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Regulatory requirements vary by product. Please see product-specific literature to determine product use.

How can I analyze my CE data with hg19 primers after the transition to GRCh Build 38? Product FAQ

Answer

After transition to the GRCh Build 38 build, you should use software and analysis pipelines that still support hg19 to analyze your CE data. CE desktop software, such as Variant Reporter and SeqScape software, will continue to support hg19.

You can also perform analysis using the Variant Analysis (VA) or Next-Generation Confirmation (NGC) cloud apps, but you will need to create a reference using an hg19 Genbank file. If the hg19 Genbank file has annotations, VA or NGC will report out variants based on your provided annotations. In NGC, you will also need to provide the NGS variant file (vcf file) which is generated using hg19 as a reference.

Answer Id: E18500

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When using the Primer Designer Tool, what should the distance from a SNP of interest be relative to the 3' end of the primer? Product FAQ

Answer

When using the Primer Designer Tool, the suggested distance of the SNP of interest relative to the 3' end of the primer depends upon the BigDye sequencing kit being used, the use of M13 tails, and the polymer type. BigDye Direct and BigDye Terminator v1.1 typically have higher data quality very close to the sequencing primer end, whereas BigDye Terminator v3.1 together with POP‐7 polymer provides optimized data quality after the first 35 to 40 bases. Predesigned primers ordered with the M13 tails will allow the use of the universal M13 forward and M13 reverse primers for sequencing, the sequence immediately after the M13 primer will be the target primer sequence, and so the SNP can be close to the 3' end of the target primer sequence.

Answer Id: E18512

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Will I lose any data if I upgrade from the MicrobeBridge Software free trial version to the full version? Product FAQ

Answer

No data is lost with the MicrobeBridge Software installation of the full version on top of the free trial version.

Answer Id: E18535

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What is the viability of Gibco Rat Primary Cortical Astrocytes once thawed? Product FAQ

Answer

Upon thawing, the vial has more than 70% viability, which will give more than 1 million live cells.

Answer Id: E18547

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What are some of the key applications for astrocytes? Product FAQ

Answer

Astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.

Answer Id: E18553

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How do Lab Armor Beads perform under high or low temperatures? Product FAQ

Answer

Lab Armor Beads have been tested using various water bath models at a range of temperatures. In most baths, the further the set temperature is from ambient temperature, the greater the thermal gradient is farthest away from the heating element. Therefore, at an operating temperature of 37 degrees C to 42 degrees C, the thermal gradient in a water bath is typically +/- 1-3 degrees C, whereas at 55-65 degrees C it is closer to +/- 3-8 degrees C depending on the design of the bath and the amount of Lab Armor Beads used. To reduce the temperature gradient, use less Lab Armor Beads. For example, in a bath set at 65 degrees C, the temperature gradient can be reduced by 2 to 3 fold by using a 3-inch depth of Lab Armor Beads instead of 6-inch depth. For the most part, high temperature applications use microfuge tubes and other small vessels, so reducing the overall volume of Lab Armor Beads is very practical.

Answer Id: E18565

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How many cells are required to run one experiment with each TaqMan hPSC Scorecard kit/panel? Product FAQ

Answer

While we recommend that you use about 0.5 million cells per experiment, or the number of cells equivalent to 1 well of a 6-well dish, you can use as few as 100,000 cells when performing RNA purification. If you perform a lysis protocol using Cells-to-CT or CellsDirect One-Step qPCR kits, as few as 15,000 cells is sufficient. Please note, however, that reducing cell number can compromise the quality of the results.

Answer Id: E18583

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How long can I store StemPro Accutase Cell Dissociation Reagent at 2 to 8 degrees C? Product FAQ

Answer

Once thawed, we recommend using StemPro Accutase within 2 months. However, internal stability data suggests that the enzyme retains activity even after 1 year.

Answer Id: E18601

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How do you know that Gibco Rat Fetal Neural Stem Cells are indeed NSC? Product FAQ

Answer

We evaluated Gibco Rat Fetal Neural Stem Cells for two criteria:
- To express the NSC phenotype marker, Nestin and not express differentiated phenotype markers such as GFAP, Dcx, or GalC
- To demonstrate their differentiation potential toward all three downstream lineages such as neurons, astrocytes, and oligodendrocytes

Answer Id: E18607

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What are the estimated % neurons, astrocytes, and oligodendrocytes that we might get from using a spontaneous differentiation protocol vs. a directed differentiation protocol with Gibco Rat Fetal Neural Stem Cells? Product FAQ

Answer

Based on the expression of specific phenotypic markers, here are the expected percentages of the different cell populations:

Dcx marker (neuronal): 10% positive with spontaneous differentiation; 40% positive with directed differentiation

GFAP marker (astrocyte): 30% positive with spontaneous differentiation; 40% positive with directed differentiation

O4 marker (oligodendrocyte): 2% positive with spontaneous differentiation; 32% positive with directed differentiation

Answer Id: E18619

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What types of samples is the Oncomine BCR IGH SR Assay, RNA compatible with? Product FAQ

Answer

This assay is compatible with isolated B cells, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh frozen tissue samples, including FFPE and bone marrow samples.

Answer Id: E18637

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How much of each sample is needed for the Ion AmpliSeq Microbiome Health Research Kit? Product FAQ

Answer

The minimum sample input is 1 ng/pool for the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool and 10 ng/pool for the Ion AmpliSeq Microbiome Health Research - Target Species Pool.

Answer Id: E18237

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How long can I use Diploid Growth Serum-Reduced Medium (SRM) supplemented with Diploid Growth Supplement? Product FAQ

Answer

Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.

Answer Id: E18248

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For the Dual LED Blue/White Light Transilluminator, can I purchase the white/blue plates and imaging hood as standalone items? Product FAQ

Answer

The white/blue plates and imaging hood are not sold separately.

Answer Id: E18254

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How big do DNA fragment homology arms need to be when using Gibson Assembly kits? Product FAQ

Answer

For fragments that are 8 kb or lesser in length, an overlap of 20-40 bp is recommended. For fragments greater than 8 kb in length, an overlap of 40-100 bp is recommended.

Answer Id: E18260

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