Citations & References

Effects of 2-mercaptoethanol on survival and differentiation of fetal mouse brain neurons cultured in vitro.

  • Authors: Ishii K, Katayama M, Hori K, Yodoi J, Nakanishi T
  • Journal: Neurosci Lett 1993 (163):159-162
  • PubMed ID: 8309623

Citations & References

Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-Phthaldialdehyde/ 2-mercaptoethanol reagent

  • Authors: Lee, K., et al.
  • Journal: J Biol Chem July (1979) 25:6248-6251
Catalog #

Citations & References

Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-phthaldialdehyde/2-mercaptoethanol reagent.

  • Authors: Lee KS, Drescher DG
  • Journal: J Biol Chem (1979) 254:6248-6251
  • PubMed ID: 109447
Catalog #
  • P2331MP(Discontinued)

Citations & References

2-Mercaptoethanol is a survival factor for olfactory, cortical and hippocampal neurons in short-term dissociated cell culture.

  • Authors: Grill RJ, Pixley SK
  • Journal: Brain Res 1993 (613):168-172
  • PubMed ID: 8348301

Citations & References

Simple and sensitive determination of plasma N tau-methylhistidine by high-performance liquid chromatography using pre-column derivative formation with o-phthalaldehyde-2-mercaptoethanol.

  • Authors: Nagasawa T, Sakai T, Onodera R
  • Journal: J Chromatogr (1991) 566:223-227
  • PubMed ID: 1885715
Catalog #
  • P2331MP(Discontinued)

Citations & References

Micellar Enhanced Fluorimetric Determination of 1-N,N-Dimethylaminonaphthalene-5-sulfonyl Chloride and o-Phthalaldehyde-2-Mercaptoethanol Derivatives of Amino Acids.

  • Authors: Singh HN, Hinze WL
  • Journal: Analyst (1982) 107:1073-1073
Catalog #
  • D21(Discontinued)
  • P2331MP(Discontinued)

Citations & References

The Oxidative Addition Reaction between Compounds of Resorufin (7-Hydroxy-3H-phenoxazin-3-one) and 2-Mercaptoethanol

  • Authors: Kitson TM
  • Journal: Bioorg Chem (1998) 26:63-73
Catalog #
  • R352(Discontinued)
  • R6564(Discontinued)

Product FAQ

I've purchased Gibco 2-Mercaptoethanol (Cat. No. 21985023). Should I add it to my cell culture medium right before every use or can I add it to the bulk medium in advance?

Answer

The 2-Mercaptoethanol must be added to the medium immediately before use because activity rapidly declines in diluted form. You can supplement enough medium needed for that day.

Answer Id: E17388

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Product FAQ

What additional products do I need to make a complete StemPRO hESC SFM?

Answer

In addition to the StemPRO hESC SFM kit, you will also need the following to make a complete medium: full length recombinant human FGF (catalog # PHG0261) and 2-Mercaptoethanol (catalog # 21985-023).

Answer Id: E5297

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Product FAQ

I am using the PureLink RNA Micro Kit, and my RNA yield is very low. Can you offer some tips?

Answer

This could be due to incomplete lysis and homogenization. Here are some recommendations:

- Ensure that 10 µL of 2-mercaptoethanol was added per 1 mL of Lysis Buffer.
- Perform all steps at room temperature unless directed otherwise.
- Decrease the amount of starting material used, or increase volume of Lysis Buffer.
- Use the proper homogenization methods according to recommendations in the sample-specific protocols.
- Cut tissue samples into smaller pieces and ensure the tissue is completely immersed in the Lysis Buffer to achieve optimal lysis.

Answer Id: E9743

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Product FAQ

How is DSP cleaved?

Answer

The disulfIde bond in DSP's 12Å spacer arm can be cleaved by at least three different methods. The first is by incubating the crosslinker in 10-50 mM DTT for 30 minutes at 37 degrees C. Secondly, the disulfIde can be reduced by incubation in 5% 2-Mercaptoethanol (2-ME) in SDS-PAGE sample buffer at 100 degrees C for 5 minutes. Finally, the “-S-S-” bond can be cleaved by incubation in 100 mM sodium borohydride at room temperature for 30-60 minutes. Other reducing agents such as TCEP-HCl or 2-Mercaptoethylamine (2-MEA) should also work, however these applications have not been referenced.

Answer Id: E13317

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Product FAQ

I’m getting low RNA yield when using the PureLink RNA Mini Kit. What could be the cause of this and what do you suggest I try?

Answer

Low RNA yield can occur due to the following:

- Incomplete lysis and homogenization: ensure that 10 µL of 2-mercaptoethanol was added per milliliter of lysis buffer, perform all steps at room temperature, decrease the amount of starting material used, use proper homogenization methods, and/or cut tissue samples into smaller pieces to ensure complete tissue immersion in the lysis buffer.
- Poor quality starting material: use fresh samples and process immediately after collection.
- Ethanol may not have been added to Wash Buffer II.
- Incorrect elution conditions may have been used: Add RNase-free water and incubate for 1 minute before centrifugation, following the recommendations for elution in the manual. You can also perform a second elution step to recover more RNA.

Answer Id: E7875

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Product FAQ

How do I prepare the StemPRO hESC SFM complete medium?

Answer

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Answer Id: E5304

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Product FAQ

Are samples containing EDTA or reducing agents compatible with the Thermo Scientific Phosphoprotein Enrichment kit?

Answer

No. Chelating agents such as EDTA and EGTA will cause metal to leach from the column and hence, reduce binding. Reducing agents such as DTT, 2-mercaptoethanol and TCEP also reduce binding to the column. Remove incompatible substances from the sample by desalting before applying to the column.

Answer Id: E8238

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