February 2020 New Products

GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells (Invitrogen™)

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features of the GeneArt Gibson Assembly HiFi Cloning Kit include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
• GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells) (this page)
GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells)

PowerTrack™ SYBR Green Master Mix (Applied Biosystems™)

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up. Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

Features of PowerTrack SYBR Green Master Mix include:
• Built-in visual indicators aid in reaction setup
• Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization
• Specific and tight reproducibility in Ct’s over a broad dynamic range
• Formulated with UNG/dUTP to prevent contamination of carryover PCR products

Built-in visual indicators
PowerTrack SYBR Green Master Mix contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well. A separate sample buffer is provided that contains an inert yellow dye. The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes. The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with PowerTrack SYBR Green Master Mix.

Formulated for maximum specificity and reproducibility
PowerTrack SYBR Green Master Mix contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification. PowerTrack SYBR Green Master Mix is designed for exceptional performance in your most challenging real-time PCR applications.

Broad instrument compatibility
PowerTrack SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments. It is also compatible with the Bio-Rad CFX96™, CFX384™, and iQ™5; Roche LightCycler™ 480; and Agilent Mx3005P™ systems. For optimal results, primer concentrations should be in the range of 300–800 nM.

UNG for carryover contamination control
Contamination in labs that routinely run PCR is a major concern for many researchers and is the source of most false positives. The inclusion of UNG and dUTP in PowerTrack SYBR Green Master Mix results in the degradation of any previously amplified PCR products and helps prevent contamination of subsequent qPCR reactions.

4N6FLOQSwabs®, regular tip, tube, active drying system (Applied Biosystems™)

4N6FLOQSwabs, regular tip, are comprised of a solid, molded plastic applicator shaft with a regular-sized tip. The tip of the applicator is coated with short Nylon fibers that are arranged in a perpendicular fashion. This perpendicular arrangement results from a process called flocking, where the fibers are sprayed onto the tip of the swab while it is held in an electrostatic field. This process creates a highly absorbent, thin layer with an open structure. The swab is supplied in a tube with an active drying system for sample drying and is suitable for reference and evidence collection applications.

Key features:
• Certified free of DNase, RNase, and amplifiable human DNA
• Rapid absorption capability and superior sample release
• Regular-size tip for buccal cell collection
• Break point (from tip end): 20 mm

Unlike traditional fiber wound swabs, 4N6FLOQSwabs have no internal absorbent core to disperse and entrap the specimen—the sample stays close to the surface for faster and more efficient elution. The perpendicular Nylon fibers act like a soft brush that facilitates improved collection of cellular material. Capillary action between the fiber strands facilitates strong hydraulic uptake of liquid sample, and the sample stays close to the surface allowing easy elution.

4N6FLOQSwabs are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

CytKick™ Max Autosampler (Invitrogen™)

The CytKick Max Autosampler is for use with the Attune NxT Acoustic Focusing Cytometer. It enables rapid processing of multiple samples by automatically analyzing samples from 96- and 384-well plates (standard and deep), as well as some custom plate types and microfuge tube racks. The CytKick Max Autosample features a boost mode that enables faster sample processing, as well as passive cooling for some plates/racks. A CytKick Max Accessory Kit is included that contains four accessory plates: two cooling plates and two tube adaptors, as well as a Max dongle key. A computer is not included with the autosampler since one is provided with the cytometer.

Features of the CytKick Max Autosampler include:
Reliable—intelligent probe design minimizes clogging and carryover (0.5%) and prevents damage to the instrument
Mixing by aspiration—mixing of sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability
Easy transition—no disassembly, no additional QC, no reboot required for conversion between plates and tubes
Boost mode—reduces processing time from 42 min to 22 min for 96-well plate (using one rinse and one mix and full analysis of a 20-µL sample)
Sample cooling—passive cooling available for 96-well U-bottom plates and microfuge tube racks
Maximum compatibility—96- and 384-well plates (standard and deep), 96- and 384-well standard-depth foil-covered plates, some custom plate types, and 1.5-mL and 2-mL microfuge tube racks (up to 24 per rack)
Auto-cleaning—performs automated cleaning when the instrument is shutting down

High-throughput mode
The CytKick Max Autosampler has two options for processing samples in a high-throughput environment: high-throughput mode and boost mode. High-throughput mode enables plate screening by collecting 20 µL of sample at a rate of 1000 µL/min, while Boost mode shortens the time between samples and allows the user to select the sample volume and sample input rate.

Reliable
The CytKick Max Autosampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelligent Probe, with a 200-micron diameter that is larger than most probes available in the industry, minimizes instances of clogging.

To prevent probe crashes, which may require expensive probe replacement, the CytKick Max Autosampler automatically performs a probe self-calibration to help ensure consistent positioning of the probe within every well. The self-calibration verifies proper function of the probe, reduces variability in probe positioning over time to prevent failures during plate runs, and helps improve reliability over the life of the system. Self-calibration of the probe also allows the autosampler to be easily moved, so it can be shared between multiple instruments.

Sample cooling
The CytKick Max Autosampler is compatible with a 96-well standard-plate universal cooling block that will keep samples at 2-8°C for ~60 minutes, a 96-well standard-plate extended cooling block that will keep samples at 2-8°C for ~100 minutes, and a microfuge-tube cooling rack that will keep samples at 2-8°C for ~100 minutes. The user will need to chill the cooling block/rack in the refrigerator overnight prior to use.

Maximum compatibility
The CytKick Max Autosampler allows use of many different plate formats, including 96-, 384-, and deep-well plates, without being restricted to a single manufacturer. The CytKick Max Autosampler also enables 1.5- and 2.0-mL microfuge tubes to be run in an automated format. In addition, the autosampler initiates automated cleaning procedures when the instrument is shutting down to help ensure proper maintenance with little user interaction.

Mixing by aspiration
Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the CytKick Max Autosampler mixes samples by aspiration and stirring, enabling uniform mixing while preserving sample viability and resulting in consistent data with CVs less than 10% across the plate. The CytKick Max Autosampler also has a gentle mix mode for fragile cell lines.

Easy to transition
To maintain system flexibility, the manual sample probe is always accessible even when the loader is present, and switching between tubes and plates is detected via software and does not require additional user intervention.

Simple to operate
The instrument design and software were developed with customer ease-of-use in mind. The CytKick Max Autosampler has fluidic bottles with sensors that inform the user when the fluids need to be replaced. The autosampler’s indicator lights and warning systems have been designed to ensure that a user who has walked away from the system can easily see the system status at all times.

Setting up an experiment with the Attune NxT cytometric software is simple. Using a plate layout view, users can create compensation wells, define instrument settings, and identify samples. The intuitive software allows quick learning and data analysis. For example, a heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data.

Autocleaning of autosampler is done during the instrument shutdown process therefore no additional user steps are required to maintain system.

GeneArt™ Gibson Assembly HiFi Master Mix (Invitrogen™)

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Master Mix kit includes master mix, positive control, and water and accommodates the use of your own competent cells.

Features of the GeneArt Gibson Assembly HiFi Master Mix include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
• GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells) (this page)

CytoScan™ HT-CMA 96F Assay Training Kit (Applied Biosystems™)

The Applied Biosystems CytoScan HT-CMA 96F Assay Kit enables the detection of chromosomal abnormalities such as deletions, duplications, and uniparental disomy (UPD), and does so in a high-throughput, automated format. The assay is designed for use with genomic DNA from blood or amniotic fluid samples. This CytoScan HT-CMA Assay Training Kit contains arrays, reagents, and sample plates sufficient for the FAS led training week as well as the following proficiency run.

Features of the CytoScan HT-CMA 96F Assay Kit include:
• Consolidated testing with a dual CNV and SNV design
• Increased sample throughput with a fully automated assay protocol
• Fast and easy high-resolution genome-wide copy number analysis with the free Chromosome Analysis Suite software

High-resolution genome-wide copy number and SNV analysis in one
The CytoScan HT-CMA assay enables cytogenetic researchers to detect and analyze relevant chromosomal aberrations with confidence. The assay provides high-resolution coverage of OMIM and DECIPHER genes, along with high-density SNP coverage for loss of heterozygosity (LOH) and UPD detection. The CytoScan HT-CMA assay also includes up to 178 relevant single nucleotide variants (SNV) and content for analysis of SMN1, which allows for consolidation of testing.

High-throughput array format and full automation
Bring consistency to your cytogenetic analysis and free up technician time with the fully automated CytoScan HT-CMA assay. Preparation of gDNA for hybridization onto the arrays is automated on the NIMBUS Target Preparation Instrument, with automated array processing occurring on the GeneTitan MC Instrument.

Chromosome Analysis Suite-designed for cytogenetic applications
The popular Chromosome Analysis Suite (ChAS) software is available free of charge as part of the CytoScan HT-CMA assay. ChAS software is tailored to cytogenetic research analysis and reporting with:
• Streamlined analysis and reporting workflow
• Ability to apply customized filters to analyze the genome at different levels of resolution
• Options to create, modify, and upload annotation files and flag regions for focused analysis
• A database for storage, query, and visualization of samples
• Direct access to external databases such as NCBI, UCSC Genome Browser, Ensembl, and OMIM
• Trio consistency checking for parental studies

The new standard in high-throughput cytogenetic analysis
The combined high resolution DNA copy number data and the ability to screen a panel of SNVs on a single array makes the CytoScan HT-CMA assay the new standard for high throughput cytogenetics analysis.

GeneArt™ Gibson Assembly EX Master Mix (Invitrogen™)

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Master Mix kit includes master mix, positive control, and water, and accommodates the use of your own competent cells.

Features of the GeneArt Gibson Assembly EX Master Mix include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
GeneArt Gibson Assembly EX Cloning Kit, EC (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly EX Cloning Kit, CC (with One Shot TOP10 chemically competent cells)
• GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells) (this page)

TaqMan™ Fast Advanced Master Mix, no UNG (Applied Biosystems™)

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in a short time. It provides best-in-class performance, including the widest quantifiable range, in both single and duplex reactions, even with challenging targets. TaqMan Fast Advanced Master Mix contains AmpliTaq Fast DNA Polymerase, dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. It is supplied at a 2X concentration. Note: This TaqMan Fast Advanced Master Mix does not contain uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Fast Advanced Master Mix products, with and without UNG.

TaqMan Fast Advanced Master Mix features include:
Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
Reduced run times (<40 minutes) on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
Seamlessly transitions into your experiments—validated with TaqMan Assays for gene expression and microRNAs, and TaqMan array micro-fluidic cards

Best-in-class performance
TaqMan Fast Advanced Master Mix has been designed to provide performance equal to or better than standard master mixes. It has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. TaqMan Fast Advanced Master Mix’s dynamic range (up to 7 logs) is the widest in the industry. The impressive sensitivity of the mix is showcased in the figure below which compares the Ct values of TaqMan Fast Advanced Master Mix and TaqMan Universal PCR Master Mix across a panel of gene expression assays.

Benchtop stability for high-throughput handling and convenience
TaqMan Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. The figure below shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.

For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running plates upon assembly.

Optimized for multiplexing
We realize that confidence is paramount when it comes to your results. TaqMan Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). The figure below shows results for the experimental target β-actin (gene name: ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.

Validated for microRNA assays
TaqMan Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in the figure below demonstrate excellent PCR linearity over a 6-log range of input template.

Reduced run times on standard instrumentation
TaqMan Fast Advanced Master Mix has been optimized for use with both fast and standard instrumentation, enabling researchers who currently own standard instruments to also reap the performance benefits and time savings this mix provides. The figure below showcases the impressive results achieved when using TaqMan Fast Advanced Master Mix under fast thermal cycling conditions on the Applied Biosystems 7300 Real-Time PCR System. The mix has been tested with all Applied Biosystems standard real-time PCR instrumentation to enable success independent of whether or not you own a fast-enabled instrument.

SuperScript™ IV CellsDirect™ Lysis Reagents (Invitrogen™)

The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples. See the SuperScript IV CellsDirect cDNA Synthesis Kit manual for protocol recommendations.

SuperScript IV CellsDirect Lysis Reagents are optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating RNA.

• Ready-to-use pre-mixed cell lysis solution compatible with a variety of cell-types
• Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA

CaptureSelect™ FcXP Affinity Matrix (Thermo Scientific™)

CaptureSelect FcXP Affinity Matrix is the next-generation CaptureSelect FcXL matrix, specifically designed for higher binding capacity. The selectivity remains identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix, but the binding capacity is increased (>40 g IgG/L).

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human-specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived

CaptureSelect FcXP Affinity Matrix can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enables a robust and efficient purification process with excellent purity obtained in one step.

View other research scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13-kDa single-domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen-binding domain, efficiently produced in Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXP affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl2, 40% (v/v) propylene glycol, pH 7
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells (Invitrogen™)

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells, is a complete kit that includes master mix, positive control, water, and One Shot TOP10 chemically competent E. coli.

Features of the GeneArt Gibson Assembly HiFi Cloning Kit include:
Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
Efficient—high fidelity provides more correct clones than other methods
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
• GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells) (this page)
GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells)

Olympus™ 20X Objective, Fluorite, NA 0.70, WD 0.8–1.8 mm, correction collar (vessel thickness 0–1.6 mm) (Invitrogen™)

This fluorite (semi-apochromat) objective is equipped with an adjustable collar that affords flexibility in imaging through vessels with a thickness from 0 (no coverslip) to 2.0 mm. With four-color correction for spherical and chromatic aberrations, it delivers exceptional image quality. For the sharpest image simply set the collar to the thickness of the vessel/coverslip you are using. If the thickness is unknown, turn the collar in either direction while monitoring the sample focus. All Olympus objectives are compatible with EVOS cell imaging systems and offer outstanding optical performance from visible to near-infrared light. The extensive selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this Olympus objective:
• Magnification: 20X
• Numerical Aperture: 0.70
• Working Distance: 0.8–1.8 mm
• Superb image quality

Image quality
Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation. High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide.
Explore the entire EVOS line of imaging systems and accessories ›

CytoScan™ HT-CMA 96F Assay Kit (Applied Biosystems™)

The Applied Biosystems CytoScan HT-CMA 96F Assay Kit enables the detection of chromosomal abnormalities such as deletions, duplications, and uniparental disomy (UPD), and does so in a high-throughput, automated format. The assay is designed for use with genomic DNA from blood or amniotic fluid samples. Please see the CytoScan HT-CMA Assay Training Kit for arrays, reagents, and sample plates sufficient for the FAS led training week as well as the following proficiency run.

Features of the CytoScan HT-CMA 96F Assay Kit include:
• Consolidated testing with a dual CNV and SNV design
• Increased sample throughput with a fully automated assay protocol
• Fast and easy high-resolution genome-wide copy number analysis with the free Chromosome Analysis Suite software

High-resolution genome-wide copy number and SNV analysis in one
The CytoScan HT-CMA assay enables cytogenetic researchers to detect and analyze relevant chromosomal aberrations with confidence. The assay provides high-resolution coverage of OMIM and DECIPHER genes, along with high-density SNP coverage for loss of heterozygosity (LOH) and UPD detection. The CytoScan HT-CMA assay also includes up to 178 relevant single nucleotide variants (SNV) and content for analysis of SMN1, which allows for consolidation of testing.

High-throughput array format and full automation
Bring consistency to your cytogenetic analysis and free up technician time with the fully automated CytoScan HT-CMA assay. Preparation of gDNA for hybridization onto the arrays is automated on the NIMBUS Target Preparation Instrument, with automated array processing occurring on the GeneTitan MC Instrument.

Chromosome Analysis Suite-designed for cytogenetic applications
The popular Chromosome Analysis Suite (ChAS) software is available free of charge as part of the CytoScan HT-CMA assay. ChAS software is tailored to cytogenetic research analysis and reporting with:
• Streamlined analysis and reporting workflow
• Ability to apply customized filters to analyze the genome at different levels of resolution
• Options to create, modify, and upload annotation files and flag regions for focused analysis
• A database for storage, query, and visualization of samples
• Direct access to external databases such as NCBI, UCSC Genome Browser, Ensembl, and OMIM
• Trio consistency checking for parental studies

The new standard in high-throughput cytogenetic analysis
The combined high resolution DNA copy number data and the ability to screen a panel of SNVs on a single array makes the CytoScan HT-CMA assay the new standard for high throughput cytogenetics analysis.

4N6FLOQSwabs® Buccal Lollipop, single-sided, peelpouch (Applied Biosystems™)

4N6FLOQSwabs Buccal Lollipops, single-sided, have a solid molded plastic applicator shaft with a round-shaped tip to improve collection of buccal cells from the inside of a subject’s check. One side of the tip of the 4N6FLOQSwabs Buccal Lollipop are coated with short Nylon fibers arranged in a perpendicular fashion to create a highly absorbent thin layer with an open structure. Collection of cellular material on one side of the lollipop allows for increased transfer efficiency of the sample. The perpendicular Nylon fibers act like a soft brush that facilitates improved collection of cellular material. Capillary action between the fiber strands facilitates strong hydraulic uptake of liquid sample, and the sample stays close to the surface for easy elution.

Key features:
• Certified free of DNase, RNase, and amplifiable human DNA
• Rapid absorption capability and superior sample release
• Single sided lollipop for buccal cell collection
• Individually packaged in a convenient peel pouch format

4N6FLOQSwabs Buccal Lollipops are manufactured by Copan, a market leader in the area of sample collection tools, and distributed globally by Thermo Fisher Scientific.

GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells (Invitrogen™)

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features of the GeneArt Gibson Assembly EX Cloning Kit include:
Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
Efficient—robust efficiency provides successful clones for both simple and challenging constructs
Flexible—design guidelines allow assembly into any vector of your choice
Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments
GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility
The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

Convenient formats
GeneArt Gibson Assembly EX kits are available in multiple formats, including:
• GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells) (this page)
GeneArt Gibson Assembly EX Cloning Kit, chemically competent cells (with One Shot TOP10 chemically competent cells)
GeneArt Gibson Assembly EX Master Mix kits (cloning kit without the cells)