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Phospho-ATF2 (Tyr69, Thr71) Recombinant Polyclonal Antibody (24HCLC) (Invitrogen™)

Phospho-ATF2 (Tyr69, Thr71) Recombinant Polyclonal Antibody for Western Blot, IF, ICC

LanthaScreen™ ATF2 (19-106) A549 Cell Line

The LanthaScreen®ATF2 (19-106) A549 cell line contains a fusion protein consisting of GFP and a fragment encoding for aa 19-106 of ATF2 under the control of a CMV promotor. A549 is a lung carcinoma cell line which shows inducible activation of JNK by number of different ligands, such as TNF and EGF, which leads to the transient phosphorylation of GFP-ATF2 (19-106). This LanthaScreen®cell line therefore allows the analysis of JNK activity using GFP-ATF2 phosphorylation as readout. The GFP-ATF2 (19-106) construct was transfected into A549 cells using Lipofectamine™2000, followed by selection with Blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for JNK mediated GFP-ATF2 (19-106) phosphorylation. This cell line has been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time. Additional information using alternate stimuli is also available. Academic and non-profit customers, please inquire for special pricing.

Phospho-ATF2 (Thr71) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr71) Polyclonal Antibody for Western Blot, IHC (P)

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

GFP-ATF2 (19-96)

A truncated version (residues 19-96) of ATF2 has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK and p38 families of kinases.

For use in combination with the LanthaScreen® Tb-anti-ATF2 [pThr71] antibody (PV4451) to evaluate inhibitors in medium to high throughput screening applications.

Phospho-ATF2 (Thr69) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr69) Polyclonal Antibody for Western Blot, IHC (P), IP

Phospho-ATF2 (Thr73) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr73) Polyclonal Antibody for Western Blot, IHC (P), IP

ATF2 Monoclonal Antibody (4F12) (Invitrogen™)

ATF2 Monoclonal Antibody for Western Blot, IHC

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, pep-ELISA

Phospho-ATF2 (Ser480) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser480) Polyclonal Antibody for Western Blot, IF, IHC (P)

Phospho-ATF2 (Ser322) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser322) Polyclonal Antibody for DB

Phospho-ATF2 (Ser94, Ser112) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser94, Ser112) Polyclonal Antibody for Western Blot, IHC (P)

Phospho-ATF2 (Thr51, Thr69) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr51, Thr69) Polyclonal Antibody for Western Blot, IF, ICC, IHC (P)

Phospho-ATF2 (Ser472) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser472) Polyclonal Antibody for IHC (P)

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, IHC (P), IP

Phospho-ATF2 (Thr55, Thr73) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr55, Thr73) Polyclonal Antibody for Western Blot, IHC (P), IP

Phospho-ATF2 (Thr52) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr52) Polyclonal Antibody for Western Blot

Phospho-ATF2 (Ser490, Ser498) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser490, Ser498) Polyclonal Antibody for Western Blot, IHC

Phospho-ATF2 (Thr53, Thr71) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr53, Thr71) Polyclonal Antibody for Western Blot, IHC (P)

Phospho-ATF2 (Ser44, Ser62) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser44, Ser62) Polyclonal Antibody for Western Blot, IHC (P)

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, IHC (P), IP

Phospho-ATF2 (Ser498) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Ser498) Polyclonal Antibody for Western Blot, IHC (P)

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for IHC (P)

Phospho-ATF2 (Thr71) Monoclonal Antibody (H.826.8) (Invitrogen™)

Phospho-ATF2 (Thr71) Monoclonal Antibody for Western Blot, Flow

Phospho-ATF2 (Thr71) Monoclonal Antibody (103C411.2) (Invitrogen™)

Phospho-ATF2 (Thr71) Monoclonal Antibody for Western Blot, Flow

Phospho-ATF2 (Thr69, Thr71) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr69, Thr71) Polyclonal Antibody for Western Blot, IHC (P)

Phospho-ATF2 (Thr71) Polyclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr71) Polyclonal Antibody for Western Blot, IF, ICC, IHC (F), IHC (P), Flow, IP

ATF2 Monoclonal Antibody (S.613.5) (Invitrogen™)

ATF2 Monoclonal Antibody for Western Blot, IHC (P), IP

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, Flow

ATF2 Polyclonal Antibody (Proteintech)

ATF2 Polyclonal Antibody for Western Blot, IF, ICC, IP

ATF2 Polyclonal Antibody (Bethyl Laboratories)

ATF2 Polyclonal Antibody for IP

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (SC05-90) (Invitrogen™)

Phospho-ATF2 (Thr71) Recombinant Monoclonal Antibody for Western Blot, IF, ICC

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (ATF2T71-G3), APC (Invitrogen™)

Phospho-ATF2 (Thr71) Recombinant Monoclonal Antibody for Flow

ATF2 Polyclonal Antibody (Bethyl Laboratories)

ATF2 Polyclonal Antibody for Western Blot, IP

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (ATF2T71-G3) (Invitrogen™)

Phospho-ATF2 (Thr71) Recombinant Monoclonal Antibody for Western Blot, Flow

ATF2 Recombinant Rabbit Monoclonal Antibody (SZ17-01) (Invitrogen™)

ATF2 Recombinant Monoclonal Antibody for Western Blot, IF, ICC, IHC

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (Invitrogen™)

Phospho-ATF2 (Thr71) Recombinant Monoclonal Antibody for Western Blot, IF, ICC, ELISA

ATF2 Polyclonal Antibody (Invitrogen™)

ATF2 Polyclonal Antibody for Western Blot, IF, ICC, IHC (F), IHC (P), Flow

ATF2 Polyclonal Antibody (Bethyl Laboratories)

ATF2 Polyclonal Antibody for Western Blot

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (ATF2T71-G3), FITC (Invitrogen™)

Phospho-ATF2 (Thr71) Recombinant Monoclonal Antibody for Flow

ATF2 Recombinant Rabbit Monoclonal Antibody (Invitrogen™)

ATF2 Recombinant Monoclonal Antibody for Western Blot, IHC (P), ELISA

ATF2 Polyclonal Antibody (Proteintech)

ATF2 Polyclonal Antibody for Western Blot, IHC (P)

GDNF Human ELISA Kit (Invitrogen™)

GDNF Human ELISA Kit for ELISA

Streptavidin, Alexa Fluor™ 647 conjugate (Invitrogen™)

Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor® 647 Streptavidin Conjugates:
Alexa Fluor® 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor® Dyes
Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

Learn more about Alexa Fluor® Dyes

Find out about other Labeled Streptavidin Conjugates

Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Human Glial Cell Derived Neurotrophic Factor (GDNF) Recombinant Protein (Invitrogen™)

Human Glial Cell Derived Neurotrophic Factor (GDNF) Recombinant Protein for Ctrl

GDNF Polyclonal Antibody (Invitrogen™)

GDNF Polyclonal Antibody for Western Blot, ELISA, Neu, IA

GDNF Polyclonal Antibody (Invitrogen™)

GDNF Polyclonal Antibody for Western Blot

GDNF Polyclonal Antibody (Invitrogen™)

GDNF Polyclonal Antibody for Western Blot, IHC, ELISA

GDNF Polyclonal Antibody (Invitrogen™)

GDNF Polyclonal Antibody for Western Blot

GDNF Polyclonal Antibody (Invitrogen™)

GDNF Polyclonal Antibody for Western Blot, IHC