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Regulatory requirements vary by product. Please see product-specific literature to determine product use.

What are the key features of a TaqMan MGB probe? Product FAQ

Answer

TaqMan MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes.  For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box.

Answer Id:: E1371

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What are the major improvements in the design of the arrays in the GeneChip Mouse Expression Set 430? Product FAQ

Answer

The Mouse Expression Set 430 incorporates the same expertise that was utilized for the design and performance of the GeneChip Human Genome U133 Set. For the design, genomic sequences were used to verify sequence selection, orientation, and the quality of sequence clustering. Additionally, clustering information from UniGene, build 107, was used with primary sequences and annotation information combined from a large variety of public databases to provide a more complete and accurate starting sequence data set.

Answer Id:: E13720

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Why are lentiviruses able to transduce non-dividing cells when other viruses cannot? Product FAQ

Answer

Lentiviruses gain entry into the host cell nucleus by means of an active import mechanism, using the host cell machinery. They enter the host cell nucleus and immediately integrate into the host cell genome. Hence, they are able to transduce both dividing and non-dividing cells. Retroviruses, on the other hand, use a passive entry mechanism and require a round of cell division before the virus can enter the host cell nucleus.

Answer Id:: E6393

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How has the GeneChip Mouse Expression Set 430 design and performance been tested? Product FAQ

Answer

Briefly, during development, a tissue (11 tissues) and cell line (4 cell lines) panel was hybridized to both Mouse 430 and MG-U74v2 arrays. This sample set was used to evaluate tissue-specific expression patterns and for studies to compare data from the previous and current designs, including analysis of variance. A set of 30 prelabeled spikes derived from clones of mouse genes was used to evaluate sensitivity. Approximately 30 transcripts were evaluated both by array and by real-time quantitative PCR (TaqMan) to evaluate performance.

Answer Id:: E13721

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What is Polybrene and how does it facilitate lentiviral transduction? Product FAQ

Answer

Polybrene (hexadimethrine bromide) is a cationic polymer used to increase the efficiency of infection of certain cells with virus particles in cell culture. Polybrene acts by neutralizing the charge repulsion between the virions and cell surface.

Answer Id:: E6394

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With my Applied Biosystems 3130/3130xl Genetic Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it? Product FAQ

Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id:: E12716

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What is the nature of specificity of the probe sets included in the GeneChip Mouse Expression Set 430? Product FAQ

Answer

The probes on the Mouse Set 430 are designed to detect the anti-sense strand of the gene of interest, and these probe sets are annotated with the "_at" extension. The primary goal in probe selection is to select a probe set unique to a single transcript or common among multiple transcripts from the same gene.

Often it is not possible to select a probe set that is unique to a single transcript because multiple transcript variants share common sequence. Such probe sets have the "_s" extension. Occasionally, it is not possible to select a unique or shared probe set. Such probe sets have the "_x" extension.

For more information, please review the "_s" and "_x" probe set descriptions. Please note: There are no "_f", "_g", "_r", "_i", or "_n" probe sets in the Mouse Expression Set 430 design. This is because the non-unique probe set types were simplified and adjusted to account for improvements to the probe selection rules.

Answer Id:: E13722

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Can Platinum SuperFi DNA Polymerase amplify AT-rich targets? Product FAQ

Answer

Yes. To improve amplification of AT-rich targets, we recommend reducing the extension temperature to 68 degrees C or add 5-15 mM Tetramethylammonium Chloride (TMAC).

Answer Id:: E13106

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I am seeing a very wavy and uneven dye front with my samples. Can you please help me troubleshoot? Product FAQ

Answer

This could be due to a gel polymerization issue combined with incorrect sample preparation (final sample dilution less than 1X). Please try a different lot of the same gel and make sure that the sample is correctly prepared.

Answer Id:: E10585

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How many SNPs and Copy Number Probes are on the SNP 5.0 array? Product FAQ

Answer

500,568 SNPs on the SNP 5.0 array can be accessed utilizing Genotyping Console 2.1. In addition, the SNP 5.0 array contains 420,000 non-polymorphic copy number probes.

Answer Id:: E13746

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I am getting small and large colonies after transformation of my lentiviral construct into Stbl3 E.coli. Which colonies should I pick? Product FAQ

Answer

We recommend picking the smaller colonies. Large colonies are usually the result of recombination events that result in loss of some part of the plasmid, conferring a growth advantage on the cells.

Answer Id:: E6395

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Do you have a manual of install/uninstall procedures for GeneMapper Software v6? Product FAQ

Answer

Please download this PDF (https://downloads.thermofisher.com/Instrument_Software/CE/GeneMapper/Full_v6.0/100079198_MNL_GENEMAPPER_V_6_INSTALLATION.pdf) and follow the directions for install/uninstall GeneMapper Software v6.

Answer Id:: E16579

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How many filters does Multiskan FC hold? Product FAQ

Answer

The filter wheel holds up to 8 filters.

Answer Id:: E16710

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My array is stuck in the pump on my Applied Biosystems 3130/3130xl Genetic Analyzer, even after loosening the knob. What can I do? Product FAQ

Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id:: E12717

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I’m seeing RACE PCR artifacts in my GeneRacer experiment. What am I doing wrong? Product FAQ

Answer

RACE PCR artifacts or nonspecific PCR bands can result from one or more of the following:

-Nonspecific binding of GSPs to other cDNAs resulting in the amplification of unrelated products as well as desired products.
-Nonspecific binding of GeneRacer primers to cDNA resulting in PCR products with GeneRacer primer sequence on both ends of the PCR product.
-RNA degradation.
-Contamination of PCR tubes or reagents.
Note: Artifacts usually result from less than optimal PCR conditions and can be identified in negative control PCR.

Answer Id:: E7334

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