October 2018 New Products

Applied Biosystems™ RapidHIT™ ID ACE GlobalFiler™ Express 150 Sample Complete Kit (Applied Biosystems™)

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

The Applied Biosystems RapidHIT ID ACE GlobalFiler Express 150 Sample Complete Kit provides 150 self-contained, easy-to-use sample cartridges and a primary cartridge that contains all necessary reagents for sample preparation (including GlobalFiler Express chemistry) and capillary electrophoresis for 150 runs. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and in 90 minutes receive the lab-quality forensic DNA profile.

Features of the kit include:
• 1-minute hands-on-time workflow enabled by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 150 RapidHIT ID ACE GlobalFiler Express sample cartridges
• 6 positive control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 6 negative control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 1 primary cartridge - GlobalFiler Express 150
• 1 gel cartridge
• 1 utility cartridge
• 1 ACE GlobalFiler Express Control Cartridge (for install)
• 1 positive control RapidHIT ID ACE GlobalFiler Express sample cartridge (for install)

SureQuant™ AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of key phosphorylation events of AKT signaling pathway proteins using mass spectrometry (MS).

The SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation) consists of two modules:
SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module includes reagents necessary to immuno-enrich phosphorylated AKT-mTOR pathway proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant AKT Pathway (Phospho) Absolute Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide and LightPeptide mixtures for absolute peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant AKT Pathway (Phospho) Multiplex Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate phosphorylation of ten key proteins in the AKT signaling pathway
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key phosphorylated proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin, and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant AKT Pathway (Phospho) Absolute Quantitation Module provides reagents for absolute quantitation of 30 unique peptides from 10 phosphorylated AKT-mTOR signaling pathway target proteins using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the phosphorylated target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

Applied Biosystems™ RapidHIT™ ID System (Applied Biosystems™)

The Applied Biosystems RapidHIT ID System is a fast and simple-to-use instrument that produces trusted lab-quality forensic DNA profiles in 90 minutes. The system integrates sample preparation and capillary electrophoresis to generate DNA profiles that are aggregated within Applied Biosystems RapidLINK Software for direct upload to the database of choice or for further review and analysis.

The RapidHIT ID System delivers the reliable, reproducible, high-quality data you have come to expect from the Applied Biosystems brand. This system is for use with Applied Biosystems RapidLINK Software v1.0 and its v1.0 applications.

Features of the Applied Biosystems RapidHIT ID System include:
• Fast 90-minute system run time
• 1-minute hands-on time with integrated sample cartridge
• Consumables tracking through radio frequency identification (RFID)
• Intuitive touchscreen interface
• Facial recognition and barcode camera
• Fingerprint reader
• Up to six months of on-instrument time for the primary cartridge with refrigeration mechanism
• Expert system-capable
• Sample/data accessioning workflow-capable

SureQuant™ AKT Isoform Absolute Quantitation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Isoform Absolute Quantitation Module provides a complete quantitation solution for the analysis of key AKT expression using mass spectrometry (MS).

SureQuant AKT Isoform Absolute Quantitation Module features include:
Complete—contains validated AQUA HeavyPeptide and LightPeptide mixtures, diluent, matrix, system suitability standard including optimized acquisition and analysis methods
Validated—peptides rigorously tested for successful quantitation of each target peptide
Multiplex—able to quantitate AKT1, AKT2, and AKT3 isoforms and phosphorylation of AKT

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This kit is ideal for monitoring AKT expression levels of the three isoforms and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Isoform Absolute Quantitation Module provides reagents for absolute quantitation of six unique peptides from AKT using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

This kit can be used with SureQuant AKT Isoform IP and MS Sample Preparation Module for enriched multiplex sample preparation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of key phosphorylation events of AKT signaling pathway proteins using mass spectrometry (MS).

The SureQuant AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) consists of two modules:
SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module includes reagents necessary to immuno-enrich phosphorylated AKT-mTOR pathway proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant AKT Pathway (Phospho) Relative Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide mix for relative peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant AKT Pathway (Phospho) Multiplex Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate phosphorylation of ten key proteins in the AKT signaling pathway
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key phosphorylated proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin, and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant AKT Pathway (Phospho) Relative Quantitation Module provides reagents for relative quantitation of 30 unique peptides from 10 phosphorylated AKT-mTOR signaling pathway target proteins using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the phosphorylated target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ AKT Pathway Multiplex Panel (Relative Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway Multiplex Panel (Relative Quantitation) provides a complete quantitation solution for the enrichment and analysis of key AKT signaling pathway proteins using mass spectrometry (MS).

The SureQuant AKT Pathway Multiplex Panel (Relative Quantitation) consists of two modules:
SureQuant AKT Pathway IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich AKT-mTOR pathway proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant AKT Pathway Relative Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide mix for relative peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant AKT Pathway Multiplex Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful MS-based quantitation of each target peptide
Multiplex—able to quantitate ten key proteins in the AKT signaling pathway
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway activity.

The SureQuant AKT Pathway IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated-antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant AKT Pathway Relative Quantitation Module provides reagents for relative quantitation of 30 unique peptides from 10 AKT-mTOR signaling pathway target proteins using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

Applied Biosystems™ RapidLINK™ Software v1.0 License (Applied Biosystems™)

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

Applied Biosystems RapidLINK Software v1.0 is part of the Applied Biosystems RapidHIT ID System. This fast and simple system produces trusted lab-quality forensic DNA profiles in 90 minutes that are aggregated within software for direct upload to the database of choice or for further review and analysis. This data management software expedites identity confirmation securely and efficiently.

This product provides a single Applied Biosystems RapidLINK Software v1.0 serial number and a selection of quantities of user licenses.
Features of the Applied Biosystems RapidLINK Software v1.0 include:
• Securely manage one or more Applied Biosystems RapidHIT ID systems from a central location
• Review DNA profile results in real time prior to upload
• Enable or disable users in real time
• Collect and review metadata using built-in reporting tools
• Expert system-capable
• Sample/data accessioning workflow-capable

PRKCA (PKC alpha) Recombinant Human Protein (Invitrogen™)

The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

Applied Biosystems™ RapidLINK™ Software v1.0 Kinship Application (Applied Biosystems™)

The Applied Biosystems RapidLINK Kinship Application is an easy-to-use application software that confirms the relationship between two profiles on a three-generation family pedigree. The application generates a printable summary report of the likelihood ratio of the overall relationship and of the allelic relationship at each locus. For use only with Applied Biosystems RapidLink Software v1.0.

Features of the RapidLINK Kinship Application include:
• Confirmation of specific family relationship between two DNA profiles
• Flexible with regard to size of pool
• Reduced workload for lab personnel
• Calculates likelihood ratios of two DNA profiles on a family pedigree
• User-defined search stringency
• Automatically generated report

SureQuant™ RAS Isoform Panel (Absolute Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant RAS Isoform Panel (Absolute Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of NRAS, HRAS, and KRAS isoforms using mass spectrometry (MS).

The SureQuant RAS Isoform Panel (Absolute Quantitation) consists of two modules:
SureQuant RAS Isoform IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich NRAS, KRAS, and HRAS proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant RAS Isoform Absolute Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide and LightPeptide mixtures for absolute peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instrumentation and Nanospray LC instrumentation

SureQuant RAS Isoform Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate isoforms of RAS
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of the RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This kit is ideal for monitoring expression levels of the three isoforms in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant RAS Isoform IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant RAS Isoform Absolute Quantitation Module provides reagents for absolute quantitation of three unique peptides from NRAS, KRAS, and HRAS isoforms using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ AKT Pathway (Phospho) IP and MS Sample Preparation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation for studies of key phosphorylation events of the AKT signaling pathway using mass spectrometry (MS).

SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module features include:
• Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
• Validated—antibodies validated for specificity and background
• Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
• Multiplex—able to immuno-enrich phosphorylated AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for the multiplexed immuno-enrichment of key phosphorylated proteins in the AKT signaling pathway and has been optimized for use with the SureQuant quantitation modules.

The SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant AKT Pathway (Phospho) Absolute Quantitation Module or the SureQuant AKT Pathway (Phospho) Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the phosphorylated target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ AKT Pathway IP and MS Sample Preparation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of ten key AKT signaling pathway proteins for mass spectrometry (MS).

SureQuant AKT Pathway IP and MS Sample Preparation Module features include:
• Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
• Validated—antibodies validated for specificity and background
• Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
• Multiplex—able to immuno-enrich AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for the multiplexed immuno-enrichment of key proteins in the AKT signaling pathway and has been optimized for use with the SureQuant quantitation modules.

The SureQuant AKT Pathway IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant AKT Pathway Absolute Quantitation Module or the SureQuant AKT Pathway Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ TP53 Panel IP and MS Sample Preparation Module (Thermo Scientific™)

The Thermo Scientific SureQuant TP53 Panel IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of TP53 for mass spectrometry (MS).

SureQuant TP53 Isoform IP and MS Sample Preparation Module features include:
Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
Validated—antibodies validated for specificity and background
Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
Multiplex—able to immuno-enrich TP53 and phosphorylated TP53 protein

The tumor suppressor protein TP53 functions as a negative checkpoint regulator for abnormal cell signaling and can induce cell cycle arrest, activate DNA repair proteins, or initiate apoptosis. Mutations in TP53 often result abnormal cell growth and are commonly found in many types of cancer. This module is ideal for multiplexed immuno-enrichment of TP53 protein for use with the SureQuant quantitation modules.

The SureQuant TP53 Panel IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins and removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant TP53 Panel Absolute Quantitation Module or the SureQuant TP53 Panel Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

TREM-1 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human TREM-1 Simplex ProcartaPlex kit measures TREM-1 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

Applied Biosystems™ RapidLINK™ Software v1.0 Match Application (Applied Biosystems™)

The Applied Biosystems RapidLINK Match Application is an easy-to-use application software that provides automatic matching of DNA profiles in the RapidLINK database. The application compares one reviewed profile to all other available profiles in a passive search, so that user-defined parameters are automatically applied to all match queries. For DNA profiles that may have a match, the RapidLINK Match Application generates a printable report that summarizes the likelihood of the overall profile match and details the probability of each allelic match. For use only with Applied Biosystems RapidLink Software v1.0.

Features of the RapidLINK Match Application include:
• Powerful search tool
• Flexible with regard to size of pool
• Reduced workload for lab personnel
• Passive search is always “on”
• User-defined search parameters
• Automatically generated match report