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Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells. Citations & References

  • Authors: Singh U, Quintanilla RH, Grecian S, Gee KR, Rao MS, Lakshmipathy U,
  • Journal: Stem Cell Rev (2012) 0:-
  • PubMed ID: 22426885
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Stability of nitroblue tetrazolium-based alkaline phosphatase substrates. Citations & References

  • Authors: Smejkal GB, Kaul CA
  • Journal: J Histochem Cytochem (2001) 49:1189-1190
  • PubMed ID: 11511689
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Novel Fluorogenic Alkaline Phosphatase Substrate Used for Detection of Endrogenous Enzymatic Activity. Citations & References

  • Authors: Paragas V, et al.
  • Journal: Mol Biol Cell 1993; ((Suppl 4)):na 226a, abstract #1312-226a, abstract #1312
  • PubMed ID: 0
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The ELF-97 alkaline phosphatase substrate provides a bright, photostable, fluorescent signal amplification method for FISH. Citations & References

  • Authors: Paragas VB, Zhang YZ, Haugland RP, Singer VL
  • Journal: J Histochem Cytochem (1997) 45:345-357
  • PubMed ID: 9071316
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How does Alkaline Phosphatase Live Stain compare to standard AP assays? Product FAQ


Unlike traditional alkaline phosphatase staining assays, which are terminal, the Alkaline Phosphatase Live Stain allows you to visualize your pluripotent stem cell colonies without destroying your cells. The alkaline phosphatase substrate in the Alkaline Phosphatase Live Stain is non-toxic to cells and diffuses out in two hours.

Answer Id:: E12557

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What are the Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB)? Product FAQ


- Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB) are ready-to-use, single component substrates for sensitive immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. The sensitivity of the AP substrate is in the 100 pg range, while the HRP substrate is in the 1 ng range (see Note below).

- Invitrogen AP Chromogenic Substrate consists of a ready-to-use solution of 5-bromo-4-chloro-3-indolyl-1-phosphate (BCIP) and nitroblue tetrazolium (NBT) which forms a black-purple precipitate upon reaction with alkaline phosphatase. It is available as a standalone product (Cat. Nos. WP20001) and is also a component of the WesternBreeze Chromogenic Detection Kits.

- Invitrogen HRP Chromogenic Substrate consists of a ready-to-use non-toxic solution of 3,3',5,5'-tetramethylbenzidine (TMB) which forms a blue precipitate upon reaction with horse radish peroxidase. It is available as a standalone product (Cat. No. WP20004).

Note: Sensitivity may vary depending upon the antigen and antibody used for detection.

Answer Id:: E10833

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What substrates are compatible with the Clean-Blot IP Detection reagent? Product FAQ


For best results, use the the Clean-Blot IP Detection with SuperSignal Substrates or Thermo Scientific ECL Western Blotting Substrate. When using other HRP or AP substrates, empirically determine the optimal concentration of the Clean-Blot IP Detection Reagent.

Answer Id:: E8413

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New chromogens for alkaline phosphatase histochemistry: salmon and magenta phosphate are useful for single- and double-label immunohistochemistry. Citations & References

  • Authors: Avivi C, Rosen O, Goldstein RS
  • Journal: J Histochem Cytochem (1994) 42:551-554
  • PubMed ID: 8126379

Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate. Citations & References

  • Authors: Telford WG, Cox WG, Stiner D, Singer VL, Doty SB
  • Journal: Cytometry (1999) 37:314-319
  • PubMed ID: 10547617
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Spatial patterns of alkaline phosphatase expression within bacterial colonies and biofilms in response to phosphate starvation. Citations & References

  • Authors: Huang CT, Xu KD, McFeters GA, Stewart PS
  • Journal: Appl Environ Microbiol (1998) 64:1526-1531
  • PubMed ID: 9546188
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Phosphate stress in cultures and field populations of the dinoflagellate prorocentrum minimum detected by a single-cell alkaline phosphatase assay Citations & References

  • Authors: Dyhrman ST, Palenik B
  • Journal: Appl Environ Microbiol (1999) 65:3205-3212
  • PubMed ID: 10388722
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A high-affinity reversible protein stain for Western blots. Citations & References

  • Authors: Antharavally BS, Carter B, Bell PA, Krishna Mallia A
  • Journal: Anal Biochem 2004; (329):2 276-280
  • PubMed ID: 15158487
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Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples. Citations & References

  • Authors: Zerbini M, Cricca M, Gentilomi G, Venturoli S, Gallinella G, Musiani M
  • Journal: Clin Chim Acta (2000) 302:79-87
  • PubMed ID: 11074066
Catalog # T24831(Blocked)

Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation. Citations & References

  • Authors: Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T
  • Journal: J Bone Miner Res (2006) 21:1012-1021
  • PubMed ID: 16813522

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