Abstract Procedure Overview Step 1: Bisulfite Conversion of DNA Step 2: Primer Design Step 3: MS-HRM Optimization Step 4: MS-HRM Standard Curve and Data Analysis Step 5: Sequencing the HRM Product Table References, Download the PDF version of this web page: DNA Methylation Analysis Using...
A well-established technique for identifying chemicals, Raman spectroscopy has been used in forensic laboratory analysis#3;for decades. It is widely accepted by the scientific community and has a proven track record in the United States court system.
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Learn More NMR Spectrum of Propranolol Hydrochloride
(±)-Propranolol hydrochloride (C 16 H 21 NO 2 · HCl), a nonselective beta-blocker, is representative of a class of drugs that target the beta receptor. It is prescribed medication for treatment of hypertension, severe headaches, and cardiac related dysrhythmia and ischemia.
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Introduction What is gene expression? Real-Time PCR concepts Gene expression using real-time PCR Selecting the detection chemistry Selecting or designing assays Selecting the quantitation method Analyzing data Biography Ordering Information, Gene expression or messenger RNA (mRNA) analysis is the...
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Activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell, plays an important role in apoptosis. Thermo Fisher Scientific offers reagents and kits for imaging caspase activity in real-time or as an end-point assay.
Learn More No-Wash Imaging of Apoptosis: CellEvent™ Caspase 3/7 Green Detection Reagent For Apoptosis
An Easy and Flexible Method for Measuring Activated Caspase 3/7 The CellEvent™ Caspase-3/7 Green detection reagent consists of the DEVD peptide sequence conjugated to a nucleic acid–binding dye. The DEVD peptide inhibits the ability of the dye to bind to DNA and thus the substrate is intrinsically...
Learn More Hematopoiesis from Pluripotent Stem Cells
Hematopoietic stem cells (HSCs) have the property of self-renewal and,, through cell division and differentiation, form populations of progenitor cells which are committed to the main marrow cell lines: erythroid, granulocytic and monocytic, megakaryocytic, and lymphocytic.
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Invariant natural killer T (iNKT) cells secrete a wide variety of cytokines and rapidly respond to lipid antigenic stimulation [1–2] . They are a CD1d-restricted population of T cells which can be uniquely identified based on the expression of CD1d T cell receptors (TCRs) as well as typical T...
Avoid delays in moving your innovations to market, Every day has the potential to be critical when you work at a biotechnology company. Today may be the day you get funding on a new project, or it may be the day that you get data that propels you down a new path of development.
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The EVOS™ FLoid™ Cell Imaging Station is an affordable, user-friendly imaging solution for the quick detection and verification of fluorescently labeled samples. It allows you to capture transmitted light and three-color fluorescent images of your cells and samples right at your benchtop.
Find technical resources for the Invitrogen Countess II, including manuals, application notes, software download information, and ordering information for consumables and accessories for your instrument.
Learn More Coomassie Blue Gel and Membrane Stains
The most common method for in-gel protein detection is staining with Coomassie blue dye. Coomassie dye staining is especially convenient because it involves a single, ready-to-use reagent and does not permanently chemically modify the target proteins.