September 2018 New Products

RAGE Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human RAGE Simplex ProcartaPlex kit measures RAGE protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

IL-17F Human ProQuantum Immunoassay Kit (Invitrogen™)

IL-17F Human ProQuantum Immunoassay Kit for ELISA

ProFlex™ PCR System + Plastics Package, 3x32-well, TriFlex plates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp TriFlex PCR Reaction Plates and MicroAmp 32-Well Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design, with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. The plates offer the same thin-wall construction as all other MicroAmp plastics, for excellent heat transfer from thermal block to sample, and they are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Designed for use with the ProFlex 3 x 32-well PCR System and all Applied Biosystems standard 96-well thermal cyclers
• Easy-to-tear dual side-tabs for simple separation into three individual 32-well plates
• Semi-skirted for easy handling and labeling
• Standard 250 µL working volume, with a 300 µL maximum well volume when used with an adhesive film
• Alphanumeric labeling on each 32-well plate for easy well identification
• Certified human DNA-, DNase-, and RNase-free

MicroAmp 32-Well Clear Adhesive Films
MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.

Key features:
• Same product design as the trusted MicroAmp Clear Adhesive Film for 96-well plates, but pre-cut to fit 32-well plates
• Easy to apply and remove—simply peel off the backing and apply firmly to the 32-well plate

IL-4 Human ProQuantum Immunoassay Kit (Invitrogen™)

IL-4 Human ProQuantum Immunoassay Kit for ELISA

Poly-D-Lysine (Gibco™)

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes

GAS-6 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human GAS-6 Simplex ProcartaPlex kit measures GAS-6 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

TaqMan® PGx Express Panel, 384-well plate (Applied Biosystems™)

This TaqMan PGx (Pharmacogenomics) Express Panel is offered in pre-spotted 384-well plates, an efficient, easy-to-use format for pharmacogenomics applications. The TaqMan PGx Express Panel contains 64 TaqMan DME and SNP genotyping assays per 384-well plate. The assays were developed to detect polymorphisms in genes encoding drug metabolism enzymes (DMEs) and associated transport proteins. This panel provides coverage of essential, commonly studied markers within CYP2D6, CYP2C9, CYP2C19, and other important DME and clinical research genes.

Each TaqMan Drug Metabolism and SNP genotyping assay in the panel contains two allele-specific probes and a primer pair to detect the specific SNP target. Both the probes and primers uniquely align within the genome, enabling the TaqMan genotyping technology to provide superior specificity. It is this specificity that allows these assays to detect targets residing in highly homologous gene families that may include pseudogenes. TaqMan Drug Metabolism genotyping assays were developed using a high level of bioinformatics and wet-lab stringency. All assays have passed performance tests involving 180 unique DNA samples from four different populations.

The plate is pre-formatted and inventoried, cost-effective, convenient, and easy-to-use, without the need for expensive robotics. Results are reproducible and consistent across samples, studies, and labs—for the same data quality from plate-to-plate and lot-to-lot, even with different operators.

For fast delivery, all assays in this collection have been manufactured and placed into inventory and are ready to ship at ambient temperature.

MiniAmp™ Thermal Cycler + Plastics Package, TriFlex plates (Applied Biosystems™)

This MiniAmp Thermal Cycler package includes a thermal cycler plus MicroAmp TriFlex PCR Reaction Plates and MicroAmp 32-Well Clear Adhesive Films.

The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. The MiniAmp Thermal Cycler has a 5-inch color touchscreen, making it easy to control, and it can also be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Key features and benefits:
Small footprint—with dimensions of 19-cm wide and 39-cm deep, you can fit the MiniAmp Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
5-inch intuitive color touchscreen—for easy programming and training of new users
WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer

For detailed instrument information and specifications, see the MiniAmp Thermal Cycler.

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design, with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. The plates offer the same thin-wall construction as all other MicroAmp plastics, for excellent heat transfer from thermal block to sample, and they are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Designed for use with the ProFlex 3 x 32-well PCR System and all Applied Biosystems standard 96-well thermal cyclers
• Easy-to-tear dual side-tabs for simple separation into three individual 32-well plates
• Semi-skirted for easy handling and labeling
• Standard 250 µL working volume, with a 300 µL maximum well volume when used with an adhesive film
• Alphanumeric labeling on each 32-well plate for easy well identification
• Certified human DNA-, DNase-, and RNase-free

MicroAmp 32-Well Clear Adhesive Films
MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.

Key features:
• Same product design as the trusted MicroAmp Clear Adhesive Film for 96-well plates, but pre-cut to fit 32-well plates
• Easy to apply and remove—simply peel off the backing and apply firmly to the 32-well plate

HCT 116 (+hIGF-1) Control Lysate (1mg/mL) (Thermo Scientific™)

Thermo Scientific HCT 116 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the RAS signaling pathway.

HCT 116 (+hIGF-1) Control Lysate features include:
• Validated for the expression RAS by western blotting and of NRAS, KRAS, and HRAS by mass spectrometry analysis
• Optimized—stimulation and lysis conditions optimized for detection of RAS signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C

The RAS family of GTPase proteins modulates two key signaling pathways involved in cell growth and proliferation: the MAPK and PI3K pathways. Aberrant activation of RAS proteins promotes constitutive activation of cell growth and proliferation that can result in cancer. Mutations in the NRAS, KRAS, and HRAS oncogenes are the most common mutations in human cancer. This lysate has been validated for expression levels of the three isoforms of RAS using mass spectrometry.

The HCT 116 cell line is a human colon cancer epithelial cell line and has been used for proliferation and tumorigenicity studies for colon cancer. The HCT 116 (+hIGF-1) cell lysate is provided as frozen lysate at a standard concentration of 1 mg/mL (500 mg per vial). Lysates are prepared in non-denaturing buffer without loading dyes or dissociating agents (i.e., no 2-mercaptoethanol or dithiothreitol has been added). For western blot analysis, heat lysate to 95°C for 5 minutes. Add reducing agent before heating if desired. The recommended loading volume per lane is 10–20 μL (10–20 µg).

Related products
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
SureQuant RAS Isoform Panel (Absolute Quantitation)
SureQuant TP53 Panel (Absolute Quantitation)

ProFlex ™ PCR System + Plastics Package, 1x96-well, EnduraPlates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

Automated Thermal Cycler (ATC) + Plastics Package, 96-well, half-skirt EnduraPlates (Applied Biosystems™)

This Automated Thermal Cycler (ATC) package includes a thermal cycler plus MicroAmp EnduraPlates (half-skirt) and MicroAmp Clear Adhesive Films.

Automated Thermal Cycler (ATC)
For customers seeking to automate their PCR workflow, the Automated Thermal Cycler (ATC) provides the reliability and performance you’ve come to expect from Applied Biosystems instruments. Now in a small, easy-to-integrate, and compatible format, our Automated Thermal Cycler enables hands-free PCR results.

Space-saving design with our smallest thermal cycler footprint
• Flexible modular design helps save space on a robotic deck
• The separate control panel module provides flexibility when integrating on a robotic deck
• Choice of 10-cm, 1-m, and 3-m cables allows flexibility to locate the control module under the thermal cycler, under the deck, or up to 3 meteres away from the unit

Automated lid and cycler features
• The automated lid is compatible with programming for easy, hands-free operation with a liquid handler or plate stacker
• The heated cover slides forward to cover the plate nest, to help ensure thermal contact and eliminate evaporation
• The ATC is compatible with full-skirt or half-skirt plates

For detailed instrument information and specifications, see the Automated Thermal Cycler.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

MCP-2 Human ProQuantum Immunoassay Kit (Invitrogen™)

MCP-2 Human ProQuantum Immunoassay Kit for ELISA

POROS™ GoPure™ MabCapture A Select Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS MabCapture A Select 50 µm resin in this GoPure column has a CaptureSelect protein A ligand immobilized to POROS backbone suitable for capture affinity chromatography.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

POROS™ GoPure™ PI Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS PI 50 resin in this GoPure column has a polyethyleneimine functional group for strong anion exchange chromatography.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

PEPotec™ SRM Custom Peptide Libraries (Thermo Scientific™)

Thermo Scientific PEPotec SRM Peptide Libraries are fully synthetic custom libraries for the development of mid- to high-throughput absolute quantitation with selective-reaction monitoring (SRM) or multiple-reaction monitoring (MRM) assays. The PEPotec SRM Peptide Libraries offer great convenience and flexibility for the development of quantitative mass spectroscopy (MS) with many customizable options. The standard service supplies a suspension of at least 0.1 mg of each crude peptide housed in individual tubes in a 96-well plate format with either arginine (R) or lysine (K) as the C-terminal amino acid (other C-terminal amino acids are available). Three quality control grades are available, and optional services and peptide modifications are offered to give you the peptide libraries that fit your experimental needs.

Learn more about our PEPotec SRM Custom Peptide Libraries ›

Highlights:
Traceable—peptides are provided in individual 2-D barcoded tubes in 96-tube plates
Customized—libraries available in various grades with optional services available
Convenient—standard libraries are delivered solubilized in 0.1% trifluoroacetic acid (TFA) in 50% (v/v) acetonitrile/water
Flexible—extensive list of available modifications

Applications:
• Mid- to high-throughput development of SRM and MRM assays
• MS workflows with relative and absolute quantitation strategies

Includes:
• Fully synthetic, crude (as synthesized) peptides
• Multiple grades of QC analysis and optional services and modifications
• Provided in individual Thermo Scientific Matrix 96-tube plates

For a quote or more information, email us at: peps@thermofisher.com.
For Europe, Middle East, and Africa, please email us at: europeservicespeptides@thermofisher.com.

uPAR Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human uPAR Simplex ProcartaPlex kit measures uPAR protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

TYRO3 Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human TYRO-3 Simplex ProcartaPlex kit measures TYRO-3 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

IFN gamma Mouse ProQuantum Immunoassay Kit (Invitrogen™)

IFN gamma Mouse ProQuantum Immunoassay Kit for ELISA

MCF7 (+hIGF-1) Control Lysate (1mg/mL) (Thermo Scientific™)

Thermo Scientific MCF7 (+hIGF-1) Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the AKT signaling pathway.

MCF7 (+hIGF-1) Control Lysate features include:
• Validated for the detection and phosphorylation of AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 proteins
• Optimized—stimulation and lysis conditions optimized for detection of AKT signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation, and phosphorylation plays a major role in its modulation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This lysate has been validated for expression of ten key proteins and phosphorylation in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2).

The MCF-7 cell line is a human epithelial breast cancer cell line with estrogen, progesterone, and glucocorticoid receptor expression and is used as an in vitro model for breast cancer. The MCF7 (+hIGF-1) cell lysate is provided as frozen lysate at a standard concentration of 1 mg/mL (500 mg per vial). Lysates are prepared in non-denaturing buffer without loading dyes or dissociating agents (i.e., no 2-mercaptoethanol or dithiothreitol has been added). For western blot analysis, heat lysate to 95°C for 5 minutes. Add reducing agent before heating if desired. The recommended loading volume per lane is 10–20 μL (10–20 µg).

Related products
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
SureQuant RAS Isoform Panel (Absolute Quantitation)
SureQuant TP53 Panel (Absolute Quantitation)

MiniAmp™ Plus Thermal Cycler + Plastics Package, 8-tube strips (Applied Biosystems™)

This MiniAmp Plus Thermal Cycler package includes a thermal cycler plus MicroAmp 8-Tube Strips with Attached Domed Caps.

The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs. Innovations like a responsive color touch-screen and VeriFlex Blocks temperature-control technology enable simple, accurate optimization. The MiniAmp Plus Thermal Cycler can be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Key features and benefits:
VeriFlex Blocks—with three independent temperature zones for precise temperature control over your PCR optimization
5-inch intuitive color touch-screen—for easy programming and training of new users
Small footprint—with dimensions of 19-cm wide and 39-cm deep, you can fit the MiniAmp Plus Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer

For detailed instrument information and specifications, see the MiniAmp Plus Thermal Cycler.

MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-µL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

Features include:
• Strip of eight tubes with attached domed caps that open and close independently to prevent contamination and evaporation
• Angled caps that provide efficient tube strip positioning in racks and thermal cycler blocks
• Etched A-H letter labeling on individual tubes that is visible when caps are open or closed
• Dual end tabs for proper labeling and easy handling
• Graduated 20-μL measuring markers on each tube for visual volume verification

Flt-3 Ligand Human ProQuantum Immunoassay Kit (Invitrogen™)

Flt-3 Ligand Human ProQuantum Immunoassay Kit

CaptureSelect™ KappaXP Affinity Matrix (Thermo Scientific™)

CaptureSelect KappaXP Affinity Matrix is an improved version of CaptureSelect KappaXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is the same: the ligand binds the constant domain of the human kappa light chain, but it features an increased binding capacity (up to 45 g IgG/L), and a wider elution range (full elution up to pH 6.0) compared to other affinity resins that bind the kappa light chain.

Features of this affinity matrix include:
• Single-step purification of Ig’s and Fab and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Human specific, with no binding to bovine antibodies
• Excellent scalability
• Non-animal-derived

The high selectivity and yield obtained using CaptureSelect KappaXP Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

View other research-scale antibody affinity resins ›

Free of animal components
Our CaptureSelect products are affinity ligands created through a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Fab binding capacity: 20–30 g Fab/L resin depending on flow rate, column height, and contact time
IgG binding capacity: 35–45 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris; 2.0 M MgCl2, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol

MMP 3-Plex Human ProcartaPlex™ Panel 2 (Invitrogen™)

ProcartaPlex immunoassays are an ideal solution for assessing multiple protein biomarkers in a single sample. Luminex xMAP technology is used for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins. ProcartaPlex assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. We also offer a broad portfolio of pre-configurated panels and combinable single analytes.

It is recommended to dilute MMP-2, MMP-3, and MMP-9 samples when measured in serum, heparin plasma,or citrate plasma (EDTA plasma is not recommended). The recommended sample dilution factor is 1:50.

This kit does not contain a Multiscreen Filter Plate.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, heparin plasma, citrate plasma (EDTA plasma not recommended)
Sample volume: serum, heparin plasma, citrate plasma: 25 µL (1:50 pre-diluted); CCS: 50 µL
Reported application: multiplex immunoassay
Target list: MMP-2, MMP-3, MMP-9
Bead regions: MMP-2: 18; MMP-3: 19; MMP-9: 20
Assay range (as determined for Lot 1): MMP-2: 7.8–31900 pg/mL; MMP-3: 21.1–21650 pg/mL; MMP-9: 0.9–3750 pg/mL
Assay sensitivity (as determined for Lot 1): MMP-2: 16.13 pg/mL; MMP-3: 4.6 pg/mL; MMP-9: 0.2 pg/mL

BT-549 Control Lysate (1mg/mL) (Thermo Scientific™)

Thermo Scientific BT-549 Control Lysate is a stimulated cell lysate that has been validated for the expression levels and phosphorylation of key targets in the TP53 signaling pathway.

BT-549 Control Lysate features include:
• Validated for the expression of TP53 and phosphorylated TP53 by western blotting and mass spectrometry analysis
• Optimized—stimulation and lysis conditions optimized for detection of TP53 signaling targets
• Convenient—lysate is ready-to-use for immunoprecipitation, immunoprecipitation to mass spectrometry, and western blotting applications
• Stable for two years at -80°C

The tumor suppressor protein TP53 functions as a negative checkpoint regulator for abnormal cell signaling, and can induce cell cycle arrest, activate DNA repair proteins, or initiate apoptosis. Mutations in TP53 often result abnormal cell growth and are commonly found in many types of cancer. This lysate has been validated for TP53 expression and phosphorylation.

The BT-549 cell line is a human triple negative epithelial breast cancer cell line derived from mammary gland. The BT-549 cell lysate is provided as frozen lysate at a standard concentration of 1 mg/mL (500 mg per vial). Lysates are prepared in non-denaturing buffer without loading dyes or dissociating agents (i.e., no 2-mercaptoethanol or dithiothreitol has been added). For western blot analysis, heat lysate to 95°C for 5 minutes. Add reducing agent before heating if desired. The recommended loading volume per lane is 10–20 μL (10–20 µg).

Related products
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SureQuant RAS Isoform Panel (Absolute Quantitation)
SureQuant TP53 Panel (Absolute Quantitation)

ProFlex™ PCR System + Plastics Package, 1x96-well, 8-tube strips (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp 8-Tube Strips with Attached Domed Caps.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-µL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

Features include:
• Strip of eight tubes with attached domed caps that open and close independently to prevent contamination and evaporation
• Angled caps that provide efficient tube strip positioning in racks and thermal cycler blocks
• Etched A-H letter labeling on individual tubes that is visible when caps are open or closed
• Dual end tabs for proper labeling and easy handling
• Graduated 20-μL measuring markers on each tube for visual volume verification

ProFlex™ PCR System + Plastics Package, 2x384-well, EnduraPlates (Applied Biosystems™)

This ProFlex PCR System package includes a system plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

Proflex PCR System
The ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs:

• Interchange the 96-well 0.2-mL block with another block type if your throughput needs change
• Access the system remotely (and conveniently) through a mobile app
• Program the instrument in seconds with a simple-to-use touchscreen interface
• Simulate your old instrument with Thermal Simulation modes
• Optimize your PCR quickly and efficiently with better-than-gradient VeriFlex blocks

For detailed instrument information and specifications, see the ProFlex PCR System.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

Automated Thermal Cycler (ATC) + Plastics Package, 96-well, full-skirt EnduraPlates (Applied Biosystems™)

This Automated Thermal Cycler (ATC) package includes a thermal cycler plus MicroAmp EnduraPlates (full-skirt) and MicroAmp Clear Adhesive Films.

Automated Thermal Cycler (ATC)
For customers seeking to automate their PCR workflow, the Automated Thermal Cycler (ATC) provides the reliability and performance you’ve come to expect from Applied Biosystems instruments. Now in a small, easy-to-integrate, and compatible format, our Automated Thermal Cycler enables hands-free PCR results.

Space-saving design with our smallest thermal cycler footprint
• Flexible modular design helps save space on a robotic deck
• The separate control panel module provides flexibility when integrating on a robotic deck
• Choice of 10-cm, 1-m, and 3-m cables allows flexibility to locate the control module under the thermal cycler, under the deck, or up to 3 meteres away from the unit

Automated lid and cycler features
• The automated lid is compatible with programming for easy, hands-free operation with a liquid handler or plate stacker
• The heated cover slides forward to cover the plate nest, to help ensure thermal contact and eliminate evaporation
• The ATC is compatible with full-skirt or half-skirt plates

For detailed instrument information and specifications, see the Automated Thermal Cycler.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

Veriti™ Thermal Cycler + Plastics Package, 96-well, EnduraPlates (Applied Biosystems™)

This Veriti Thermal Cycler package includes a thermal cycler plus MicroAmp EnduraPlates and MicroAmp Clear Adhesive Films.

Veriti Thermal Cycler
The Veriti Thermal Cycler delivers the proven reliability of Applied Biosystems instruments with the throughput you need for large experiments.

Key features:
• Convenient color touchscreen speeds setup time and use
• Transfer protocols, back up protocols and system configurations with a USB port
• From a single, networked Veriti instrument, start a run on up to 12 Veriti systems
• Conveniently place instruments side-by-side due to front to back venting feature

For detailed instrument information and specifications, see the Veriti Thermal Cycler.

MicroAmp EnduraPlates
MicroAmp EnduraPlates offer excellent PCR or qPCR performance in formats developed to meet your experimental needs. They are a solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments. They are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

Key features:
• Optimized for performance with Applied Biosystems instruments
• Certified DNA-, RNAse-, and PCR inhibitor-free with in-process sampling tests
• Constructed to ANSI/SBS standard, well suited to high-throughput laboratories and robot handling
• Available in multiple colors for easy organization of laboratory assays
• Excellent mechanical stability and flatness for your complete workflow needs

MicroAmp Clear Adhesive Films
MicroAmp Clear Adhesive Films create a secure seal across an entire microplate to prevent evaporation and well-to-well contamination.

Key features:
• Adhesive provides tight seal over wells to prevent leaks and evaporation.
• Easy to apply and remove
• Available as Clear (economical and sticky) or Optical (ready for real-time PCR and non-tacky)

POROS™ GoPure™ MabCapture A Select Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

GoPure 0.5-cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS MabCapture A Select 50 µm resin in this GoPure column has a CaptureSelect protein A ligand immobilized to POROS backbone suitable for capture affinity chromatography..

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the GoPure family of pre-packed columns simplifies process scale-up and process modeling

GoPure 0.5-cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1-mL bed volume reduces sample requirements, and the recommended > 3-minute residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the GoPure 0.5-cmD Operating Instructions below.

Other POROS anion exchange resins, as well cation exchange resins, are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, GoPure Pre-Packed Columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 ml to 6.3 liters.

POROS™ GoPure™ Ethyl Pre-packed Column, 0.8 x 10 cm, 5 mL (Thermo Scientific™)

POROS GoPure 0.8 cmD pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.8 cmD x 10 cmL, with a nominal bed volume of 5 mL, providing a uniform screening platform
• Direct connection to standard chromatography systems
>• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• The 10-cm bed height and 5-mL bed volume allow initial process development at a bench scale
• Packed with POROS high performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Ethyl HIC resin in this GoPure column has a novel hydrophobic ethyl ligand, making it suited primarily for bind/elute purifications.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.