January 2021 New Products

xMAP™ Sheath Concentrate PLUS, RUO Invitrogen™

xMAP Sheath Concentrate PLUS, RUO, is the delivery medium that carries the sample to the optics component of Luminex xMAP technology-based instruments. It is needed to operate all Luminex 200, FLEXMAP 3D, xMAP INTELLIFLEX, and xMAP INTELLIFLEX DRSE systems.

xMAP Sheath Concentrate PLUS, RUO, is conveniently packaged as a concentrated fluid (20X) that is diluted before use. It is also available at a 1X concentration (Cat. No. 4050021).

This is a reformulation of xMAP Sheath Fluid Concentrate (Cat. No. 4050018). The formulation is updated from a single preservative to a mixture of three preservatives to allow for greater transit stability and to reduce the likelihood of future acquired microbial resistance.

Please refer to a Luminex instrument user manual for proper use of xMAP Sheath Concentrate PLUS, RUO, with particular Luminex instruments.

POROS™ CaptureSelect™ AAVX EvolveD™ Column Thermo Scientific™

POROS CaptureSelect AAVX EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for purification of AAV viral vectors used for gene therapy applications and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Regulatory Support File available upon request
• Packed with POROS CaptureSelect AAVX Affinity Matrix, offering high specificity and capacity over a wide range of flow rates

Operating and technical specifications
Column type5-cm bed height10-cm bed height
Column diameter (cm)710710
Column length (cm)551010
Cross-sectional area (cm2)38.578.538.578.5
Column volume (mL)190390385785

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm
• Inlet/outlet port connections: 25-mm TC sanitary fitting

Features of POROS CaptureSelect AAVX resin include:
• One-step AAV purification from crude material with high purity and yield
• High specificity and capacity, maximizing yield by significantly reducing process steps
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• High capacity maintained at high flow rates
• Excellent scalability and easier handling and packing due to rigid polymeric bead
• Non-animal derived—the ligand is a 13-kDa single-domain fragment, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components

POROS CaptureSelect AAVX Affinity Resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step. It has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8, AAVrh10, and synthetic serotypes.

Main characteristics:

Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect AAVX affinity ligand
Binding capacity: >1x1013 genome copies/mL of resin
Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Formulation buffer: 18% (v/v) ethanol

Axiom™ Multispecies Shrimp and Tilapia Genotyping Array (Axiom™ TilShrv1), mini-96 format

The Axiom Multispecies Shrimp and Tilapia Genotyping Array (Axiom TilShrv1) was designed in collaboration with João Campos and Jose Fernando Garcia from Trigene, Brazil, with financial support from PIPE-FAPESP. The array contains 60,886 SNPs from tilapia and 6,453 SNPs from shrimp. It is also available in 384HT format.

Tilapia content
• Contains 60,886 SNPs from tilapia
• Twelve different strains of Nile tilapia from wild African samples to farmed GIFT, Chitralada, Stirling, and other strains obtained from different origins in Brazil and North America, representing most of the variability of Nile tilapia in the world
• A total of 16 million variants identified after mapping reads from 158 samples to the Nile tilapia reference genome (GCF001858045.2 O. niloticus UMD-NMBU)
• Post-filtering, more than 2 million bi-allelic SNPs investigated for inclusion on the array
• Highly polymorphic SNPs with the no adjacent polymorphisms were selected
• Final set of SNPs had an average distance of 14 kbp on the 22 linkage groups and high allele frequency, with only a few having >5% SNPs with a minor allele frequency lower than 0.05

Applications for tilapia array
• Genome wide association studies
• Molecular breeding—association mapping and genomic selection
• Population genetics—distinguish among fish origin
• Distinguish between different strains of Nile tilapia

Shrimp content
• Contains 6,453 SNPs from shrimp
• Markers selected within the L. vannamei genome from a dataset of over 350,00 putative variants (1,2)
• Evenly spaced markers

Applications for shrimp array
• Genomic selection and evaluation for family identification within Pacific white shrimp (Litopenaeus vannamei)

References
1. Du ZQ, Ciobanu DC, Onteru SK, Gorbach D, Mileham AJ, Jaramillo G, Rothschild MF. A gene-based SNP linkage map for pacific white shrimp, Litopenaeus vannamei. Anim Genet. 2010 Jun;41(3):286-94.
2. Jones DB, Jerry DR, Khatkar MS, Raadsma HW, Steen HV, Prochaska J, Forêt S, Zenger KR. A comparative integrated gene-based linkage and locus ordering by linkage disequilibrium map for the Pacific white shrimp, Litopenaeus vannamei. Sci Rep. 2017 Sep 4;7(1):10360.

Required products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software
Axiom Long Format Export Tool (AxLE)

Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) Invitrogen™

Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) enables fast and efficient enrichment of intact, live SARS-CoV-2 coronavirus from dilute cell culture and virus transport media. The enrichment is gentle and fast and can be performed in less than three hours. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The isolated intact virus can be used for functional studies, in contrast to current SARS-CoV-2 purification methods based on extracting RNA for detection purposes. Intact Virus Precipitation Reagent allows for enrichment of intact virus from large, dilute samples for further virus growth or use in any downstream functional assays, protein research, PCR, etc.

Why use Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)
Simple—3-step SARS-CoV-2 enrichment requiring only a centrifuge
Fast—enrichment in <3 hours with minimal hands-on time
Flexible volume—dilute virus samples can be enriched from large sample volumes
Straight to PCR—no further nucleic acid extraction is needed prior to PCR

How to use Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)
Use twice as much virus sample as precipitation reagent (e.g., use 2 mL virus sample per 1 mL reagent).

1. Add precipitation reagent to the virus sample and incubate for 2 hours at 2–8°C.
2. Centrifuge for 30 minutes at 10,000 x g.
3. Remove supernatant and keep the pellet with the enriched virus.

The enriched virus is now ready for any downstream analysis and can be used directly in a PCR reaction without any further nucleic acid extraction.

For higher throughput automation needs for intact SARS-CoV-2, use the magnetic bead-based Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2) in combination with one of our KingFisher instruments.

The Covid-19 pandemic has led to an explosion of SARS-CoV-2 detection with PCR and sequencing. But SARS-CoV-2 research has a growing need to understand how the virus works, how it influences the body and immune system, how the virus evolves over time, and how to develop new vaccines and therapies. This increases the need to study the intact virus. In addition, virus-like particles (VLPs), containing only virus-specific proteins and not nucleic acids, are extensively used in viral proteomic research. Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) isolates both live and inactivated SARS-CoV-2 intact virus and VLPs. The reagent can also be used on other viruses.

If you are in a process of customizing Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) in a commercial product or service, or you need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com.

Dynabeads™ Intact Virus Enrichment (optimized for SARS-CoV-2) Invitrogen™

Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2) are 1-µm strong anion exchange magnetic beads that bind negatively charged vesicles and molecules. The beads have been tested and optimized for enrichment of intact, live SARS-CoV-2 coronavirus from dilute cell culture and virus transport media. The enrichment is gentle and fast and can be performed in as little as 20 minutes. The virus can be easily removed from the beads in a 10-minute release step. The isolated intact virus can be used for functional studies, in contrast with current SARS-CoV-2 purification methods based on extracting RNA for detection purposes. The enriched intact virus can be used for further virus growth or in any downstream functional assays, protein research, purification of RNA for PCR, etc. In addition, the Dynabeads magnetic beads allow for high-throughput isolation in combination with any of our KingFisher instruments, thus both manual and automated protocols are available.

Why use Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2)
Fast isolation—intact SARS-CoV-2 virus in ~20 minutes
Simple automation—just push the button and walk away
High throughput—enrich up to 96 samples per run
Functional virus—the intact virus can be used in any functional assay
Release—the virus can be released from the beads in ~10 minutes

How to use Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2) (manual protocol)
A mixer/roller and a DynaMag magnet is required for the washing steps. Mix the beads thoroughly before starting. Use ~20 µL Dynabeads/mL virus sample.

1. Wash the beads twice using the magnet.
2. Add beads to sample for 10 minutes at room temperature.
3. Wash the bead-bound virus twice on the magnet to remove unbound target.
4. Optional: Release the virus from the beads in ~10 minutes

The enriched virus is now ready for any downstream analysis.

The automated protocol is even simpler
1. Add the reagents to the plates, load the plates into the instrument and push the button.
2. Come back in 20 minutes to pick up the enriched virus (max 96 samples/run).

The COVID-19 pandemic has led to an explosion of SARS-CoV-2 detection with PCR and sequencing. But SARS-CoV-2 research has a growing need to understand how the virus works, how it influences the body and immune system, how the virus evolves over time, and how to develop new vaccines and therapies. Thus, there is an increasing need to study the intact virus. In addition, virus-like particles (VLPs), containing only virus-specific proteins and not nucleic acids, are extensively used in viral proteomic research. Dynabeads Intact Virus Enrichment Beads (optimized for SARS-CoV-2) isolate both live and inactivated SARS-CoV-2 intact virus and VLPs. The beads can also be used on other enveloped viruses, exosomes, and any other negatively charged vesicles and molecules.

In addition, with use of a KingFisher instrument, the automated protocols can help increase the consistency of the results as well as save hands-on time and total time if working with many samples simultaneously.

If you are in a process of customizing Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2) in a commercial product or service, or you need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com.

TrueMark™ Respiratory Panel 2.0, OpenArray™ plate Applied Biosystems™

The TrueMark Respiratory Panel 2.0, OpenArray plate, is an efficient, easy-to-use fixed-content OpenArray plate for the characterization of key respiratory tract microbial targets. The plate includes 35 TaqMan assays that have been optimized for detection of 32 respiratory tract viral and bacterial microbes, including two assays that target SARS-CoV-2.

The plate also includes control assays for TaqMan Universal Extraction Control Organism (B. atrophaeus), TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control, and the human RNase P RPPH1 gene. For a complete list of assays included in the plate see the TrueMark Respiratory Panel 2.0, OpenArray plate, product information sheet.

The microbial assays and the Xeno control are plated in triplicate, and the B. atrophaeus and RPPH1 assays are plated in duplicate.

Features of this syndromic panel-based real-time PCR solution include:
Extensive coverage—featuring a higher number of respiratory pathogenic organism targets than similar competitor products
Cost-effective workflow—lowest cost per sample when compared with other commercially available molecular solutions
Multiple sample input types—including nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasopharyngeal aspirate
Highest throughput—run up to 24 samples per plate

Each TrueMark Respiratory Panel 2.0, OpenArray plate, utilizes a microscope slide–sized stainless steel plate with 3,072 wells for individual 33-nL reactions where TaqMan assays are spotted. Simply set up the AccuFill system to transfer your reactions to the OpenArray plate, seal the plate, then load it into the instrument for results.

TaqMan assay designs and assay target sequences have undergone rigorous bioinformatics selection and analysis to maximize strain coverage and minimize potential for off‐target cross-reactivity. Qualified TaqMan assays also undergo performance testing to ensure that results are accurate and reproducible with high levels of analytical sensitivity and specificity.

The TrueMark Respiratory Panel 2.0, OpenArray plate, is orderable as single plates and is intended for use with the AccuFill and QuantStudio 12K Flex systems.

TrueMark™ Respiratory Panel 2.0 Amplification Control Applied Biosystems™

The TrueMark Respiratory Panel 2.0 Amplification Control contains a linearized multi-target plasmid with target sequences for each respiratory tract microbiota assay on the TrueMark Respiratory Panel 2.0, OpenArray plate and TrueMark Respiratory Panel 2.0, TaqMan array card. The control can be included in respiratory tract microbiota profiling experiments as a positive control for panel-specific amplification.

The plasmid also contains target sequences for TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control, TaqMan Universal Extraction Control Organism (B. atrophaeus), and the human RNase P RPPH1 gene. In addition, the plasmid contains target sequences for additional qualified TaqMan assays for respiratory tract microbiota profiling that may be included on custom arrays or cards. Refer to the Respiratory Tract Microbiota Profiling Experiments v2 using TaqMan Array Cards Application Guide or Respiratory Tract Microbiota Profiling Experiments v2 using OpenArray Application Guide for the full assay list.

The TrueMark Respiratory Panel 2.0 Amplification Control is supplied at a concentration of 1 x 105 copies/μL. During real-time PCR, the control can be used as a stand-alone sample to verify assay performance and to help with troubleshooting.

TrueMark™ Respiratory Panel 2.0, TaqMan™ Array card Applied Biosystems™

The TrueMark Respiratory Panel 2.0, TaqMan Array card, is an efficient, easy-to-use TaqMan Array card for the characterization of key respiratory tract microbes. The array card includes 44 TaqMan assays that have been optimized for detection of 41 respiratory tract viral, bacterial, and fungal microbes, including two assays that target SARS-CoV-2.

The array card also includes control assays for TaqMan Universal Extraction Control Organism (B. atrophaeus), TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control, the human RNase P RPPH1 gene, and the human 18S ribosomal RNA gene. For a complete list of assays included in the array card see the TrueMark Respiratory Panel 2.0, TaqMan Array card, product information sheet.

Features of this syndromic panel-based real-time PCR solution include:
Extensive coverage—featuring the largest number of markers of pathogenic organisms of any test on the market
Cost-effective workflow—lowest cost per sample when compared with other commercially available molecular solutions
Multiple sample input types—including nasopharyngeal (NP) swabs, bronchoalveolar lavage (BAL), and nasopharyngeal aspirate
Optimal throughput—run up to 8 samples per array card

Each TrueMark Respiratory Panel 2.0, TaqMan Array card, contains a select group of TaqMan microbial assays pre-configured in a 384-well micro-fluidic array card (48-assay format). Simply add the pre-amplified product to each port along with our TaqMan Fast Advanced Master Mix, no UNG, and nuclease-free water, centrifuge and seal the card, then load it into the instrument for results.

TaqMan assay designs and assay target sequences have undergone rigorous bioinformatics selection and analysis to maximize strain coverage and minimize potential for off‐target cross-reactivity. Qualified TaqMan assays also undergo performance testing to ensure that results are accurate and reproducible with high levels of analytical sensitivity and specificity.

The TrueMark Respiratory Panel 2.0, TaqMan Array card, is orderable as single cards and is intended for use with the QuantStudio 7 and QuantStudio 12K Flex systems with a TaqMan Array Card block.

MagMAX™ Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit Applied Biosystems™

The MagMAX Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit is designed for research use. The kit recovers RNA and DNA from virus and gram-negative bacteria in samples such as blood, swabs, urine, and transport media. MagMAX magnetic bead technology produces reproducible recovery of high-quality nucleic acids compatible with a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.

Features of the MagMAX Viral/Pathogen II (MVP II) Nucleic Acid Isolation Kit include:
• Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems
• Fast procedure allows for 96 samples to be processed in <30 minutes
• Flexible protocol accommodates sample volume inputs from 200 µL to 2 mL of biofluids or transport media
• No need for carrier RNA
• Elution volumes ranging from 50 to 100 µL

Maximum sample input in a 96-deep well or 24-deep well plate format
The optimized reagents included in the MagMAX Viral/Pathogen II (MVP II) kit allow you to maximize the amount of sample input, thereby increasing the amount of RNA and/or DNA recovered. Up to 400 µL of sample can be processed per well in a KingFisher 96-deep well plate. For larger sample volumes, protocols are available to process up to 2 mL of sample per well in a KingFisher 24-deep well plate.

Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid. Beads bind the nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields. Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with protein-rich, large-volume samples such as whole blood or plasma that are commonly used for viral testing.

Axiom™ Multispecies Shrimp and Tilapia Genotyping Array (Axiom™ TilShrv1), 384HT format

The Axiom Multispecies Shrimp and Tilapia Genotyping Array (Axiom TilShrv1) was designed in collaboration with João Campos and Jose Fernando Garcia from Trigene, Brazil, with financial support from PIPE-FAPESP. The array contains 60,886 SNPs from tilapia and 6,453 SNPs from shrimp. It is also available in mini-96 format.

Tilapia content
• Contains 60,886 SNPs from tilapia
• Twelve different strains of Nile tilapia from wild African samples to farmed GIFT, Chitralada, Stirling, and other strains obtained from different origins in Brazil and North America, representing most of the variability of Nile tilapia in the world
• A total of 16 million variants identified after mapping reads from 158 samples to the Nile tilapia reference genome (GCF001858045.2 O. niloticus UMD-NMBU)
• Post-filtering, more than 2 million bi-allelic SNPs investigated for inclusion on the array
• Highly polymorphic SNPs with the no adjacent polymorphisms were selected
• Final set of SNPs had an average distance of 14 kbp on the 22 linkage groups and high allele frequency, with only a few having >5% SNPs with a minor allele frequency lower than 0.05

Applications for tilapia array
• Genome wide association studies
• Molecular breeding—association mapping and genomic selection
• Population genetics—distinguish among fish origin
• Distinguish between different strains of Nile tilapia

Shrimp content
• Contains 6,453 SNPs from shrimp
• Markers selected within the L. vannamei genome from a dataset of over 350,00 putative variants (1,2)
• Evenly spaced markers

Applications for shrimp array
• Genomic selection and evaluation for family identification within Pacific white shrimp (Litopenaeus vannamei)

References
1. Du ZQ, Ciobanu DC, Onteru SK, Gorbach D, Mileham AJ, Jaramillo G, Rothschild MF. A gene-based SNP linkage map for pacific white shrimp, Litopenaeus vannamei. Anim Genet. 2010 Jun;41(3):286-94.
2. Jones DB, Jerry DR, Khatkar MS, Raadsma HW, Steen HV, Prochaska J, Forêt S, Zenger KR. A comparative integrated gene-based linkage and locus ordering by linkage disequilibrium map for the Pacific white shrimp, Litopenaeus vannamei. Sci Rep. 2017 Sep 4;7(1):10360.

Required products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software
Axiom Long Format Export Tool (AxLE)

TrueMark™ Respiratory Panel 2.0 PreAmp Primers Applied Biosystems™

The TrueMark Respiratory Panel 2.0 PreAmp Primers kit contains primers for all respiratory tract microbiota assays plus the TaqMan Universal RNA Spike In/Reverse Transcription (Xeno) Control and the RNase P RPPH1 gene, but does not contain primers for the TaqMan Universal Extraction Control Organism (B. atrophaeus).

The primers are intended to be used in the preamplification step of the TrueMark Respiratory Panel 2.0, OpenArray plate and TrueMark Respiratory Panel 2.0, TaqMan array card workflows to help achieve the highest analytical sensitivity with the added benefit of sample conservation.

AccuCheck™ ERF Reference Particles Invitrogen™

AccuCheck ERF Reference Particles are used for standardization and calibration of an intensity unit of fluorescence for flow cytometry analysis. Through the application of the assigned NIST-traceable intensity standard for a given fluorophore, instrument sensitivity and accuracy can be determined, permitting data comparison between flow cytometry instruments. In combination with a biological standard, these particles can also be used to quantify the level of biomarker expression.

AccuCheck ERF Reference Particles features include:
• Lot-specific NIST-traceable ERF values assigned to 26 channels
• Enables fluorescence intensity measurements for instrument accuracy and sensitivity
• Facilitates instrument to instrument data comparison

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