T cells
• Supports expansion of activated human dendritic cells and other immune cells
• Proven clinical use as an ancillary reagent in Provenge™ (sipuleucel-T), the first therapeutic cancer vaccine approved by the FDA (1)
• Adherence to supplier-related responsibilities of USP <1043>*

Closed systems compatible
For convenience, CTS AIM-V Medium is available in three formats: a 1000-mL bottle for small volume users, a 10-L bag for larger volume users, and a 2-L bioprocess container designed for users interested in a closed system workflow. The BPC chamber is constructed from Aegis 5-14 film and includes sterile, weldable DEPH-free PVC tubing with Luer lock and C-Flex™ tubing with MPC quick connector providing versatile connection options for your manufacturing process.

Security of supply
CTS AIM-V Medium is a defined serum-free formulation and can be manufactured at scale required to meet future clinical and commercial demand. Alternate manufacturing sites are available as further risk mitigation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform to cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485-certified. Gibco CTS products follow USP <1043> 'ancillary materials for cell, gene, and tissue-engineered products' within the responsibilities applicable to a supplier.*

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

Immune cell applications
CTS AIM-V Medium is cited in many peer-reviewed journals and used in multiple immune cell clinical applications such as activated dendritic cells. It supports expansion of T cells, T cell subsets, and virus-specific T cells. The medium can also be used to support natural killer cells combined with feeders. Each lot of CTS AIM-V SFM is pre-qualified using Jurkat cells, an immortalized human T lymphocyte line, to help ensure consistent performance.

*Other aspects of USP <1043> will be the responsibility of the end-user to assess. Thermo Fisher Scientific cannot fulfill USP <1043> in regards to application and therapy-specific aspects (e.g., residual assessment and removal of the AM).

1. Madan RA, Gulley JL. Sipuleucel-T: harbinger of a new age of therapeutics for prostate cancer. Expert Rev Vaccines. 2011 10(2):141-50.

CaptureSelect™ FcXP Ligand Leakage ELISA Thermo Scientific™

The CaptureSelect FcXP Ligand Leakage ELISA detects possible FcXP affinity ligand contamination of the purified product after use of CaptureSelect FcXP Affinity Matrix.

The FcXP affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix for high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This sensitive leakage ELISA detects affinity ligand contamination of less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect FcXP Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

SARS-CoV-2 S1 Protein Ig Total Human ProcartaPlex™ Simplex Kit Invitrogen™

The Human SARS-CoV-2 S1 Protein Ig Total Simplex ProcartaPlex Kit measures anti-SARS-CoV-2 S1 protein antibodies in serum and plasma. The detector antibody is an Immunoglobulin (Ig) Total antibody directly conjugated to phycoerythrin (PE) that cross-reacts with all Ig (IgG, IgM, and IgA) in serum and plasma. IgG is the most abundant of the immunoglobulins.

This kit is designed to be combinable with other Simplex kits from the Coronavirus Panel so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one Viral Reagent kit (sold separately) is needed for each assay plate regardless of plex size.

About ProcartaPlex assays for the Luminex platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

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