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FL-AR N-TERM Peptide

LanthaScreen® fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen® TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen® panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen® TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:

LanthaScreen® fluorescent nuclear receptor coregulator peptides are supplied as 100 µM solutions in peptide-appropriate buffers. Store peptides at -20°C.

GeneBLAzer™ AR-UAS-bla Griptite™ Cells

AR-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the rat Androgen receptor (AR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer®UAS-bla GripTite™ 293 cell line. GeneBLAzer®UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-AR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. AR-UAS-bla GripTite™ 293 cells are functionally validated for Z' and EC50 concentrations of R1881. Cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Amphiregulin Monoclonal Antibody (AREG559), PE-Cyanine7, eBioscience™ (Invitrogen™)

Amphiregulin Monoclonal Antibody for Flow

Amphiregulin Monoclonal Antibody (AREG559), APC, eBioscience™ (Invitrogen™)

Amphiregulin Monoclonal Antibody for Flow

Amphiregulin Monoclonal Antibody (AREG559), PE, eBioscience™ (Invitrogen™)

Amphiregulin Monoclonal Antibody for Flow

Amphiregulin Monoclonal Antibody (AREG559), eBioscience™ (Invitrogen™)

Amphiregulin Monoclonal Antibody for Western Blot, Flow

Androgen Receptor Monoclonal Antibody (AR 441), Biotin (Invitrogen™)

Androgen Receptor Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

Androgen Receptor Monoclonal Antibody (AR 441) (Invitrogen™)

Androgen Receptor Monoclonal Antibody for Western Blot, IF, ICC, IHC (P), IP

AR Monoclonal Antibody (1F7C12) (Proteintech)

AR Monoclonal Antibody for Western Blot, IHC (P)

AR Monoclonal Antibody (OTI2E6), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot, IHC (P)

AR Monoclonal Antibody (OTI2E11), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot, IHC (P)

AR Monoclonal Antibody (OTI2E11), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot, IHC (P)

AR Monoclonal Antibody (OTI2H2), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot

AR Monoclonal Antibody (OTI3C5), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot, IHC (P)

AR Monoclonal Antibody (OTI1D3), TrueMAB™ (OriGene)

AR Monoclonal Antibody for Western blot, IHC (P)