February 2021 New Products

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Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible Thermo Scientific

Pierce high-capacity EDTA-compatible Ni-IMAC resin is designed for the purification of over-expressed His-tagged proteins from cell culture media. The matrix is a 6% crosslinked agarose with optimal properties for batch, gravity column, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC resin include:
• Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
• Reusable—can be regenerated up to 10 times using the CIP protocol

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping off the metal. Because of its chelator stability characteristic (EDTA tolerant), Pierce high-capacity EDTA-compatible Ni-IMAC resin is especially suited for purifying expressed His-tagged proteins that are secreted into cell culture media or for purifying intracellular His-tagged proteins that need the presence of EDTA to maintain stability and function. In both cases, large quantities of samples can be purified without the Ni metal stripping off of the support, thereby providing consistent high capacity yields.

TaqMan™ SARS-CoV-2, FluA, FluB RT-PCR Assay Kit Applied Biosystems

The TaqMan SARS-CoV-2, Flu A, Flu B assay is a multiplex real-time RT-PCR assay for the detection of RNA from the SARS-CoV-2 virus, the influenza A virus, and the influenza B virus.

Features of the TaqMan SARS-CoV-2, FluA, FluB, RT-PCR Assay Kit include:
Single assay for SARS-CoV-2, influenza A, and influenza B RNA—detect and differentiate between infection states and get the information needed to help analyze distribution of SARS-CoV-2 and flu nucleic acid
Affordable and scalable—add to your existing SARS-CoV-2 assay menu and infrastructure to expand your respiratory sample analysis while maintaining low operational costs and workflow simplicity
Sensitive and specific—robust performance for detecting SARS-CoV-2 and flu A and B strains, providing increased confidence in results

Other reagents recommended for use with the TaqMan SARS-CoV-2, FluA, FluB, RT-PCR Assay Kit include:
TaqMan SARS-CoV-2, Flu A, Flu B RNA Control
TaqMan SARS-CoV-2 Control Dilution Buffer
TaqPath 1-Step RT-qPCR Master Mix

Collibri™ ES DNA Library Prep Kit for Illumina™ Systems, with UD indexes (Set A-D, 1-96) Invitrogen™

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo eassembly

TaqMan™ SARS-CoV-2, Flu A/B, RSV RT-PCR Assay Kit Applied Biosystems

Detect RNA from multiple respiratory viruses simultaneously with the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit, a highly accurate, real-time PCR multiplex assay that allows you to expand your respiratory sample analysis menu while maintaining low operational costs.

Features of the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit include:
Single assay for SARS-CoV-2, influenza A/B, and RSV RNA—detect and differentiate between infection states and get the information needed to help analyze distribution of SARS-CoV-2, flu, and RSV nucleic acid
Affordable and scalable—add to your existing SARS-COV-2 assay menu and infrastructure to expand your respiratory sample analysis while maintaining low operational costs and workflow simplicity
Sensitive and specific—get 100% homology to >99.9% of known SARS‐CoV‐2 genomes* and robust performance for detecting flu A/B and RSV RNA, providing increased confidence in results
Complete solution for labs—Applied Biosystems Design & Analysis Software automatically converts genetic analysis data into a readable report, helping reduce risk of user interpretation error

Other reagents recommended to be used in conjunction with the TaqMan SARS-CoV-2, FluA/B, RSV RT-PCR Assay Kit include:
TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control
TaqMan SARS-CoV-2 Control Dilution Buffer
TaqPath 1-Step RT-qPCR Master Mix

TaqMan™ SCID/SMA Assay Applied Biosystems™

The Applied Biosystems TaqMan SCID/SMA Assay is a multiplex real-time PCR assay for simultaneous screening of severe combined immunodeficiency (SCID) and spinal muscular atrophy (SMA) directly from dried blood spot (DBS) samples. It detects the SMN1 gene and TREC, as well as the RNase P gene as an internal genomic control. For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Assay detects SMN1, TREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

The TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects exon 7 of SMN1 and TREC. The assay includes the RNase P gene as an internal amplification control, verifying successful sample extraction. The assay is optimized for high specificity to SMN1, eliminating cross-detection of SMN2.

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina™ Systems, with UD indexes (Set A-D, 1-96) Invitrogen™

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

HyPerforma™ DynaDrive™ Single-Use Bioreactor, 240 V Thermo Scientific™

The Thermo Scientific HyPerforma DynaDrive Single-Use Bioreactor (S.U.B.) drives bioprocessing efficiency and allows users to reimagine bioreactor possibilities. The DynaDrive S.U.B. utilizes a revolutionary stir-tank design to meet cell culture demands of today and the future. The DynaDrive S.U.B. not only offers the advantages of single use, but also provides the mixing and mass transfer performance that demanding cell lines require. The hardware, mixing impeller (drive train), and sparging approach have been redesigned to optimize mixing dynamics, scale, and performance. The DynaDrive technology allows for lower seed-train turndown ratios, which facilitates efficiencies and reduces risk of contamination. The complete line of DynaDrive S.U.B.s include 50, 500, 3000, and 5000 L sizes that ensure consistent scalability from pilot-scale studies to pre-clinical to commercial production. The DynaDrive technology brings single-use to unprecedented volumes and performance.

Features:
• Hardware is jacketed with load cells
• Optimal-precision load cells
• Large volume—50 L, 500 L, 3000 L, and 5,000 L; working volume options available
• Improved seed train process
   --50 L: 10:1 turndown ratio; seed with as little as 5 L
   --500 L: 20:1 turndown ratio; seed with as little as 25 L
   --3000 L: 12.5:1 turndown ratio; seed with as little as 250 L
   --5000 L: 20:1 turndown ratio; seed with as little as 250 L
• Mixing times, power input per volume (PIV), and kLa performance are optimized for modern cell culture processes with >100 million cells/mL
• Hardware optimized for perfusion cell culture processes
• Ergonomically friendly hardware—designed with the user in mind and allows for consistent and uniform bioprocess container (BPC) loading
   --All BPC connections done at ground level and BPC deployed automatically with a lift mechanism
• Open-architecture approach—allows interoperability with available control systems
• HyPerforma DynaDrive BPCs with integrated drive train and made with highly robust Thermo Scientific Aegis 5-14 bioprocessing film
• BPC film and components subjected to leachable and extractable (L&E) evaluation according to BioPhorum Operations Group (BPOG) guidelines

Applications of the Thermo Scientific DynaDrive S.U.B. include:
• Batch, fed batch, and perfusion cultures

Viral-Plex™ Complexation Buffer, prototype Gibco™

Gibco Viral-Plex Complexation Buffer, prototype, is a chemically defined, protein-free, phenol red-free buffer for complexing plasmid DNA with transfection reagent for standard transfection applications.

TaqMan™ SARS-CoV-2 Control Applied Biosystems™

The Applied Biosystems TaqMan SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays that are included in the TaqMan SARS-CoV-2 Pooling Assay (Cat. No. A50790). The control should be diluted in TaqMan SARS-CoV-2 Control Dilution Buffer (Cat. No. A49889).

TaqMan™ SCID/SMA Plus Assay Applied Biosystems™

The Applied Biosystems TaqMan SCID/SMA Plus Assay is a multiplex real-time PCR assay for simultaneous screening of severe combined immunodeficiency (SCID) and spinal muscular atrophy (SMA) directly from dried blood spot (DBS) samples. It detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control. For an assay that detects only SMN1 and TREC, please see the TaqMAN SCID/SMA Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Plus Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Plus Assay detects SMN1, TREC, and KREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from 96- to 384-well plates is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

Viral Production Medium, prototype Gibco™

Gibco Viral Production Medium, prototype, is a chemically defined, serum-free, protein-free medium specially developed for growth and transfection of Viral Production Cells 2.0 (suspension-adapted HEK 293F-derived cells) (Cat. No. A50532) for adeno-associated virus (AAV) production as part of the Gibco AAV-MAX Helper-Free AAV production system.

Viral Production Medium, prototype, requires supplementation with 4 mM GlutaMAX Supplement (Cat. No. 35050038) prior to use.

Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible Thermo Scientific

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are designed for purification of over-expressed His-tagged proteins from cell culture media. The beads have high binding capacity (up to 80 mg of protein per mL of settled beads) and can tolerate the high EDTA and reducing agent concentrations that are used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC MagBeads include:
• Chelator stable—stable in buffer containing 20 mM EDTA and DTT
• Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
• Exceptional high protein binding capacity—up to 80 mg/mL of settled beads
• High-performance—non-aggregating, superparamagnetic microparticles provide exceptional performance with manual and automated HTS applications (e.g., Thermo Scientific KingFisher instruments)

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads consist of crosslinked agarose beads embedded with magnetite. The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added benefits of having magnetic properties and being EDTA compatible. The MagBeads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications.

Pierce high-capacity EDTA-compatible Ni-IMAC MagBeads are optimized for use with high-throughput magnetic platforms, such as the KingFisher 96 and KingFisher Flex magnetic particle processors, but the beads also compatible with simple benchtop purification applications using an appropriate magnetic stand. Automated instruments, however, are especially useful for higher throughput purification and screening of purification conditions.

AAV-MAX Transfection Kit, prototype Gibco™

The Gibco AAV-MAX Transfection Kit, prototype, is an essential component of the AAV-MAX Helper-Free AAV production system. It is formulated for the transient transfection of Viral Production Cells 2.0 (Cat. No. A50232). The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium (Cat. No. A49842DK).

TaqMan™ SARS-CoV-2 Control Dilution Buffer Applied Biosystems™

Applied Biosystems TaqMan SARS-CoV-2 Control Dilution Buffer is used to dilute the TaqMan SARS-CoV-2 RNA Control (Cat. No. 956118), which is used with the TaqMan SARS-CoV-2 Pooling Assay Kit (Cat. No. A50790). This Dilution Buffer is also used to dilute the TaqMan SARS-CoV-2, Flu A, Flu B RNA Control and TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control, which are used with the TaqMan SARS-CoV-2, Flu A, Flu B RT-PCR Assay Kit (Cat. No. A47701) and TaqMan SARS-CoV-2, Flu A/B, RSV RT-PCR Assay Kit (Cat. No. A47702).

TaqMan™ SARS-CoV-2, FluA, FluB RNA Control Applied Biosystems

The TaqMan SARS-CoV-2, Flu A, Flu B RNA Control contains targets specific to the SARS-CoV-2, influenza A, and influenza B genomic regions targeted by the TaqMan SARS-CoV-2, FluA, FluB RT-PCR Assay Kit. It is for use with this assay as a positive control to ensure reaction setup and reagent integrity when interpreting test results.
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