October 2019 New Products

Neuroscience 18-plex Human ProcartaPlex™ Panel (Invitrogen™)

The Human Neuroscience 18-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 18 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: serum, plasma, csf
Sample volume:25 µL
Reported application: multiplex immunoassay
Target list: Tau (Total); Tau [pT181]; Amyloid beta 1-42; Neurogranin (NRGN); Neurofilament heavy (NF-H); GFAP; UCHL1; S100B; CHI3L1/YKL-40; GDNF; CNTF; BDNF; NGF-beta; MIF; NCAM-1; Kallikrein-6 (KLK6); TDP-43; FGF-21
Bead regions: Tau (Total): 46; Tau [pT181]: 61; Amyloid beta 1-42: 42; Neurogranin (NRGN): 75; Neurofilament heavy (NF-H): 33; GFAP: 22; UCHL1: 19; S100B: 18; CHI3L1/YKL-40: 34; GDNF: 44; CNTF: 36; BDNF: 57; NGF-beta: 55; MIF: 67; NCAM-1: 48; Kallikrein-6 (KLK6): 14; TDP-43: 74; FGF-21: 76

EasyPep™ Lysis Buffer (Thermo Scientific™)

Thermo Scientific EasyPep Lysis Buffer has been optimized for use with the EasyPep MS Sample Preparation kits to yield high quality proteomic samples for MS analysis.

Features of EasyPep Lysis Buffer include:
Optimized—efficient lysis of cells, tissues and plasma for MS proteomic sample preparation
Compatible—designed for use with EasyPep MS Sample Prep kits and MS sample preparation
Easy to use—pre-formulated reagent for efficient protein extraction

EasyPep Lysis Buffer has been formulated to efficiently extract proteins from cells, tissue, and plasma for MS sample preparation. This lysis reagent has been designed specifically for use with the EasyPep MS Sample Preparation kits and is compatible with the Pierce BCA or Rapid Gold BCA protein assays.

CountBright™ Plus Absolute Counting Beads (Invitrogen™)

CountBright Plus Absolute Counting Beads are a calibrated suspension of ~4 µm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm. In instances where a flow cytometer is not designed to provide absolute cell count, the use of absolute counting beads can help to determine the count. Absolute cell count is defined as the concentration of cells in the original sample.

• Beads are on-scale with most cell types, including human whole lysed blood, for ease of use
• >95% singlets for improved accuracy
• Excited by UV-IR lasers for an expanded emission range (385–860 nm)

MicroSEQ™ ID Microbial Identification Software, Lite, version 3.1.3 (Applied Biosystems™)

MicroSEQ ID Microbial Identification Software, Lite, version 3.1.3, allows you to easily identify and classify bacterial or fungal sequences by comparing them to a validated microbial library (available separately). Theis Lite version of the software is for use on a stand-alone PC (not connected to the genetic analyzer) for data analyses only.

Features of MicroSEQ ID Microbial Identification Software include:
• Automatically identify unknown specimens against a validated microbial library of over 3000 microbial sequences
• Run plates directly on Applied Biosystems 3500 Series genetic analyzers
• Equipped with security, audit, and e-signature features to enable 21 CFR Pt. 11 compliance

Confidently identify microbes
The MicroSEQ ID sequence libraries (available separately) consist of rigorously validated bacterial and fungal libraries to ensure accurate identification. The MicroSEQ ID 16S rDNA 500 Microbial Library consists of over 2300 500-bp 16S rDNA sequences, including extensive coverage of gram-negative non-fermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. The entries contain the first 500 bases of the 16S rRNA gene, sufficient to identify bacterial unknowns.

The MicroSEQ ID Fungal Gene Library contains 1300 reference sequences from the D2 region of the Large Subunit (rDNA) of yeast and mold species against which an unknown yeast or mold sequence can be compared in order to identify the organism. The MicroSEQ libraries contain information on correctly identified organisms and quality-controlled sequences derived from type strains of curated culture collections. The libraries also support sequence polymorphisms and include bacterial Category A biothreat agents.

Automatically identify unknown specimens
Reviewing each specimen in a project file to make a manual identification call can be tedious and time consuming. The Auto-ID feature in MicroSEQ ID software uses a set of editable parameters to automatically identify unknown specimens. The Results table is displayed in the report with an editable Comments field for additional information or manual identification entries.

Run plates directly
Applied Biosystems 3500 Series genetic analyzer users are able to run plates directly via the MicroSEQ ID software user interface. A single software wizard enables you to set up projects, specimens, and plates; adjust the plate layout via drag and drop; start and monitor the run; and review results.

The MicroSEQ system: a complete, integrated solution
Streamlining every step in microbial identification, the MicroSEQ system combines the advantages of MicroSEQ ID software with easy-to-use PrepMan Ultra sample preparation reagents and protocols, MicroSEQ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To help ensure optimal performance and system-wide integration, we develop and test all components of the system together. MicroSEQ ID software also includes security, audit, and electronic signature features, enabling the software to smoothly integrate into your workflow.

Immuno-Oncology Checkpoint 10-Plex Human ProcartaPlex™ Panel 3 (Invitrogen™)

The Human Immuno-Oncology Checkpoint 10-Plex ProcartaPlex Panel 3 is a preconfigured multiplex immunoassay kit that measures 10 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume:CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay
Target list: ICOS Ligand (B7-H2); CD276 (B7-H3); VISTA (B7-H5); B7-H6; CD47 (IAP); CD48 (BLAST-1); Galectin-9; TIMD-4; CD134 (OX40); S100A8/A9
Bead regions: ICOS Ligand (B7-H2): 34; CD276 (B7-H3): 72; VISTA (B7-H5): 64; B7-H6: 42; CD47 (IAP): 74; CD48 (BLAST-1): 19; Galectin-9: 38; TIMD-4: 39; CD134 (OX40): 55; S100A8/A9: 76

CD134 (OX40) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CD134 (OX40) Simplex ProcartaPlex kit measures CD134 (OX40) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay

SuperSignal™ West Atto Ultimate Sensitivity Substrate (Thermo Scientific™)

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignal West Atto substrate provides the highest-level sensitivity and better signal-to–noise ratios compared to other commercially available high-performance horseradish peroxidase (HRP) substrates.

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate characteristics include:
• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is designed for superior sensitivity and signal-to–noise ratios for very low abundant targets or precious samples in western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Atto substrate enables detection of target proteins in amounts that are too small to be seen with typical high performance ECL substrates.

Alexa Fluor™ Plus 555 Phalloidin (Invitrogen™)

Alexa Fluor Plus 555 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, orange/TRITC fluorescent Alexa Fluor Plus 555 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 555 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 555 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 3–4 times the sensitivity of Alexa Fluor 555 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 555/565 nm. Compatible with standard TRITC/RFP filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides "death cap" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

B7-H6 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human B7-H6 Simplex ProcartaPlex kit measures B7-H6 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay

SlowFade™ Glass Soft-set Antifade Mountant (Invitrogen™)

SlowFade Glass Antifade Mountant is a glycerol-based, ready-to-use, non-curing mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated to have a refractive index of 1.52 for sharper images and antifade components to resist photobleaching. The result is sharp, bright images with almost any fluorophore, with wide-field, confocal, or super-resolution microscopes, at focal depths of 0–500 µm.

Since SlowFade Glass Antifade Mountant is non-curing, cells can be imaged immediately after mounting, without any of the adverse effects on cell morphology that a curing mountant might cause. It comes ready to use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

If a curing, hard-set mountant is needed, also with a refractive index of 1.52, for years of slide storage, then Prolong Glass Antifade Mountant is recommended. See Mounting Media and Antifades to learn more about our complete collection of mountants.

Features of SlowFade Glass Antifade Mountant include:
• Ready-to-use, glycerol-based, non-curing antifade mountant
• Refractive index of 1.52, with minimized spherical aberrations
• Up to three times better axial resolution compared to regular mountant
• Visible tissue optical clearing, even in sections up to 1-mm thick
• Sharp, bright images at any focal depth from 0 to 500 µm

Sharp images for deep 3D imaging
SlowFade Glass Antifade Mountant features a refractive index of 1.52 (similar to glass coverslips), with superior photobleach protection. It is compatible with immersion oil and oil-immersion microscope optics, enabling a refraction-free light path and minimized spherical aberration, which improves axial resolution by three times (at 150-µm focal depth) as measured by point spread function. When 1-mm thick tissue sections are mounted, there is visible optical tissue clearing, enabling deep 3D imaging. For 3D imaging of whole organs (up to 10 mm), please see Clearing Reagents for Imaging 3D Cell Culture and Tissue.

Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, Slowfade Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes, and fluorescent proteins, including GFP, RFP, and mCherry. SlowFade Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.

Non-curing mountant which does not expand or shrink specimens
Most tissue clearing techniques either expand or shrink the specimens, causing artifacts in specimen morphology. SlowFade Glass Antifade Mountant is non-curing and does not cause expansion or contraction of specimens, which we have verified using Hela spheroids and rat brain sections. This characteristic allows 3D imaging of truly native specimen morphology. The specimens are available for imaging immediately after mounting, and properly sealed slides can be stored for weeks or months for excellent imaging results.

S100A8/A9 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human S100A8/A9 Simplex ProcartaPlex kit measures S100A8/A9 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay

Luminex™ MAGPIX™ Instrument System (Invitrogen™)

The Luminex MAGPIX Instrument System is the simplest, most affordable, and most compact Luminex instrument. This image-based multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96-well-plate in just 60 minutes. The Luminex MAGPIX system is compatible with ProcartaPlex multiplex immunoassays and QuantiGene Plex multiplex gene expression assays.

Features of the Luminex MAGPIX system include:
Multiplexing: up to 50 analytes per sample
Dynamic range (typical): ≥ 3.5 logs
Read time: 96-well plate in ≤ 60 min (up to 4,800 tests/hour)
Daily start-up/shut-down: ≤ 15 min

Available options
Software: extra seat licenses, Basic Security Module, 21 CFR part 11 Module, Automation Module, LIS/LIMS Module
Service: service contracts, IQ/OQ

Learn more:
ProcartaPlex multiplex immunoassays
QuantiGene Plex multiplex gene expression assays

Luminex xMAP technology
The open architecture of Luminex xMAP technology uses imaging, microspheres, digital signal processing, and traditional chemistry—combining proven technologies in a unique way.

Advantages of Luminex xMAP technology include:
• Helps reduce costs and labor through multiplexing
• Smaller sample size requirements compared to single result assays
• Enables fast, reproducible results from favorable kinetics of liquid bead array approach
• Broad coverage of applications, including protein expression and gene expression profiling

How Luminex xMAP technology works
Sets of magnetic microspheres are color-coded with combinations of red and infrared fluorophores. Each bead set is coated with a reagent specific to a particular bioassay, allowing the capture and detection of several specific analytes from a single sample. Optics in the compact analyzer excite the internal dyes that identify each microsphere as well as any reporter dye captured, providing quantitation of individual analytes on each microsphere. The analyzer reads many beads from each set, enabling rapid and precise results for several targets within a single sample.

CD276 (B7-H3) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CD276 (B7-H3) Simplex ProcartaPlex kit measures CD276 (B7-H3) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay

CD47 (IAP) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CD47 (IAP) Simplex ProcartaPlex kit measures CD47 (IAP) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: CCS: 50 µL; serum, plasma: 25 µL
Reported application: multiplex immunoassay

Alexa Fluor™ Plus 647 Phalloidin (Invitrogen™)

Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep red fluorescent Alexa Fluor Plus 647 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 647 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 647 Phalloidin per length of F-actin.

View our complete selection of Alexa Fluor and Alexa Fluor Plus conjugated phalloidin for F-actin staining ›

• Selectively stains F-actin with 2–3 times the sensitivity of Alexa Fluor 647 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 650/668 nm. Compatible with standard Cy5/deep red/far red filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples

Enables superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides "death cap" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.