Regulatory requirements vary by product. Please see product-specific literature to determine product use.

Product FAQ

How should I store Dynabeads magnetic beads?

Answer

When stored in unopened vials at 2-8 degrees C, Dynabeads magnetic beads are stable until the expiration date printed on the label. Freezing or drying of the Dynabeads magnetic beads is not recommended. Dynabeads magnetic beads should be fully resuspended before use.

Answer Id: E4866

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Product FAQ

What is the function of the E-Editor 2.0 software?

Answer

The E-Editor 2.0 software allows you to quickly reconfigure digital images of E-Gel 48, E-Gel 96, E-PAGE 48 and E-PAGE 96 gel results for analysis and documentation. You can capture an image of the gel and then use the E-Editor 2.0 software to:
-Align and arrange the lanes in the image to any 48, 96, or 384 image
-Save the reconfigured image for further analysis
-Copy and paste selected lanes or the entire image into other applications for printing, saving, e-mailing, and/or publishing on the Web.

The E-Editor 2.0 does not take perform densitometry analysis from your gel images. The E-Editor 2.0 can be downloaded for free.

Answer Id: E4868

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Product FAQ

What are some troubleshooting hints if there is high background after Coomassie staining of E-PAGE?

Answer

If you observe high background after Coomassie staining/destaining, here are some things to check:

It is very important to use 0.015% Coomassie R-250. Even using 0.03% will make Coomassie destaining more difficult.

The gel shouldn't be left in stain for more than 1 hour.

Increasing the destain time may reduce background.

Warming the gel and destain solution is also very important for best results.

Answer Id: E4869

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Product FAQ

Can I use my own blocking reagent or antibody incubation solution with the iBind Flex Western System?

Answer

We do not recommend using your own solutions. The iBind Flex Solution and iBind Flex Fluorescent Detection (FD) Solution have specific viscosity and are optimized for the sequential lateral flow that is the principle of the iBind Flex Western device. We cannot guarantee the performance with any other solutions.

Answer Id: E10819

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Product FAQ

How can I reduce background bands in my Western blot?

Answer

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Answer Id: E3166

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Product FAQ

What transfer buffer do I use to blot E-PAGE gels in a semi-wet transfer protocol (with the XCell II blot module)?

Answer

NuPAGE Transfer Buffer with 10% methanol provides optimal transfer of E-PAGE gels in the XCell II Blot Module. The NuPAGE Antioxidant is used in the transfer buffer for blotting reduced proteins and prevents the proteins from re-oxidizing. The Tris-Glycine Transfer Buffer has not been tested and it is not known as to what the transfer efficiencies would be like.

Answer Id: E4870

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Product FAQ

Are the PureLink 96/384 systems compatible with the Qiagen robotic system?

Answer

The original QiaRobot does not allow modifications to its protocol; however, more recent models are more flexible. Contact Technical Support to learn about robots that are compatible with the system.

Answer Id: E3803

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Product FAQ

At what temperature should my AmpliTaq Gold PCR Master Mix (Cat. No. 4318739) be stored?

Answer

AmpliTaq Gold PCR Master Mix should be stored at 4 degrees C.

Answer Id: E1589

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Product FAQ

What is the recommended protocol for removal of endogenous peroxidase activity?

Answer

Procedure 1 (Use for FFPE, but not frozen sections):
Prepare peroxidase blocking solution by adding one part of 30% hydrogen peroxide to nine parts of methanol. Submerge the slides in this solution for 10 min. Wash three times with PBS.

Procedure 2 (May be used with frozen or FFPE sections):
Treat slides with Peroxidase Suppressor (Cat. No. 35000) for 15-30 min at room temperature. If you wish, sections can be treated with Lab Vision products (Cat. Nos. TA-060-HP or TA-125-HP) instead. These alternative products also inactivate endogenous peroxidase activity in tissue sections and other samples. Wash immediately.

The removal of endogenous peroxidases can be performed before or after antigen retrieval or before and after the primary antibody incubation step.

Answer Id: E4910x

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Product FAQ

What are pros and cons of using DETACHaBEAD Kits?

Answer

The major advantage using DETACHaBEAD Kits is to obtain both antibody-and bead-free cells after isolation. In addition, the purity and viability is high. Unfortunately, DETACHaBEAD Kits are available for only a few human cell types-the release cannot be made generic, since there is one unique DETACHaBEAD for each product. To obtain the optimal yield, the release step needs to be performed at room temperature for about 45 min. DETACHaBEAD reagents will interfere with the FACS readings of the cells after isolation, so it is important to wash the cells after release according to the protocol.

Answer Id: E12103

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Product FAQ

What is the recommended amount of time to immerse the membrane in iBind Flex Solution/iBind Flex Fluorescent Detection (FD) Solution prior to assembly in the iBind Flex Western device?

Answer

It is sufficient to block the membrane in iBind Flex Solution/ iBind Flex Fluorescent Detection (FD) Solution for the time prior to making antibody dilutions. It is not necessary to block the membrane overnight in the blocking solution. The membrane will be subjected to additional blocking in the iBind Solution/ iBind Flex Fluorescent Detection (FD) Solution before primary antibody reaches the membrane.

Answer Id: E10820

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Product FAQ

Will ultra thin wall (UTW) vessels automatically speed up reactions on my conventional thermal cycler?

Answer

To realize the time savings of the UTW plastics on conventional thermal cyclers, optimization of the protocol is required. This typically involves reducing hold times and changing the actual hold temperatures.

Answer Id: E8701

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Product FAQ

I have a red blinking light on the Applied Biosystems 310 Genetic Analyzer. What does it mean and how can I fix it?

Answer

A blinking red light on the instrument may indicate that a hardware component has failed or a possible communication disruption has occurred between the instrument and computer. Completely shut down the instrument, wait for 2 minutes, and then start up in the order documented in Applied Biosystems 310 Genetic Analyzer: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/310%20Start%20Up%20Procedures.pdf) or the Applied Biosystems 310 Genetic Analyzer User Guide (http://tools.thermofisher.com/content/sfs/manuals/cms_041158.pdf).
If the instrument goes from a flashing amber light to red light, one or more of the hardware components has failed the hardware diagnostic and a service call should be opened up.

Answer Id: E12681

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Product FAQ

I am observing speckling in my gel stained with Pro-Q Emerald 300/488 Glycoprotein Gel Stain, especially near the edges. What is causing this?

Answer

Speckling of Pro-Q Emerald dye, especially with Pro-Q Emerald 300 stain, can occur as the Pro-Q Emerald dye ages, due to self-aggregation of the dye over time. Speckles can also form due to dye binding to contaminants from the staining container, solutions, or particles from the air or gloves. Non-dye speckles can also show up in the image from auto-fluorescent particles of dust, hair, glove powder, or clothing lint that falls on the gel or surface of the glass imaging plate. The better the imager is at focusing on surface features of the gel, the more speckles that are going to be visible.

To minimize the formation of speckles and other background debris, follow clean laboratory practices, use ultrapure water of >18 megohm-cm resistance to prepare solutions, rinse gloves in water to remove powder residue before touching gels, use lint-free wipes and wear a lab coat or avoid wearing clothing that generates a lot of lint, always rinse the staining container with ethanol and wipe out any residual dye before staining another gel, and always rinse and wipe down the glass imaging surface with ethanol and water before placing your gel down. Once speckles have been deposited on the gel, it is not possible to wash them off. When analyzing amounts of glycoprotein near the limit of detection, we advise running samples in the middle lanes of the gel.

Speckles will show up as sharp, tall spikes on 3D renditions of gel images. These spikes look distinct from 3D renditions of protein spots or bands. Some image analysis software packages have de-speckling algorithms that can easily identify and remove this type of pixelated noise.

Answer Id: E11301

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Product FAQ

What type of DNA fragments can be cloned into the pJET1.2/blunt vector?

Answer

Any DNA fragment (either blunt- or sticky-end) that is 6 bp to 10 kb long and generated from PCR or restriction digestion, can be successfully cloned using the kit.

Answer Id: E8846

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