March 2019 New Products

ViewRNA™ Tissue Assay Core Kit (Invitrogen™)

The ViewRNA Tissue Assay Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

Learn more about and order ViewRNA Tissue probe sets ›

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

PD-L2 Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse PD-L2 Simplex ProcartaPlex kit measures PD-L2 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: mouse
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: Serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

TrueTag™ Donor DNA Kit, GFP (Invitrogen™)

The Invitrogen TrueTag Donor DNA Kit, GFP, provides a complete and rapid solution to produce transfection-ready donor DNA to drive high-efficiency gene tagging in genome editing experiments. Benefits of the TrueTag Donor DNA kits include:
• Simple PCR amplification to add homology arms to the kit-provided donor template
• Reduces time needed for cloning of donor templates from days to hours
• Same-day transfection-ready donor DNA
• Obtain up to 100% edited cells
• Works with CRISPR, TALENs, and zinc-finger nucleases

The TrueTag Donor DNA Kit, GFP, provides everything you need to produce a high-quality donor DNA to tag your gene with GFP—simply provide two oligos to target the gene. This comprehensive yet flexible kit enables tagging of the carboxy- (C-) or amino- (N-) terminus of the gene with a choice of puromycin or blasticidin resistance cassettes to drive up to 100% edited cells. Validated with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNAs, the TrueTag Donor DNA kits are compatible with current genome editing technologies, including CRISPR, TALENs, and zinc-finger nucleases.

Each TrueTag Donor DNA Kit includes:
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

CTS™ PSC Cryopreservation Kit (Gibco™)

The CTS PSC Cryopreservation Kit is cGMP manufactured and used for the cryopreservation and recovery of pluripotent stem cells (PSCs). The kit consists of CTS PSC Cryomedium, a xeno-free solution for the cryopreservation of early passage PSCs, and CTS RevitaCell Supplement (100X), a chemically defined recovery supplement for use in the post-thaw culture medium. In combination, these reagents help minimize loss of cell viability, maximize post-thaw recovery, and minimize unwanted differentiation of pluripotent stem cells.

The CTS PSC Cryopreservation Kit:
• Provides efficient recovery and higher viability than traditional methods of PSC cryopreservation
• Facilitates regulatory filings through cGMP manufacturing and the availability of documentation
• Simplifies the transition from bench to clinic through the direct replacement of the RUO PSC Cryopreservation Kit

Efficient recovery
The CTS PSC Cryopreservation Kit achieves higher viability than traditional methods of cryopreservation. It is compatible with clump and single cell passaged PSCs cultured in a variety of media systems. CTS PSC Cryomedium is provided as a ready-to-use, complete solution containing 10% DMSO for efficient cell addition. Separate additions of enhancing reagents are not required, helping save valuable preparation time.

Facilitates regulatory filings
The CTS PSC Cryopreservation Kit is manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing site is ISO 13485-certified. Full documentation traceability and convenient access to our Drug Master File (DMF) are available for the CTS PSC Cryopreservation Kit.

Simplifies transition from bench to clinic
The CTS PSC Cryopreservation Kit provides the same efficient recovery, consistency, and reduced variability as the RUO PSC Cryopreservation Kit. With its additional safety testing, including adventitious agents, the CTS PSC Cryopreservation Kit enables a smooth transition from the RUO PSC Cryopreservation Kit.

CD80 Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse CD80 Simplex ProcartaPlex kit measures CD80 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: mouse
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: Serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

TrueTag™ Donor DNA Kit, RFP (Invitrogen™)

The Invitrogen TrueTag Donor DNA Kit, RFP, provides a complete and rapid solution to produce transfection-ready donor DNA to drive high-efficiency gene tagging in genome editing experiments. Benefits of the TrueTag Donor DNA kits include:
• Simple PCR amplification to add homology arms to the kit-provided donor template
• Reduces time needed for cloning of donor templates from days to hours
• Same-day transfection-ready donor DNA
• Obtain up to 100% edited cells
• Works with CRISPR, TALENs, and zinc-finger nucleases

The TrueTag Donor DNA Kit, RFP, provides everything you need to produce a high-quality donor DNA to tag your gene with RFP—simply provide two oligos to target the gene. This comprehensive yet flexible kit enables tagging of the carboxy- (C-) or amino- (N-) terminus of the gene with a choice of puromycin or blasticidin resistance cassettes to drive up to 100% edited cells. Validated with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNAs, the TrueTag Donor DNA kits are compatible with current genome editing technologies, including CRISPR, TALENs, and zinc-finger nucleases.

Each TrueTag Donor DNA Kit includes:
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

CytoVista™ 3D Cell Culture Clearing Reagent (Invitrogen™)

CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm. The reagent can also be used as an imaging solution with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology or brightness, or specificity of the fluorophores.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

ViewRNA™ Tissue Assay Blue Module (Invitrogen™)

This VewRNA Tissue Assay Blue Module is designed for use with the ViewRNA Tissue Assay Core Kit. The Core Kit, when combined with a ViewRNA Tissue probe set (purchased separately), enables the detection of a single mRNA target in tissue sections using the Fast Red substrate. The ViewRNA Tissue Assay Blue Module enables the detection of a second mRNA target using the Fast Blue substrate and must be used in combination with the Core Kit and two probes sets in order to run a 2-plex assay. Each kit contains sufficient reagents to process a minimum of six slides at a time. ViewRNA TYPE 1 probe sets are for use with the Fast Red substrate (Core Kit), while ViewRNA TYPE 6 probe sets are for use with the Fast Blue substrate (Blue Module).

Learn more about and order ViewRNA Tissue probe sets ›

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) tissue or OCT (optimal cutting temperature) embedded frozen tissue sections. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

KingFisher™ Duo-Ready DNA Ultra 2.0 Prefilled Plates (Thermo Scientific™)

KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Duo and KingFisher Duo Prime purification systems. KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates enable isolation of genomic DNA from 50 to 400 µL of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.

Key features of the KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates:
• Maximize convenience with MagMAX DNA Multi-Sample Ultra 2.0 isolation chemistry prefilled into KingFisher SBS microtiter plates
• Increase ease of use—process 12 samples on instrument in 45 minutes without the hassle of reagent setup
• Designed to reduce user error
• Helps to reduce hands-on time
• Formatted specifically for KingFisher Duo instruments

All the benefits of MagMAX sample prep chemistries in automation-ready plate format
Maximize convenience and confidence with ready-to-use prefilled plates and accessory plastics configured specifically for the KingFisher Duo Prime instrument. KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates eliminate the need to aliquot magnetic beads, lysis solutions, and wash solutions. Just peel off the foil seal from the KingFisher Duo-Ready DNA Ultra 2.0 prefilled plate; add enhancer, 50-400 µL of your sample, and proteinase K; and load the plate into the KingFisher Duo Prime instrument.

Eliminate the need for pipetting reagents, searching for plastics, or risking reagent mix-ups in your DNA isolation workflow.

Complete, prefilled automation-ready sample prep kit improves convenience with all accessory plastics
Not only are the KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates prefilled with industry–leading MagMAX DNA Multi-Sample Ultra 2.0 reagents (Cat. No. A36570), the plates come with all required tip-combs and plastics necessary for processing 96 samples in one small convenient box.

What you get
• Eight foil-sealed KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates sufficient to process 12 samples in each instrument run (95 samples in total)
• Eight complete sets of accessory plastics (KingFisher 12-tip comb, elution strips, and caps)
• Proteinase K solution, enhancer solution, and elution solution sufficient for 96 samples

Apoptosis 6-Plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Apoptotic 6-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 6 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell lysate
Sample volume: cell lysate: 50 µL
Reported application: multiplex immunoassay
Target list: Bcl-2; p53; cleaved PARP; caspase-3 (active); cytochrome c; GAPDH
Bead regions: Bcl-2: 57; p53: 52; cleaved PARP: 28; caspase-3 (active): 44; cytochrome c: 54; GAPDH: 12
Assay range (as determined for Lot 1): Bcl-2: 35.7-26000 pg/mL; p53: 57.1-41600 pg/mL; cleaved PARP: 0.3-250 pg/mL; caspase-3 (active): 1.2-900 pg/mL; cytochrome c: 83.1-60600 pg/mL; GAPDH: 186.6-136000 pg/mL
Assay sensitivity (as determined for Lot 1): Bcl-2: 6.37 pg/mL; p53: 10.01 pg/mL; cleaved PARP: 0.12 pg/mL; caspase-3 (active): 0.57 pg/mL; cytochrome c: 34.02 pg/mL; GAPDH: 48.54 pg/mL

PD-1 Mouse ProcartaPlex™ Simplex Kit (Invitrogen™)

The Mouse PD-1 Simplex ProcartaPlex kit measures PD-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: mouse
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: Serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

CytoVista™ Sagittal Mouse Brain Slicer, 1 mm (Invitrogen™)

This 1-mm sagittal mouse brain slicer allows you to easily cut a mouse brain into smaller pieces for easier imaging with objectives that have a limited working distance. This slicer is 3D-printed out of resin and can be easily washed and cleaned between uses. All you need is a standard razor blade to start slicing your tissues.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

TrueTag™ Donor DNA Kit, luciferase (Invitrogen™)

The Invitrogen TrueTag Donor DNA Kit, luciferase, provides a complete and rapid solution to produce transfection-ready donor DNA to drive high-efficiency gene tagging in genome editing experiments. Benefits of the TrueTag Donor DNA kits include:
• Simple PCR amplification to add homology arms to the kit-provided donor template
• Reduces time needed for cloning of donor templates from days to hours
• Same-day transfection-ready donor DNA
• Obtain up to 100% edited cells
• Works with CRISPR, TALENs, and zinc-finger nucleases

The TrueTag Donor DNA Kit, luciferase, provides everything you need to produce a high-quality donor DNA to tag your gene with luciferase—simply provide two oligos to target the gene. This comprehensive yet flexible kit enables tagging of the carboxy- (C-) or amino- (N-) terminus of the gene with a choice of puromycin or blasticidin resistance cassettes to drive up to 100% edited cells. Validated with our TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNAs, the TrueTag Donor DNA kits are compatible with current genome editing technologies, including CRISPR, TALENs, and zinc-finger nucleases.

Each TrueTag Donor DNA Kit includes:
• Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human beta-actin (ACTB)

Image-iT™ Plant Tissue Clearing Reagent (Invitrogen™)

Image-iT Plant Tissue Clearing Reagent is used to render whole-plant tissue optically clear for imaging. Image-iT Plant Tissue Hard-Set Mountant can then be used to mount and preserve the optically cleared whole-plant tissue for imaging, storage, and archiving. These reagents are compatible with both fluorescence-based and colorimetric-based applications. Image-iT Plant Tissue Clearing Reagent is an effective replacement for chloral hydrate, traditionally used to clear plant tissues in botanical microscopy.

Features of Image-iT Plant Tissue Clearing Reagent include:
• Clears plant tissue for whole-specimen imaging
• Soft-set mountant for imaging with refractive index of 1.45
• Effective replacement for chloral hydrate

Plant tissues often have structures with different refractive indexes, causing light scattering and spherical aberration, resulting in opaqueness. Image-iT Plant Tissue Clearing Reagent, with a high refractive index of 1.45, allows light to pass through the plant tissues with minimal light scattering, resulting in optical clarity and the ability to image deeper within the tissue.

Traditionally, researchers used chloral hydrate to clear plant specimens, but under current regulations it is a Federally Regulated Schedule IV substance (1), making it harder to acquire and use. Image-iT Plant Tissue Clearing Reagent is an effective replacement for chloral hydrate in almost all applications and tissue types where optical clearing is required.

Image-iT Plant Tissue Clearing Reagent and Image-iT Plant Tissue Hard-Set Mountant are based on Visikol™ for Plant Biology™ and Visikol™ Mount. Visikol for Plant Biology has been used in various fluorescent and colorimetric studies with plants (2,3,4) and has proved an effective substitute for chloral hydrate (1,5).

References:
1. Villani TS, Koroch AR, Simon JE. An improved clearing and mounting solution to replace chloral hydrate in microscopic applications. Appl Plant Sci. 2013 1(5).
2. Aoki Y, Van Trung N, Suzuki S. Impact of Piper betle leaf extract on grape downy mildew: effects of combining 4-allylpyrocatechol with eugenol, α-pinene or β-pinene. Plant Protect Sci. 2018 55: 23-30.
3. Lee KJI, Calder GM, Hindle CR, Newman JL, Robinson SN, Avondo JJHY, Coen ES. Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity. J Exp Bot. 2017 68(3):527-538.
4. Vergara-Díaz O, Chairi F, Vicente R, Fernandez-Gallego JA, Nieto-Taladriz MT, Aparicio N, Kefauver SC, Araus JL. Leaf dorsoventrality as a paramount factor determining spectral performance in field-grown wheat under contrasting water regimes. J Exp Bot. 2018 69(12):3081-3094.
5. Urban MA, Barclay RS, Sivaguru M, Punyasena SW. Cuticle and subsurface ornamentation of intact plant leaf epidermis under confocal and superresolution microscopy. Microsc Res Tech. 2018 81(2):129-140.

KingFisher™ Flex-Ready DNA Ultra 2.0 Prefilled Plates (Thermo Scientific™)

KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Flex purification systems. KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates enable isolation of genomic DNA from 50 to 400 µL of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.

Key features of the KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates:
• Maximize convenience with MagMAX DNA Multi-Sample Ultra 2.0 isolation chemistry prefilled into KingFisher SBS microtiter plates
• Increase ease of use—process 96 samples on instrument without the hassle of reagent setup
• Designed to reduce user error
• Helps to reduce hands-on time
• Formatted specifically for KingFisher Flex instruments

All the benefits of MagMAX sample prep chemistries in automation-ready plate format
Maximize convenience and confidence with ready-to-use prefilled plates and accessory plastics configured specifically for the KingFisher Flex instrument. KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates eliminate the need to aliquot magnetic beads, lysis solutions, and wash solutions. Just peel off the foil seals from the KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates; add enhancer, 50-400 µL of your sample, and proteinase K; and load all of the plates into the KingFisher Flex instrument.

Eliminate the need for pipetting reagents, searching for plastics, or risking reagent mix-ups in your DNA isolation workflow.

Complete, prefilled automation-ready sample prep kit improves convenience with all accessory plastics
Not only are KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates prefilled with industry-leading MagMAX DNA Multi-Sample Ultra 2.0 reagents (Cat. No. A36570), the plates come complete with all required tip-combs and plastics necessary for processing 96 samples in one small convenient box.

What you get
• Seven foil-sealed KingFisher Flex-Ready DNA Ultra 2.0 Prefilled Plates sufficient to process 96 samples in one instrument run (96 samples in total)
• One empty and sealed sample input 96 deep well plate
• KingFisher 96-tip comb
• Proteinase K solution and enhancer solution sufficient for 96 samples