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Is CytoVista 3D Cell Culture Clearing Reagent (Cat. No. V11315, V11326) compatible with 3D cell culture matrices like Matrigel matrix (Corning)? Product FAQ

Answer

No. CytoVista 3D Cell Culture Clearing Reagent (Cat. No. V11315, V11326) is incompatible with most 3D cell culture matrices. We recommend harvesting the cells from the matrix to enhance the penetration of antibodies and other staining reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E21444

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What reagents do you offer for cell dissociation, and what are the main differences between them? Product FAQ

Answer

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Answer Id: E11900

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What trypsin concentration should be used to dissociate cells? Product FAQ

Answer

The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco Trypsin solutions and recommended dissociation conditions here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/trypsin.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E13274

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Do I have to use CytoVista 3D Cell Culture Clearing Reagent under a chemical safety hood? Product FAQ

Answer

We recommend following the usage guidelines specified in the MSDS.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Answer Id: E19100

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What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme? Product FAQ

Answer

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Answer Id: E11902

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Will improper storage of my tissue culture reagents affect the growth rate of my culture? Product FAQ

Answer

Yes. Store animal sera at -5 to -20 degrees C. Store media at 2 to 8 degrees C; use within recommended shelf life period. Store complete media (supplemented) at 2 to 8 degrees C, and for complete medium the recommended shelf life is 2 to 4 weeks. Additionally, minimize exposure of sera and media to light.

Answer Id: E11825

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What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth? Product FAQ

Answer

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11953

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What is the difference between Gibco GlutaMAX and glutamine supplements? Product FAQ

Answer

GlutaMAX-I supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture medium. Please go here to read more about the properties of GlutaMAX supplement (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/media-supplements/glutamax-media/glutamax-vs-glutamine.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E11884

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Do I need to add additional supplements to AmnioMAX-II Complete Medium (Cat. No. 11269016)? Product FAQ

Answer

No. AmnioMax-II Complete Medium (Cat. No. 1129016) is fully supplemented with antibiotics, L-glutamine, and FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E21694

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Can Gibco RevitaCell Supplement be used in addition to a traditional ROCK Inhibitor? Product FAQ

Answer

No. Combination of the Gibco RevitaCell Supplement with a traditional ROCK inhibitor results in deleterious effects on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E12458

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Can RevitaCell Supplement be used in addition to a traditional ROCK Inhibitor? Product FAQ

Answer

No. Combination of RevitaCell Supplement with a traditional ROCK inhibitor will result in deleterious effects on PSCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E17256

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At what points in induced pluripotent stem (iPS) cell culture should CTS RevitaCell Supplement (100X) (Cat. No. A4238401) be used? Product FAQ

Answer

CTS RevitaCell Supplement (100X) should be used in combination with cell culture medium as a post-thaw recovery solution to improve cell viability. It can also be used in combination with CTS Essential 8 Medium (Cat. No. A2656101) when performing single cell passaging procedures.

Find additional tips, troubleshooting help, and resources within our Stem Cell Culture Support Center

Answer Id: E22301

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Do both the DMEM, high glucose, GlutaMAX Supplements, Cat. No. 61965026 and 10566016, have the same formulation? Product FAQ

Answer

Yes, both the DMEM, high glucose, GlutaMAX Supplements, Cat. No. 61965026 and 10566016, have the same formulation.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Answer Id: E21397

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Which cells can I use StemPro Neural Supplement with? Product FAQ

Answer

StemPro Neural Supplement can be used with human and rat NSCs, GPCs, and some neuronal progenitors.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E18625

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Is there a difference between the Gibco N-2 Supplement formulation from Thermo Fisher and the N-2 Supplement formulation from Global Stem? Product FAQ

Answer

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Answer Id: E20521

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