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Regulatory requirements vary by product. Please see product-specific literature to determine product use.

What shoud I do if R1, R2, R3, and/or R4 fails at Raw Traces and the screen turns red during the last step of initialization of my Ion PGM System? Product FAQ

Answer

The pH of the nucleotides may be out of range or there may have been a minor problem during measurement. Press the 'Start' button and restart the measurement. If it passes, continue to start the run. If an error appears again, please note the pH of R1, R2, R3, R4, as well as the error message, and contact Technical Support.

Answer Id:: EJP01165

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What should I do if an error message "calibration FAILED" appears during initialization of my Ion PGM System? Product FAQ

Answer

Open the chip clamp and check for any leaks. If you see solution leaking, please remove the chip and clear the solution with a Kimwipe. DO NOT wipe or scrub as the socket could be damaged. Clean the socket by gently touching it with a damp Kimwipe. Make sure the socket is dry and replace the chip. Press the 'Calibration' button to recalibrate the chip. If it fails again, change the chip to a different one. Also, if you are using an Ion 316 Chip v2 on a v3.4 or older instrument, the instrument will not recognize the chip.

Answer Id:: EJP01171

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Can I use the same oil between the Ion PGM OT2 200 Kit and the Ion PGM OT2 400 Kit? Product FAQ

Answer

No, you may not. Please exchange the oil when switching between different kits. Contact Technical Support for any needed assistance when switching kits.

Answer Id:: EJP01176

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Can I perform two 850 flow runs with an Ion 314 Chip? Product FAQ

Answer

You may, but W2 may run out in the middle of the second run. However, you can collect the data right before W2 runs out and reanalyze it after the run finishes. If you want complete data, please perform only one run per initialization.

Answer Id:: EJP01182

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What shoud I do if an alert "No Connect FTP Server" appears on the Ion PGM touchscreen? Product FAQ

Answer

The Ion PGM System and Torrent Server may not be connected. Please shut down the system and server and reboot them. The server may enter a system check after the reboot. Since the system check will take 3-4 hours, you can avoid it by pressing the 'c' button on the keyboard.

If there is a reason you cannot reboot the system, you can run the Ion PGM System without connection to the server. The runs will be saved inside the system. For 200 bp sequencing, this many runs can be saved inside the system:
314 chip: 40 runs
316 chip: 6 runs
318 chip: 5 runs

The data will automatically transfer to the server after connection.

Answer Id:: EJP01167

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How long can I wait until I start the run after initialization of my Ion PGM System? Product FAQ

Answer

If you are using the Ion PGM™ 200 Sequencing Kit v2, you can perform a run within 27 hours after initialization. If you are going to perform two runs, make sure the second one starts within 27 hours.

If you are using the Ion PGM™ 200 Sequencing Kit, the limit is shorter; you will need to perform a run within 10 hours.

Answer Id:: EJP01174

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What should I do if the Recovery Solution does not come out from Ion OneTouch DL? Product FAQ

Answer

The Ion OneTouch DL cannot be repaired. However, you may order an Ion OneTouch DL Upgrade Kit and exchange some parts which may fix the problem.

Answer Id:: EJP01180

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What is the picture on the touchscreen during an Ion Proton run that looks like a bubble moving? Product FAQ

Answer

The touchscreen shows the voltage measured on the chip and there will be some contrast according the signal intensity which may look like bubbles.

Answer Id:: EJP01173

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If I increase the final concentration of my SYPRO Orange or SYPRO Red staining solution above 1X, can I increase the signal of my stained proteins? Product FAQ

Answer

No. Dye concentrations higher than 1X do not give better detection. Instead, background fluorescence increases and, as the dye concentration increases, the dye becomes self-quenching and the signal actually decreases.

Answer Id:: E11151

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Can you suggest a positive control to use with the InVision His-Tag In-Gel Stain? Product FAQ

Answer

The BenchMark His-tagged Protein Standard is ideal for use as a positive control for the InVision His-tag In-gel Stain. The standard is formulated to allow simultaneous detection of standard proteins and His-tagged fusion proteins. The BenchMark His-tagged Protein Standard is included in the InVision His-tag In-Gel Staining Kit (Cat. No. LC6033).

Answer Id:: E11175

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What are the special features of the Lumio Green Detection kit? Product FAQ

Answer

The Lumio Green Detection kit is a sensitive and highly specific kit for labeling Lumio fusion proteins prior to electrophoresis. It enables immediate visualization of Lumio fusion protein bands directly in a polyacrylamide gel.

Answer Id:: E11181

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How can I visualize my Lumio-tagged protein after staining with Lumio Green Detection Reagent? Product FAQ

Answer

To visualize Lumio-tagged protein bands after staining, you will need one of the following:

- UV transilluminator (302 nm) equipped with a standard video camera, CCD (Charged Couple Device) camera, or a cooled CCD camera with ethidium bromide filter or SYBR Green filter. Note: You can use a 365 nm UV transilluminator, but you may have to expose the gel for a longer time, as the sensitivity is lower than using 302 nm UV transillumination.
- Laser-based scanner with a laser line that falls within the excitation maxima of the stain (500 nm), and a 535 nm long pass filter or a band pass filter centered near the emission maxima of 535 nm. The sensitivity of detection is higher with laser-based scanners equipped with appropriate filters than with UV transillumination.

Answer Id:: E11187

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What is the sensitivity of the Thermo Scientific 6xHis Protein Tag Stain Reagent Set? Product FAQ

Answer

The Thermo Scientific 6xHis Protein Tag Stain Reagent Set can detect as low as 0.2 µg of a 35 kDa (5.7 pmol) His-tagged protein per band using a CCD camera, and as low as 2 µg (57 pmol) of the His-tagged protein per band with a UV transilluminator. Detection requires illumination of the stained gel with UV-light at a wavelength in the range 280-310 nm.

Answer Id:: E11193

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Can I use other molecular weight standards with the Pro-Q Diamond Phosphoprotein stain? Product FAQ

Answer

Other known phosphoproteins can be used as positive control standards for the Pro-Q Diamond Phosphoprotein stain. Ovalbumin, in the Protein Molecular Weight Standards Reagent (Cat. No. P6649) is a phosphoprotein. None of the proteins in the Mark12, Invitrogen Sharp, SeeBlue or SeeBlue Plus2 standards is a phosphoprotein that could be used as a positive control with the Pro-Q Diamond Phosphoprotein stain.

Answer Id:: E11199

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Can western blotting filter paper be used with the Thermo Scientific Power Stainer? Product FAQ

Answer

No. The Mini and Midi Gel Pads (8 layers per pad) are required to be used with the Thermo Scientific Power Stainer, Power Stain Solution and Destain Solution. The gel pads act as reservoirs for Power Stain Solution and Destain Solution. Standard western blotting filter paper will cause uneven staining and patchy background.

Answer Id:: E11219

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