Citations & References

A novel progranulin mutation associated with variable clinical presentation and tau, TDP43 and alpha-synuclein pathology.

  • Authors: Leverenz JB; Yu CE; Montine TJ; Steinbart E; Bekris LM; Zabetian C; Kwong LK; Lee VM; Schellenberg GD; Bird TD
  • Journal: Brain : A Journal of Neurology 2007 PT 5:1360-1374

Citations & References

Immunohistochemistry for PMS2 and MSH6 alone can replace a four antibody panel for mismatch repair deficiency screening in colorectal adenocarcinoma.

  • Authors: Hall G, Clarkson A, Shi A, Langford E, Leung H, Eckstein RP, Gill AJ,
  • Journal: Pathology (2010) 42:409-413
  • PubMed ID: 20632815
Catalog #
  • 180443(Discontinued)

Citations & References

Assessment of Morphology, Antigenicity, and Nucleic Acid Integrity for Diagnostic Thyroid Pathology Using Formalin Substitute Fixatives

  • Authors: Lassalle, S; Hofman, V; Marius, I; Gavric-Tanga, V; Brest, P; Havet, K; Butori, C; Selva, E; Santini, J; Mograbi, B; Hofman, P
  • Journal: THYROID 2009 11:1239-1248

Citations & References

The role of CD146 (Mel-CAM) in biology and pathology.

  • Authors: Shih IM
  • Journal: J Pathol 1999; (189):1 4-11
  • PubMed ID: 10451481
Catalog #
  • 10115R012AB(Discontinued)
  • 10115RP02AB(Discontinued)

Citations & References

Diagnosis of NUT Midline Carcinoma Using a NUT-specific Monoclonal Antibody

  • Authors: Haack, H; Johnson, LA; Fry, CJ; Crosby, K; Polakiewicz, RD; Stelow, EB; Hong, SM; Schwartz, BE; Cameron, MJ; Rubin, MA; Chang, MC; Aster, JC; French, CA
  • Journal: AMERICAN JOURNAL OF SURGICAL PATHOLOGY 2009 7:984-991

Citations & References

Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis.

  • Authors: Mustafa T; Wiker HG; Mfinanga SG; Morkve O; Sviland L
  • Journal: Modern Pathology : An Official Journal of the United States and Canadian Academy of Pathology, Inc 2007 1:1606-1614

Citations & References

Characterization of R132H Mutation-specific IDH1 Antibody Binding in Brain Tumors

  • Authors: Capper, D; Wessert, S; Balss, J; Habel, A; Meyer, J; Jaeger, D; Ackermann, U; Tessmer, C; Korshunov, A; Zentgraf, H; Hartmann, C; von Deimling, A
  • Journal: BRAIN PATHOLOGY 2010 1:245-254

Citations & References

Pathology of fatal West Nile virus infections in native and exotic birds during the 1999 outbreak in New York City, New York.

  • Authors: Steele KE; Linn MJ; Schoepp RJ; Komar N; Geisbert TW; Manduca RM; Calle PP; Raphael BL; Clippinger TL; Larsen T; Smith J; Lanciotti RS; Panella NA; McNamara TS
  • Journal: Veterinary Pathology 2000 3:208-224

Product FAQ

What is the recommended protocol for removal of endogenous peroxidase activity?

Answer

Procedure 1 (Use for paraffin-embedded sections, not for frozen sections):
Prepare peroxidase blocking solution by adding one part 30% hydrogen peroxide to nine parts methanol. Submerge slides in this solution for 10 minutes. Wash three times with PBS.

Procedure 2 (May be used with frozen or paraffin-embedded sections):
Treat slides with Peroxo-block (Cat. No. 00-2015) for 45 seconds. Wash immediately.

The removal of endogenous peroxidases can be performed before or after antigen retrieval or before and after the primary antibody incubation step.

Answer Id: E4910x

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Product FAQ

What protocol is recommended for removal of residual biotin, avidin or streptavidin activity from tissue sections?

Answer

For removal of residual biotin: Incubate section with the avidin solution from the Blocking kit (Cat. No. 00-4303). Wash. Incubate with the d-biotin solution from the kit. Wash thoroughly.

For removal of residual Avidin or Streptavidin: Incubate section with the d-Biotin solution from the Blocking kit (Cat. No. 00-4303). Wash thoroughly.

Removal of residual biotin, avidin or streptavidin activity should occur immediately after normal serum blocking and before primary antibody incubation. This blocks endogenous biotin exposed after the antigen retrieval procedure as well as any endogenous biotin present in normal serum.

Answer Id: E4912x

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Product FAQ

Is antigen retrieval needed for staining frozen tissue sections?

Answer

Antigen retrieval may be needed if cryosections are post-fixed in formalin, PFA, etc., which induce epitope-masking protein cross-links. Antigen retrieval is usually not needed if post-fixation is done in methanol, acetone, etc. You can also expose hidden epitopes with a pre-staining permeabilization step.  This facilitates antibody penetration, and it can efficiently unmask some antigens.

Answer Id: E4904x

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Product Literature

Life in the Lab - Technology Propelling Breakthroughs (North America)

Citations & References

Activated microglia in cortex of mouse models of mucopolysaccharidoses I and IIIB.

  • Authors: Ohmi K, Greenberg DS, Rajavel KS, Ryazantsev S, Li HH, Neufeld EF
  • Journal: Proc Natl Acad Sci U S A (2003) 100:1902-1907
  • PubMed ID: 12576554
Catalog #
  • I21410(Discontinued)

Citations & References

Choroideremia: Analysis of the retina from a female symptomatic carrier

  • Authors: Bonilha, VL; Trzupek, KM; Li, Y; Francis, PJ; Holly, JG; Rayborn, ME; Smaoui, N; Weleber, RG
  • Journal: OPHTHALMIC GENETICS 2008 3:99-110

Product Literature

Life in the Lab - Technology Propelling Breakthroughs (Singapore and Southeast Asia)