MicroSEQ & RapidFinder STEC PCR Assays

MicroSEQ™ E. coli O157:H7 Detection Kit (Applied Biosystems™)

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample
• Genome sequencing using the Applied Biosystems SOLiD™ or 5500 Series Genetic Analyzer platforms helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control
Validated by the AOAC Research Institute and AFNOR according to ISO 16140

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment

Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment

Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Listeria spp. Detection Kit (Applied Biosystems™)

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total)
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control
Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC helps eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

High Specificity
The specificity of the MicroSEQ® Listeria spp. Detection Kit has been improved through the use of TaqMan® chemistry. Unlike SYBR® Green chemistry, which has only target-specific primers, TaqMan® chemistry has both primers and probes that are specific to the target, an additional way to help prevent false positives.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The MicroSEQ® Listeria spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ E. coli O157:H7 Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25 g sample
• Genome sequencing using the Applied Biosystems SOLiD™ platform helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control
Validated by the AOAC Research Institute and AFNOR according to ISO 16140

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment
Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment
Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC and AFNOR Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute, and has been validated by AFNOR according to ISO16140.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

RapidFinder™ STEC Screening Assay (Applied Biosystems™)

The Applied Biosystems™ RapidFinder™ STEC Screening Assay is part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E. coli (STEC) O157:H7 and Big 6 non‐O157:H7 serogroup (O26, O45, O103, O111, O121 and O145) in a variety of beef products. The RapidFinder STEC Screening Assay was co-developed with the USDA-ARS and contains 96 complete qPCR assays targeted to screen for Shiga toxin 1 and 2 (stx1/stx2), eae, and potential O157:H7 presence. The assay is designed to be inclusive of all know allelic variants of stx and eae, including stx2g and stx2f. All reactions include an internal positive control (IPC) and ROX™ passive reference. Samples assessed as negative require no further testing. Samples assessed as positive must be further tested using the RapidFinder™ STEC Confirmation Assay.

The RapidFinder STEC Detection Workflow provides:
• Greater confidence in results—part of a validated workflow for detection of as little as 1-5 CFU of pathogenic STEC in your sample
• Simplified workflow—simultaneous detection of E. coli O157:H7 and the Big 6 non-O157:H7 strains in 375 g beef samples with a closed-tube, single-step lyophilized detection format that fits within your existing testing program
• Reduced chance of false results—through use of high-quality DNA preparation, proprietary assay design, and internal positive control
• Same day detection of pathogenic STECs and clearance of STEC-negative beef samples in as little as 12 hours, supporting early product release
• Minimized costs and laboratory space with the use a lower volume of a nonproprietary single-step enrichment medium, eliminating the need to purchase proprietary media
• Compatibility with RapidFinder™ Express software v.1.2 and later—enables easy, automated presence/absence detection and interpretation of results

Studies have shown that the RapidFinder STEC assays have a high level of sensitivity and specificity for E. coli O157:H7 and the Big 6 non-O157 STEC strains*. They detected all inclusion strains and showed no cross-reactivity to any of the exclusion strains tested. The stx assays detected all known variants of stx1 and stx2, including stx2f and stx2g. The optimized workflow co-developed with USDA-ARS showed equivalent detection to the MLG reference method, and the internal positive control in both assays significantly reduces the risk of false-negatives.

Applications
The RapidFinder STEC Detection Workflow is intended for use by microbiological analysts who need to test for STEC in beef samples. Combined with the Applied Biosystems 7500 Fast RT-PCR System, RapidFinder STEC screening and confirmation assays provide a comprehensive solution for rapid detection, identification, and confirmation of Big 6 STECs and E. coli 0157:H7 to meet importation and exportation requirements for the beef industry. Specific workflows are detailed in the figures below and include both automated and spin column-based DNA isolation and real-time PCR detection of E. coli O157:H7 and Big 6 non-O157 strains in beef samples.

RapidFinder Express Software
When integrated into the RapidFinder STEC Detection Workflow, RapidFinder Express Software automates data interpretation and generates user instructions on how to proceed with a sample. Data displays are available for novice and advanced users, showing simple presence/absence calls or raw data files using 7500 Fast System SDS Software.

RapidFinder STEC Screening and Confirmation Assays meet USDA FSIS guidance
The RapidFinder STEC Detection Workflow, a two-stage real-time PCR method, is designed to accurately detect, identify, and confirm E. coli O157:H7 and the Big 6 serogroups of non-O157 Shiga toxin-producing E. coli defined by the USDA Food Safety and Inspection Service (FSIS) as adulterants in the American beef industry.

Learn more about the RapidFinder STEC Detection Workflow >

*Swimley, Michelle S. Design and Evaluation of Two-Stage Multiplex Real-Time PCR Method for Detecting O157:H7 and non-O157 STEC Strains from Beef Samples. IAFP 2015.

MicroSEQ™ E. coli O157:H7 Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time-to-results in as little as 8 hours for a 25g sample
• Genome sequencing using the Applied Biosystems SOLiD™ platform helps ensure high specificity
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® E. coli O157:H7 Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Enhanced Specificity
To design an assay highly specific for E. coli O157:H7, the genome of E. coli O55:H7 was sequenced using the next-generation Applied Biosystems® SOLiD™ platform and compared to the publicly available sequence of E. coli O157:H7. Sequences specific to E. coli O157:H7 were then used as targets to design the MicroSEQ® E. coli O157:H7 Detection Kit. The MicroSEQ® E. coli O157:H7 Detection Kit owes its additional specificity to the use of TaqMan® chemistry. Unlike SYBR® Green chemistry that uses only target-specific primers, TaqMan® chemistry uses both primers and probes that are specific to the target to help prevent false positives.

Workflow Flexibility
To better meet the needs of food testing laboratories, the MicroSEQ® E. coli O157:H7 Detection Kit offers the flexibility of three different workflows.

Fast Time to Results
• 8-10 hour time-to-results
• 25 g sample
• BHI enrichment
Standard Media
• 18 hour time to results
• 25 g sample
• BPW enrichment
Larger Volumes
• 18 hour time to results
• 375 g sample of ground beef or beef trim
• BPW enrichment

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® E. coli O157:H7 Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested MethodsSM certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® E. coli O157:H7 Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® E. coli O157:H7 Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make E. coli O15:H7 detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Salmonella spp. Detection Kit (Applied Biosystems™)

This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ® Salmonella spp. Detection Kit detects Salmonella spp. simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 19 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute and AFNOR according to ISO 16140


Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Salmonella spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC and AFNOR Certified
The full workflow including the MicroSEQ® Salmonella spp. Detection Kit, PrepSEQ sample preparation kits, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested Methods certification from the AOAC Research Institute, and AFNOR validation status.

Optimized Sample Preparation
The MicroSEQ® Salmonella spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ Salmonella spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Listeria monocytogenes simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with the PrepSEQ® Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ® Listeria monocytogenes Detection Kit with a 100 reaction PrepSEQ™ Nucleic Acid Extraction Kit and offer it here as a single product.

Features of the MicroSEQ® Listeria monocytogenes Detection Starter Kit:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 27 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ® pathogen detection kits
• Validated by the AOAC Research Institute

Optimized Sample Preparation
The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ® Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Universal Fast PCR Cycling Conditions
All MicroSEQ® pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.

Clear Results using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow, including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ® Nucleic Acid Extraction Kit, 7500 Fast instrument and RapidFinder™ Express software, has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Listeria monocytogenes detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria monocytogenes detection as fast, easy, and reliable as possible.

For testing of Food and Environmental samples only.

MicroSEQ™ Listeria spp. Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total)
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC helps eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

High Specificity
The specificity of the MicroSEQ® Listeria spp. Detection Kit has been improved through the use of TaqMan® chemistry. Unlike SYBR® Green chemistry, which has only target-specific primers, TaqMan® chemistry has both primers and probes that are specific to the target, an additional way to help prevent false positives.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The MicroSEQ® Listeria spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

RapidFinder™ STEC Confirmation Assay (Applied Biosystems™)

The Applied Biosystems™ RapidFinder™ STEC Confirmation Assay is part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E. coli (STEC) O157:H7 and Big 6 non‐O157:H7 serogroup (O26, O45, O103, O111, O121, and O145) in a variety of beef products. Samples assessed as positive using the RapidFinder STEC Screening Assay are further tested using this RapidFinder STEC Confirmation Assay. All reactions include an internal positive control (IPC) and ROX™ passive reference.

The RapidFinder STEC Detection Workflow provides:
• Greater confidence in results—part of a validated workflow for detection of as little as 1-5 CFU of pathogenic STEC in your sample
• Simplified workflow—simultaneous detection of E. coli O157:H7 and the Big 6 non-O157:H7 strains in 375 g beef samples with a closed-tube, single-step lyophilized detection format that fits within your existing testing program
• Reduced chance of false results—through use of high-quality DNA preparation, proprietary assay design, and internal positive control
• Same day detection of pathogenic STECs and clearance of STEC-negative beef samples in as little as 12 hours, supporting early product release
• Minimized costs and laboratory space with the use a lower volume of a nonproprietary single-step enrichment medium, eliminating the need to purchase proprietary media
• Compatibility with RapidFinder™ Express software v.1.2 and later—enables easy, automated presence/absence detection and interpretation of results

Studies have shown that the RapidFinder STEC assays have a high level of sensitivity and specificity for E. coli O157:H7 and the Big 6 non-O157 STEC strains*. They detected all inclusion strains and showed no cross-reactivity to any of the exclusion strains tested. The stx assays detected all known variants of stx1 and stx2, including stx2f and stx2g. The optimized workflow co-developed with USDA-ARS showed equivalent detection to the MLG reference method, and the internal positive control in both assays significantly reduces the risk of false-negatives.

Applications
The RapidFinder STEC Detection Workflow is intended for use by microbiological analysts who need to test for STEC in beef samples. Combined with the Applied Biosystems 7500 Fast RT-PCR System, RapidFinder STEC screening and confirmation assays provide a comprehensive solution for rapid detection, identification, and confirmation of Big 6 STECs and E. coli 0157:H7 to meet importation and exportation requirements for the beef industry. Specific workflows are detailed in the figures below and include both automated and spin column-based DNA isolation and real-time PCR detection of E. coli O157:H7 and Big 6 non-O157 strains in beef samples.

RapidFinder Express Software
When integrated into the RapidFinder STEC Detection Workflow, RapidFinder Express Software automates data interpretation and generates user instructions on how to proceed with a sample. Data displays are available for novice and advanced users, showing simple presence/absence calls or raw data files using 7500 Fast System SDS Software.

RapidFinder STEC Screening and Confirmation Assays meet USDA FSIS guidance
The RapidFinder STEC Detection Workflow, a two-stage real-time PCR method, is designed to accurately detect, identify, and confirm E. coli O157:H7 and the Big 6 serogroups of non-O157 Shiga toxin-producing E. coli defined by the USDA Food Safety and Inspection Service (FSIS) as adulterants in the American beef industry.

Learn more about the RapidFinder STEC Detection Workflow >

*Swimley, Michelle S. Design and Evaluation of Two-Stage Multiplex Real-Time PCR Method for Detecting O157:H7 and non-O157 STEC Strains from Beef Samples. IAFP 2015.

MicroSEQ™ Listeria monocytogenes Detection Kit (Applied Biosystems™)

This kit is recommended for users who have already purchased the starter kit but would like to perform more tests. The MicroSEQ® Listeria monocytogenes Detection Kit detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Salmonella spp. Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit (Applied Biosystems™)

The MicroSEQ® Salmonella Species Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is recommended for those needing to prepare and analyze hundreds of food or environmental samples per day. The Starter Kit detects Salmonella species simply, reliably, and rapidly using the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The MicroSEQ® Salmonella Species Detection Kit is optimized for use with the PrepSEQ® Nucleic Acid Extraction Kit. For ease of ordering, we have combined a 96 reaction MicroSEQ® Salmonella Species Detection Kit with a 100 reaction PrepSEQ™ Nucleic Acid Extraction Kit and offer it here as a single product.

Features of the MicroSEQ® Salmonella Species Detection Starter Kit:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software-guided 3-step process
• Time-to-results of less than 3 hours after sample enrichment (less than 19 hours total)
• Eliminate false negatives with internal positive control
• Detect 1–3 colony-forming units (CFU) per 25 grams of food
• Universal PCR cycling conditions for all MicroSEQ® pathogen detection kits
• Validated by the AOAC Research Institute

Optimized Sample Preparation
The MicroSEQ® Salmonella Species Detection Kit is optimized for use with PrepSEQ® Nucleic Acid Extraction Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples for detection of 1–3 cfu per 25 grams of sample. In combination with the automated MagMAX™ Express-96 Deep Well Magnetic Particle Processor, this optimized solution enables laboratories to prepare a wide range of sample types at rates of up to hundreds of samples per eight-hour shift.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use and consistency of results, the reagents used in the MicroSEQ® Salmonella Species Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Universal Fast PCR Cycling Conditions
All MicroSEQ® pathogen detection kits utilize universal PCR cycling conditions which reduce error and allow maximum user flexibility. Fast PCR chemistries are completed in 40 minutes—saving time and clearing presumptive negatives quickly.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express Software (available separately) guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow including the MicroSEQ® Salmonella Species Detection Kit, PrepSEQ® Nucleic Acid Extraction Kit, 7500 Fast instrument and RapidFinder™ Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ® Salmonella Species Detection Starter Kit with PrepSEQ™ Nucleic Acid Extraction Kit is part of a complete food testing solution. The kit includes the reagents required to prepare up to 100 sample preparations and run 96 Salmonella species detection assays. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella species detection as fast, easy, and reliable as possible.

For testing of Food and Environmental samples only.

MicroSEQ™ Listeria spp Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food and environmental samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. This system comes with a PrepSEQ™ Nucleic Acid Extraction Kit.

Key product features:
• Enjoy ease-of-use from a streamlined, lyophilized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (27-31 hours total)
• Detect 1-3 colony-forming units (CFU) in 25 grams of food or in environmental samples
• Get optimal performance on a wide range of food sample and environmental surface types
• Eliminate false negatives with internal positive control

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC helps eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

High Specificity
The specificity of the MicroSEQ® Listeria spp. Detection Kit has been improved through the use of TaqMan® chemistry. Unlike SYBR® Green chemistry, which has only target-specific primers, TaqMan® chemistry has both primers and probes that are specific to the target, an additional way to help prevent false positives.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The MicroSEQ® Listeria spp. Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of sample types for detection of 1-3 CFU per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder™ Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria spp. Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Listeria monocytogenes Detection Starter Kit, with PrepSEQ™ Rapid Spin Sample Preparation Kit (Applied Biosystems™)

The MicroSEQ® Listeria monocytogenes Detection Kit Starter Kit is recommended for first time users in laboratories that routinely prepare up to 100 samples a day. It detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.
• Enjoy ease-of-use from a streamlined, lypholized format and a simple, software guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 27 hours total)
• Detect 1-3 colony-forming units (CFU) per 25 grams of food
• PrepSEQ™ Rapid Spin Sample Preparation Kit included
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with internal positive control
• Validated by the AOAC Research Institute

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ® Listeria monocytogenes Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, the target-specific primer and probe set, Internal Positive Control (IPC) and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Simple, Three-Step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps.
1. Enrich sample
2. Prepare DNA
3. Run test

AOAC Certified
The full workflow including the MicroSEQ® Listeria monocytogenes Detection Kit, PrepSEQ™ sample preparation kits, 7500 Fast instrument and RapidFinder Express software has earned the Performance Tested Methods certification from the AOAC Research Institute.

Optimized Sample Preparation
The MicroSEQ® Listeria monocytogenes Detection Kit is optimized for use with PrepSEQ™ Sample Preparation Kits. PrepSEQ™ kits reduce pre-enrichment time, enhance assay performance and enable you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 cfu per 25 grams of sample.

Clear Results using a Software-Guided Procedure
After sample enrichment and preparation, RapidFinder® Express software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection and analysis are fully automated. When the assay is complete, the Software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications and prompts that enable you to quickly interpret the data and take appropriate action.

A Complete Solution
The MicroSEQ® Listeria monocytogenes Detection Kit is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Listeria detection as fast, easy and reliable as possible.

For Research Use Only. Not for use in diagnostics procedures.

MicroSEQ™ Salmonella spp. Detection Starter Kit with PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean (Applied Biosystems™)

The MicroSEQ® Salmonella spp. Detection Kit Starter Kit with PrepSEQ™ RapidSpin Sample Preparation Kit – Extra Clean enables the detection of Salmonella spp. simply, reliably, and rapidly in food samples using the Applied Biosystems® 7500 Fast Real-Time PCR System. It is recommended for first time users in laboratories that routinely prepare up to 100 samples a day and prefer a spin column-based workflow. This starter kit combines the PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean with a MicroSEQ Salmonella spp. Detection Kit for ease of ordering. The benefits of this workflow include:

• Enjoy the ease-of-use of a streamlined, lypholized format and simple, software-guided 3-step process
• Get your results quickly with time to results of less than 3 hours after sample enrichment (less than 19 hours total)
• Detect 1-3 colony-forming units (CFUs) per 25 grams of food
• Get optimal performance on a wide range of food sample types
• Eliminate false negatives with an internal positive control
• Validated by the AOAC Research Institute

Simple, Three-step Procedure
There is minimal training or prior experience needed for you to perform the assay in these three simple steps:
1. Enrich sample
2. Prepare DNA
3. Run test

Optimized Sample Preparation
The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is optimized for use with the MicroSEQ Salmonella spp. Detection Kit. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean reduces pre-enrichment time, enhances assay performance, and enables you to enrich and prepare high quality samples from a broad range of food sample types for detection of 1-3 CFUs per 25 grams of sample. The PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean includes extra tubes for a transfer step, which is recommended for foods with a high lipid content, such as infant formula and chicken wing samples. However, simpler matrices can also be prepared with this kit by skipping the transfer step.

Lyophilized for Efficiency and Ease of Use
For maximum ease of use, and consistency of results, the reagents used in the MicroSEQ Salmonella spp. Detection Kit are lyophilized into preformatted assay beads. The beads hold the active enzyme, target-specific primer and probe set, Internal Positive Control (IPC), and other reagents for PCR. The IPC is provided to help eliminate false negatives by detecting the presence of materials that can inhibit target amplification.

Clear Results Using a Software-guided Procedure
After sample enrichment and preparation, RapidFinder™ Express Software guides you through the entire workflow with on-screen instructions. Amplification, detection, data collection, and analysis are fully automated. When the assay is complete, the software presents an easy-to-read screen that allows you to view the results in each reaction tube. Results are clearly displayed and can be labeled with flags, notifications, and prompts that enable you to quickly interpret the data and take appropriate action.

AOAC Certified
The full workflow including the MicroSEQ Salmonella spp. Detection Kit, PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean, Applied Biosystems 7500 Fast Real-time PCR System, and RapidFinder Express Software has earned the Performance Tested Methods certification from the AOAC Research Institute.

A Complete Solution
The MicroSEQ Salmonella spp. Detection Kit with PrepSEQ RapidSpin Sample Preparation Kit – Extra Clean is part of a complete food testing solution. The kit includes everything required to run 96 reactions. All components have been designed for rapid implementation in food testing laboratories and performance-verified to make Salmonella detection as fast, easy and reliable as possible.