Product Literature

CD 293 Medium

Product FAQ

Can I add L-photo-leucine to Freestyle 293 Expression Medium in order to obtain a secreted protein incorporated with photo-leucine?

Answer

To use the L-photo-leucine, you will need to use a medium that is free of L-leucine. The 293 medium has all essential amino acids so they will compete with the photo amino acids for incorporation into your proteins. Our product DMEM-LM (Cat. No. 30030) is deficient in L-leucine and L-methionine.

Answer Id: E15744

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Product FAQ

What is the difference between FreeStyle 293 Expression Medium and FreeStyle F17 Expression Medium?

Answer

FreeStyle F17 Expression Medium was designed from a different base formulation than FreeStyle 293 Expression Medium. FreeStyle F17 medium shows equal or better performance than the FreeStye 293 medium for certain cell lines and proteins. It is capable of supporting the growth of 293 and CHO cells and offers a wider variety and selection of transient cell culture media options.

Answer Id: E17365

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Product FAQ

Why do FreeStyle 293-F cells require 8% CO2 for culturing?

Answer

The CO2 requirement is dependent on the media. Our serum-free 293 media require 8% CO2 to best buffer the media.

Answer Id: E17452

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Product FAQ

How are FreeStyle 293-F cells different from regular HEK293 cells?

Answer

FreeStyle 293-F cells are derived from HEK293 cells; they were just cloned extensively for growth, viability, and protein production in the new serum-free FreeStyle 293 medium. Thermo Fisher Scientific coined the name “FreeStyle” to signifiy the freedom of switching the cells to the defined serum-free FreeStyle media system.

The parental HEK293 cells, if adapted to the same media as FreeStyle 293F cells, should have the same transfection efficiency. However the final protein production levels may be very different as the FreeStyle 293F cells have been cloned and selected for maximal protein production.

Answer Id: E17397

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Product Literature

Human Embryonic Kidney 293

Manual / Product Insert

Growth and Maintenance of the GripTite 293 MSR Cell Line

Version: 3 January 2012
Catalog #

Manual / Product Insert

User Guide: FreeStyle 293-F Cells

Version: MAN0007924 Rev. 2.0
Catalog #

Manual / Product Insert

User Guide: 293 Cells, SFM Adapted

Version: MAN0007367 Rev A.0 (10 September 2015)

Product Literature

Human Embryonic Kidney (293) C

Product Literature

FreeStyle 293 Expression Syste

Manual / Product Insert

Tango™ OPRM1-bla U2OS Cell Line Validation Packet

Version: version 1.1

Manual / Product Insert

Jump-In TI Gateway Targeted Integration System

Version: Version B, 5 Feb 2009
Catalog #
  • A10897
  • A10895(Discontinued)
  • A10896(Discontinued)

Product Literature

Specialty and Serum-Free Media