Dynabeads IMS Reagents & KingFisher mL Food Protection Purification System

Dynabeads™ EPEC/VTEC O111 (Applied Biosystems™)

For the rapid selective concentration of E.coli 0111. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

This Product Contains: Dynabeads coated with anti-E.coli 0111 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ anti-Salmonella (Applied Biosystems™)

For the selective enrichment of Salmonella directly from pre-enrichment samples using ImmunoMagnetic Separation. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System;.

Benefits and features

• Increases Sensitivity and positive isolation rate
• Easy IMS Protocol - 30 minutes from start to finish
• Shelf stable reagent and low start up costs

This Product Contains: Dynabeads coated with anti-Salmonella antibodies

Sensitivity:
Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre-enrichment.

Dynabeads™ MAX E. coli O157 Kit (Applied Biosystems™)

Dynabeads MAX E.Coli O157 provides a powerful sample preparation method to improve PCR detection and quantification of E.Coli O157:H7 in food, feces, water and other environmental samples.
Based on well known and widely used immunomagnetic separation (IMS) technology, Dynabeads MAX E.Coli O157 provides a selective E.Coli O157:H7 capture step that delivers significant improvements in PCR analytical process time, as well as the sensitivity and consistency of results. With Dynabeads MAX E.Coli O157, you'll:

• Perform high throughput, rapid, and effective E.Coli O157:H7 sample preparation
• Significantly shorten sample pre-enrichment time
• Improve detection sensitivity in PCR sample analysis
• Significantly reduce overall analysis time
• Can be carried out manually or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Dynabeads™ MPC™-1 (Magnetic Particle Concentrator) (Applied Biosystems™)

The MPC®-1 (Magnetic Particle Concentrator) holds one tube of variable diameter (10–30 mm, 5–50 mL tube). Optimal working volume: 5–50 mL. Magnetic particle concentrators are designed to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids).

Note that this magnet is for microbiology applications only, not for cell separations.

Dynabeads™ Rotary Mixer (Applied Biosystems™)

A rotisserie-style mixer providing continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids). Inter-changeable rod accommodates different tube sizes and sample volumes. Basic rod designed for 14 X 1.5 ml tubes or 12 X 10-15 ml tubes. Additional rod (Cat. No. 947-02) available for 14 X 30-50 ml tubes.

KingFisher™ mL Food Protection Purification System (Thermo Scientific™)

Carry out rapid purification and concentration of selected targets from a broad range of pre-enriched food samples with the Thermo Scientific KingFisher mL Food Protection Purification System. Combined with Dynabeads immuno-magnetic separation (IMS) products, the system provides an optimized solution for the purification of microorganisms prior to detection using methods such as PCR, ELISA, and agar plates.

Features of the KingFisher mL Food Protection Purification System include:
• Protocols included for E. Coli 0157, EPEC/VTEC, listeria, and salmonella
• Automated processing of up to 15 samples in ~40 min
• High reproducibility and maximum performance of capture technology
• Simple, easy operation: complete processing through internal program
• Small footprint: uses only 11.8 in (30 cm) of bench depth

The system-of-choice for processing volumes up to 1 mL, this magnetic particle processor enables all purification steps in a single-strip of five tubes, and supports 15 processed samples per run. With the ability to release target molecules into a volume as small as 50 µL, samples such as DNA or RNA can be isolated and concentrated from larger starting volumes simultaneously.

Dynabeads™ MPC™-S (Magnetic Particle Concentrator) (Applied Biosystems™)

Our Magnetic Particle Concentrators (MPC®) enable optimal binding of Dynabeads® magnetic beads with their targets (e.g., cells, proteins, and nucleic acids) in a variety of starting samples. The MPC®-S was designed specifically for microbiology applications; it has been validated with select Dynabeads® for enrichment of E. coli O157:H7, Salmonella, non-O157 STEC, Cryptosporidium, and Giardia. The MPC®-S can be used with both food enrichment samples types as well as water samples. It holds up to six microcentrifuge tubes and has an optimal working volume range of 20 µl–2 ml.

Note that this magnet is for microbiology applications only, not for eukaryotic cell separations. For use in food and environmental testing only. Not for any animal or human therapeutic or diagnostic use.

Dynabeads™ MX MPC™-S Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold two MPC®-S sample racks (Cat. No. A13346); capacity up to 12 microcentrifuge tubes.

Dynabeads™ EPEC/VTEC O145 (Applied Biosystems™)

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever.

This Product Contains: Dynabeads coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ MX Mixer Base (Applied Biosystems™)

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins and nucleic acids). Variable speed (5-40 rpm). Can be operated in both cold and warm conditions (4-37°C). Inter-changeable heads and a turret unit are designed to attach to the mixer base and enable usage with different tube sizes and sample volumes.

Dynabeads™ MX 12-tube Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold 12 sample tubes ranging from 1.5 ml (microcentrifuge) to 1.7 mm diameter. Requires use of Mixer Turret (Cat. No. 159-06) to affix to Mixer Base.

Dynabeads™ Rotary Mixer Rod (Applied Biosystems™)

A mixing rod designed for use with the Dynabeads® Rotary Mixer (Cat. No. 947-01). Holds 14 X 30-50 ml tubes.

Dynabeads™ L10 Tubes (Applied Biosystems™)

Flat-sided reusable glass tubes necessary for optimal recovery of cysts/oocysts during magnetic separation with the MPC-1 or MPC-6 magnetic particle concentrators when using Dynabeads® anti-Cryptosporidium or Dynabeads® GC-Combo.

Dynabeads™ MAX EPEC/VTEC O121 Kit (Applied Biosystems™)

The Dynabeads MAX EPEC/VTEC O121 Kit allows for the rapid, selective concentration of E. coli O121 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Sensitivity
Following the Dynabeads MAX EPEC/VTEC O121 protocol, the presence or absence of one viable E. coli O121 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

NOTE: Lot 1375851 of this product contains only 1 vial of paramagnetic beads (1 mL each) sufficient for 100 reactions. This batch follows a slightly modified protocol (see use instructions in document part number MAN0007304 Revision 1.0).

Dynabeads™ MX1 Mixer (Applied Biosystems™)

The Dynabeads® MX1 Mixer combines Dynabeads® MX Mixer Base (Cat. No. 159-02), Mixer Turret (Cat. No. 159-06), and 12-tube Mixing Head (Cat. No. 159-03). Designed for sample tubes ranging from 1.5 ml (microcentrifuge) to 1.7 mm diameter.

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, nucleic acids). Inter-changeable heads accommodate different tube sizes and sample volumes.