Master Mixes for In-house Assay Development

TaqMan™ Environmental Master Mix 2.0 (Applied Biosystems™)

Applied Biosystems® TaqMan® Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.

- Tailored for quantitative, real-time PCR experiments
- Unrivalled sensitivity for challenging applications, low copy number target detection and multiplex PCR
- Includes pathogen detection and viral⁄bacterial load quantification
- Sensitive detection for reliable quantification of abundant and limited genetic targets
- Stable mix for high-throughput handling

Optimized Formulation for Unrivalled Performance

TaqMan® Environmental Master Mix 2.0 is a convenient 2X mix for target quantification that includes:

- AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure), a highly purified DNA Polymerase for improved detection of bacterial targets. This hot-start enzyme is inactive at room temperature so reactions can be set up on the benchtop. Enzyme is activated during thermal cycling.
- Passive internal reference based on proprietary ROX™ dye for precise data analysis.

RNA UltraSense™ One-Step Quantitative RT-PCR System (Applied Biosystems™)

The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript® III RT and Platinum® Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/µl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:

• SuperScript® III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
• Platinum® Taq DNA Polymerase with hot-start technology for improved specificity
• Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
• Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
• Validated performance on multiple real-time instrument platforms
• One-step format for speed, convenience, and less reaction-to-reaction variability

Real-time quantitative amplification of RNA, including:
• RNA viruses
• Low-abundance RNA transcripts
• Difficult-to-amplify RNA targets
• High-value RNA detection and research