Documents & Support

Regulatory requirements vary by product. Please see product-specific literature to determine product use.

Why is the ADD DISSOCIATION CURVE button disabled in the Relative Quantification Plate Assay in the SDS software on the ABI PRISM 7000, Applied Biosystems 7300, 7500, 7500 Fast and the 7900HT Systems? Product FAQ

Answer

The older versions of the software did not include this feature for all assay types. For the 7500 and 7500fast instruments, the latest software 2.0.5 allows a melt curve to be added to the PCR experiment. The other instruments/software require a separate program for melt curves. To run a Dissociation Curve after running a Relative Quantification Plate (ddCt) Assay, you will need to open a new assay from File -> New, select DISSOCIATION from the assay pull-down menu and run the Dissociation Curve as a separate assay. This will generate a separate SDS file for the Dissociation Run.

Answer Id:: E2504

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Why have my primary cells started to slow in growth or stopped growing? Product FAQ

Answer

Primary cells are not immortalized and undergo a limited number of population doublings. Each cell type has a different maximum population doubling number that is found on the certificate of analysis for the assigned lot number.

Answer Id:: E12034

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What is the maximum thermal output for the ABI PRISM 7000 Sequence Detection System in BTU/h? Product FAQ

Answer

The maximum thermal output for the ABI PRISM 7000 Sequence Detection System is 2285 BTU/h (~675W).

Answer Id:: E2505

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Why aren't my primary cells attaching to the surface of the plate/flask? Product FAQ

Answer

Be sure to use the Coating Matrix Kit if you are using the Animal Origin-Free (AOF) supplementation for your culture. There are no attachment factors in the AOF supplements, and Coating Matrix Kit is required for cells to adhere properly.

Answer Id:: E12035

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Why must all gel buffer be removed from the gel prior to staining with Pro-Q Emerald 300/488 Glycoprotein stains? Product FAQ

Answer

If the gel buffer contains Tris, glycine or any other component that has a primary amine in its structure, the primary amine will react directly with the aldehydes/ketones formed upon periodic acid oxidation, effectively capping all reactive sites and leaving no sites for the Pro-Q Emerald dye reagent to bind to.

Answer Id:: E11204

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Where can I find a protocol to perform Fast TaqMan Real-Time PCR using the 384-well block on the Applied Biosystems 7900HT? Product FAQ

Answer

For detailed instructions on performing Fast TaqMan Real-Time PCR using the 384-well block on Applied Biosystems 7900HT, please refer to User Bulletin for Performing Fast TaqMan Gene Quantitation using 384 well plates on the Applied Biosytems 7900HT Fast-Real-Time PCR System with the 384 well Block Module. (P/N 4369584)

Answer Id:: E2506

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Can I use BSA as a blocking agent during isolation of His-tagged proteins using Dynabeads His-Tag Isolation and Pulldown magnetic beads? Product FAQ

Answer

BSA would only prevent non-specific adsorption to the surface. Also, if the BSA binds to the chelator, it will block His-tag binding. Instead, we recommend that you use 0.01% Tween 20 detergent instead.

Answer Id:: E6052

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Which GCOS features are not supported in Command Console Software? Product FAQ

Answer

Publishing and publish databases will not be supported. However, customers can leverage the flexibility of the file-based system to populate their own library information management systems (LIMS).
CHP data will be generated by separate Affymetrix and third-party applications, including Expression Console Software and Genotyping Console Software.
GeneArray 2500 and Fluidics Station FS400 instruments are not supported by Command Console Software.
Windows 2000 operating system is not supported.

Answer Id:: E14228

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What is the correct fluidics script for the Clariom S (human, mouse and rat) array? Product FAQ

Answer

The Clariom S array is a 400 format, the correct script is FS450_0007.

Answer Id:: E14311

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Should a high copy number plasmid be used with Expressway system? Product FAQ

Answer

Yes. We recommend starting out with a high copy number plasmid. This way a researcher can go right from a Miniprep kit directly into the Expressway reaction. Many of the pET vectors are low copy, and need to be concentrated before being used in an in vitro expression system.

Answer Id:: E3892

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Can I use your lipid reagents to co-transfect plasmids? Product FAQ

Answer

Yes. The standard transfection protocol may be followed by keeping the total amount of DNA in the mixture constant. That is, if your protocol requires 1 µg plasmid, use 0.5 µg of each of two co-transfected plasmids, or 0.25 µg of each of 4 co-transfected plasmids. When performing co-transfections to introduce a selectable marker on a different plasmid, we recommend using a 3:1 to 10:1 molar excess of the plasmid of interest over the selectable plasmid to ensure that the plasmid of interest is present with the selectable plasmid.

Answer Id:: E8970

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Can I transfect my primary cells? Product FAQ

Answer

Yes, we strongly recommended that you contact our Molecular Biology scientists at techsupport@thermofisher.com to help you choose the best transfection reagent option, as primary cells tend to be very sensitive.

Answer Id:: E12036

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What is the TAL workflow? Product FAQ

Answer

- Design Invitrogen GeneArt TALs for target DNA, and clone into Gateway entry or destination vectors
- In vitro validation of TALs (optional)
- Transfect cells with TAL expression vectors
- TAL-mediated target cleavage
- Cleavage analysis using Invitrogen GeneArt Genomic Cleavage Detection Kit

Answer Id:: E10207

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How do I change/customize labels on the plot? Product FAQ

Answer

Labels on the plot are turned on by default.
1. In the Results screen, click Plot Settings to display the plot settings selector screen.
2. In the Label section, click Options.
3. Select parameters to display in the plot heading. Possible parameters include:
- Area (Default)
- Height (Default)
- Size (Default)
- Data Point (Default)
- Comments

Answer Id:: E15996

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Why must residual periodic acid be removed from the gel prior to staining with Pro-Q Emerald 300/488 Glycoprotein stains? Product FAQ

Answer

Residual periodic acid must be removed from the gel prior to staining with Pro-Q Emerald 300/488 Glycoprotein stains so as to limit oxidation/decomposition of the Pro-Q Emerald dye.

Answer Id:: E11205

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