Documents & Support

1 - 15 of 242 results

Renilla luciferase- Aequorea GFP (Ruc-GFP) fusion protein, a novel dual reporter for real-time imaging of gene expression in cell cultures and in live animals. Citations & References

  • Authors: Wang Y, Yu YA, Shabahang S, Wang G, Szalay AA
  • Journal: Mol Genet Genomics
  • PubMed ID: 12395190
Catalog #
  • C2944
  • C6777(Discontinued)

Pluripotent and Metabolic Features of Two Types of Porcine iPSCs Derived from Defined Mouse and Human ES Cell Culture Conditions. Citations & References

  • Authors: Zhang W, Pei Y, Zhong L, Wen B, Cao S, Han J
  • Journal: PLoS One
  • PubMed ID: 25893435

Are the quantum dots toxic? Product FAQ

Answer

We have not investigated the toxicity of the Qdot nanocrystals. The materials are provided in a solution which is approximately 2 mM total Cd concentration. We have demonstrated the utility of these materials in a variety of live-cell in vitro labeling experiments, but do not have systematic data investigating the toxicity of the materials to humans, to animals, or to cells in culture.

Answer Id:: E12232

Was this answer helpful?

Yes No

Thank you for your response

What is BVD virus (BVDV) in fetal bovine serum and how does it apply to cell culture applications? Product FAQ

Answer

BVD stands for Bovine Viral Diarrhea. It is one of the most common viral infections in cattle. It is estimated that 70 to 90 percent of the world's cattle population is seropositive for BVD. This virus can cause abnormalities and fetal abortions in cattle. Several strains of BVD exist, some of which are non-pathogenic.

Bovine serum is tested in accordance with 9 CFR, Section 113.53. The BVDV fluorescent antibody test is one of the required tests to meet Title 9 part 113.53 of the Code of Federal Regulations. However, the results of this test are somewhat subjective in the way they are scored. They are scored 0 to +4, based on the level of observed fluorescence. Most samples will have a detectable level of BVDV antigen due to its prevalence in the bovine population. The FA part of the testing can reveal the presence of non-cytopathic BVD strains. The Cytopathogenic and Hemadsorbing Agents testing is used to determine the release of bovine serum, regardless of the BVDV result. We currently report the BVDV results as “Tested”. Any live cytopathic bovine viruses (including the cytopathic BVDV strain) would be revealed in the testing for cytopathogenic agents. In this portion of the testing, the cultures are microscopically monitored for evidence of inclusion bodies, abnormal number of giant cells, or other cytopathology indicative of cell abnormalities. The hemadsorption assay detects the presence of hemagglutinating viruses. The viral hemagglutinin would induce clumping of red blood cells. If a positive result in the cytopathogenic agents or hemadsorbing agents assays is reported, the material would be failed and therefore would not be released by Quality Assurance.

Answer Id:: E11874

Was this answer helpful?

Yes No

Thank you for your response

What is the importance of gamma-irradiated sera? Product FAQ

Answer

Gamma irradiation is recognized as an effective method for inactivating viruses in animal-origin material. Based on USDA regulations for the general requirements for antibody products (9CFR, Section 113.450), the minimum dosage for blood derivatives of animal origin is 25 kGy. Certain European countries require products to be treated prior to importation with a minimum dose of 25 kGy. We will gamma-irradiate serum on request. We have validated a process for utilizing gamma irradiation in the range of 30-45 kGy to inactivate the most common bovine viruses and mycoplasmas that may be present in FBS. The level of inactivation is 6-8 logs for viruses and 6-7 logs for mycoplasmas. We have also demonstrated that physiochemical properties and cell culture performance of serum is not altered by gamma irradiation at levels of 30-45 kGy.

Answer Id:: E11869

Was this answer helpful?

Yes No

Thank you for your response

Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Citations & References

  • Authors: De Melo N, McGinlay S, Markus R, Macri-Pellizzeri L, Symonds ME, Ahmed I, Sottile V
  • Journal: Biomimetics (Basel)
  • PubMed ID: 31295946

Feeder layer- and serum-free culture of human embryonic stem cells. Citations & References

  • Authors: Amit M, Shariki C, Margulets V, Itskovitz-Eldor J,
  • Journal: Biol Reprod
  • PubMed ID: 14627547
Catalog #

Cell fusion-independent differentiation of neural stem cells to the endothelial lineage. Citations & References

  • Authors: Wurmser AE, Nakashima K, Summers RG, Toni N, D'Amour KA, Lie DC, Gage FH
  • Journal: Nature
  • PubMed ID: 15254537
Catalog #

3D imaging of Sox2 enhancer clusters in embryonic stem cells. Citations & References

  • Authors: Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R,
  • Journal: Elife
  • PubMed ID: 25537195

Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy compliant human adipose stem cells. Citations & References

  • Authors: Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerström B, Miettinen S,
  • Journal: Stem Cell Res Ther
  • PubMed ID: 23497764

Long-term live imaging provides new insight into stem cell regulation and germline-soma coordination in the Drosophila ovary. Citations & References

  • Authors: Morris LX, Spradling AC,
  • Journal: Development
  • PubMed ID: 21558370

Self-Assembling Peptide Amphiphile Nanofibers as a Scaffold for Dental Stem Cells Citations & References

  • Authors: Galler, KM; Cavender, A; Yuwono, V; Dong, H; Shi, ST; Schmalz, G; Hartgerink, JD; D'Souza, RN
  • Journal: Tissue Engineering Part A
Catalog #

Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. Citations & References

  • Authors: Albert R, Veréb Z, Csomós K, Moe MC, Johnsen EO, Olstad OK, Nicolaissen B, Rajnavölgyi E, Fésüs L, Berta A, Petrovski G
  • Journal: PLoS One
  • PubMed ID: 23056608

Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate into sweat gland-like cells: a preclinical study. Citations & References

  • Authors: Yang S, Ma K, Feng C, Wu Y, Wang Y, Huang S, Fu X,
  • Journal: Front Med
  • PubMed ID: 23794058

Stem cell characteristics of amniotic epithelial cells. Citations & References

  • Authors: Miki T, Lehmann T, Cai H, Stolz DB, Strom SC
  • Journal: Stem Cells
  • PubMed ID: 16081662
Results per page
spinner