Genomics and proteomics search results

ZAP-70 Recombinant Human Protein

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. ...

Specifications

Gene ID
7535
Species
Human
Target Gene
zeta-chain (TCR) associated protein kinase
Gene Aliases
ZAP-70
Protein Form
Full Length
Recombinant
Cytoplasmic
Protein Family
Kinases & Inhibitors
Protein Subtype
Cytoplasmic Tyrosine Kinases
Accession Number
NP_001070
Molecular Weight
70.1 kDa
Research Category
Signal Transduction
System Compatibility
LanthaScreen® Substrate
LanthaScreen® Antibody
Z'-LYTE™
Omnia® Bulk Substrate
Validated Application
Kinase Assay
Protein Tag or Fusion
His Tag
Expression
Baculovirus
Kinase Family
SYK Kinase Family
Kinase Group
Tyrosine Kinases
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

P2782
7b1da7d40ea0961619e55cdc8954c08f_CmgtBuyProduct

Invitrogen™ ZAP-70 Antibody (1E7.2), Alexa Fluor® 488 conjugate, Molecular Probes™ (Invitrogen™)

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute. ...

Specifications

Reactivity
Human
Mouse
Validated Application
Flow Cytometry
Host
Mouse
Clonality
Monoclonal
Clone ID
1E7.2
Target Gene
ZAP-70
Gene ID
7535, 22637
Label or Dye
Alexa Fluor™ 488
Excitation⁄Emission (nm)
488⁄519
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate-Buffered Saline)
Comments
For use on any flow cytometer with a blue laser.
Research Category
Signal Transduction
Other Additives
BSA Stabilizer (Highly Purified Grade)
Shipping Condition
Wet Ice
Regulatory Statement
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Applications

MHZAP7020
307f917f56fd92bfbb1c19495a189298_CmgtBuyProduct

Invitrogen™ ZAP-70 Antibody (1E7.2), RPE conjugate, Molecular Probes™ (Invitrogen™)

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients. ...

Specifications

Reactivity
Human
Mouse
Validated Application
Flow Cytometry
Host
Mouse
Clonality
Monoclonal
Clone ID
1E7.2
Target Gene
ZAP-70
Gene ID
7535, 22637
Label or Dye
R-PE
Excitation⁄Emission (nm)
488⁄575
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate-Buffered Saline)
Comments
For use on any flow cytometer with a blue laser.
Research Category
Signal Transduction
Other Additives
BSA Stabilizer (Highly Purified Grade)
Shipping Condition
Wet Ice
Regulatory Statement
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Applications

MHZAP70044
9ab1ed12e0ac2d89e391f8f7a5851cb2_CmgtBuyProduct

Invitrogen™ ZAP-70 Antibody (1E7.2), Pacific Blue™ conjugate, Molecular Probes™ (Invitrogen™)

Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitatin ...

Specifications

Reactivity
Human
Mouse
Validated Application
Flow Cytometry
Host
Mouse
SubClass (Isotype)
IgG1
Clonality
Monoclonal
Clone ID
1E7.2
Antibody Fragment
Whole Antibody
Target Gene
ZAP-70
Target Molecule
Protein
Gene ID
7535, 22637
Label or Dye
Pacific Blue™
Excitation⁄Emission (nm)
405⁄455
Purification
Affinity Purified
Form
Liquid
Neutralizing Antibody
No
For Use With (Equipment)
Flow Cytometer
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Applications

MHZAP7028
922635f48b1b4e442d8f50addd411c83_CmgtBuyProduct

Pierce™ Phospho-Zap-70 pTyr315+319 Antibody (4H16L19), ABfinity™ Rabbit Monoclonal (Novex™)

This antibody is predicted to react with primate, bovine and Xenopus based on sequence homology. ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunofluorescence
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
4H16L19
Antibody Fragment
Whole Antibody
Target Gene
ZAP-70 [pY315/pY319]
Gene ID
7535
Accession Number
P43403
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Affinity Purified
Concentration
0.5 mg⁄ml
Color
Unconjugated
Neutralizing Antibody
No
For Use With (Equipment)
Fluorescence Microscope
Flow Cytometer
Confocal Microscope
Protein Family
Kinases & Inhibitors
Volume (Metric)
200 µl
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

700177
58c995486fc93a242b987640b692db8c_CmgtBuyProduct

Novex™ Phospho-ZAP-70 pTyr292 Antibody (Novex™)

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial ste ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ZAP-70 [pY292]
Gene ID
7535
Label or Dye
Unconjugated
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44230G
c88c5f0fba58ac8477fff5b7aaa0c51f_CmgtBuyProduct

Novex™ Phospho-ZAP-70 pTyr315 / pTyr319 Antibody (Novex™)

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial ste ...

Specifications

Reactivity
Human
Validated Application
Western Blot
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
ZAP-70 [pYpY315/319]
Gene ID
7535
Label or Dye
Unconjugated
Target Modification State
Phospho
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

44232G
8b79fb560d151934039af4c9cc6d501f_CmgtBuyProduct

Novex™ ZAP70 Antibody (ZAP-70-6F7) (Novex™)

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial ste ...

Specifications

Reactivity
Human
Mouse
Validated Application
ELISA
Western Blot
Immunoprecipitation
Host
Mouse
Clonality
Monoclonal
Clone ID
ZAP-70-6F7
Target Gene
ZAP-70
Gene ID
22637, 7535
Label or Dye
Unconjugated
Purification
Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

130600
a30fbd587840e409672e829a13f79735_CmgtBuyProduct

Pierce™ Phospho-Zap-70 pTyr493 Antibody (Thermo Scientific™)

It is not recommended to aliquot this antibody. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA517778
6537553bc313782667fd58afe8ee65b8_CmgtBuyProduct

Pierce™ Phospho-Zap-70 pTyr319+Syk pTyr352 Antibody (R.658.6) (Thermo Scientific™)

It is not recommended to aliquot this antibody. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
MA515095
38a116a5959906c46a206a74dce19719_CmgtBuyProduct

Pierce™ Phospho-Zap-70 pTyr319+Syk pTyr352 Antibody (Thermo Scientific™)

It is not recommended to aliquot this antibody. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA517815
677b8d135037a0f63a6a9b354dad669a_CmgtBuyProduct

Pierce™ ZAP-70 Antibody (2F3.2) (Thermo Scientific™)

MA5-12624 targets ZAP-70 in IHC (P) and WB applications and shows reactivity with Human samples. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
MA512624
0887ae3e282a5b955a1405cd48016ef5_CmgtBuyProduct

Pierce™ ZAP-70 Antibody (Thermo Scientific™)

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggeste ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA532644
ac48eef9dfae1e3048454d067d19f041_CmgtBuyProduct

LanthaScreen® Tb-PY20 Antibody Kit

A general reagent to monitor tyrosine kinase activity, the pY20 antibody binds to phosphorylated tyrosines within a peptide or physiological protein substrate. ...

Specifications

Format
384-well plate
Readout
End Point
Technique
TR-FRET
Label or Dye
FITC (Fluorescein)
Tb (Terbium)
Target Enzyme
Tyrosine Kinases
Validated Kinases
ABL1, ABL2, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, ERBB2, ERBB4, FER, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1, FLT3, FLT4, FRK, FYN, HCK, IGF1R, INSR, KDR, KIT, LCK, LTK, LYNA, LYNB, MATK, MERTK, MET, MST1R, MUSK, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PTK2, PTK2B, PTK6, RET, ROS1, SRC, SRCN1, SRMS, SYK, TEC, TEK, TNK2, TTK, TYRO3, YES1, ZAP70
Antibody Molecular Weight
150000 Da
Excitation⁄Emission (nm)
340⁄490, 520
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV3553
e83e711838c5a3c1af253a2b725376aa_CmgtBuyProduct

Novex™ ZAP70 Antibody (1E7.2) (Novex™;Thermo Scientific™)

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial ste ...

Specifications

Reactivity
Human
Mouse
Validated Application
Flow Cytometry
Immunoprecipitation
Western Blot
Host
Mouse
Clonality
Monoclonal
Clone ID
1E7.2
Target Gene
ZAP-70
Gene ID
7535
Label or Dye
Unconjugated
Purification
Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

376500
127e72c8c54dc9faa155712a487bddee_CmgtBuyProduct