September 2018 New Products

HCT 116 (+hIGF-1) Control Lysate (1mg/mL) (Thermo Scientific™)

Thermo Scientific HCT 116 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the RAS signaling pathway.

HCT 116 (+hIGF-1) Control Lysate features include:
• Validated for the expression RAS by western blotting and of NRAS, KRAS, and HRAS by mass spectrometry analysis
• Optimized—stimulation and lysis conditions optimized for detection of RAS signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This lysate has been validated for expression levels of the three isoforms of RAS using mass spectrometry.

The HCT 116 cell line is a human colon cancer epithelial cell line and has been used for proliferation and tumorigenicity studies for colon cancer. The HCT 116 (+hIGF-1) cell lysate is provided as frozen lysate at a standard concentration of 1 mg/mL (500 mg per vial). Lysates are prepared in non-denaturing buffer without loading dyes or dissociating agents (i.e., no 2-mercaptoethanol or dithiothreitol has been added). For western blot analysis, heat lysate to 95°C for 5 minutes. Add reducing agent before heating if desired. The recommended loading volume per lane is 10–20 μL (10–20 µg).

Related products
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
SureQuant RAS Isoform Panel (Absolute Quantitation)
SureQuant TP53 Panel (Absolute Quantitation)

POROS™ GoPure™ XS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS XS resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

CD36 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CD36 Simplex ProcartaPlex kit measures CD36 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

MiniAmp™ Plus Thermal Cycler + Plastics Package, 8-tube strips (Applied Biosystems™)

This MiniAmp Plus Thermal Cycler package includes a thermal cycler plus MicroAmp 8-Tube Strips with Attached Domed Caps.

The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. Innovations like a responsive color touch-screen and VeriFlex Blocks temperature-control technology enable simple, accurate optimization. The MiniAmp Plus Thermal Cycler can be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Key features and benefits:
VeriFlex Blocks—with three independent temperature zones for precise temperature control over your PCR optimization
5-inch intuitive color touch-screen—for easy programming and training of new users
Small footprint—with dimensions of 19-cm wide and 39-cm deep, you can fit the MiniAmp Plus Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer

For detailed instrument information and specifications, see the MiniAmp Plus Thermal Cycler.

MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-µL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

Features include:
• Strip of eight tubes with attached domed caps that open and close independently to prevent contamination and evaporation
• Angled caps that provide efficient tube strip positioning in racks and thermal cycler blocks
• Etched A-H letter labeling on individual tubes that is visible when caps are open or closed
• Dual end tabs for proper labeling and easy handling
• Graduated 20-μL measuring markers on each tube for visual volume verification

ProFlex™ PCR System + Plastics Package, 2x384-well, EnduraPlates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

POROS™ GoPure™ AAVX Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAVX 50 µm resin in this GoPure column has a novel AAVX CaptureSelect ligand, making it suited primarily for affinity capture of a broad range of adeno-associated virus (AAV) serotypes.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

CRT (CALR) Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human CRT (CALR) Simplex ProcartaPlex kit measures CRT (CALR) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

A549 (+hIGF-1) Control Lysate (1mg/mL) (Thermo Scientific™)

Thermo Scientific A549 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway.

A549 (+hIGF-1) Control Lysate features include:
• Validated for the expression of AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 proteins by western blotting and mass spectrometry analysis
• Optimized—stimulation and lysis conditions optimized for detection of AKT signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This lysate has been validated for expression of ten key proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2).

The A549 cell line is a human lung carcinoma cell line and is used as a model for non-small lung carcinoma. The A549 (+hIGF-1) cell lysate is provided as frozen lysate at a standard concentration of 1 mg/mL (500 mg per vial). Lysates are prepared in non-denaturing buffer without loading dyes or dissociating agents (i.e., no 2-mercaptoethanol or dithiothreitol has been added). For western blot analysis, heat lysate to 95°C for 5 minutes. Add reducing agent before heating if desired. The recommended loading volume per lane is 10–20 μL (10–20 µg).

Related products
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
SureQuant RAS Isoform Panel (Absolute Quantitation)
SureQuant TP53 Panel (Absolute Quantitation)

POROS™ GoPure™ HS Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS HS 50 µm resin in this GoPure column has a sulphopropyl functional group for strong cation exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

TYRO3 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human TYRO-3 Simplex ProcartaPlex kit measures TYRO-3 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

MiniAmp™ Thermal Cycler + Plastics Package, TriFlex plates (Applied Biosystems™)

This MiniAmp Thermal Cycler package includes a thermal cycler plus MicroAmp TriFlex PCR Reaction Plates and MicroAmp 32-Well Clear Adhesive Films.

The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. The MiniAmp Thermal Cycler has a 5-inch color touchscreen, making it easy to control, and it can also be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Key features and benefits:
Small footprint—with dimensions of 19-cm wide and 39-cm deep, you can fit the MiniAmp Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
5-inch intuitive color touchscreen—for easy programming and training of new users
WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer

For detailed instrument information and specifications, see the MiniAmp Thermal Cycler.

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design, with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. The plates offer the same thin-wall construction as all other MicroAmp plastics, for excellent heat transfer from thermal block to sample, and they are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Designed for use with the ProFlex 3 x 32-well PCR System and all Applied Biosystems standard 96-well thermal cyclers
• Easy-to-tear dual side-tabs for simple separation into three individual 32-well plates
• Semi-skirted for easy handling and labeling
• Standard 250 µL working volume, with a 300 µL maximum well volume when used with an adhesive film
• Alphanumeric labeling on each 32-well plate for easy well identification
• Certified human DNA-, DNase-, and RNase-free

MicroAmp 32-Well Clear Adhesive Films
MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.

Key features:
• Same product design as the trusted MicroAmp Clear Adhesive Film for 96-well plates, but pre-cut to fit 32-well plates
• Easy to apply and remove—simply peel off the backing and apply firmly to the 32-well plate

POROS™ GoPure™ AAVX Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAVX 50 µm resin in this GoPure column has a novel AAVX CaptureSelect ligand, making it suited primarily for affinity capture of a broad range of adeno-associated virus (AAV) serotypes.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

uPAR Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human uPAR Simplex ProcartaPlex kit measures uPAR protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

VetMAX™ MastiType Multi Combo Kit (Applied Biosystems™)

This combo kit includes the VetMAX MastiType Multi Kit and the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module (also available separately).

VetMAX MastiType Multi Kit
The VetMAX MastiType Multi Kit enables rapid, accurate detection of 15 mastitis-causing pathogens and one penicillin-resistance gene in bovine milk. These 16 markers are detected using real-time PCR amplification of DNA unique to each pathogen in four separate reactions. The kit detects these mastitis-causing pathogens:
Staphylococcus aureus
Staphylococcus spp. (including all major coagulase-negative staphylococci)
Streptococcus agalactiae
Streptococcus dysgalactiae
Streptococcus uberis
Escherichia coli
Enterococcus spp. (including E. faecalis and E. faecium)
Klebsiella oxytoca (and/or K. pneumoniae)
Serratia marcescens
Corynebacterium bovis
Trueperella pyogenes and/or Peptoniphilus indolicus
• Staphylococcal β-lactamase gene (penicillin-resistance gene)
Mycoplasma bovis
Mycoplasma spp.
• Yeast
Prototheca spp.

Learn more about the VetMAX MastiType Multi Kit ›

For Veterinary Use Only. For In Vitro Use Only.

MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module
The MagMAX CORE sample extraction system is a universal magnetic bead-based separation system designed to meet current and future veterinary testing needs. It is compatible with both automated (using the KingFisher Flex Purification System) and manual purification methods. This modular technology is optimized for the purification of nucleic acids from a wide range of sample matrices commonly submitted for veterinary diagnostic testing.

The MagMAX CORE Mastitis & Panbacteria Module is offered here with the MagMAX CORE Nucleic Acid Purification Kit to aid in the processing of all bacteria, especially those important in the detection of mastitis in cattle and from challenging matrices like mastitic milk. We have included a specially optimized lysis solution, along with an adjusted extraction procedure to improve the recovery of nucleic acid from all bacteria, including gram-positive bacteria.

Learn more about the MagMAX CORE Nucleic Acid Purification Kit with Mastitis & Panbacteria Module ›

For Laboratory Use Including Veterinary and Environmental Uses.

POROS™ GoPure™ AAV9 Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS CaptureSelect AAV9 50 µm resin in this GoPure column has a novel AAV9 CaptureSelect ligand, making it suited primarily for affinity capture of adeno-associated virus (AAV9) serotypes.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.