Dynabeads IMS Reagents & BeadRetriever Magnetic Bead Processor

Dynabeads™ EPEC/VTEC O26 (Applied Biosystems™)

For the rapid selective concentration of E.coli 026. Method can be performed manually or can be automated by using a BeadRetriever™.

This Product Contains: Dynabeads® coated with anti-E.coli O26 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ MX 12-tube Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold 12 sample tubes ranging from 1.5 ml (microcentrifuge) to 1.7 mm diameter. Requires use of Mixer Turret (Cat. No. 159-06) to affix to Mixer Base.

Dynabeads™ MX 8-tube Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold 8 sample tubes ranging from 15 to 27 mm in diameter. Requires use of Mixer Turret (Cat. No. 159-06) to affix to Mixer Base.

Dynabeads™ Rotary Mixer (Applied Biosystems™)

A rotisserie-style mixer providing continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids). Inter-changeable rod accommodates different tube sizes and sample volumes. Basic rod designed for 14 X 1.5 ml tubes or 12 X 10-15 ml tubes. Additional rod (Cat. No. 947-02) available for 14 X 30-50 ml tubes.

Dynabeads™ MPC™-S (Magnetic Particle Concentrator) (Applied Biosystems™)

Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml.

Note that this magnet is for microbiology applications only, not for eukaryotic cell separations. For use in food and environmental testing only. Not for any animal or human therapeutic or diagnostic use.

Dynabeads™ MX MPC™-6 Semi-automated Mixer Head (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold one MPC®-6 (Cat. No. 120-02D) sample rack; capacity up to 6 tubes.

Dynabeads™ Spot-On™ Slides (Applied Biosystems™)

Treated, single 9 mm well, glass slides for microscopic analysis of oocysts and cysts. This product is used in combination with the Dynabeads® anti-Cryptosporidium and GC-Combo kits.

Dynabeads™ EPEC/VTEC O145 (Applied Biosystems™)

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever™.

This Product Contains: Dynabeads® coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

BeadRetriever™ System (Applied Biosystems™)

Based on magnetic separation technology, the BeadRetriever™ System enables rapid purification and concentration of selected targets from a broad range of samples. Partnered with Dynabeads® immunomagnetic separation (IMS) products, the BeadRetriever™ system provides an optimized solution for the detection of microorganisms of importance in the analysis of food, water, and other environmental samples.

o Magnetic Dynabeads® particle-based microorganism purification
o Protocols included for Cryptosporidium, E. Coli 0157, EPEC/VTEC, Giardia, Legionella, Listeria and Salmonella.
o Automated processing of up to 15 samples in ~40 min
o High reproducibility and maximum performance of capture technology
o Simple, easy operation-complete processing through internal program
o Small footprint-uses only 11.8 in (30 cm) of bench depth

Dynabeads™ Rotary Mixer Rod (Applied Biosystems™)

A mixing rod designed for use with the Dynabeads® Rotary Mixer (Cat. No. 947-01). Holds 14 X 30-50 ml tubes.

Dynabeads™ MAX EPEC/VTEC O121 Kit (Applied Biosystems™)

The Dynabeads® MAX EPEC/VTEC O121 Kit allows for the rapid, selective concentration of E. coli O121 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the BeadRetriever™ System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the BeadRetriever™ System.

Sensitivity
Following the Dynabeads® MAX EPEC/VTEC O121 protocol, the presence or absence of one viable E. coli O121 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

NOTE: Lot 1375851 of this product contains only 1 vial of paramagnetic beads (1 mL each) sufficient for 100 reactions. This batch follows a slightly modified protocol (see use instructions in document part number MAN0007304 Revision 1.0).

Dynabeads™ MX2 Mixer (Applied Biosystems™)

The Dynabeads® MX2 Mixer combines Dynabeads® MX Mixer Base (Cat. No. 159-02), Mixer Turret (Cat. No. 159-06), and 8-tube Mixing Wheel (Cat. No. 159-04). Designed for sample tubes ranging from 15 to 27 mm in diameter.

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, nucleic acids). Variable speed (5-40 rpm). Can be operated in both cold and warm conditions (4-37°C). Inter-changeable heads accommodate different tube sizes and sample volumes.

BeadRetriever™ Tubes & Tips (Applied Biosystems™)

The tube strips are designed for processing and isolation of target molecules; one sample uses one tube strip which is made up of five tubes. The disposable plastic tips are designed for shielding the magnetic surface from contact with the sample. The BeadRetriever™ System utilize low binding, disposable plastics materials for optimal magnetic particle processing.

Dynabeads™ Rotary Mixer Mixing Platform (Applied Biosystems™)

When attached to the Dynabeads® Rotary Mixer (Cat. No. 947-01), the Mixing Platform can hold Dynabeads in 3 MPC®-S racks or one 96-well microcentrifuge tube holder provided with the Mixing Platform. Variable speed (15-80 rpm). Can be operated in both cold and warm conditions (4-37°C).

Dynabeads™ MX4 Mixer (Applied Biosystems™)

The Dynabeads® MX4 Mixer combines Dynabeads® MX Mixer Base (Cat. No. 159-02) and MPC®-S Mixing Head (Cat. No. 159-11). Designed to hold two MPC®-S sample racks (Cat. No. A13346); capacity up to 12 microcentrifuge tubes.

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, nucleic acids). Variable speed (5-40 rpm). Can be operated in both cold and warm conditions (4-37°C). Inter-changeable heads accommodate different tube sizes and sample volumes.