December 2018 New Products

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, desktop (Applied Biosystems™)

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Download Quantstudio 1 Design & Analysis Software >   |   Release notes

Features of the QuantStudio 1 system include:
Touchscreen—a large 8.5-inch interactive touchscreen
Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
Reaction volume—10–100 μL
Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
Protocols—pre-optimized protocol templates
Software—intuitive software that supports analysis via web browser or desktop computer
Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions
Dimensions (H x W x D) and weight—40 x 27 x 50 cm, <26 kg

Thermo Fisher Connect
Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use
The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through Thermo Fisher Connect
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 and Windows® 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

MagMAX™ Total RNA Elution Buffer (Applied Biosystems™)

This elution buffer is included in the MagMAX-96 Total RNA Isolation Kit (Cat. No. AM1830) and MagMAX-96 Viral RNA Isolation Kit (AM1836). It is made available here for separate purchase.

Atherosclerosis 9-plex Human ProcartaPlex™ Panel 1 (Invitrogen™)

The Human Atherosclerosis 9-plex ProcartaPlex Panel 1 is a preconfigured multiplex immunoassay kit that measures 9 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: tenascin C, HSP 60, LOX-1, MPO, FGF-2, PAI-1, TRAIL, IL-8, IL-6
Bead regions: tenascin C: 28; HSP 60: 13; LOX-1: 76; MPO: 18; FGF-2: 75; PAI-1: 35; TRAIL: 66; IL-8: 27; IL-6: 25
Assay sensitivity (as determined for Lot 1): tenascin C: 119.2 pg/mL; HSP 60: 107.1 pg/mL; LOX-1: 0.3 pg/mL; MPO: 7.8 pg/mL; FGF-2: 4.5 pg/mL; PAI-1: 3.0 pg/mL; TRAIL: 0.6 pg/mL; IL-8: 0.2 pg/mL; IL-6: 1.5 pg/mL

Lp-PLA2 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Lp-PLA2 Simplex ProcartaPlex kit measures Lp-PLA2 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types:cell culture supernatant, serum, plasma
Sample volume:serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

alamarBlue™ HS Cell Viability Reagent (Invitrogen™)

alamarBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. High background fluorescence caused by various contaminants reduces the performance of resazurin-based reagents. To improve the performance, an innovative purification process was developed that removes these contaminants. This highly purified, non-toxic resazurin was used in the standard alamarBlue formulation, creating the multiple-component alamarBlue HS Cell Viability Reagent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. The alamarBlue HS Cell Viability Reagent has broad applicability and can be used with various human and animal cell lines, bacteria, plant, and fungi.

alamarBlue HS Cell Viability Reagent features include:
Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
Signal-to-background ratio increased by >100%—results in large assay signal window
Highly sensitive reagent with a linear response—detects as few as 20 cells per well
Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
Measures viability from many diverse cell types—including mammalian cells, bacteria, plant, and fungi

Measuring changes in cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. Cell health can be monitored by detecting changes in several key indicators, including changes to plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing environment.

Monitoring changes to the cellular reducing environment or metabolic activity by using resazurin-based reagents is a well-established and reliable indicator of cell viability or death. Upon entering living cells, the cellular reducing environment reduces resazurin to resorufin, a compound that is red in color and highly fluorescent. Viable cells continuously convert resazurin to resorufin, increasing the overall fluorescence of the medium surrounding the cells. Additionally, the conversion of resazurin to resorufin results in a pronounced color change, thus cell viability can also be detected using absorbance-based plate readers.

As a consequence of the synthesis and manufacturing processes, all resazurin-based reagents contain a detectable amount of resorufin contamination. The amount of contamination can vary greatly between sources of the material and manufacturing conditions, contributing to differences in detectable background fluorescence. More importantly, the contaminating resorufin reduces the signal to background ratio and dynamic range of the assay. An innovative process was developed that removes the contaminating resorufin, resulting in the highly pure resazurin used in alamarBlue HS Cell Viability Reagent.

alamarBlue HS Cell Viability Reagent is a complete add-and-read, non-toxic reagent that does not require cell lysis. The highly purified resazurin used for alamarBlue HS reagent results in a >50% decrease in background fluorescence and a >100% increase in the signal-to-background ratio. Since no lysis is required, the diluted alamarBlue HS solution can be removed and replaced with complete growth medium for further culturing of the cells. Similar to alamarBlue reagent, alamarBlue HS reagent displays an extended viability detection time window for a wide variety of organisms and thus can be used with various human and animal cell lines, bacteria, plant, and fungi, resulting in quantitative measurement of cell viability and proliferation.

PrestoBlue™ HS Cell Viability Reagent (Invitrogen™)

PrestoBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. High background fluorescence caused by various contaminants reduces the performance of resazurin-based reagents. To improve the performance an innovative purification process was developed that removes these contaminants. This highly purified, non-toxic resazurin was used in the standard PrestoBlue formulation, creating the PrestoBlue HS Cell Viability Reagent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. The PrestoBlue HS Cell Viability Reagent has been optimized for the fast and reliable detection of mammalian cell viability.

PrestoBlue HS Cell Viability Reagent features include:
• Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
• Signal-to-background ratio increased by >100%—results in large assay signal window
• Highly sensitive reagent with a linear response—detects as few as 10 cells per well
• Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
• Optimized for reliable detection of mammalian cell viability in only 10 minutes

Measuring changes in cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. Cell health can be monitored by detecting changes in several key indicators, including changes to plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and cellular reducing environment.

Monitoring changes to the cellular reducing environment or metabolic activity by using resazurin-based reagents is a well-established and reliable indicator of cell viability or death. Upon entering living cells, the cellular reducing environment reduces resazurin to resorufin, a compound that is red in color and highly fluorescent. Viable cells continuously convert resazurin to resorufin, increasing the overall fluorescence of the medium surrounding the cells. Additionally, the conversion of resazurin to resorufin results in a pronounced color change, thus cell viability can also be detected using absorbance-based plate readers.

As a consequence of the synthesis and manufacturing processes, all resazurin-based reagents contain a detectable amount of resorufin contamination. The amount of contamination can vary greatly between sources of the material and manufacturing conditions, contributing to differences in detectable background fluorescence. An innovative process was developed that removes the contaminating resorufin, resulting in the highly pure resazurin used in PrestoBlue HS Cell Viability Reagent. This highly pure resazurin produces a >50% decrease in background fluorescence and a >100% increase in the signal-to-background ratio. Mammalian cell viability can be detected after a 10-minute incubation with PrestoBlue HS Cell Viability Reagent. Since no lysis is required, the diluted PrestoBlue HS solution can be removed and replaced with complete growth medium, for further culturing of the cells.

CaptureSelect™ KappaXP Affinity Matrix (Thermo Scientific™)

CaptureSelect KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's. It enables efficient elution, even at pH 6.0, and demonstrates an improved stability for caustic cleaning agents. It is specific for human antibodies or antibody fragments, without cross-specificity to bovine antibody.

CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all kappa-containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.

CaptureSelect KappaXP Affinity Matrix features include:
• Purification of kappa monoclonal antibodies, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Non–animal-derived

The high selectivity and high yields obtained using CaptureSelect KappaXP Affinity Matrix enable a robust and efficient purification process with excellent purity in one step.

View other antibody therapeutic affinity matrix formats ›

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single domain antibody fragments. The KappaXP ligand is a 13-kDa single-domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide immobilization of the ligand
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop (Applied Biosystems™)

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Download Quantstudio 1 Design & Analysis Software >   |   Release notes

Features of the QuantStudio 1 system include:
Touchscreen—a large 8.5-inch interactive touchscreen
Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
Reaction volume—10–100 μL
Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
Protocols—pre-optimized protocol templates
Software—intuitive software that supports analysis via web browser or desktop computer
Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions
Dimensions (H x W x D) and weight—40 x 27 x 50 cm, <26 kg

Thermo Fisher Connect
Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use
The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through Thermo Fisher Connect
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 and Windows® 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

HSP60 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human HSP60 Simplex ProcartaPlex kit measures HSP60 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types:cell culture supernatant, serum, plasma
Sample volume:serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL (Applied Biosystems™)

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Download Quantstudio 1 Design & Analysis Software >   |   Release notes

Features of the QuantStudio 1 system include:
Touchscreen—a large 8.5-inch interactive touchscreen
Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
Reaction volume—10–100 μL
Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
Protocols—pre-optimized protocol templates
Software—intuitive software that supports analysis via web browser or desktop computer
Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions
Dimensions (H x W x D) and weight—40 x 27 x 50 cm, <26 kg

Thermo Fisher Connect
Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use
The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through Thermo Fisher Connect
• No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows® 7 and Windows® 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase

Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

CHI3L1/YKL-40 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CHI3L1/YKL-40 Simplex ProcartaPlex kit measuresCHI3L1/YKL-40 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types:cell culture supernatant, serum, plasma
Sample volume:serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

Atherosclerosis 4-plex Human ProcartaPlex™ Panel 2 (Invitrogen™)

The Human Atherosclerosis 4-plex ProcartaPlex Panel 2 is a preconfigured multiplex immunoassay kit that measures 4 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: Lp-PLA2, CHI3L1/YKL-40, ICAM-1, adiponectin
Bead regions: Lp-PLA2: 19; CHI3L1/YKL-40: 34; ICAM-1: 73; adiponectin: 57
Assay sensitivity (as determined for Lot 1): Lp-PLA2: 1.67 pg/mL; CHI3L1/YKL-40: 13.7 pg/mL; ICAM-1: 74.4 pg/mL; adiponectin: 35.3 pg/mL

Oncomine Focus Assay, Chef-Ready Library (Ion Torrent™)

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. It provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single workflow. For a kit that includes reagents for manual library preparation, please see the Oncomine Focus Assay, AmpliSeq Library.

The Oncomine Focus Assay, Chef-Ready Library, is for the Ion Chef System and Ion GeneStudio S5 System workflow. It contains all the reagents needed to prepare libraries for 32 samples:

Oncomine Focus Assay, DNA Chef-Ready panel—contains the DNA primer pool
Oncomine Focus Assay, RNA Chef-Ready panel—contains the RNA primer pool
Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System

From sample to answer
The Oncomine Focus Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.Learn more about Oncomine Focus Assays and Oncomine Oncology.

Tenascin-C Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Tenascin-C Simplex ProcartaPlex kit measures tenascin-C protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types:cell culture supernatant, serum, plasma
Sample volume:serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

Choline Chloride (Gibco™)

Choline chloride is an essential nutrient commonly found in eggs, chicken, and number of other foods.

Applications: choline chloride is a standard component of cell culture media that serves as a phospholipid precursor for the cells.

Mechanism of action: choline serves as a precursor component to two major cell membrane components: phosphatidylcholine and sphingomyelin. In addition, choline functions as a methyl group donor to several different substrates.

Gibco Chemicals is a line of raw materials that fulfill needs throughout the drug development process to commercial production. Standard features include cGMP manufacturing, custom specifications, and a variety of package sizes, as well as a portfolio of cGMP supply chain services to meet every requirement.

Benefits of Gibco Chemicals include:
• Access the materials you need for your R&D and manufacturing processes
• Realize manufacturing on a global scale with multi-compendial classifications
• Maximize supply chain visibility with transparency of manufacturing and distribution
• Eliminate need for re-validation with comprehensive technical documentation
• Streamline supply chain operations by outsourcing key needs such as safety stock storage, custom labeling, and poly-palletization

A focus on quality
Gibco Chemicals are manufactured and distributed through ISO-certified, cGMP-compliant facilities to meet your regulatory needs and mitigate risk.

Transparency of supply chain
Technical documentation, sales support, and technical service teams are available to provide full visibility into product specifications and the journey from the manufacturer to your hands.

Custom needs are a standard offering
Gibco Chemicals can be customized to meet your unique specifications, including custom fill and weigh requirements, testing protocols, and tailgate samples.