October 2018 New Products

HID Ion GeneStudio™ S5 Plus System (Ion Torrent™)

The HID Ion GeneStudio S5 Plus System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows for forensic analysis. From targeted SNP panels to Precision ID STR workflows and mitochonidrial DNA sequencing, the HID Ion GeneStudio S5 Plus System is designed to enable flexibility to help labs manage small and large projects across multiple applications. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of high-throughput sequencing for forensic applications.

Features of the HID Ion GeneStudio S5 Plus System include:
• Select the chip (Ion 510–550 chip) that fits your application and throughput needs to achieve from 2–130 million reads per run
• Reduce setup time and complexity with cartridge-based plug-and-play reagents
• Complete run and analysis in hours and move forward with your experiments

The Ion GeneStudio S5 Series
The HID Ion GeneStudio S5 Series sequencers share the same foundational technology and fluidic architecture across all three instruments: the HID Ion GeneStudio S5 System, this HID Ion GeneStudio S5 Plus System, and the HID Ion GeneStudio S5 Prime System. The primary difference across the three instruments is on-sequencer analysis time.

Speed
The HID Ion GeneStudio S5 Series sequencers leverage the speed of semiconductor sequencing to enable the production of high-quality sequencing data in a few hours and enable you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time when paired with the HID Ion Chef System.

Simplicity
With single-day installation, a straightforward user interface, and cartridge-based reagents, the HID Ion GeneStudio S5 Series sequencers are easy to use. Combined with Precision ID library prep technology for target selection, the HID Ion Chef System for automated library and template preparation, and Converge Software for automated analysis, forensic sequencing is simpler than ever.

Small sample input
Low input DNA amounts from challenging sample types such as touch DNA or other forensic samples can be difficult to sequence on competitor next-generation sequencers. However, with Precision ID library preparation and sequencing technology and the HID Ion GeneStudio S5 Series sequencers, you can use as little as 100 pg of DNA input and achieve accurate, robust results.

Scalability
Why buy multiple systems for your lab when you can modulate your throughput by chip selection on a single next-generation sequencer? The Ion 5 Series chips that run on the HID Ion GeneStudio S5 Series systems provide several throughput options, so your lab has the flexibility to scale from small to large projects, enabling multiple forensic applications on a single system.

Simple analysis and storage solutions
Torrent Suite Software and Converge Software make NGS easy for beginners and experts alike. Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server). Integrate, annotate, and interpret variants using Converge Software (Thermo Fisher Cloud or local options available based on your needs).

Services and support
With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.

Service and support plans—comprehensive plans to maximize instrument performance and help ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

SureQuant™ AKT Isoform Panel (Relative Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Isoform Panel (Relative Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of AKT1, AKT2, and AKT3 isoforms using mass spectrometry (MS).

The SureQuant AKT Isoform Panel (Relative Quantitation) consists of two modules:
SureQuant AKT Isoform IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich AKT1, AKT2, and AKT3 proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant AKT Isoform Relative Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide mixture for relative peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant AKT Isoform Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate isoforms and phosphorylation of AKT
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This kit is ideal for monitoring AKT expression levels of the three isoforms and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Isoform IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant AKT Isoform Relative Quantitation Module provides reagents for relative quantitation of six unique peptides from AKT1, AKT2, and AKT3 isoforms using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

HID Ion GeneStudio™ S5 Prime System (Ion Torrent™)

The HID Ion GeneStudio S5 Prime System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows. For core and service labs requiring maximum throughput and rapid turnaround time from a benchtop NGS system, the HID Ion GeneStudio S5 Prime System enables unparalleled breadth and depth for large panels with the fastest turnaround time needs. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of forensic applications including mitochondria sequencing, STR analysis, and SNP genotyping.

Features of the HID Ion GeneStudio S5 Prime System include:
• Select the chip (Ion 510–550 chip) that fits your application and throughput needs to achieve from 2–130 million reads per run
• Reduce setup time and complexity with cartridge-based plug-and-play reagents
• Complete run and analysis in hours and move forward with your experiments

The Ion GeneStudio S5 Series
The HID Ion GeneStudio S5 Series sequencers share the same foundational technology and fluidic architecture across all three instruments: the HID Ion GeneStudio S5 System, the HID Ion GeneStudio S5 Plus System, and this HID Ion GeneStudio S5 Prime System. The primary difference across the three instruments is on-sequencer analysis time.

Speed
The HID Ion GeneStudio S5 Series sequencers leverage the speed of semiconductor sequencing to enable the production of high-quality sequencing data in a few hours and enable you to go from DNA library to data in as little as 24 hours with only 45 minutes of total hands-on time when paired with the HID Ion Chef System.

Simplicity
With single-day installation, a straightforward user interface, and cartridge-based reagents, the HID Ion GeneStudio S5 Series sequencers are easy to use. Combined with Precision ID library prep technology for target selection, the HID Ion Chef System for automated library and template preparation, and Converge Software for automated analysis, forensic sequencing is simpler than ever.

Small sample input
Low input DNA amounts from challenging sample types such as touch DNA or other forensic samples can be difficult to sequence on competitor next-generation sequencers. However, with Precision ID library preparation and sequencing technology and the HID Ion GeneStudio S5 Series sequencers, you can use as little as 100 pg of DNA input and achieve accurate, robust results.

Scalability
Why buy multiple systems for your lab when you can modulate your throughput by chip selection on a single next-generation sequencer? The Ion 5 Series chips that run on the HID Ion GeneStudio S5 Series systems provide several throughput options, so your lab has the flexibility to scale from small to large projects, enabling multiple forensic applications on a single system.

Simple analysis and storage solutions
Torrent Suite Software and Converge Software make NGS easy for beginners and experts alike. Plan, monitor, track, and analyze your runs using Torrent Suite Software (pre-installed on the Torrent Server). Integrate, annotate, and interpret variants using Converge Software (Thermo Fisher Cloud or local options available based on your needs).

Services and support
With more than 2,000 field personnel, Thermo Fisher Scientific has the most extensive network of dedicated Field Application Specialists and Service Engineers to work with you, so you can be confident that we will be there when and where you need us most.

Service and support plans—comprehensive plans to maximize instrument performance and help ensure availability of critical systems
Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications
Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation
Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications
DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data

SureQuant™ AKT Isoform IP and MS Sample Preparation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Isoform IP and MS Sample Preparation Module provides a complete reagent solution for the multiplexed enrichment and sample preparation of AKT1, AKT2, and AKT3 for mass spectrometry (MS).

SureQuant AKT Isoform IP and MS Sample Preparation Module features include:
Complete—includes all of the reagents necessary for sample lysis, immuno-enrichment, and MS sample preparation
Validated—antibodies validated for specificity and background
Optimized—procedure simplified and condensed to prepare samples after immunoprecipitation in four hours
Multiplex—able to immuno-enrich AKT1, AKT2, and AKT3 isoforms in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This module is ideal for immuno-enrichment of the AKT1, AKT2, and AKT3 isoforms for use with the SureQuant quantitation modules.

The SureQuant AKT Isoform IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

This kit can be used with the either the SureQuant AKT Isoform Absolute Quantitation Module or the SureQuant AKT Isoform Relative Quantitation Module for protein targeted quantitation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ AKT Isoform Relative Quantitation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Isoform Relative Quantitation Module provides a complete quantitation solution for the analysis of key AKT expression using mass spectrometry (MS).

SureQuant AKT Isoform Relative Quantitation Module features include:
Complete—contains validated AQUA HeavyPeptide mix, diluent, matrix, system suitability standard including optimized acquisition and analysis methods
Validated—peptides rigorously tested for successful quantitation of each target peptide
Multiplex—able to quantitate AKT1, AKT2, and AKT3 isoforms and phosphorylation of AKT

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This kit is ideal for monitoring AKT expression levels of the three isoforms and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Isoform Relative Quantitation Module provides reagents for relative quantitation of six unique peptides from AKT using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

This kit can be used with SureQuant AKT Isoform IP and MS Sample Preparation Module for enriched multiplex sample preparation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

Angiogenesis 3-Plex Human ProcartaPlex™ Panel 2 (Invitrogen™)

The Human Angiogenesis II 3-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 3 molecular mediators involved in blood vessel growth using Luminex xMAP technolog. Angiogenesis, the formation of new blood vessels, plays a major role in cancer progression, metastatic spread, as well as many other diseases. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay
Target list: angiogenin; angiostatin; endostatin
Bead regions: angiogenin: 12; angiostatin: 73; endostatin: 15

SureQuant™ AKT Isoform Absolute Quantitation Module (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Isoform Absolute Quantitation Module provides a complete quantitation solution for the analysis of key AKT expression using mass spectrometry (MS).

SureQuant AKT Isoform Absolute Quantitation Module features include:
Complete—contains validated AQUA HeavyPeptide and LightPeptide mixtures, diluent, matrix, system suitability standard including optimized acquisition and analysis methods
Validated—peptides rigorously tested for successful quantitation of each target peptide
Multiplex—able to quantitate AKT1, AKT2, and AKT3 isoforms and phosphorylation of AKT

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and AKT plays a vital role in the signaling cascade. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This kit is ideal for monitoring AKT expression levels of the three isoforms and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Isoform Absolute Quantitation Module provides reagents for absolute quantitation of six unique peptides from AKT using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

This kit can be used with SureQuant AKT Isoform IP and MS Sample Preparation Module for enriched multiplex sample preparation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ TP53 Panel (Absolute Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant TP53 Panel (Absolute Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of TP53 expression and phosphorylation using mass spectrometry (MS).

The SureQuant TP53 Panel (Absolute Quantitation) consists of two modules:
SureQuant TP53 Panel IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich TP53 and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant TP53 Panel Absolute Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide and LightPeptide mixtures for absolute peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant TP53 Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate expression and phosphorylation of TP53
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The tumor suppressor protein TP53 functions as a negative checkpoint regulator for abnormal cell signaling and can induce cell cycle arrest, activate DNA repair proteins, or initiate apoptosis. Mutations in TP53 often result abnormal cell growth and are commonly found in many types of cancer. This kit is ideal for monitoring TP53 expression and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant TP53 Panel IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant TP53 Panel Absolute Quantitation Module provides reagents for absolute quantitation of four unique peptides from TP53 using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

PRKCB2 (PKC beta II) Recombinant Human Protein (Invitrogen™)

The PKC family of serine/threonine kinases, including PRKCB2 (PKC beta II), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

IL-1 alpha Human ProQuantum Immunoassay Kit (Invitrogen™)

IL-1 alpha Human ProQuantum Immunoassay Kit

SureQuant™ AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of key phosphorylation events of AKT signaling pathway proteins using mass spectrometry (MS).

The SureQuant AKT Pathway (Phospho) Multiplex Panel (Relative Quantitation) consists of two modules:
SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module includes reagents necessary to immuno-enrich phosphorylated AKT-mTOR pathway proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant AKT Pathway (Phospho) Relative Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide mix for relative peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant AKT Pathway (Phospho) Multiplex Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate phosphorylation of ten key proteins in the AKT signaling pathway
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key phosphorylated proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant AKT Pathway (Phospho) IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, with non-specific proteins removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin, and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant AKT Pathway (Phospho) Relative Quantitation Module provides reagents for relative quantitation of 30 unique peptides from 10 phosphorylated AKT-mTOR signaling pathway target proteins using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the phosphorylated target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ RAS Isoform Panel (Absolute Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant RAS Isoform Panel (Absolute Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of NRAS, HRAS, and KRAS isoforms using mass spectrometry (MS).

The SureQuant RAS Isoform Panel (Absolute Quantitation) consists of two modules:
SureQuant RAS Isoform IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich NRAS, KRAS, and HRAS proteins and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant RAS Isoform Absolute Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide and LightPeptide mixtures for absolute peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instrumentation and Nanospray LC instrumentation

SureQuant RAS Isoform Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate isoforms of RAS
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of the RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This kit is ideal for monitoring expression levels of the three isoforms in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant RAS Isoform IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant RAS Isoform Absolute Quantitation Module provides reagents for absolute quantitation of three unique peptides from NRAS, KRAS, and HRAS isoforms using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mix, AQUA Ultimate LightPeptide mix, and Pierce 6 Protein Digest, equimolar, LC-MS grade reagents are used to generate a standard curve in a matrix, and serial dilutions performed by the user have been optimized to span 2–3 orders of magnitude (200 to 0.03 fmol on column). Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

SureQuant™ TP53 Panel (Relative Quantitation) (Thermo Scientific™)

The Thermo Scientific SureQuant TP53 Panel (Relative Quantitation) provides a complete reagent and quantitation solution for the enrichment and analysis of TP53 expression and phosphorylation using mass spectrometry (MS).

The SureQuant TP53 Panel (Relative Quantitation) consists of two modules:
SureQuant TP53 Panel IP and MS Sample Preparation Module includes all of the reagents necessary to immuno-enrich TP53 and perform in-solution MS sample preparation from sample lysis through peptide clean-up
SureQuant TP53 Panel Relative Quantitation Module includes a system suitability standard to monitor MS performance, AQUA Ultimate HeavyPeptide mix for relative peptide quantitation, and methods for using Thermo Scientific Q Exactive and Fusion Tribrid instruments and Nanospray LC instrumentation

SureQuant TP53 Panel features include:
Complete—includes all reagents for successful monitoring of MS system performance, sample preparation, and absolute quantitation of target proteins/peptides
Validated—antibodies, peptides, and control lysate rigorously tested for specificity and successful quantitation of each target peptide
Multiplex—able to quantitate expression and phosphorylation of TP53
Flexible—modular format allows for immuno-enrichment only, or in combination with relative or absolute quantitation

The tumor suppressor protein TP53 functions as a negative checkpoint regulator for abnormal cell signaling and can induce cell cycle arrest, activate DNA repair proteins, or initiate apoptosis. Mutations in TP53 often result abnormal cell growth and are commonly found in many types of cancer. This kit is ideal for monitoring TP53 expression and phosphorylation status in a single assay. The ability to simultaneously monitor and quantitate in a single sample is a powerful tool for assessment of normal and abnormal pathway signaling.

The SureQuant TP53 Panel IP and MS Sample Preparation Module enables highly effective antigen immunoprecipitation (IP) and co-immunoprecipitation (co-IP) for MS analysis. Cells are first lysed and incubated with an IP-MS-verified biotinylated antibody mixture. The antibody-antigen complex is then captured on MS-compatible streptavidin magnetic beads, where non-specific proteins are removed through washing with MS-compatible buffers. The target proteins are eluted using trypsin and are directly compatible with MS sample preparation. The MS sample preparation has been streamlined and can be completed in ~4 hours for same-day MS analysis.

The SureQuant TP53 Panel Relative Quantitation Module provides reagents for relative quantitation of four unique peptides from TP53 using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

Together, these modules provide a complete solution for sample preparation and analysis of the target proteins.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

PRKCD (PKC delta) Recombinant Human Protein (Invitrogen™)

The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.

Applied Biosystems™ RapidHIT™ ID System (Applied Biosystems™)

The Applied Biosystems RapidHIT ID System is a fast and simple-to-use instrument that produces trusted lab-quality forensic DNA profiles in 90 minutes. The system integrates sample preparation and capillary electrophoresis to generate DNA profiles that are aggregated within Applied Biosystems RapidLINK Software for direct upload to the database of choice or for further review and analysis.

The RapidHIT ID System delivers the reliable, reproducible, high-quality data you have come to expect from the Applied Biosystems brand. This system is for use with Applied Biosystems RapidLINK Software v1.0 and its v1.0 applications.

Features of the Applied Biosystems RapidHIT ID System include:
• Fast 90-minute system run time
• 1-minute hands-on time with integrated sample cartridge
• Consumables tracking through radio frequency identification (RFID)
• Intuitive touchscreen interface
• Facial recognition and barcode camera
• Fingerprint reader
• Up to six months of on-instrument time for the primary cartridge with refrigeration mechanism
• Expert system-capable
• Sample/data accessioning workflow-capable