November 2018 New Products

EVOS™ 4X Objective, achromat, LWD, phase-contrast, 0.13NA/10.58WD (Invitrogen™)

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 4X
• Numerical Aperture: 0.13
• Working Distance: 10.58 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ 10X Objective, fluorite, LWD, phase-contrast, 0.30NA/7.13WD (Invitrogen™)

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.30
• Working Distance: 7.13 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

TNI Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human TNI Simplex ProcartaPlex kit measures TNI protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

EVOS™ 10X Objective, achromat, LWD, phase-contrast, 0.25NA/7.45WD (Invitrogen™)

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.25
• Working Distance: 7.45 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ 20X Objective, fluorite, LWD, phase-contrast, 0.45NA/6.12WD (Invitrogen™)

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 20X
• Numerical Aperture: 0.45
• Working Distance: 6.12 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

GeneMapper™ ID-X Software v1.6, full installation (Applied Biosystems™)

This product includes a single GeneMapper ID-X Software v1.6 full installation (including database) and license.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

CellEvent™ Senescence Green Flow Cytometry Assay Kit (Invitrogen™)

The CellEvent Senescence Green Flow Cytometry Assay Kit enables flow cytometric detection of cellular senescence via β-galactosidase hydrolysis. The kit contains an optimized buffer and a fluorescein-based reagent that contains two galactoside moieties, making it a specific target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzyme-cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

CellEvent Senescence Green Flow Cytometry Assay Kit features include:
• Fluorescent detection of β-galactosidase hydrolysis by flow cytometry
• Fixed cell assay
• Multi-color compatibility

When normal cells reach the end of their limited replicative lifespan, commonly referred to as the Hayflick limit, they enter into a senescent phase where they remain metabolically active without undergoing cell death processes. These senescent cells adopt a specific phenotypic state that includes the appearance of multi-nucleated cells, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological changes where cells become enlarged and extended. Through a variety of mechanisms, they may play a role in tumor suppression, tumor progression, aging, and tissue repair.

β-galactosidase resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The activity is optimal under lysosomal pH 4, but conventional assays measure at pH 6. It has been shown that normalized β-galactosidase activity is twice as high in senescent cells as in presenescent cells regardless of the pH value used for testing.

Traditionally, the colourimetric substrate for β-galactosidase, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (x-gal), has long been used to detect metabolic activity in cells in vitro. Upon hydrolysis by β-galactosidase, x-gal converts to a blue precipitate, which is not suitable for flow cytometric assays. Therefore, we have developed a sensitive, fluorescent substrate for β-galactosidase that can be used in flow cytometry assays for the detection of senescent cells.

GeneMapper™ ID-X Software v1.6, full upgrade (Applied Biosystems™)

This product includes a GeneMapper ID-X v1.6 full upgrade for previous full installation versions of GeneMapper ID-X Software. A prior registration code is required along with a new registration code.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

Cardiac Disease 9-Plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Cardiac Disease 9-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 9 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: NTproBNP; CTF-1; GDF-15; myoglobin; LDH-B; FABP-3; Big ET-1; TNI; CKMB
Bead regions: NTproBNP: 48; CTF-1: 12; GDF-15: 33; myoglobin: 47; LDH-B: 42; FABP-3: 43; Big ET-1: 61; TNI: 54; CKMB: 63
Assay range (as determine for Lot 1): NTproBNP: 3.6-14900 pg/mL; CTF-1: 11.4-46500 pg/mL; GDF-15: 12.2-50000 pg/mL; myoglobin: 652.6-2711600 pg/mL; LDH-B: 662-2711600 pg/mL; FABP-3: 146.5-600000 pg/mL; Big ET-1: 0.5-2050 pg/mL; TNI: 10.1-41700 pg/mL; CKMB: 2.7-11300 pg/mL
Assay sensitivity (as determine for Lot 1): NTproBNP: 11.1 pg/mL; CTF-1: 8.6 pg/mL; GDF-15: 9.7 pg/mL; Myoglobin: 635.7 pg/mL; LDH-B: 1775 pg/mL, FABP-3: 551.8 pg/mL, Big ET-1: 0.48 pg/mL; TNI: 9.7 pg/mL; CKMB: 0.7 pg/mL

EVOS™ 20X Objective, achromat, LWD, phase-contrast, 0.40NA/6.92WD (Invitrogen™)

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 20X
• Numerical Aperture: 0.40
• Working Distance: 6.92 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ 40X Objective, fluorite, LWD, phase-contrast, 0.65NA/1.79WD (Invitrogen™)

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 40X
• Numerical Aperture: 0.65
• Working Distance: 1.79 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

GeneMapper™ ID-X Software v1.6, client installation (Applied Biosystems™)

This product includes a single GeneMapper ID-X Software v1.6 client installation (no database) and one license.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

GeneMapper™ ID-X Software v1.6, client upgrade (Applied Biosystems™)

This product includes a GeneMapper ID-X v1.6 client upgrade for previous client installation versions of GeneMapper ID-X Software. A prior registration code is required along with a new registration code.

GeneMapper ID-X Software is an automated genotyping solution for forensic casework and databasing data analysis, as well as for paternity data analysis. It supports a multi-user database configuration and provides a set of streamlined data analysis and sample review tools. It also offers a comprehensive quality value system that is designed to be optimized and validated for use in both expert system and traditional data analysis workflows. GeneMapper ID-X Software supports genotyping applications on the Applied Biosystems 310, 3100, 3130, 3130xl, 3500, 3500xl, 3730, and SeqStudio genetic analyzers. The release notes for this version provide information that should allow laboratories to decide whether internal validation testing is necessary for its system to comply with laboratory guidelines or regulations.

GeneMapper ID-X Software v1.6 features include:
• Supported operating systems: Windows® 7 Professional, 64-bit (Service Pack 1); Windows 10 Enterprise, 64-bit; Windows 10 Enterprise 2016 LTSB
• Support for Java version 8
• Latest oracle security patches
• Support for CODIS 3.2 Revision 16 and CODIS 3.3 specifications
• Internationalized with local language support for English and Chinese
• Enhanced command line interface functionality
• Enhanced user interfaces for better usability
• Enhanced printing
• View Table by Marker functionality in Report Manager and export as PDF
• Enhanced profile comparison
• Enhanced Security and Audit Module

 Overview of GeneMapper ID-X Software:
• Powerful, easy-to-use data analysis tool designed to increase lab productivity
• Comprehensive Expert System capability enables analysis of forensic databasing samples delivered by automated data assessment functionality and efficient manual review tools
• Complete Expert Assistant solution for analysis of forensic casework samples combines an efficient suite of manual review features with an integrated mixture analysis tool
• Quality control functionality allows rapid allele match comparisons and concordance searches within a data set
• Extensive security, auditing, and e-signature capabilities help protect data integrity and control technical records
• Multi-user database configuration stores your projects in a central location and facilitates information exchange
• Command Line Interface—offers the ability to access GeneMapper ID-X Software automatically (without human intervention) and helps streamline its integration with LIMS installations and other applications in an existing laboratory infrastructure

Automated data assessment
GeneMapper ID-X Software was designed to fulfill the requirements of both Expert System and Expert Assistant software. A combination of sophisticated automated data assessment processes and efficient manual review tools deliver comprehensive Expert System capability. This facilitates optimal interaction between the analyst and the data, helping to significantly reduce the amount of analysis time required for single source samples. Features such as the Analysis Requirements check, Allelic Ladder Quality Assessment, Improved Quality Value System, and Analysis Summary analyze samples and segregate them into pass or review categories based on your thresholds. Once segregated, you are enabled to easily conduct manual review of samples.

Despite its capabilities to automate and streamline the analysis of single source samples, an Expert System is unable to make the final analysis decision for most forensic casework samples and in particular for those containing mixtures. GeneMapper ID-X Software is designed to harness the automated, rule-based, subject-specific knowledge to act as an Expert Assistant to the forensic analyst, to help simplify much of the analysis process and empower the analyst to make key interpretation decisions. A mixture analysis tool determines the number of contributors to a mixture and helps efficiently manage the resulting possible genotypes. This enables the forensic analyst to interpret mixtures and generate accurate statistical calculations.

Manual tools help improve data review
Data analysis efficiencies offered by the automated data review processes have been further enhanced through the introduction of a suite of manual review tools. These developments focus on the plot window, enabling quick and easy access to all aspects of the data, thus helping to simplify the data review process. Colored marker header bars indicate which markers within a sample have failed to meet the user defined thresholds and the Process Quality Value (PQV) Details window details which flags have been fired and how much the observed value differs from the threshold. In addition, expanded labeling options are available to distinguish alleles from artifacts and reviewers can directly access raw data for further troubleshooting. Electronic/peer review of the data is enhanced with the review and manual acceptance of marker and sample level PQVs along with a detailed label edit table display.

For further troubleshooting, reviewers can also directly access raw data. The ability to review and manually accept markers and sample-level PQVs in a detailed label edit table display enhances electronic/peer data review.

A complete, verified system
GeneMapper ID-X Software is an integral component of the complete Thermo Fisher Scientific system of instruments, reagents, software, and services for human-identity applications. It has undergone extensive verification testing to help ensure robust design features, performance, and reproducibility. When you partner with Thermo Fisher Scientific, you gain access to one of the most extensive human identification resource networks in the industry. Our dedicated team of experienced forensic professionals—with extensive forensic product development experience—worked closely with the forensic community to develop GeneMapper ID-X Software. The software is part of a system that is supported by on-site technical assistance from skilled Field Applications Support Scientists and Field Service Engineers.

EVOS™ Universal Stage Plate (Invitrogen™)

This EVOS Universal Stage Plate is designed to make a quick check of samples fast and easy. With a 1.5-inch diameter opening, it enables you to quickly observe cell staining, GFP expression, cell morphology, or confluence by simply moving the sample vessel across the stage. No additional vessel holders or accessories are needed. When more precise and secure alignment of your sample is desired, use a vessel holder from our wide range of holders for microscope slides, cell culture flasks, Petri dishes, microwell plates, and more that are accommodated on the EVOS microscope in conjunction with a stage plate such as Cat. Nos. AMEPVH089 or AMEPVH054.

The EVOS Universal Stage Plate offers these advantages:
• Exceptional flexibility
• Quick imaging by simply moving the vessel across the stage
• Compatible vessel holder adapters for a range of vessels holders

Explore the entire EVOS line of imaging systems and accessories ›
For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates ’

EVOS™ 40X Objective, achromat, LWD, phase-contrast, 0.65NA/2.74WD (Invitrogen™)

This achromat objective is ideal for general applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. This is also a phase-contrast objective, making it useful for hard-to-see, translucent speciments. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 40X
• Numerical Aperture: 0.65
• Working Distance: 2.74 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories