Learn More Anza Restriction Enzymes
Introducing the Invitrogen Anza Restriction Enzyme Cloning System, a complete, one-buffer system of restriction enzymes and DNA-modifying enzymes–for beautifully simple cloning. Request a free Anza poster Download brochure One buffer for all restriction and DNA modifying enzymes One digestion...
Download PDF version › Invitrogen™ Lipofectamine™ 3000 Transfection Reagent is a highly efficient, cost-effective tool for lentiviral production (Figure 1). This versatile reagent enables high viral titers even with genes that are large or difficult to package.
Transgenic mice are widely used as a model organism in basic research to study the role of genes in development, physiology, and human diseases. PCR is a common genotyping method to detect the presence or absence of the gene(s) of interest in transgenic mice.
To prepare an acceptable, final 1X solution, perform the following procedure under aseptic conditions. Use DistilledWater (Cat. no. 15230) and 7.5% Sodium Bicarbonate Solution (Cat. no. 25080) for use in this protocol.
Our array of custom antibody services includes a customizable option for guinea pig immunization, serum collection, and titer analysis for guinea pig antibody production, plus several extension possibilities.
Learn More T Cell Activation via Anti-CD3 and Anti-CD28
T cell activation via the αβ-T cell receptor (TCR complex) is required for in vitro expansion. Treatment of T cells with monoclonal anti-CD3 antibodies and anti-CD28 antibodies provide a co-stimulatory signal that engages the TCR which can be used for antigen-induced activation.
Learn More Recommended Sample Preparation Procedures
Whole Blood and Buffy Coat Whole blood or buffy coat can be used as starting samples. In some cases, it is necessary to wash the sample to remove interfering soluble factors. Tissue Digests Follow standard tissue preparations using enzymes and mechanical disruption to get a single cell suspension.
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Learn More iNKT Cell Identification Protocol
Invariant natural killer T (iNKT) cells secrete a wide variety of cytokines and rapidly respond to lipid antigenic stimulation [1–2] . They are a CD1d-restricted population of T cells which can be uniquely identified based on the expression of CD1d T cell receptors (TCRs) as well as typical T...
Learn More Pegasus ESD Test System
The Thermo Scientific Pegasus ESD Test System is a low parasitic 2-pin tester used to characterize protection structures at wafer, die, or package level. The Pegasus can also be used as part of your device qualification routine, providing supplemental data to the results gathered when using an...
Ensure consistent and accurate conductivity, resistivity, total dissolved solids (TDS) and salinity measurements with Orion conductivity probes. Thermo Scientific Orion DuraProbe 4 Cell Lab and Field Conductivity Probes provide the highest accuracy for demanding laboratory or field applications with...
Learn More Western Blot Protocols and Recipes
A western blot experiment, or western blotting, is a routine technique for protein analysis. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture.
The FIX & PERM Cell Fixation and Cell Permeabilization Kit achieves mild fixation and permeabilization of cells that leaves their morphological scatter characteristics intact, enabling researchers to accurately identify previously undetectable intracellular markers, such as cytoplasmic or nuclear...
Learn More Stability of miRNA at Sub-Zero Temperatures
Storage of purified RNA at sub-zero temperatures (usually –20ºC or –80ºC) is a time-tested method for ensuring stability of the entire RNA population without bias. Recently, some researchers have questioned whether chemical instability or RNA adherence to tube surfaces skews representational levels...
Learn More GeneChip Array Annotation Files
Annotation files are for use by any interested party to understand what biological entities are represented on GeneChip™ brand arrays. Annotation information is stored in a single file for each array type. This information is updated quarterly.