February 2021 New Products

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QuantStudio™ 5 Dx Real-Time PCR System, desktop Applied Biosystems™

The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is an in vitro diagnostic (IVD) quantitative PCR system that delivers proven performance and support to help meet the needs of clinical/hospital laboratories and test developers, in a compact footprint and cost-effective package.

Flexibility and security
The QuantStudio 5 Dx software is a combined-function software that enables both Development (DEV) and In Vitro Diagnostic (IVD) operation as discrete operating modes, minimizing risk of operation in the incorrect instance.

The software enables you to maintain secure access to the instrument, specify user access to various software functions, record all actions completed by users, and sign data electronically by virtue of Security, Auditing, and E-signature features.

This QuantStudio 5 Dx system is offered in a 96-well, 0.2 mL configuration and accommodates reaction volumes from 10 to 100 uL, as well as both standard and fast chemistries to meet varying user demands.

Trusted performance
Resulting from over 20 years of real-time PCR experience, the QuantStudio 5 Dx system includes proven Applied Biosystems OptiFlex technology and VeriFlex blocks for enhanced data quality. By enabling de-coupling of excitation and emission filters with OptiFlex technology, users may optimize performance for specific dyes and when multiplexing. With VeriFlex blocks, users may precisely set the temperature on six independently-controlled thermal zones across the 96-well sample block for optimal PCR amplification performance.

Cost effective
The QuantStudio 5 Dx system is our most affordable IVD qPCR platform. It is factory-calibrated and, following installation qualification and operational qualification by a factory-certified engineer, ready for operation by trained users. Subsequent IVD dye calibrations are now user-enabled for convenience and to minimize ongoing operating costs.

Amplitude™ High‐Throughput Consumable Package 2 Assay Applied Biosystems™

The Amplitude High‐Throughput Consumable Package 2 Assay consists of assays and controls for 20,000 real-time RT-PCR tests for the detection of SARS-COV-2 RNA in nasal and nasopharyngeal swab samples. Laboratories can run up to 8,000 tests in 24 hours using the Amplitude Solution, and the consumable package format enables efficient ordering to address high-throughput needs.

This package is part of Cat. No. A50883 and is to be used in conjunction with Amplitude High‐Throughput Consumable Package 1 Reagents.

The ordering of Amplitude Solution reagents and consumables is managed by your sales representative and requires a laboratory supply agreement.

HyPerforma™ DynaDrive™ Single-Use Bioreactor, 240 V Thermo Scientific™

The Thermo Scientific HyPerforma DynaDrive Single-Use Bioreactor (S.U.B.) drives bioprocessing efficiency and allows users to reimagine bioreactor possibilities. The DynaDrive S.U.B. utilizes a revolutionary stir-tank design to meet cell culture demands of today and the future. The DynaDrive S.U.B. not only offers the advantages of single use, but also provides the mixing and mass transfer performance that demanding cell lines require. The hardware, mixing impeller (drive train), and sparging approach have been redesigned to optimize mixing dynamics, scale, and performance. The DynaDrive technology allows for lower seed-train turndown ratios, which facilitates efficiencies and reduces risk of contamination. The complete line of DynaDrive S.U.B.s include 50, 500, 3000, and 5000 L sizes that ensure consistent scalability from pilot-scale studies to pre-clinical to commercial production. The DynaDrive technology brings single-use to unprecedented volumes and performance.

Features:
• Hardware is jacketed with load cells
• Optimal-precision load cells
• Large volume—50 L, 500 L, 3000 L, and 5,000 L; working volume options available
• Improved seed train process
   --50 L: 10:1 turndown ratio; seed with as little as 5 L
   --500 L: 20:1 turndown ratio; seed with as little as 25 L
   --3000 L: 12.5:1 turndown ratio; seed with as little as 250 L
   --5000 L: 20:1 turndown ratio; seed with as little as 250 L
• Mixing times, power input per volume (PIV), and kLa performance are optimized for modern cell culture processes with >100 million cells/mL
• Hardware optimized for perfusion cell culture processes
• Ergonomically friendly hardware—designed with the user in mind and allows for consistent and uniform bioprocess container (BPC) loading
   --All BPC connections done at ground level and BPC deployed automatically with a lift mechanism
• Open-architecture approach—allows interoperability with available control systems
• HyPerforma DynaDrive BPCs with integrated drive train and made with highly robust Thermo Scientific Aegis 5-14 bioprocessing film
• BPC film and components subjected to leachable and extractable (L&E) evaluation according to BioPhorum Operations Group (BPOG) guidelines

Applications of the Thermo Scientific DynaDrive S.U.B. include:
• Batch, fed batch, and perfusion cultures

TaqMan™ SARS-CoV-2 Control Applied Biosystems™

The Applied Biosystems TaqMan SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays that are included in the TaqMan SARS-CoV-2 Pooling Assay (Cat. No. A50790). The control should be diluted in TaqMan SARS-CoV-2 Control Dilution Buffer (Cat. No. A49889).

Amplitude™ High‐Throughput Consumable Package 1 Reagents Applied Biosystems™

The Amplitude High‐Throughput Consumable Package 1 Reagents contains reagents for 20,000 real-time RT-PCR tests for the detection of SARS-COV-2 RNA from nasal and nasopharyngeal swab samples. Laboratories can run up to 8,000 tests in 24 hours using the Amplitude Solution, and the consumable package format enables efficient ordering to address high-throughput needs.

This package is part of Cat. No. A50884 and is to be used in conjunction with the Amplitude High‐Throughput Consumable Package 2 Assay.

The ordering of Amplitude Solution reagents and consumables is managed by your sales representative and requires a laboratory supply agreement.

Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible Thermo Scientific

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:
• Chelator stable—stable in buffer containing 20 mM EDTA and DTT
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
• High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.

TaqMan™ SARS-CoV-2, FluA, FluB RNA Control Applied Biosystems

The TaqMan SARS-CoV-2, Flu A, Flu B RNA Control contains targets specific to the SARS-CoV-2, influenza A, and influenza B genomic regions targeted by the TaqMan SARS-CoV-2, FluA, FluB RT-PCR Assay Kit. It is for use with this assay as a positive control to ensure reaction setup and reagent integrity when interpreting test results.

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina™ Systems, with UD indexes (Set A-D, 1-96) Invitrogen™

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

TaqMan™ SCID/SMA Assay Applied Biosystems™

The Applied Biosystems TaqMan SCID/SMA Assay is a multiplex real-time PCR assay for simultaneous screening of severe combined immunodeficiency (SCID) and spinal muscular atrophy (SMA) directly from dried blood spot (DBS) samples. It detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control. For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Assay detects SMN1, TREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

The TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects exon 7 of SMN1 and TREC. The assay includes the RNase P gene as an internal amplification control, verifying successful sample extraction. The assay is optimized for high specificity to SMN1, eliminating cross-detection of SMN2.

Viral-Plex™ Complexation Buffer, prototype Gibco™

Gibco Viral-Plex Complexation Buffer, prototype, is a chemically defined, protein-free, phenol red-free buffer for complexing plasmid DNA with transfection reagent for standard transfection applications.

HyPerforma™ DynaDrive™ BioProcess Container Thermo Scientific™

This bioprocess container (BPC) for the HyPerforma DynaDrive Single-Use Bioreactor (S.U.B.) is made with our proprietary Aegis5-14 film. This film is widely used by many global biopharmaceutical companies for all bioprocessing steps. Aegis5-14 film was introduced in 2013 and has a clean profile, with completed BPOG leachable and extraction (L&E) testing guidelines for all components used in HyPerforma DynaDrive BPCs.

Key benefits
We offer both standard and custom BPC configurations. Standard configurations are for a variety of common applications, while custom configurations are for customer-specific applications. The key benefits for our BPCs include:
  • Enhanced sparging technology to maximize efficiency while delivering improved mass transfer performance
  • The latest sensor technology in single-use or autoclavable probes, including:
    • pH—electrochemical single-use or reusable probes, and optical single-use sensor
    • pO2—optical single-use or reusable probes
    • Foam—single-use foam sensor built into BPC
    • Capacitance—single-use sensor or reusable probe
    • Raman—optical probe port into BPC
  • Optional ports for alternating tangential flow (ATF) or tangential flow filtration (TFF)
  • Built-in condenser system for 3,000 and 5,000 L BPCs, which helps extend the life and reduce the quantity of exhaust filters needed
  • Utilization of crossflow sparging technology to assure proper head-space gas mixing during low-volume seed or terminal cell cultures
  • Integrated drive-train mixing technology, utilizing four impellers in the 5,000 L DynaDrive S.U.B. and two impellers in the 3,000 L DynaDrive S.U.B. Users no longer need to load a drive shaft into the BPC, thus reducing time and ceiling height constraints

TaqMan™ SCID/SMA Plus Assay Applied Biosystems™

The Applied Biosystems TaqMan SCID/SMA Plus Assay is a multiplex real-time PCR assay for simultaneous screening of severe combined immunodeficiency (SCID) and spinal muscular atrophy (SMA) directly from dried blood spot (DBS) samples. It detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control. For an assay that detects only SMN1 and TREC, please see the TaqMAN SCID/SMA Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Plus Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Plus Assay detects SMN1, TREC, and KREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from 96- to 384-well plates is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

TaqMan™ SARS-CoV-2, Flu A/B, RSV RNA Control Applied Biosystems

The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit. It is recommended for use with this assay as a positive control to ensure reaction setup and reagent integrity when interpreting test results.

AAV-MAX Transfection Kit, prototype Gibco™

The Gibco AAV-MAX Transfection Kit, prototype, is an essential component of the AAV-MAX Helper-Free AAV production system. It is formulated for the transient transfection of Viral Production Cells 2.0 (Cat. No. A50232). The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium (Cat. No. A49842DK).

TaqPath™ COVID-19, FluA, FluB Combo Kit Applied Biosystems

The TaqPath COVID-19, Flu A, Flu B Combo Kit includes the assays and controls for a multiplex real-time RT-PCR test for the qualitative detection and differentiation of RNA from SARS-CoV-2, influenza A, and influenza B viruses in nasopharyngeal and anterior nasal swabs from individuals suspected of COVID-19 by their healthcare provider.

Features of the TaqPath COVID-19, Flu A, Flu B Combo Kit include:
Single test for SARS-CoV-2, influenza A, and influenza B—detect and differentiate between disease states with similar clinical symptoms and get information needed to help monitor spread of COVID-19 and flu
Affordable and scalable—add to your existing COVID-19 testing menu and infrastructure to expand your respiratory sample testing while maintaining low operational costs and workflow simplicity
Multitarget assay design—helps compensate for emerging SARS-CoV-2 variants and mutations to help provide confidence in results
Complete solution for clinical labs—Applied Biosystems Pathogen Interpretive Software automatically converts genetic analysis data into a readable report, helping reduce risk of user interpretation error
Emergency Use Authorized

The TaqPath COVID-19, Flu A, Flu B Combo Kit includes the following components:
TaqPath RT-PCR COVID-19, Flu A, Flu B Kit
   --TaqPath COVID-19, Flu A, Flu B RT-PCR Assay Multiplex—multiplexed assays that contain primer and probe sets specific to two SARS-CoV-2 targets (S gene and N gene), one influenza A target, one influenza B target, and bacteriophage MS2
   --MS2 Phage Control—internal process control for nucleic acid extraction
TaqPath COVID-19, Flu A, Flu B Control—RNA control that contains targets specific to the SARS- CoV-2, influenza A, and influenza B genomic regions targeted by the assays
TaqPath Control Dilution Buffer

The TaqPath COVID-19, Flu A, Flu B Combo Kit is authorized for use on the Applied Biosystems 7500 Fast Dx Real-Time PCR and QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL block) with nasopharyngeal swab and anterior nasal swab specimens only.

Access the Instructions for Use approved for your country › 

Intended use
The TaqPath COVID-19, FluA, FluB Combo Kit is a multiplex real-time RT-PCR test intended for the simultaneous qualitative detection and differentiation of RNA from the SARS-CoV-2, influenza A, and/or influenza B viruses in nasopharyngeal swab and anterior nasal swab specimens collected from individuals suspected of respiratory viral infection consistent with COVID-19 by their healthcare provider. Clinical signs and symptoms of respiratory viral infection due to SARS-CoV-2 and influenza can be similar. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, that meet the requirements to perform high-complexity tests.

The TaqPath COVID-19, FluA, FluB Combo Kit is intended for use in simultaneous detection and differentiation of SARS-CoV-2, influenza A, and/or influenza B nucleic acid in clinical specimens and is not intended to detect influenza C virus. The SARS-CoV-2, influenza A, and influenza B RNA is generally detectable in upper respiratory samples during the acute phase of infection.

Positive results are indicative of active infection but do not rule out bacterial infection or co-infection with other pathogens not detected by the test. Clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. The agent detected may not be the definite cause of disease.

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