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Regulatory requirements vary by product. Please see product-specific literature to determine product use.

Which of your protein gels can I run using the Mini Gel Tank? Product FAQ

Answer

Our New Bolt Bis-Tris Plus Mini gels (Cat. No. NWxxxxxBOX), as well as our Invitrogen Mini gels and NuPAGE Mini gels can be run using the Mini Gel Tank. Please note that our original Bolt Bis-Tris Plus Mini gels (Cat. No. BGxxxxxBOX, discontinued as of December 31, 2014) can only be run in the Bolt Mini Gel Tank (discontinued as of December 31, 2014, and will be offered until inventory is depleted).

Answer Id:: E10923

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Which of your protein gels can I run using the XCell4 SureLock Midi-Cell? Product FAQ

Answer

Our Midi gels can be run using the XCell4 SureLock Midi-Cell.

Answer Id:: E10937

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Why do you recommend using the PowerEase 300W Power Supply at limiting voltage for most applications? Product FAQ

Answer

The PowerEase 300W Power Supply is capable of operating at limiting voltage, limiting current, or limiting power. However, we recommend operating it at limiting voltage for most applications as it provides the following advantages:

- For most electrophoresis methods, resistance increases throughout the run. Under limiting voltage, current and power decrease throughout the run, providing an improving margin of safety over time.
- The same voltage setting can be used regardless of the number or thickness of gels being electrophoresed.
Note: With the current limiting setting, the voltage will increase as resistance increases to satisfy Ohm's law (V=IR). If no voltage limit is set and a local fault condition occurs, such as a poor connection, very high local resistance may cause the voltage to increase to the maximum capacity of the power supply. This may lead to local overheating and damage to the electrophoresis cell or create unsafe conditions. When operating under constant current conditions, we recommend setting a voltage limit on the power supply at or slightly above the maximum expected voltage. Power is a function of voltage and current P=IV. For a typical gel run, the current will decrease causing the voltage to increase when run at constant power. The power limiting function may be used when running sequencing gels to remove the ammonium persulfate from the wells and to heat the gel to an optimal temperature for DNA separations.

Answer Id:: E10953

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What are the components of the ZOOM IEF Fractionator? Product FAQ

Answer

The ZOOM IEF Fractionator includes the following components:

Chamber Assembly Tube with Anode Reservoir
Spill Trough with Cathode Reservoir Lid
Sample Chambers (7)
Sample Chamber O-ring Seals, red (10)
Sample Chamber Caps with O-rings (7)
Cathode End Sealer
Anode End Sealer
Cathode End Screw Cap
Spacers, black (8)
Spares Box 1
Sample Chamber O-ring Seals (8)
Sample Chamber Caps with O-rings (7)
Spares Box 2
Cathode Chamber Seals (2)
Spacers, black (8)

Answer Id:: E10973

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What kind of regular maintenance do you recommend for the ZOOM Dual Power Supply? Product FAQ

Answer

The ZOOM Dual Power supply requires no periodic maintenance program with the exception of an occasional dry wipe-down of the instrument.

Answer Id:: E10985

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My NativePAGE gel stops running halfway through the electrophoresis process. Can you please help me? Product FAQ

Answer

During NativePAGE runs, it is common for the current to drop below 1 mA. Most power supplies register this as a “No Load” error and automatically shut off, resulting in the stopping of the gel run. This can be bypassed in some power supplies by disabling or turning off the “Load Check” feature.

Answer Id:: E10989

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I have set up my gel to run in the XCell SureLock Mini-Cell and have switched on the power supply, but my gel is not running and there is no voltage or current reading on the power supply. What is wrong? Product FAQ

Answer

Here are some suggestions:

- Check the power supply unit.
- Double check that the tape on the bottom of the gel cassette has been removed.
- Make sure that the gel(s) are oriented so that the taller sides of the cassette (with the printing) are facing the outside of the electrophoresis unit.
- Make sure that the inner buffer chamber is filled sufficiently so that the wells are covered with buffer. If the wells are not covered, check for leaks and reseal.
- Double check to see if there are any loose electrodes or connections on the Mini Cell unit.

Answer Id:: E10993

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I am using your PowerEase 90W Power Supply. The operation stopped with an alarm and the screen displays: "Over Voltage" What is the problem? Product FAQ

Answer

Here are some suggestions: - Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.

Answer Id:: E11019

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I am getting a "Short Circuit" error on my PowerEase 90W Power Supply? What should I do? Product FAQ

Answer

Here are possible causes and solutions:

- Load exceeds 500 mA. The output maximum on the power supply is 500 mA, so make sure that it is not exceeded.
- Blown fuse in the power supply. Replace fuse following instructions on Page 17 of the manual.
- Incorrect input voltage. Check input voltage switch near power inlet.

Answer Id:: E11022

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How many degenerate sites are allowed in a 25-mer present at a total concentration of 1 micromolar (100 pmoles/100 mL)? Product FAQ

Answer

Primer mixtures with 256-fold and 32-fold degeneracies have been used [see Mack DH, Sninsky JJ (1988) Proc Natl Acad Sci USA 85:6977-6981 and Lee et al. (1988) Science 239:1288-1291.] We recommend that users synthesize pools of no more than 32- to 64-fold degenerate primers, making additional pools separately to account for all possible degeneracy. A matrix should be set up so that degeneracy is no more than 2-fold at each site, with all sites in the matrix run at the same time. Inosine may also be used for the degenerate positions in the primer. Performing touchdown PCR may help increase the specificity of degenerate primer PCR amplification.

Answer Id:: E1103

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Can I use SimplyBlue SafeStain to stain a PVDF for subsequent protein sequencing? Product FAQ

Answer

You can use SimplyBlue SafeStain to stain the PVDF membrane to detect your protein to be sequenced. After detection and prior to sequencing, rinse the membrane in 20-30% ethanol to remove the remaining stain.

Answer Id:: E11071

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What is the sensitivity of the PageBlue Protein Staining Solution? Product FAQ

Answer

It can detect proteins with a dynamic range of 5-500 ng, which is approximately 10 times more sensitive than traditional Coomassie R-250-based dyes.

Answer Id:: E11079

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What is the difference between Coomassie Blue G250 found in the Colloidal Blue Stain and SimplyBlue SafeStain, and Coomassie R250? Product FAQ

Answer

Coomassie G250 is more sensitive than Coomassie R250 whereas Coomassie R250 stains faster than Coomassie G250.

Answer Id:: E11099

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What is the expected level of sensitivity with the SilverQuest Silver Staining Kit? Product FAQ

Answer

The kit should be able to detect greater than or equal to 0.3 ng of protein.

Answer Id:: E11123

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What is the binding affinity for SYPRO Ruby Protein Gel Stain? Product FAQ

Answer

SYPRO Ruby Protein Gel Stain binds primarily to proteins through ionic charges of the dye, with basic side chains (lysine, arginine, histidine and to a lesser extent with tyrosine and tryptophan. The fixative solution and SYPRO Ruby stain solution both have an acidic pH, and SYPRO Ruby dye binding increases with protonated basic residues. SYPRO Ruby dye will also bind SDS bound to the proteins and in the gel matrix. The high 50% methanol concentration in the fixative solution is better at stripping out the SDS from the gel matrix, lowering the background staining and allowing for an optimal signal to noise.

Here is a reference on amino acid specificity of SYPRO Ruby Protein Gel Stain:
Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain. Steinberg TH, Chernokalskaya E, Berggren K, Lopez MF, Diwu Z, Haugland RP, Patton WF. Electrophoresis 2006, 21, 486-496.

Answer Id:: E11127

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