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Regulatory requirements vary by product. Please see product-specific literature to determine product use.

What does the Silencer Select Human Genome siRNA Library V4 target? Product FAQ

Answer

siRNAs in the Silencer Select Human Genome siRNA Library V4 correspond to each of 21,584 genes, with 3 unique, nonoverlapping siRNAs provided per target, for a total of 64,752 siRNAs. The so called target genes correspond to greater than 98% of genes listed by NCBI that have at least one or more curated RefSeq coding transcripts. The siRNAs were designed to hit all RefSeq coding transcripts of that gene that were known at the time of design. The siRNAs targeting the druggable portion of this library are arranged by gene functional class to enable easy screening of important gene subsets.

Answer Id:: E10051

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How are the pre-plated, ready-to-ship Silencer Select siRNA Libraries plated? Product FAQ

Answer

The Silencer Select Human Genome siRNA Library and its subsets (Silencer Select Human Druggable Genome, Silencer Select Human Druggable Genome Extension Set, and Silencer Select Human Genome Extension Set) are plated in 384-well plates, each at 0.25 nmol siRNA per well. Each plate has the last 2 columns empty, and siRNAs to the same target are plated in different plates in the same well location. All other premade Invitrogen siRNA libraries are plated at 0.25 nmol siRNA per well in 96-well plates. Each plate has the last column empty, and siRNAs to the same target are plated in different plates in the same well location.

Answer Id:: E10055

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What do I do if I would like to order a fourth design of Silencer Select? Product FAQ

Answer

If you still would like to have 4 siRNAs in your library instead of 3, you have the option of adding 1 siRNA from our Silencer designs for the human library.

Answer Id:: E10070

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What is the absorbance/emission of the reaction product from the Vybrant Cell Metabolic Assay Kit? Product FAQ

Answer

The absorbance/emission is 563/587 nm.

Answer Id:: E10081

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What is the excitation/emission maxima of the digestion product from EnzChek Ultra Amylase Assay Kit? Product FAQ

Answer

The excitation/emission is 502/512nm.

Answer Id:: E10085

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Why is UDG important in the Platinum Quantitative PCR SuperMix-UDG mix? Product FAQ

Answer

UDG and dUTP are included to prevent the reamplification of carryover PCR products between reactions. dUTP ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs.

Answer Id:: E10089

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What caspase does the ApoTarget Caspase Colorimetric Protease Assay kit recognize? Product FAQ

Answer

The kit provides a simple and convenient means for quantitating the enzyme activity of caspases that recognize the amino acid sequences VDVAD (caspase-2), DEVD (caspase-3), VEID (caspase-6), IETD (caspase-8), and LEHD (caspase-9).

Answer Id:: E10093

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What optical filter do your recommend with the BacLight Green Bacterial Stain? Product FAQ

Answer

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

Answer Id:: E10097

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Is there a minimum length of template that can be used with the MEGAscript kit? Product FAQ

Answer

The MEGAscript Kit is designed to function best with templates that code for RNA transcripts of about 0.5 kb and longer. The kit can be used to produce shorter RNA, but modify the reaction as described in section “Optimizing yield of short transcripts” on page 24 of the manual.

Answer Id:: E10106

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Can you provide suggestions on how to introduce a fragment into the genome or important sequence by HDR (homology directed repair)? Product FAQ

Answer

Create a double-stranded DNA break using the GeneArt CRISPR Nuclease Vector (https://www.thermofisher.com/us/en/home/life-science/genome-editing/geneart-crispr/crispr-nuclease-vector.html), while simultaneously transfecting your plasmid-based donor repair template. Your donor repair template plasmid will contain the sequence you wish to introduce that is flanked by at least 500 bp (or more) of sequence, which results in efficient homologous recombination of your sequence.

Answer Id:: E10124

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What is the excitation and emission of OFP? What laser and emission filters should I use for detection? Product FAQ

Answer

OFP has a peak excitation of 548 nm, and emission of 560 nm. We recommend a 488 nm laser for efficient excitation. Standard 530/30, 647/26, and 603/48 emission filters are recommended for detection.

Answer Id:: E10153

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I see other publications using different PAM sequences such as NNNGATT, NNAGAA, or NAAAAC. Can I use that instead of NGG? Product FAQ

Answer

No, the PAM sequence is unique to the bacterial species that was used to create the Cas9. In the Invitrogen GeneArt kits, we derived Cas9 from Streptococcus pyogenes.

Answer Id:: E10157

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What are the advantages of the CRISPR mRNA system compared to your existing plasmid format? Product FAQ

Answer

The CRISPR mRNA system contains ready-to-transfect wild type Cas9 mRNA that circumvents the need for a cell type-specific promoter.

Answer Id:: E10161

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Can the GeneArt Genomic Cleavage Selection Kit be used to evaluate TALs and/or CRISPR constructs? Product FAQ

Answer

Yes, the GeneArt Genomic Cleavage Selection Kit can be used with either TALs or CRISPR constructs.

Answer Id:: E10174

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For how long are the reagents in the Invitrogen GeneArt CRISPR Nuclease Vector kit guaranteed? Product FAQ

Answer

All reagents are guaranteed to be stable for 6 months when properly stored. The vector, buffers, control oligo, plasmid, water, and sequencing primers should be stored at -20°C.

Answer Id:: E10151

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