Regulatory requirements vary by product. Please see product-specific literature to determine product use.

Product FAQ

What dyes are present in Phusion Green and Phire Green buffers?

Answer

Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.

Answer Id: E17121

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Product FAQ

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Answer

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Answer Id: E17122

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Product FAQ

Why is the lot number on the Zeocin Selection Reagent (Cat. No. R25001) vial different from the one on the package?

Answer

Zeocin Selection Reagent (Cat. No. R25001) contains 1 g of Zeocin Selection Antibiotic supplied as a 100 mg/mL solution (Part No. 450430) in 8 x 1.25 mL vials. Part No. 450430 is associated with a specific lot number that is designated by our manufacturing tracking system, and this is the lot number that is listed on the package. The COA is also created based on this package part number and lot number.

Each of the 8 vials in turn has a Part No. 460509 that is associated with a different lot number designated by our manufacturing tracking system. This lot number is mainly used for our internal inventory purpose.

Answer Id: E17123

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Product FAQ

What advantage do Pierce Magnetic Agarose beads have over Dynabeads or Pierce Magnetic Beads?

Answer

Pierce Magentic Agarose Beads have a much higher binding capacity.

Answer Id: E16148

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Product FAQ

What consumables do I need to buy for using the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor?

Answer

The consumables needed for the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor are listed below:

- Pharma KingFisher Flex 96 Deep-Well Plates (Cat. No. A43075)
- Pharma KingFisher Flex 96 Standard Plates (Cat. No. A43076)
- Pharma KingFisher Flex Magnetic Head for 96 Deep-Well Plate (Cat. No. A31542)
- Pharma KingFisher Flex 96 Deep-Well Tip Combs (Cat. No. A43074)

Answer Id: E17124

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Product FAQ

Before doing a cell-based β-lactamase (BLA) assay, how long can I keep the cells in culture?

Answer

Shortly after receipt of the cells, we recommend making an early passage bank. The cells can be kept in culture for typically 2 months, with passaging twice per week.

Answer Id: E16537

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Product FAQ

Will total RNA FlashTag labeling affect the miRNA profile on the array?

Answer

No, specificity is defined by the array itself, using the recommended hybridization conditions. Single nucleotide discrimination is achieved when hybridizing the miRNA Arrays according to the FlashTag product insert.

Answer Id: E14054

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Product FAQ

Is the Versette Automated Liquid Handler compatible with any manufacturer's plates, including custom plates?

Answer

Yes, the Versette Automated Liquid Handler has an open platform for SBS* consumables used with automation. A Labware Library is available in the ControlMate software to select from a template list or add/modify the library with desired plates and labware customized by the user.

*SBS (Society of Biomolecular Screening) refers to the standard dimensions for plates in the market for any automated liquid handler.

Answer Id: E17141

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Product FAQ

How do I use the adaptor and compression pad for Applied Biosystems OpenArray off-line cycling on the Applied Biosystems GeneAmp PCR System 9700?

Answer

- Position the adaptor.
- Insert the two adaptor pins into the circular slots on the module.
- Be sure the adaptor sits flat.
- Position the Applied Biosystems TaqMan OpenArray plates.
- Orient each plate with the barcode facing up and along the edge of the block, away from the center of the adaptor.
- Fill up the right sample block first when placing the adaptor on the module (right sample block is the primary control block).
- Fill up the center slots before any edge slots.
- (Recommended) Insert unused TaqMan OpenArray plates into any empty slots.
- Place the compression pad over the TaqMan OpenArray plates (only needed for Applied Biosystems NT-OpenArray plates, not for Applied Biosystems QuantStudio 12K), with the Teflon coating (smooth side) facing upwards and away from the plates.
- Slide the heated cover over the compression pad, then pull down the handle.

For more detailed instructions, refer to the Dual Flat Block Applied Biosystems GeneAmp PCR System 9700 User Guide.

Answer Id: E10224

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Product FAQ

How do I determine lentiviral transduction efficiency?

Answer

ViraPower lentiviral expression kits contain a control lentiviral expression vector (the lacZ- expression vector or GFP- expression vector). When packaged into virions and transduced into a mammalian cell line, these vectors express either ß-galactosidase or GFP, respectively. If you use the lacZ control virus, you can use the ß-galactosidase staining kit (Cat. No. K1455-01) to determine transduction efficiency. If you use the GFP control virus, you can use a fluorescent microscope or perform FACS analysis.

Answer Id: E6384

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Product FAQ

Can the ELF 97 reagent be applied to live cells?

Answer

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Answer Id: E10803

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Product FAQ

What do you recommend for long-term storage of RNA?

Answer

For long-term storage, we recommend resuspending the RNA in stabilized formamide and storing at -70 degrees C. To remove the formamide, add 4 volumes ethanol and, if less than 20 µg RNA, also add NaCl to 0.2 M. Precipitate the RNA and use for downstream experiments.

Answer Id: E6543

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Product FAQ

Can the Pierce Magnetic Agarose Beads be used in an automated magnetic particle processor?

Answer

Yes. Protocols for using the beads on a KingFisher instrument are available on the product page.

Answer Id: E16149

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Product FAQ

For immunomagnetic separation (IMS) of environmental samples, what can be used as the starting material?

Answer

A 10 mL water concentrate with less than or equal to 0.5 mL packed pellet can be used in this procedure.

Answer Id: E4707

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Product FAQ

Can I use plates from other manufacturers with the Pharma KingFisher Flex 96 Deep-Well Magnetic Particle Processor?

Answer

No, we strongly recommend that you use Pharma KingFisher plates since they were specifically designed to be used with Pharma KingFisher Flex 96 Deep-Well Tip Combs to attain maximal performance. Plates from other manufacturers may not be compatible with the Pharma KingFisher Flex heating block. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.

Answer Id: E17125

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