July 2019 New Products

E-Gel™ Agarose Gels with SYBR™ Safe DNA Gel Stain, 1% (Invitrogen™)

Invitrogen E-Gel precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis.Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or DNA fragment libraries.

Features of E-Gel agarose gels include:
Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
In-process control—view DNA sample migration in real time
Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.

Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.

Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.

Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.

Learn more about the E-Gel Power Snap Electrophoresis System ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide

E-Gel™ Agarose Gels with SYBR™ Safe DNA Gel Stain, 2% (Invitrogen™)

Invitrogen E-Gel precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis.Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or DNA fragment libraries.

Features of E-Gel agarose gels include:
Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
In-process control—view DNA sample migration in real time
Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.

Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.

Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.

Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.

Learn more about the E-Gel Power Snap Electrophoresis System ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide

GFAP Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human GFAP Simplex ProcartaPlex kit measures GFAP protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Reported application: multiplex immunoassay

E-Gel™ Agarose Gels with SYBR™ Safe DNA Gel Stain, 4% (Invitrogen™)

Invitrogen E-Gel precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis.Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or DNA fragment libraries.

Features of E-Gel agarose gels include:
Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
In-process control—view DNA sample migration in real time
Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.

Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.

Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.

Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.

Learn more about the E-Gel Power Snap Electrophoresis System ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide

Neurofilament heavy (NF-H) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Neurofilament heavy (NF-H) Simplex ProcartaPlex kit measures NF-H protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Reported application: multiplex immunoassay

E-Gel™ 48 Agarose Gels with SYBR™ Safe DNA Gel Stain, 1% (Invitrogen™)

Invitrogen E-Gel 48 high-throughput precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis. Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run.

Each gel contains 48 sample wells and 4 marker wells in 1% or 2% high-resolution agarose with a 3.2-cm run length. The various agarose percentages are used to separate DNA fragments of different sizes:
• 1% agarose: DNA fragments between 400 bp and 10 kb
• 2% agarose: DNA fragments between 50 bp and 3 kb

Each cassette is individually labeled with an EAN39 bar code that is recognized by most commercially available robotic bar code readers.

Features of E-Gel 48 gels include:
High throughput analysis—contains 48 sample lanes and 4 marker lanes per gel
Automation compatible—suitable for use with multi-channel pipettors and commonly used 8- and 12-pin liquid handling robots
Scalable—resolve up to 192 sample in one run by combining up to four E-Base Mother and Daughter devices
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~20 minutes

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Unlike ethidium bromide, the DNA stain most commonly incorporated into agarose gels, SYBR Safe DNA Gel Stain is a non-toxic, non-mutagenic DNA stain that is safer for the user, safer for the environment, and can save institutional overhead costs for disposal.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels.

Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel straining steps. Just load your samples and run.

Nucleic acid markers
For optimal DNA analysis on E-Gel 48 agarose gels we recommend the specially formulated E-Gel 96 High Range DNA Ladder (Cat. No. 12352019) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
High-throughput E-Gel agarose gels require the E-Base Electrophoresis Device for gel running.

Learn more about the E-Base Device ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The high-throughput E-Gel 96 and 48 agarose gels with SYBR Safe are available in 1% and 2% gel percentages. E-Gel agarose gel selection guide

Ion 530™ Chip Kit for Agrigenomics (Ion Torrent™)

The Ion 530 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 530 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of less than 2.5 hours for 200 bp sequencing.

The Ion 530 Chip offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 15–20 million reads
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.

Apolipoprotein E4 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Apolipoprotein E4 Simplex ProcartaPlex kit measures Apolipoprotein E4 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Recommended sample dilution factor: 100 (CSF); 10,000 (serum, plasma)
Reported application: multiplex immunoassay

Complement Factor H Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human Complement Factor H Simplex ProcartaPlex kit measures Complement Factor H protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Recommended sample dilution factor: 100 (CSF); 10,000 (serum, plasma)
Reported application: multiplex immunoassay

UCHL1 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human UCHL1 Simplex ProcartaPlex kit measures UCHL1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Reported application: multiplex immunoassay

E-Gel™ 48 Agarose Gels with SYBR™ Safe DNA Gel Stain, 2% (Invitrogen™)

Invitrogen E-Gel 48 high-throughput precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis. Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run.

Each gel contains 48 sample wells and 4 marker wells in 1% or 2% high-resolution agarose with a 3.2-cm run length. The various agarose percentages are used to separate DNA fragments of different sizes:
• 1% agarose: DNA fragments between 400 bp and 10 kb
• 2% agarose: DNA fragments between 50 bp and 3 kb

Each cassette is individually labeled with an EAN39 bar code that is recognized by most commercially available robotic bar code readers.

Features of E-Gel 48 gels include:
High throughput analysis—contains 48 sample lanes and 4 marker lanes per gel
Automation compatible—suitable for use with multi-channel pipettors and commonly used 8- and 12-pin liquid handling robots
Scalable—resolve up to 192 sample in one run by combining up to four E-Base Mother and Daughter devices
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~20 minutes

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Unlike ethidium bromide, the DNA stain most commonly incorporated into agarose gels, SYBR Safe DNA Gel Stain is a non-toxic, non-mutagenic DNA stain that is safer for the user, safer for the environment, and can save institutional overhead costs for disposal.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels.

Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel straining steps. Just load your samples and run.

Nucleic acid markers
For optimal DNA analysis on E-Gel 48 agarose gels we recommend the specially formulated E-Gel 96 High Range DNA Ladder (Cat. No. 12352019) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
High-throughput E-Gel agarose gels require the E-Base Electrophoresis Device for gel running.

Learn more about the E-Base Device ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The high-throughput E-Gel 96 and 48 agarose gels with SYBR Safe are available in 1% and 2% gel percentages. E-Gel agarose gel selection guide

DH5α Competent Cells (Thermo Scientific™)

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.

• High transformation efficiency: >1x109 cfu/µg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis

Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

Ion 520™ Chip Kit for Agrigenomics (Ion Torrent™)

The Ion 520 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 520 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times in as little as 2.5 hours for 200 bp sequencing.

The Ion 520 Chip Kit offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 3–6 million reads, depending on application
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.

GDNF Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human GDNF Simplex ProcartaPlex kit measures GDNF protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: serum, plasma, CSF
Sample volume: 25 µL (serum, plasma, CSF)
Reported application: multiplex immunoassay

E-Gel™ 96 Agarose Gels with SYBR™ Safe DNA Gel Stain, 1% (Invitrogen™)

Invitrogen E-Gel 96 high-throughput precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis. Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run.

The unique 96-well staggered-well format provides a 1.6-cm run length that resolves DNA fragments between 100 bp and 10 kb. Each gel contains 96 sample lanes and 8 marker lanes, ideal for screening multiple PCR products, plasmid preparations, and restriction digests. The E-Gel 96 loading format is compatible with multi-channel pipettors and the most commonly used 8-, 12-, and 96-pin liquid handling robots. With a 12-minute run time, up to 20,000 samples can be resolved in a single day.

Each cassette is individually labeled with an EAN39 bar code that is recognized by most commercially available robotic bar code readers.

Features of E-Gel 96 gels include:
High throughput analysis—contain 96 sample lanes and 8 marker lanes
Automation compatible—suitable for use with multi-channel pipettors and commonly used 8-, 12-, and 96-pin liquid handling robots
Scalable—resolve up to 384 samples in one run by combining up to four E-Base Mother and Daughter devices
Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
High performance—detect 2–3 ng of sample DNA with a separation time as short as ~12 minutes

Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.

Unlike ethidium bromide, the DNA stain most commonly incorporated into agarose gels, SYBR Safe DNA Gel Stain is a non-toxic, non-mutagenic DNA stain that is safer for the user, safer for the environment, and can save institutional overhead costs for disposal.

Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not require any gel or buffer preparation or gel straining steps. Just load your samples and run.

Nucleic acid markers
For optimal DNA analysis on E-Gel 96 agarose gels we recommend the specially formulated E-Gel 96 High Range DNA Ladder (Cat. No. 12352019) or E-Gel Low Range Quantitative DNA Ladder (Cat. No. 12373031). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Learn more about our E-Gel DNA ladders ›

Running and imaging devices
High-throughput E-Gel agarose gels require the E-Base Electrophoresis Device for gel running.

Learn more about the E-Base Device ›

Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The high-throughput E-Gel 96 and 48 agarose gels with SYBR Safe are available in 1% and 2% gel percentages. E-Gel agarose gel selection guide