Learn More IHC Buffer Recipes
Buffer 1: 10 mM PBS, pH 7.4 Buffer 2: 0.1 M NaHCO3, pH 9.6 Buffer 3: 10 m M PBS, pH 7.4 with TWEEN 20 Buffer 4: 0.1 M Citrate, pH 4.2 with 0.03% H2O2 Buffer 5: 50 mM Tris-buffered Saline, pH 7.5 Buffer 6: 0.1 M Amino-Methyl- Propanediol, pH 10.3 Buffer 7: 0.1 M Citrate-Phosphate, pH 5.0, with 0.
Learn More Carboys
Nalgene carboys are time-tested workhorses in the lab, known for their leakproof guarantee and unmatched rugged construction. Nalgene carboys are made from high-quality plastic resin materials, and meet rigorous standards.
Learn More Quality Control Webinar: Simplify your IQCP
On January 1, 2016, EQC will cease to be legal. All labs running EQC must either revert back to the US CLIA minimums or develop and switch to an Individual Quality Control Plan (IQCP). With less than 9 months to go, IQCP is still clouded in mystery.
Aspirate the medium from culture dish and wash with 4mL of DPBS (w/o calcium and magnesium, Cat. No 14190). Aspirate DPBS and add 2 mL of Accutase® to culture dish. Incubate for 2 to 5 minutes at 37 °C until individual single cells start to round up.
Learn More FAIMS Pro Interface Support Getting Started
Optimize your experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your analyses needs. View the relevant questions below:, Browse our FAQ database for more information ›, Having problems with your experiment?
Learn More microRNA Expression Analysis Research
Effective method of miRNA isolation from samples RT-PCR reagents optimized for miRNA detection Assays specific to the miRNAs of interest Real-time analytical instruments and reagents validated for miRNA detection protocols, Lewis BP, Burge CB, Bartel DP (2005) Conserved seed pairing, often flanked...
Learn More Varioskan LUX Multimode Microplate Reader
For Research Use Only. Not for use in diagnostic procedures., Our newest multimode microplate reader combines simplicity with top-of-the-line performance. Designed for researchers with a wide variety of needs, the Thermo Scientific Varioskan LUX Multimode Microplate Reader offers features that help...
Learn More Variation of microRNA Profiles
Sylvestre Y, De Guire V, Querido E, Mukhopadhyay UK, Bourdeau V, Major F, Ferbeyre G, Chartrand P (2007) An E2F/miR-20a autoregulatory feedback loop. J Biol Chem 282(4):2135−43. Pogribny IP, Tryndyak VP, Boyko A, Rodriguez-Juarez R, Beland FA, Kovalchuk O (2007) Induction of microRNAome deregulation...
NovaBright reporter gene assays offer chemiluminescent measurement of reporter gene activity that is more sensitive than colorimetric or fluorescent detection. NovaBright kits are available for the most common reporter genes, and are optimized to yield consistent results with high sensitivity over a...
Learn More Guide To Setting Up Hypoxic Conditions On the EVOS FL Auto Imaging System With Onstage Incubator
Download PDF version Cellular responses to reduced oxygen—hypoxic conditions—have been linked to a wide range of human pathologies, including tumor development, atherosclerosis, inflammation, and abnormal angiogenesis.
Learn More NMR Spectrum of Quercetin
Quercetin (C 15 H 10 O 7 ), 3,3',4',5-7-pentahydroxyflavone, is a flavonol, and acts as a pigment in many fruits and vegetables, flowers, and leaves; the color of red onions and apples is caused by quercetin. The 82 MHz spectrum of a 9.8% (w/w; 0.
Learn More Helios 5 Hydra DualBeam
The Thermo Scientific Helios 5 Hydra DualBeam (plasma focused ion beam scanning electron microscope, PFIB-SEM) can deliver four different ion species as the primary beam, allowing you to choose the ions that provide the best results for your samples and use cases, such as scanning transmission...
Learn More Biogeochemistry
Researchers can obtain unique insights into biological and biogeochemical processes from the isotopic signatures of a wide variety of complex organic compounds. Isotope signatures vary as chemical and physical processes lead to changes in the natural isotope composition of these compounds.
Cellular differentiation studies require sensitive tools to detect subtle phenotypic changes in cellular signaling pathways and morphology. In the present study, we used luciferase assays and high-content immunofluorescence cellular imaging to monitor phenotypic changes in neuronal precursor cell...
Learn More Polymethylpentene (PMP) Labware
Polymethylpentene is classified as a polyolefin and is a high-molecular weight hydrocarbon. Polymethylpentene is similar to polypropylene, but has an isobutyl group instead of a methyl group attached to each monomer group of the chain. Its chemical resistance is close to that of PP.