PCR-Based Drug Discovery Assays

TaqMan™ Array, Human Developmental Phases of Colorectal Cancer, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Developmental Phases of Colorectal Cancer 96-well Plate contains 28 assays to Developmental Phases of Colorectal Cancer associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate.

TaqMan™ OpenArray™ Human Kinome Panel, QuantStudio™ 12K Flex (Applied Biosystems™)

The TaqMan® OpenArray® Human Kinome Panel is an efficient, easy-to-use OpenArray® plate for the characterization of genes encoding human kinases. This panel targets 772 well-defined genes, plus 56 endogenous control genes. The format of the OpenArray® plate allows for 1–3 replicates to be run in parallel per plate.

The TaqMan® OpenArray® Human Kinome Panel is intended to capture the full set of protein kinases in the human genome. Genes were chosen based on the primary literature and public databases. In addition, there are 56 endogenous control genes against which the assays can be normalized. All assays in this panel have been bench-validated in our laboratories, so you can be sure that each assay is fully optimized.

The plate is pre-formatted and inventoried, cost-effective, convenient, and easy-to-use, without the need for expensive robotics. Results are reproducible and consistent across samples, studies, and labs—for the same data quality from plate-to-plate and lot-to-lot, even with different operators.

This panel is intended for use with the QuantStudio™ 12K Flex Real-time PCR system which can cycle and image 4 pates simultaneously, providing high-throughput results with ease.

TaqMan™ Array, Human DNA Damage Induced 14-3-3 Sigma, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human DNA Damage Induced 14-3-3 Sigma Signaling 96-well Plate contains 28 assays to DNA Damage Induced 14-3-3 Sigma Signaling associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate.

Protein Thermal Shift™ Dye Kit

Applied Biosystems® Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.

Save Time and Money with Your Sample Screening
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods. Protein Thermal Shift™ products that are run on our real-time PCR systems enable researchers to inexpensively and efficiently:

• Screen samples for relative protein thermal stability
• Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
• Screen samples for stability changes after protein point mutations or modifications
• Systematically identify suitable⁄optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
• Quickly QC protein preps to ensure protein integrity and⁄or purity

Two Kits to Choose From
The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 2000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.

Simplify Analysis with Protein Thermal Shift™ Software
Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Array Human Immune Response (Applied Biosystems™)

The Applied Biosystems® TaqMan® Array Human Immune Response 96-well Plate contains 92 assays to immune response associated genes and 4 assays to candidate endogenous control genes.

The panel of assays in this plate targets genes from immune system functions that fall into 9 classes: Cell Surface Receptors; Stress Response; Oxidoreductases; Proteases; Transcription Factors; Signal Transduction; Cytokines and Cytokine Receptors; Chemokines and Chemokine Receptors; and Cell Cycle and Protein Kinases. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format

TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction

TaqMan™ OpenArray™ Human Inflammation Panel, QuantStudio™ 12K Flex (Applied Biosystems™)

The TaqMan® OpenArray® Human Inflammation Panel is an efficient, easy-to-use OpenArray® plate for quantitative gene expression analysis of inflammation genes important in drug discovery.

The panel covers 586 genes that have been studied as targets for a range of inflammatory diseases, plus 21 endogenous control genes. The plate is pre-formatted and inventoried, cost-effective, convenient, and easy-to-use, without the need for expensive robotics. Results are reproducible and consistent across samples, studies, and labs—for the same data quality from plate-to-plate and lot-to-lot, even with different operators.

This panel is intended for use with the QuantStudio™ 12K Flex Real-time PCR system which can cycle and image 4 pates simultaneously, providing high-throughput results with ease.

TaqMan™ Array Human AHR Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human AHR Pathway 96-well Plate contains 28 assays to genes associated with the AHR pathway and 4 assays to candidate endogenous control genes. All assays are plated in triplicate.

TaqMan™ Array, Human DNA Methylation & Transcriptional Repression, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human DNA Methylation And Transcriptional Repression 96-well Plate contains 28 assays to DNA Methylation And Transcriptional Repression associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate.

TaqMan™ Array Human Inflammation (Applied Biosystems™)

The panel of assays in this plate targets genes involved in a broad range of inflammatory diseases. The genes fall into 4 classes:

• Channels (L-type calcium and Ligand gated);
• Enzymes and Inhibitors (Lipases, Kinases, Nitric Oxide Synthase, Phosphodiesterase, Prostaglandin Metabolism, and Proteases)
• Factors (Tumor necrosis factors, Nuclear factor Kappa-Β, Interleukin, Annexins, Kinnogens)
• Receptors (GPCR, IL Receptor family, Adhesion molecules, TNF receptors and Nuclear receptors)

Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate Applied Biosystems® TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format

TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction

TaqMan™ Array, Human PDGF Pathway, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human PDGF Pathway 96-well Plate contains 92 assays to PDGF pathway associated genes and 4 assays to candidate endogenous control genes.

TaqMan™ Array Human Apoptosis via Death Receptors (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Apoptosis Through Death Receptors 96-well Plate contains 44 assays to genes associated with death receptor mediated apoptosis and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.

TaqMan™ Array Human IL3 Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human IL-3 Pathway 96-well Plate contains 44 assays to genes associated with IL-3 pathways and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.

TaqMan™ Array Human JAK-STAT Pathway (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human JAK-STAT Pathway 96-well Plate contains 92 assays to genes associated with JAK-STAT pathways and 4 assays to candidate endogenous control genes.

TaqMan™ Array, Human DNA Repair Mechanism, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human DNA Repair Mechanisms 96-well Plate contains 92 assays to genes associated with DNA repair mechanisms and 4 assays to candidate endogenous control genes.

TaqMan™ Array, Human Hematopoiesis, Fast 96-well (Applied Biosystems™)

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Hematopoiesis 96-well Plate contains 92 assays to hematopoiesis associated genes and 4 assays to candidate endogenous control genes.