September 2018 New Products

ProFlex™ PCR System + Plastics Package, 3x32-well, 8-tube strips (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp 8-Tube Strips with Attached Domed Caps.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-µL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

Features include:
• Strip of eight tubes with attached domed caps that open and close independently to prevent contamination and evaporation
• Angled caps that provide efficient tube strip positioning in racks and thermal cycler blocks
• Etched A-H letter labeling on individual tubes that is visible when caps are open or closed
• Dual end tabs for proper labeling and easy handling
• Graduated 20-μL measuring markers on each tube for visual volume verification

CaptureSelect™ KappaXP Affinity Matrix (Thermo Scientific™)

CaptureSelect KappaXP Affinity Matrix is an improved version of CaptureSelect KappaXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is the same: the ligand binds the constant domain of the human kappa light chain, but it features an increased binding capacity (up to 45 g IgG/L), and a wider elution range (full elution up to pH 6.0) compared to other affinity resins that bind the kappa light chain.

Features of this affinity matrix include:
• Single-step purification of Ig’s and Fab and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Human specific, with no binding to bovine antibodies
• Excellent scalability
• Non-animal-derived

The high selectivity and yield obtained using CaptureSelect KappaXP Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

View other research-scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created through a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Fab binding capacity: 20–30 g Fab/L resin depending on flow rate, column height, and contact time
IgG binding capacity: 35–45 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris; 2.0 M MgCl2, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol

ProFlex™ PCR System + Plastics Package, 3x32-well, TriFlex plates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp TriFlex PCR Reaction Plates and MicroAmp 32-Well Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design, with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. The plates offer the same thin-wall construction as all other MicroAmp plastics, for excellent heat transfer from thermal block to sample, and they are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Designed for use with the ProFlex 3 x 32-well PCR System and all Applied Biosystems standard 96-well thermal cyclers
• Easy-to-tear dual side-tabs for simple separation into three individual 32-well plates
• Semi-skirted for easy handling and labeling
• Standard 250 µL working volume, with a 300 µL maximum well volume when used with an adhesive film
• Alphanumeric labeling on each 32-well plate for easy well identification
• Certified human DNA-, DNase-, and RNase-free

MicroAmp 32-Well Clear Adhesive Films
MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.

Key features:
• Same product design as the trusted MicroAmp Clear Adhesive Film for 96-well plates, but pre-cut to fit 32-well plates
• Easy to apply and remove—simply peel off the backing and apply firmly to the 32-well plate

ProFlex ™ PCR System + Plastics Package, 1x96-well, EnduraPlates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

SimpliAmp™ Thermal Cycler + Plastics Package, EnduraPlates (Applied Biosystems™)

This SimpliAmp Thermal Cycler package includes a thermal cycler plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

SimpliAmp Thermal Cycler
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch-screen and VeriFlex temperature control technology enable simple, accurate optimization. And now you can access your SimpliAmp cycler with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

For detailed instrument information and specifications, see the SimpliAmp Thermal Cycler.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

RAGE Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human RAGE Simplex ProcartaPlex kit measures RAGE protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

IL-12p70 Human ProQuantum Immunoassay Kit (Invitrogen™)

IL-12p70 Human ProQuantum Immunoassay Kit

PEPotec™ Immuno Custom Peptide Libraries (Thermo Scientific™)

Thermo Scientific PEPotec Immuno Custom Peptide Libraries are fully synthetic custom libraries that support high-throughput screening assays to map epitopes or identify immunogenic sites in proteins. They are fully customizable and supplied with acetate as the counter-ion to avoid potential toxicity issues in epitope discovery and mapping. These application-specific peptide libraries are ready to use in applications such as vaccine development, T- and-B cell research, antibody development, and biomarker discovery. Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events.

Learn more about our PEPotec Immuno Custom Peptide Libraries ›

Highlights:
Low toxicity—peptides with acetate as the counter-ion are less toxic in certain applications when compared with those with trifluoroacetate (TFA)
Application-specific—the 6- to 20–amino acid peptide length distribution fits most immunological applications
Convenient—peptides are provided in individual 2-D barcoded tubes in 96-tube plates, and each order includes a CD with the peptide sequences and positions in each plate
Flexible—optional services, including phosphorylation and acetylation, are available for studying signaling and regulatory proteins

Applications:
• Epitope mapping of B and T cells
• Vaccine development
• High-throughput peptide screening
• Biomarker discovery
• Cell signaling (kinase/protease studies)

For a quote or more information, email us at: peps@thermofisher.com.
For Europe, Middle East, and Africa, please email us at: europeservicespeptides@thermofisher.com.

Poly-D-Lysine (Gibco™)

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes

IL-7 Human ProQuantum Immunoassay Kit (Invitrogen™)

IL-7 Human ProQuantum Immunoassay Kit

POROS™ GoPure™ AAVX Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAVX 50 µm resin in this GoPure column has a novel AAVX CaptureSelect ligand, making it suited primarily for affinity capture of a broad range of adeno-associated virus (AAV) serotypes.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ GoPure™ MabCapture A Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS MabCapture A 50µm resin in this GoPure column has a protein A ligand immobilized to POROS backbone suitable for capture affinity chromatography.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

GeneChip™ PrimeView™ Global Gene Expression Profile Assay (Applied Biosystems™)

The GeneChip PrimeView Global Gene Expression Profile Assay enables expression profiling with an emphasis on established, well-annotated content. More than 36,000 transcripts and variants are measured per sample. Sequences used in the design of the array were selected from RefSeq version 36, UniGene database 219, and full-length human mRNAs from GenBank™.

Features include:
• Cost-effective global confirmation of pluripotency marker expression
• Simple-to-use online analysis tool
• Published method with over 15,000 samples analyzed
• For use with GeneChip Scanner 3000

In addition, the PluriTest™ Assay now accepts PrimeView array data from GeneChip Scanner 3000.

About the PluriTest Assay
The PluriTest Assay is a bioinformatics assay that compares the transcriptional profile of a sample to an extensive reference set of >450 cell/tissue types, including 223 hESC lines, 41 iPSC lines, somatic cells, and tissues. The online analysis tool confirms pluripotency marker expression via two separate scores: Pluripotency and Novelty. The Pluripotency Score informs the user how strongly a model-based pluripotency signature is expressed in the samples analyzed. The Novelty Score indicates the general model fit for a given sample.

More information on the PluriTest Assay ›

PEPotec™ SRM Custom Peptide Libraries (Thermo Scientific™)

Thermo Scientific PEPotec SRM Peptide Libraries are fully synthetic custom libraries for the development of mid- to high-throughput absolute quantitation with selective-reaction monitoring (SRM) or multiple-reaction monitoring (MRM) assays. The PEPotec SRM Peptide Libraries offer great convenience and flexibility for the development of quantitative mass spectroscopy (MS) with many customizable options. The standard service supplies a suspension of at least 0.1 mg of each crude peptide housed in individual tubes in a 96-well plate format with either arginine (R) or lysine (K) as the C-terminal amino acid (other C-terminal amino acids are available). Three quality control grades are available, and optional services and peptide modifications are offered to give you the peptide libraries that fit your experimental needs.

Learn more about our PEPotec SRM Custom Peptide Libraries ›

Highlights:
Traceable—peptides are provided in individual 2-D barcoded tubes in 96-tube plates
Customized—libraries available in various grades with optional services available
Convenient—standard libraries are delivered solubilized in 0.1% trifluoroacetic acid (TFA) in 50% (v/v) acetonitrile/water
Flexible—extensive list of available modifications

Applications:
• Mid- to high-throughput development of SRM and MRM assays
• MS workflows with relative and absolute quantitation strategies

Includes:
• Fully synthetic, crude (as synthesized) peptides
• Multiple grades of QC analysis and optional services and modifications
• Provided in individual Thermo Scientific Matrix 96-tube plates

For a quote or more information, email us at: peps@thermofisher.com.
For Europe, Middle East, and Africa, please email us at: europeservicespeptides@thermofisher.com.

ProFlex™ PCR System + Plastics Package, 1x96-well, 8-tube strips (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp 8-Tube Strips with Attached Domed Caps.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-µL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

Features include:
• Strip of eight tubes with attached domed caps that open and close independently to prevent contamination and evaporation
• Angled caps that provide efficient tube strip positioning in racks and thermal cycler blocks
• Etched A-H letter labeling on individual tubes that is visible when caps are open or closed
• Dual end tabs for proper labeling and easy handling
• Graduated 20-μL measuring markers on each tube for visual volume verification