Polyacrylamide gel electrophoresis provides very high resolution of DNA molecules 10–3,000 bp long. Under the appropriate conditions, DNA molecules differing in size by only a single base pair can be resolved (learn more: Nucleic acid electrophoresis education ).
In the nucleic acid electrophoresis workflow, several steps are performed sequentially, each of which could impact the outcome of nucleic acid separation. This article addresses five considerations associated with samples, reagents, and running parameters.
Gel electrophoresis is a common laboratory technique to isolate nucleic acids and proteins. Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. Gel electrophoresis is vital in all molecular biology workflows.
DNA electrophoresis is a standard laboratory technique used to identify, quantify, and purify DNA fragments. DNA electrophoresis involves loading DNA samples into the wells of an agarose or acrylamide gel and subjecting it to an electric field.
Electrophoresis is a common lab technique used to identify, quantify, and purify nucleic acid fragments and assess quality. RNA molecules are negatively charged, and during gel electrophoresis they migrate toward the anode in the presence of an electric current.
See all protein-nucleic acid interaction products, Introduction to the EMSA (gel shift) technique Target probes Labeling and detection Nonspecific and specific competitors Binding reaction components Electrophoresis Supershift assays Recommended reading, Invitrogen Custom DNA Oligos Pierce RNA 3'...
Explore: Protein electrophoresis products Download: Protein Electrophoresis Handbook, Protein electrophoresis is a standard laboratory technique by which charged protein molecules are transported through a solvent by an electrical field.
Most life science researchers will run a gel at some point in their careers. Running a gel, formally called gel electrophoresis, is an effective method to separate biomolecules, such as nucleic acids and proteins , based on size.
NuPAGE Bis-Tris Gels and Bolt Bis-Tris Plus Gels are high-performance precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. The neutral pH Bis-Tris chemistry delivers consistent gel performance and minimizes protein...
Electrophoresis underpins many molecular biology applications. Therefore, problems in nucleic acid gel electrophoresis hinders downstream applications and hampers experimental workflow; often errors in gel electrophoresis negatively impact the results of an experiment.
Pour your own polyacrylamide mini gels easily and confidently with the Invitrogen SureCast Gel Handcast System. Request a demo Leak-free* design Durable glass plates Single component assembly using a single-motion load-and-lock mechanism Unique tilt feature- minimizes spillage during gel casting,...
For Research Use Only. Not for use in diagnostic procedures., View all Can I prepare my protein sample with the reducing agent and store it for future use? Can reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis?
We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios., View the relevant topics below: General 1D Electrophoresis Bolt™ Bis –Tris Plus Gels NuPAGE™ Bis-Tris and NuPAGE™ Tris-Acetate Gels Novex™ Tris-Glycine Gels Thermo...
We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios. View the relevant questions below:, Browse our FAQ database for more information ›, Need more information? Contact us ›, For Research Use Only.
(See a list of the products featured in this article.) Polyacrylamide gel electrophoresis (PAGE)—an important technique for separating proteins based on their electrophoretic mobility—is widely used for both protein analysis and protein purification.