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Dynamics of claudins expression in colitis and colitis-associated cancer in rat. Citations & References

  • Authors: Arimura Y, Nagaishi K, Hosokawa M,
  • Journal: Methods Mol Biol
  • PubMed ID: 21717373

Barnstead Cartridge and Filter Systems Quick Select Guide Product Literature

AP-1 (Fra-1/c-Jun)-mediated induction of expression of matrix metalloproteinase-2 is required for 15S-hydroxyeicosatetraenoic acid-induced angiogenesis. Citations & References

  • Authors: Singh NK, Quyen DV, Kundumani-Sridharan V, Brooks PC, Rao GN
  • Journal: J Biol Chem
  • PubMed ID: 20353950
Catalog #

15-Lipoxygenase-2 is expressed in macrophages in human carotid plaques and regulated by hypoxia-inducible factor-1α Citations & References

  • Authors: Hulten, LM; Olson, FJ; Aaberg, H; Carlsson, J; Karlstroem, L; Boren, J; Fagerberg, B; Wiklund, O
  • Journal: European Journal of Clinical Investigation
Catalog #
  • 4339386
  • 4413750
  • N8050400(Discontinued)
  • N8050200(Discontinued)
  • N8050002(Discontinued)
  • ZGEXSC9700384W
  • ZGEXSC9700384W3Y
  • N8050002R
  • 4441166

Opposing Effects of 15-Lipoxygenase-1 and -2 Metabolites on MAPK Signaling in Prostate. ALTERATION IN PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR gamma. Citations & References

  • Authors: Hsi Linda C.; Wilson Leigh C.; Eling Thomas E.;
  • Journal: Others
  • PubMed ID: 12189136
Catalog #

15(S)-hydroxyeicosatetraenoic acid-induced angiogenesis requires Src-mediated Egr-1-dependent rapid induction of FGF-2 expression. Citations & References

  • Authors: Kundumani-Sridharan V, Niu J, Wang D, Van Quyen D, Zhang Q, Singh NK, Subramani J, Karri S, Rao GN
  • Journal: Blood
  • PubMed ID: 20053757
Catalog #
  • 4379633
  • 4359284
  • 4379635
  • 4359286
  • ZGEXSC7300(Discontinued)
  • ZGEXSC73003Y(Discontinued)
  • 4350814(Discontinued)
  • 111
  • 4330087R
  • 4351103
  • 4367246
  • 4351101
  • 4351103R
  • 4363914
  • 4363915
  • 4351101R

Barnstead Water Purification Cartridge and Filter Systems Product Literature

How do I remove trace contaminants from the Slide-A-Lyzer MINI Dialysis Unit? Product FAQ

Answer

To remove glycerol, dialyze for 15 minutes against 1 liter of deionized water. Glycerol content is less than 3% in the 3K unit, approximately 15% in the 7K unit and 23% in the 10K unit. To remove metals, dialyze for 15 minutes against 1 liter of 1 mM EDTA. Metals present in the 3K, 7K and 10K unit include 2 ppb iron, 5 ppb magnesium, 1.5 ppb nickel, 0.2 ppb zinc, 0.2 ppb copper, 0.5 ppb chromium and 0.3 ppb cadmium. To remove sulfur, dialyze for 15 minutes against 1 liter of deionized water. Sulfur content should be minimal, less than 0.1%. Thermo Scientific quality control checks for sulfur on the membrane prior to the unit's assembly.

Answer Id:: E8295

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1-Oleoyl-2-acetyl-glycerol and phorbol diester stimulate Ca2+ influx through Ca2+ channels in neuroblastoma x glioma hybrid NG108-15 cells. Citations & References

  • Authors: Osugi T, Imaizumi T, Mizushima A, Uchida S, Yoshida H
  • Journal: Eur J Pharmacol
  • PubMed ID: 2428631
Catalog #
  • O1538
  • Q1288
  • P3454
  • Q1289

How can I optimize my ligation reaction? Product FAQ

Answer

Please consider the following suggestions:
1– Try different molar ratios of insert to vector. Having an excess of insert is usually what will work, try 1:1 to 15:1 insert:vector.
2– Try increasing the time of the ligation at 37 degrees C.
3– Try performing the ligation at 16 degrees C overnight (you can set it up on your PCR machine).

Answer Id:: E6785

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How long does the GeneChip eukaryotic expression assay take from sample preparation to obtaining the array image? Product FAQ

Answer

There is some variation in processing time depending on sample type and array type, however, in general, starting from total or poly-A mRNA, the entire GeneChip eukaryotic expression assay take approximately 2 1/2 days (assuming 8 hrs in a day). Target labeling takes approximately 2 days, followed by overnight hybridization to a GeneChip expression array. Washing and staining takes approximately 1 hr and 15 mins, and scanning varies from 10-35 mins.

Answer Id:: E13585

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