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AKT1S1 Synthetic Peptide (Invitrogen™)

Human PRAS40 Synthetic Peptide for BLOCK, Ctrl

Phospho-PRAS40 (Thr246) Polyclonal Antibody (Invitrogen™)

Phospho-PRAS40 (Thr246) Polyclonal Antibody for Western Blot, IHC

Phospho-PRAS40 (Thr246) Antibody (8HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

Phospho-PRAS40 (Thr246) Oligoclonal Antibody for Western Blot, IF, ICC

PRAS40 (Phospho) [pT246] Human ELISA Kit (Invitrogen™)

PRAS40 (Phospho) [pT246] Human ELISA Kit for ELISA

PRAS40 Monoclonal Antibody (73P21) (Invitrogen™)

PRAS40 Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

Phospho-PRAS40 (Thr246) Antibody (8H13L13), ABfinity™ Rabbit Monoclonal (Invitrogen™)

Phospho-PRAS40 (Thr246) Monoclonal Antibody for Western Blot, IF, ICC

PRAS40 Antibody (4HCLC), ABfinity™ Rabbit Oligoclonal (Invitrogen™)

PRAS40 Oligoclonal Antibody for Western Blot

LanthaScreen™ Tb-anti-PRAS40 [pSer183] Antibody Kit

The LanthaScreen® Tb-anti-PRAS40[pSer183] antibody has been designed and validated for use in the LanthaScreen® PRAS40 HEK 293E Cellular Assay, to facilitate in-house assay configuration and screening. These antibodies may be accessed in small scale and screening quantities.

PRAS40 Antibody (4H14L8), ABfinity™ Rabbit Monoclonal (Invitrogen™)

PRAS40 Monoclonal Antibody for Western Blot, IF, ICC

LanthaScreen™ PRAS40 293E Cell Line

The PI3K/AKT pathway is activated by various stimuli (including insulin and IGF-1) and mediates signals for cell growth, cell survival, translation, and inhibition of apoptosis. PRAS40 (Proline-rich AKT substrate, 40 kDa) is a cytosolic protein that is phosphorylated at position Thr246 by AKT directly upon stimulation of the pathway. PRAS40 has been shown to bind to the mTORC1 complex and inhibit downstream signaling (i.e., phosphorylation of 4E-BP1 or p70S6K). LanthaScreen® PRAS40 HEK293E is a human cell line which constitutively expresses GFP-PRAS40 fusion proteins. The kinase substrate
was introduced using lentiviral transduction followed by selection with blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as a sorting marker. The PI3K/AKT pathway is known to be active in the highly insulin-responsive HEK293E cell background. Using this cell line, a homogenous ("addition only") immunoassay has been developed in a 384-well format. The phosphorylation of PRAS40 at Thr246 is detected in cell lysates using a terbium-labeled phosphospecific antibody in a time-resolved FRET (TR-FRET) readout. The performance of this assay has been tested using numerous experimental variables, including cell plating density, stimulation time, DMSO tolerance, and antibody incubation time. Under optimized conditions, the assay has been further validated and shows correct EC50 and IC50 values, with insulin as the primary agonist and PI-103 as the known inhibitor. Overall, the assay displays excellent statistical data (Z'-factor >0.6), high signal-to-background (response ratio), and is a robust cell-based readout of AKT signaling.

PRAS40 Polyclonal Antibody (Invitrogen™)

PRAS40 Polyclonal Antibody for Western Blot, IF, ICC, IHC

PRAS40 Polyclonal Antibody (Invitrogen™)

PRAS40 Polyclonal Antibody for Western Blot, IHC (P)

PRAS40 Polyclonal Antibody (Invitrogen™)

PRAS40 Polyclonal Antibody for Western Blot, IHC (P)

Phospho-PRAS40 (Thr246) Polyclonal Antibody (Invitrogen™)

Phospho-PRAS40 (Thr246) Polyclonal Antibody for Western Blot

PRAS40 Polyclonal Antibody (Invitrogen™)

PRAS40 Polyclonal Antibody for Western Blot