Dynabeads IMS Reagents & KingFisher mL Food Protection Purification System

Dynabeads™ EPEC/VTEC O145 (Applied Biosystems™)

For the rapid selective concentration of E.coli O145. Method can be performed manually or can be automated by using a BeadRetriever.

This Product Contains: Dynabeads coated with anti-E.coli O145 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ MPC™-1 (Magnetic Particle Concentrator) (Applied Biosystems™)

The MPC®-1 (Magnetic Particle Concentrator) holds one tube of variable diameter (10–30 mm, 5–50 mL tube). Optimal working volume: 5–50 mL. Magnetic particle concentrators are designed to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids).

Note that this magnet is for microbiology applications only, not for cell separations.

Dynabeads™ EPEC/VTEC O111 (Applied Biosystems™)

For the rapid selective concentration of E.coli 0111. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

This Product Contains: Dynabeads coated with anti-E.coli 0111 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ Rotary Mixer Mixing Platform (Applied Biosystems™)

When attached to the Dynabeads® Rotary Mixer (Cat. No. 947-01), the Mixing Platform can hold Dynabeads in 3 MPC®-S racks or one 96-well microcentrifuge tube holder provided with the Mixing Platform. Variable speed (15-80 rpm). Can be operated in both cold and warm conditions (4-37°C).

Dynabeads™ EPEC/VTEC O26 (Applied Biosystems™)

For the rapid selective concentration of E.coli 026. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

This Product Contains: Dynabeads coated with anti-E.coli O26 antibodies

Sensitivity: Sensitivity is very high and enables detection of 1 viable organism in 25g of sample or as few as 100 organisms / ml after pre enrichment

Dynabeads™ MAX E. coli O157 Kit (Applied Biosystems™)

Dynabeads MAX E.Coli O157 provides a powerful sample preparation method to improve PCR detection and quantification of E.Coli O157:H7 in food, feces, water and other environmental samples.
Based on well known and widely used immunomagnetic separation (IMS) technology, Dynabeads MAX E.Coli O157 provides a selective E.Coli O157:H7 capture step that delivers significant improvements in PCR analytical process time, as well as the sensitivity and consistency of results. With Dynabeads MAX E.Coli O157, you'll:

• Perform high throughput, rapid, and effective E.Coli O157:H7 sample preparation
• Significantly shorten sample pre-enrichment time
• Improve detection sensitivity in PCR sample analysis
• Significantly reduce overall analysis time
• Can be carried out manually or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Dynabeads™ anti-Listeria (Applied Biosystems™)

For the selective enrichment of Listeria directly from pre-enrichment samples using ImmunoMagnetic Separation. Method can be performed manually or can be automated by using a Thermo Scientific KingFisher mL Food Protection Purification System.

Benefits and features

Saves processing and confirmation time

• Increases sensitivity and positive isolation rate
• Easy IMS protocol - 30 minutes from start to finish
• Shelf stable reagent and low start up costs

This Product Contains: Dynabeads coated with anti-Listeria antibodies

Dynabeads™ Rotary Mixer (Applied Biosystems™)

A rotisserie-style mixer providing continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids). Inter-changeable rod accommodates different tube sizes and sample volumes. Basic rod designed for 14 X 1.5 ml tubes or 12 X 10-15 ml tubes. Additional rod (Cat. No. 947-02) available for 14 X 30-50 ml tubes.

KingFisher™ mL Food Protection Purification System Tubes & Tips (Applied Biosystems™)

The tube strips are designed for processing and isolation of target molecules; one sample uses one tube strip which is made up of five tubes. The disposable plastic tips are designed for shielding the magnetic surface from contact with the sample. The KingFisher mL Food Protection Purification System utilizes low binding, disposable plastics materials for optimal magnetic particle processing.

Dynabeads™ MX MPC™-S Mixing Wheel (Applied Biosystems™)

Attachment for Dynabeads® MX Mixer Base (Cat. No. 159-02). Designed to hold two MPC®-S sample racks (Cat. No. A13346); capacity up to 12 microcentrifuge tubes.

Dynabeads™ MX1 Mixer (Applied Biosystems™)

The Dynabeads® MX1 Mixer combines Dynabeads® MX Mixer Base (Cat. No. 159-02), Mixer Turret (Cat. No. 159-06), and 12-tube Mixing Head (Cat. No. 159-03). Designed for sample tubes ranging from 1.5 ml (microcentrifuge) to 1.7 mm diameter.

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, nucleic acids). Inter-changeable heads accommodate different tube sizes and sample volumes.

MPC™-6 (Magnetic Particle Concentrator)

The MPC®-6 (Magnetic Particle Concentrator) holds six tubes with variable diameters (10–30 mm). Optimal working volume: 5–15 mL. Magnetic particle concentrators are designed to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, and nucleic acids).

Dynabeads™ MAX EPEC/VTEC O45 Kit (Applied Biosystems™)

The Dynabeads MAX EPEC/VTEC O45 Kit allows for the rapid, selective concentration of E. coli O45 serogroup bacteria in food and environmental samples to improve downstream detection via PCR or culture methods.

• Improve detection sensitivity in PCR sample analysis
• Reduce background in selective agar detection
• Perform the method manually or automate using the Thermo Scientific KingFisher mL Food Protection Purification System System

Improve Sensitivity and Reduce Background
Highly specific antibodies attached to paramagnetic beads are incubated with an aliquot of pre-enriched sample. The beads bind and concentrate the target bacteria from non-target organisms. Washing steps help limit the carryover of inhibitors and matrix-associated particulates. These steps combine to improve the sensitivity of PCR-based detection while reducing background for plating methods.

Manual or Automated Processing
The immunomagnetic separation (IMS) steps can be performed manually with magnets and mixers or automated using the Thermo Scientific KingFisher mL Food Protection Purification System.

Sensitivity
Following the Dynabeads MAX EPEC/VTEC O45 protocol, the presence or absence of one viable E. coli O45 can be determined in the sample sizes described if this one E. coli cell is able to replicate and is not masked by resident background flora.

Dynabeads™ MX Mixer Base (Applied Biosystems™)

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins and nucleic acids). Variable speed (5-40 rpm). Can be operated in both cold and warm conditions (4-37°C). Inter-changeable heads and a turret unit are designed to attach to the mixer base and enable usage with different tube sizes and sample volumes.

Dynabeads™ MX2 Mixer (Applied Biosystems™)

The Dynabeads® MX2 Mixer combines Dynabeads® MX Mixer Base (Cat. No. 159-02), Mixer Turret (Cat. No. 159-06), and 8-tube Mixing Wheel (Cat. No. 159-04). Designed for sample tubes ranging from 15 to 27 mm in diameter.

Dynabeads® MX mixers provide continuous gentle motion to obtain optimal binding of Dynabeads® with targets (e.g., cells, proteins, nucleic acids). Variable speed (5-40 rpm). Can be operated in both cold and warm conditions (4-37°C). Inter-changeable heads accommodate different tube sizes and sample volumes.