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AXL [R499C] Recombinant Human Protein

AXL, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase which is a member of the TAM (TYRO3/AXL/MER) receptor tyrosine kinase subfamily. AXL represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats and Axl transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. AXL is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Recombinant human AXL R499C (473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

AXL Human ELISA Kit (Invitrogen™)

AXL Human ELISA Kit for ELISA

Axl Monoclonal Antibody (DS7HAXL), Super Bright 436, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Axl Antibody (15H4L2), ABfinity™ Rabbit Monoclonal (Invitrogen™)

Axl Monoclonal Antibody for Western Blot

AXL Human ELISA Kit (Invitrogen™)

AXL Human ELISA Kit

Axl Antibody (2H29L9), ABfinity™ Rabbit Monoclonal (Invitrogen™)

Axl Monoclonal Antibody for IF, ICC

Axl Monoclonal Antibody (MAXL8DS), PE, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Axl Monoclonal Antibody (MAXL8DS), PE-Cyanine7, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

AXL Recombinant Human Protein (Invitrogen™)

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation.

The purified recombinant protein can be used in a number of different applications, but it has been specifically validated for use with both the LanthaScreen™ & Z'-LYTE™ assay technologies to identify novel kinase inhibitors. AXL is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the LanthaScreen & Z'-LYTE assay technology
• Available in multiple pack sizes for convenient use
• Also available in our SelectSceen Kinase Profiling Service

Axl Monoclonal Antibody (DS7HAXL), PE, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Axl Monoclonal Antibody (DS7HAXL), PE-Cyanine7, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Axl Monoclonal Antibody (MAXL8DS), APC, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

AXL Mouse ELISA Kit (Invitrogen™)

Axl Mouse ELISA Kit for ELISA

AXL Human ProcartaPlex™ Simplex Kit (Invitrogen™)

The Human AXL Simplex ProcartaPlex kit measures AXL protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 µL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.

ProcartaPlex Simplex kits are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information, including a comprehensive list of individual protein targets.

Reactivity/species: human
Suitable sample types: cell culture supernatant, serum, plasma
Sample volume: serum, plasma: 25 µL; CCS: 50 µL
Reported application: multiplex immunoassay

Axl Monoclonal Antibody (MAXL8DS), Alexa Fluor 488, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Axl Monoclonal Antibody (DS7HAXL), APC, eBioscience™ (Invitrogen™)

Axl Monoclonal Antibody for Flow

Phospho-Axl (Tyr691) Polyclonal Antibody (Invitrogen™)

Phospho-Axl (Tyr691) Polyclonal Antibody for Western Blot

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC

AXL Monoclonal Antibody (3) (Invitrogen™)

AXL Monoclonal Antibody for ELISA

Phospho-Axl (Tyr691) Polyclonal Antibody (Invitrogen™)

Phospho-Axl (Tyr691) Polyclonal Antibody for Western Blot, IHC (P)

Phospho-Axl (Tyr779) Monoclonal Antibody (713610) (Invitrogen™)

Phospho-Axl (Tyr779) Monoclonal Antibody for Western Blot, IF, ICC, Flow

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC, Flow

Axl Monoclonal Antibody (1B3A2) (Invitrogen™)

Axl Monoclonal Antibody for Western Blot

Phospho-Axl (Tyr702) Polyclonal Antibody (Invitrogen™)

Phospho-Axl (Tyr702) Polyclonal Antibody for Western Blot

Axl Monoclonal Antibody (7E10) (Invitrogen™)

Axl Monoclonal Antibody for Western Blot, IF, ICC

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC (P)

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC (P), ELISA

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC (P)

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot

Axl Monoclonal Antibody (E.614.5) (Invitrogen™)

Axl Monoclonal Antibody for Western Blot

Axl Polyclonal Antibody (Invitrogen™)

Axl Polyclonal Antibody for Western Blot, IHC (P)

Axl Monoclonal Antibody (C.995.0) (Invitrogen™)

Axl Monoclonal Antibody for Western Blot, Flow, IP

AXL Polyclonal Antibody (Proteintech)

AXL Polyclonal Antibody for Western Blot, Flow, IP

Axl Monoclonal Antibody (C4-A8) (Invitrogen™)

Axl Monoclonal Antibody for Western Blot, ICC

Axl Polyclonal Antibody (Bethyl Laboratories)

Axl Polyclonal Antibody for Western Blot

TaqMan™ Array Human Protein Kinase Panel (Applied Biosystems™)

The Applied Biosystems® TaqMan® Human Protein Kinase Array is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of human protein kinase genes important in drug discovery. This product is designed with TaqMan® Gene Expression Assays arrayed in singletons in 384 wells and is compatible with an upgraded Applied Biosystems 7900HT Fast Real-Time PCR System.

The Human Protein Kinase Array contains assays for 68 human kinases. Fifty-three of the 68 kinases are from receptor protein-tyrosine kinase (RPTK) families: EGFR, InsulinR, PDGFR, VEGFR, FGFR, CCK, NGFR, HGFR, EPHR, AXL, TIE, RYK, DDR, RET, ROS, LTK, ROR and MUSK. The remaining 15 kinases are Ser⁄Thr kinases from the kinase families: CAMKL, IRAK, Lmr, RIPK and STKR. We have also selected assays for 26 classic endogenous, non-kinase genes. These genes are involved in signal transduction and mediate protein-protein interaction, transcriptional regulation, neural development and cell adhesion. This product is sold in packages of 4 cards.

The array is pre-formatted and inventoried, cost-effective, convenient and easy to use without needing expensive robotics. Results are reproducible and consistent across samples, studies, and labs —the same data quality from card-to-card and lot-to-lot — even with different operators.