GMO PCR Testing Products

TaqMan™ Roundup Ready Soya Quantification Kit

The TaqMan® Roundup Ready Soya Quantification Kit is a food safety testing kit used to determine the percentage of Roundup Ready soya against the total amount of soya present in a DNA sample. This real-time PCR kit contains all reagents (except for the template DNA) required for the quantification of Roundup Ready soya. The benefits of the TaqMan® Roundup Ready Soya Quantification Kit over other commercially available products include:

• Efficiency—detection of small DNA fragments through use of Taqman® MGB probes
• Sensitivity—quantification limit of 20 DNA copies and detection limit of 3 DNA copies
• Flexibility—adapted to processed DNAs

The TaqMan® Roundup Ready Soya Quantification Kit consists of plasmid-containing targets for the endogenous soy gene, known as lectin, and for the transgenic event (GTS-40-3-2) present in Roundup Ready soy. This soy accounts for approximately 80% of all soy cultivated worldwide and is considered to be the most widespread transgenic event.

The TaqMan® Roundup Ready Soya Quantification Kit is optimized for use in all Applied Biosystems® real-time PCR instruments.

The development and manufacture of this kit occurs through a partnership with IMEGEN (Instituto de Medicina Genomica), which has a long history of GMO research and development.

Other related products include:
GMO Extraction Kit
TaqMan® GMO Screening Kit
TaqMan® GMO Maize Quantification Kit

TaqMan™ GMO Maize Quantification Kit

The Taqman® GMO Maize Quantification Kit is a food safety testing kit used to determine the number of copies of P35S promoter present in genetically modified organisms compared to the number of copies of maize present in any food or feed sample. The benefits of the Taqman® GMO Maize Quantification Kit over other commercially available products include:

• Efficiency—detection of small DNA fragments through use of Taqman® MGB probes
• Sensitivity—quantification limit of 20 DNA copies; detection limit of 3 DNA copies (maize and P355 systems)
• Flexibility—adapted to processed DNAs

The Taqman® GMO Maize Quantification Kit is effective in detecting the presence of genetically modified organisms through transgenic maize quantification, based on the detection of P35S promoter. The detection success of this promoter is due to this regulatory region being present in most of the transgenic events. In addition, the Taqman® GMO Maize Quantification Kit contains all necessary reagents for quantification and is optimized for use in all (Applied Biosystems®) real-time PCR instruments.

The development and manufacture of this kit occurs through a partnership with IMEGEN (Instituto de Medicina Genomica), which has a long history of GMO research and development.

Other related products include:
GMO Extraction Kit
TaqMan® GMO Screening Kit
Taqman® Roundup Ready Soya Quantification Kit

TaqMan™ GMO Screening Kit

The TaqMan® GMO Screening Kit analyzes DNA extracted from food and feed samples for the presence of the main transgenic regulatory regions. This real-time PCR kit contains all reagents (except for the template DNA) required for the detection of genetically modified organisms (GMOs ). In addition, the kit contains an Internal Positive Control (IPC) to identify possible PCR inhibition. The benefits of the Taqman® GMO Screening Kit over other commercially available products include:

• Flexibility—GMOs detected in DNA obtained from ANY food or feed source
• Accuracy—an internal positive control (IPC) rules out inhibition during qPCR process
• Speed—results provided in less than 3 hours

The TaqMan® GMO Screening Kit is capable of multiplex amplification of Endogenous Vegetal Control, Internal Positive Control, and these regulatory regions:

• P35S/CaMV
• TNOS/A. tumefaciens
• P34S/FMV

All of which make possible the detection of significant events such as soya MON89788.

The TaqMan® GMO Screening Kit provides all of the necessary reagents for testing 48 samples and is optimized for use in all Applied Biosystems® real-time PCR instruments. And because it is a real-time PCR kit, no electrophoresis is required. In addition, the kit offers extremely high sensitivity, detecting targets with only 3 DNA copies.

The development and manufacture of this kit occurs through a partnership with IMEGEN (Instituto de Medicina Genomica), which has a long history of GMO research and development.

Other related products include:
Taqman® Roundup Ready Soya Quantification Kit
GMO Extraction Kit
TaqMan® GMO Maize Quantification Kit

GMO Extraction Kit

The GMO Extraction Kit enables fast and easy purification of DNA for use in food safety testing for genetically modified organisms. The kit is capable of isolating highly purified DNA from a wide variety of different sample types without the use of toxic reagents. The benefits of the GMO Extraction Kit over other commercially-available products include:

• Performance—isolation of extremely pure DNA (A260/A280 >1.8)
• Specificity—adapted to food samples for transgenic material analysis
• Efficiency—total assay time is less than 2 hours, requiring minimal handling steps

The GMO Extraction Kit employs silica-based DNA purification using spin column technology to provide samples for GMO testing. The kit has been specifically adapted for transgenic material analysis of a wide array of sample matrices, including raw materials, processed food, and feed. Effectively removing inhibitors, the GMO Extraction Kit provides isolated, highly-purified DNA, quickly, reliably, and safely.

The development and manufacture of this kit occurs through a partnership with IMEGEN (Instituto de Medicina Genomica), which has a long history of GMO research and development.

Other related products include:
Taqman® Roundup Ready Soya Quantification Kit
TaqMan® GMO Screening Kit
TaqMan® GMO Maize Quantification Kit

PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing (Applied Biosystems™)

The PrepSEQ® Nucleic Acid Extraction Kit produces high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples. The quantity and quality of the DNA can greatly impact the success of the sample analysis process and the overall quality of the final result. Food and environmental samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ™ Nucleic Acid Extraction Kit meets these needs.

Automated Sample Preparation for PCR-Based Assays
• Extract high-quality bacterial DNA
• Process a wide range of food and environmental samples
• Fast time-to-results
• Prepare hundreds of samples per shift per instrument

High-Throughput Food and Environmental Sample Preparation
PCR-based bacterial assays have become an important tool in food safety and environmental testing. The Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit prepares high-quality bacterial DNA from food and environmental samples for PCR-based testing using magnetic bead-based extraction. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Streamlined Workflow
An easy-to-use, easy-to-learn protocol simplifies integration of the PrepSEQ™ Nucleic Acid Extraction Kit into the laboratory workflow. The same protocol can be used on a wide range of sample types, and the MagMAX™ Express-96 instrument automates many of the workflow steps to reduce hands-on time.

A Complete Solution
The PrepSEQ™ Nucleic Acid Extraction Kit is part of a fully integrated, start-to-finish solution for food and environmental testing. The kit has been performance-verified for food and environmental testing with MicroSEQ® Microbial Detection Kits, the Applied Biosystems® 7500 Fast Real-Time PCR System, and RapidFinder™ Express Software.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.