Dynabeads IMS Reagents & BeadRetriever Magnetic Bead Processor

Dynabeads™ anti-Salmonella (Applied Biosystems™)

For the selective enrichment of Salmonella directly from pre-enrichment samples using ImmunoMagnetic Separation. Method can be performed manually or can be automated by using a BeadRetriever™. Performance tested: Meets the requirements of AOAC RI License no.970401

Benefits and features

• Increases Sensitivity and positive isolation rate
• Easy IMS Protocol - 30 minutes from start to finish
• Shelf stable reagent and low start up costs

This Product Contains: Dynabeads® coated with anti-Salmonella antibodies

Sensitivity:
Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre-enrichment.

Dynabeads™ MAX E. coli O157 Kit (Applied Biosystems™)

Dynabeads® MAX E.Coli O157 provides a powerful sample preparation method to improve PCR detection and quantification of E.Coli O157:H7 in food, feces, water and other environmental samples.
Based on well known and widely used immunomagnetic separation (IMS) technology, Dynabeads® MAX E.Coli O157 provides a selective E.Coli O157:H7 capture step that delivers significant improvements in PCR analytical process time, as well as the sensitivity and consistency of results. With Dynabeads® MAX E.Coli O157, you'll:

• Perform high throughput, rapid, and effective E.Coli O157:H7 sample preparation
• Significantly shorten sample pre-enrichment time
• Improve detection sensitivity in PCR sample analysis
• Significantly reduce overall analysis time
• Can be carried out manually or automated using the BeadRetriever™ System

Dynabeads™ EPEC/VTEC O145 (Applied Biosystems™)

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever™.

This Product Contains: Dynabeads® coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ anti-E. coli O157 (Applied Biosystems™)

Dynabeads® E. coli anti-O157 is a tool for better sample preparation and improved cell culture and immuno-based assays for E. coli O157:H7 in food, feces, water and other environmental samples. Based on well-known and widely used immunomagnetic separation (IMS) technology, Dynabeads® E. coli anti-O157 provides a useful means to selectively capture E. coli O157:H7 and better prepare samples for effective analysis.

With Dynabeads® anti-E. coli O157, you will:

• Isolate and concentrate E. coli O157:H7 from samples in as little as 24 hours
• Improve detection sensitivity in cell culture and immunoassays of samples
• Perform either manual or automated (using the BeadRetriever™ System) separations

Dynabeads™ MAX EPEC/VTEC O121 Kit (Applied Biosystems™)

The Dynabeads® MAX EPEC/VTEC O121 Kit allows for the rapid, selective concentration of E. coli O121 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the BeadRetriever™ System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the BeadRetriever™ System.

Sensitivity
Following the Dynabeads® MAX EPEC/VTEC O121 protocol, the presence or absence of one viable E. coli O121 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

NOTE: Lot 1375851 of this product contains only 1 vial of paramagnetic beads (1 mL each) sufficient for 100 reactions. This batch follows a slightly modified protocol (see use instructions in document part number MAN0007304 Revision 1.0).

Dynabeads™ MAX EPEC/VTEC O45 Kit (Applied Biosystems™)

The Dynabeads® MAX EPEC/VTEC O45 Kit allows for the rapid, selective concentration of E. coli O45 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the BeadRetriever™ System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the BeadRetriever™ System.

Sensitivity
Following the Dynabeads® MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

Dynabeads™ Rotary Mixer Mixing Platform (Applied Biosystems™)

When attached to the Dynabeads® Rotary Mixer (Cat. No. 947-01), the Mixing Platform can hold Dynabeads in 3 MPC®-S racks or one 96-well microcentrifuge tube holder provided with the Mixing Platform. Variable speed (15-80 rpm). Can be operated in both cold and warm conditions (4-37°C).

Dynabeads™ MPC™-S (Magnetic Particle Concentrator) (Applied Biosystems™)

Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml.

Note that this magnet is for microbiology applications only, not for eukaryotic cell separations. For use in food and environmental testing only. Not for any animal or human therapeutic or diagnostic use.

Dynabeads™ EPEC/VTEC O111 (Applied Biosystems™)

For the rapid selective concentration of E.coli 0111. Method can be performed manually or can be automated by using a BeadRetriever™.

This Product Contains: Dynabeads® coated with anti-E.coli 0111 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ Rotary Mixer (Applied Biosystems™)

A rotisserie-style mixer providing continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids). Inter-changeable rod accommodates different tube sizes and sample volumes. Basic rod designed for 14 X 1.5 ml tubes or 12 X 10-15 ml tubes. Additional rod (Cat. No. 947-02) available for 14 X 30-50 ml tubes.

Dynabeads™ EPEC/VTEC O26 (Applied Biosystems™)

For the rapid selective concentration of E.coli 026. Method can be performed manually or can be automated by using a BeadRetriever™.

This Product Contains: Dynabeads® coated with anti-E.coli O26 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

BeadRetriever™ Tubes & Tips (Applied Biosystems™)

The tube strips are designed for processing and isolation of target molecules; one sample uses one tube strip which is made up of five tubes. The disposable plastic tips are designed for shielding the magnetic surface from contact with the sample. The BeadRetriever™ System utilize low binding, disposable plastics materials for optimal magnetic particle processing.

Dynabeads™ MPC™-1 (Magnetic Particle Concentrator) (Applied Biosystems™)

The MPC®-1 (Magnetic Particle Concentrator) holds one tube of variable diameter (10–30 mm, 5–50 mL tube). Optimal working volume: 5–50 mL. Magnetic particle concentrators are designed to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids).

Note that this magnet is for microbiology applications only, not for cell separations.

Dynabeads™ MX 8-tube Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold 8 sample tubes ranging from 15 to 27 mm in diameter. Requires use of Mixer Turret (Cat. No. 159-06) to affix to Mixer Base.

Dynabeads™ Spot-On™ Slides (Applied Biosystems™)

Treated, single 9 mm well, glass slides for microscopic analysis of oocysts and cysts. This product is used in combination with the Dynabeads® anti-Cryptosporidium and GC-Combo kits.