Product FAQ

Can I run my E-Gel Agarose Gel longer than recommended?

Answer

Do not run the gels longer than 40 minutes for the single comb gels or longer than 20 minutes for the double comb gels as ions get depleted and longer run times will damage the gel. Do not run E-Gel 48 Agarose Gels longer than 30 minutes or E-Gel 96 Agarose Gels longer than 20 minutes.

Answer Id: E8014

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Product FAQ

I am seeing multiple DNA bands after running a single vector on my agarose gel. Why is this?

Answer

The conformation of plasmid DNA will affect its mobility on a gel. Plasmid DNA can be supercoiled (native 3D conformation), nicked circular (nick in one DNA strand) or linear (nicks in both DNA strands). You may see all three forms on an agarose gel. Supercoiled DNA runs the fastest and will show up lowest on the gel, linear DNA runs in the middle of the gel, and nicked circular DNA shows up at the top of the gel, since it migrates the slowest.

Answer Id: E8015

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Citations & References

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

  • Authors: McMaster GK, Carmichael GG
  • Journal: Proc Natl Acad Sci U S A (1977) 74:4835-4838
  • PubMed ID: 73185

Product FAQ

How do E-Gel 48 Agarose Gels and E-Gel 96 Agarose Gels work?

Answer

E-Gel 48 and E-Gel 96 gels contain a proprietary neutral-pH internal buffer system with special high capacity and low conductivity features. There are no ion exchange matrices.

Answer Id: E7971

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Citations & References

Real-time detection of allele-specific polymerase chain reaction products by automated ultra-thin-layer agarose gel electrophoresis.

  • Authors: Guttman A, Barta C, Szöke M, Sasvári-Székely M, Kalász H
  • Journal: J Chromatogr A (1998) 828:481-487
  • PubMed ID: 9916326
Catalog #

Product FAQ

I accidently stored my E-Gel Agarose Gels at 4°C instead of room temperature. Can I still use them?

Answer

While we recommend storage at room temperature, these gels will still be usable. Bring the gels to room temperature prior to the run for optimal conditions.

Answer Id: E8016

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Product Literature

Flyer: E-Gel CloneWell II precast agarose gels

Manual / Product Insert

Quick Reference: E-Gel SizeSelect Agarose Gel (Japanese)

Version: EGel SizeSelect Agarose Gel_QRC_J1_revA.doc
Catalog #
  • G661002(Discontinued)
  • G6612STEU(Discontinued)
  • G6612STUK(Discontinued)
  • G6612ST(Discontinued)

Product FAQ

I’m trying to anneal my oligos to create a ds oligo for ligation into one of your shRNA or miRNA RNAi vectors. When I run my ligated ds oligo on an agarose gel, the bands are weak. What could be happening?

Answer

Please review the possibilities below:

- Single-stranded oligos designed incorrectly; verify that the sequence of the bottom-strand oligo is complementary to the sequence of the top strand oligo.
- Ensure that oligos are annealed at room temp for 5-10 minutes after heating to 95 degrees C.
- Check the molar ratio you are using for annealing top and bottom-strand oligo; equal amounts should be used.

Answer Id: E10016

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Manual / Product Insert

Quick Ref: E-Gel® EX Agarose Gel

Version: Rev date: 6 August 2009
Catalog #
  • G401001
  • G401002
  • G401004
  • G402001
  • G402002
  • G6511STEU(Discontinued)
  • G6511STUK(Discontinued)
  • G6511ST(Discontinued)
  • G6512STEU(Discontinued)
  • G6512STUK(Discontinued)
  • G6512ST(Discontinued)

Product FAQ

I am getting poor resolution or see smearing of bands when using my E-Gel EX Agarose Gels. Why is this?

Answer

Here are some possibilities and suggestions to resolve the problem:

- The sample is overloaded. Do not load more than the recommended amount.
- A high salt concentration. Dilute the samples.
- The E-Gel EX Agarose Gels were prerun; this is not recommended for these gels.
- A very low volume of sample was loaded or the sample was not loaded properly.
- Bubbles may have been introduced while loading the samples. Bubbles will cause band distortion. Load the recommended sample volume based on the gel type and loading method. For proper band separation, we recommend keeping sample volumes uniform. Load deionized water or TE into any empty wells, and avoid introducing bubbles.
- The gel was not electrophoresed immediately after sample loading. Run your sample within 1 minute of loading it.
- The E-Gel Agarose Gel may have been used beyond its expiration date. Check the expiration date.
- A longer electrophoresis run time or high current was used during the run. Longer run times cause an increase in the current, resulting in poor band migration or a melted gel. Do not run the gel longer than the recommended time for each gel type.

Answer Id: E8023

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Limited Use Label License

E-Gel™ CloneWell™ Agarose Gels Starter Kit, 0.8% (China)

Limited Use Label License
394
Catalog #
  • G6500STCH(Discontinued)

Product FAQ

Can I use agarose gels or must I use acrylamide gels for the LightShift Chemiluminescent EMSA Assay?

Answer

EMSAs may be performed with either polyacrylamide or agarose gels depending upon the resolution requirements of the study system. Traditionally, 4-6% non-denaturing polyacrylamide gels are used. If agarose is used, a capillary transfer may be best. Either capillary or electrical transfers can be performed with polyacrylamide gels.

Answer Id: E15490

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Citations & References

Measurement of electrophoretic mobility of dye-labeled large DNA fragments in agarose gels by movement of fluorescence pattern after photobleaching.

  • Authors: Chu B, Wang ZL, Wu C
  • Journal: Biopolymers (1989) 28:1491-1494
  • PubMed ID: 2752103
Catalog #
  • E1305(Discontinued)
  • E3565(Discontinued)

Product FAQ

Can agarose gels be cast with SYBR Safe DNA Gel Stain in them?

Answer

Yes. Simply substitute a SYBR Safe DNA Gel Stain solution for the buffer when preparing the molten agarose. If you are using the 10,000X SYBR Safe DNA Gel Stain concentrate, dilute the concentrated stain 1:10,000 in agarose gel buffer (e.g., 1X TBE or 1X TAE) and add the buffer/stain solution to the powdered agarose. You can heat the agarose/SYBR Safe DNA Gel Stain mixture briefly in the microwave.

Answer Id: E8049

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