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User Guide: Zeba Spin Desalting Columns and Plates 40K MWCO Manual / Product Insert

  • Version: Jan. 2015
Catalog #
  • 87768(Discontinued)
  • 87769(Discontinued)
  • 87766(Discontinued)
  • 87767(Discontinued)
  • 87775(Discontinued)
  • 87764(Discontinued)
  • 87765(Discontinued)
  • 87773(Discontinued)
  • 87774(Discontinued)
  • 87771(Discontinued)
  • 87772(Discontinued)
  • 87770(Discontinued)

Can you provide the shelf life for Zeba Spin Desalting columns? Product FAQ

Answer

Zeba Spin Desalting columns are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E18763

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Can Zeba Columns be used for glycerol and detergent removal? Product FAQ

Answer

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although dialysis is a better choice for removing these components, Zeba Columns can be used by modifying the desalting protocol. Modifications include diluting the samples, processing smaller samples, and adding the sample slowly to the column allowing it to enter the gel and equilibrate into the pores before centrifugation.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12869

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Is my sample likely to become diluted following desalting with Zeba columns? Product FAQ

Answer

Centrifuged-based desalting results in minimal dilution. A slight dilution of the protein solution results if the optional stacker buffer is added that ensures maximum protein recovery.

When used properly, the volume recovered from the column equals the volume of sample (and buffer) added to the column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12868

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I used a Zeba desalting column, but it is not completely getting rid of glycerol and detergent from my sample. What should I do? Product FAQ

Answer

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although dialysis is a better choice for removing these components, Zeba Columns can be used by modifying the desalting protocol. Modifications include diluting the samples, processing smaller samples, and adding the sample slowly to the column allowing it to enter the gel and equilibrate into the pores before centrifugation.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12877

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What is desalting? Product FAQ

Answer

Desalting is the process where porous particles or beads are used to separate molecules of different sizes (known as gel filtration or size exclusion). Small molecules enter into the pores in the resin, resulting in a longer path length through the desalting column when compared to large molecules which pass in the space between the beads This increased path length for molecules below the MWCO allows the separation of small and large molecules.

Our desalting resins are rated by what MWCO molecules can pass through. They also have a lower MWCO (1K or 2K) that can be effectively separated from larger molecules. Molecules in between these values cannot be effectively separated from larger or smaller molecules, given our protocols.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12862

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How do your Zeba desalting products work? Product FAQ

Answer

A centrifuge is used to first remove the resin‘s void volume of liquid, followed by sample addition and centrifugation. After centrifugation, the macromolecules in the sample have moved through the column in approximately the same initial volume, but the small molecules have been forced into the pores of the resin and replaced by the buffer that was used to pre-equilibrate the gel-filtration matrix. Spin formats eliminate the need to wait for samples to emerge by gravity flow and require no chromatography system, allowing for multiple-sample processing simultaneously.

Note: The addition of larger volumes of buffer or longer centrifugation times than listed in the protocol will result in smaller molecules eventually emerging from the desalting column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12863

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Can I remove DyLight, Alexa Fluor or other dyes using the desalting columns? Product FAQ

Answer

No, excess amounts of DyLight dyes (and any other planar molecule with multiple rings in their structure) “act” larger than their stated molecular weights in desalting columns. We do not recommend desalting, but refer researchers to our Pierce Dye Removal Columns (Cat. No. 22858) for this purpose.

Answer Id: E12866

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Are Zeba columns compatible with salts/organics? Product FAQ

Answer

Yes, Zeba columns are compatible with most salts. The resin is stable to some organics. As organics may affect performance, we suggest using less than 10% organics.

Answer Id: E12864

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After desalting with your Zeba desalting columns, I still have leftover salts in my sample. What should I do to get rid of all the salt? Product FAQ

Answer

You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.

Answer Id: E12876

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Can these columns be regenerated and stored after use? Product FAQ

Answer

The desalting columns are designed to be used once and discarded after use.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Answer Id: E12867

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Can I use Zeba columns for nucleic acids? Product FAQ

Answer

Yes, the 7K Zeba columns have good recovery of dsDNA ladder down to 10 bp.

Answer Id: E12865

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Lot # specs Certificate

Catalog # 87771(Discontinued)

RF8259 SDS

Catalog #
  • 87768(Discontinued)
  • 87769(Discontinued)
  • 87766(Discontinued)
  • 87767(Discontinued)
  • 87775(Discontinued)
  • 87764(Discontinued)
  • 87765(Discontinued)
  • 87773(Discontinued)
  • 87774(Discontinued)
  • 87771(Discontinued)
  • 87772(Discontinued)
  • 87770(Discontinued)
  • RF8259
  • 8259
  • 87771LCS