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VersaTREK Detection IFU Manual / Product Insert

VersaTREK Detection IFU Manual / Product Insert

Induction of nitric oxide in human monocytes and monocyte cell lines by Mycobacterium tuberculosis. Citations & References

  • Authors: Jagannath C, Actor JK, Hunter RL
  • Journal: Nitric Oxide
  • PubMed ID: 9731635
Catalog #
  • G7921
  • N7927(Discontinued)
  • D7918(Discontinued)
  • N7892(Discontinued)

How do you run a dose response curve for Geneticin (G418) or other selective antibiotic? Product FAQ

Answer

The amount of antibiotic required to be present in culture media to select for resistant cells varies with a number of factors, including cell type. Good laboratory practice requires that the optimal concentration of Selective Antibiotic required to maintain and select cells must be determined for each set of growth conditions. Whenever experimental conditions are altered (including use of Selective Antibiotic from a different lot), the optimal concentration of the product should be re-evaluated.

Below is a brief protocol for performing a kill curve with Geneticin. Follow the general protocol for other antibiotics as well but use appropriate ranges for each antibiotic. For example, Geneticin: 100-1,500 ug/ml, Blasticidin: 1-10ug, Zeocin resistance gene: 100-1000 ug/ml.

Kill Curve Assay:

1. Dissolve Geneticin Selective Antibiotic in fully supplemented growth medium without antibiotics at a concentration of 5 mg/ml and filter using a 0.22 micron filter.
2. Prepare 6-well cell culture plates by adding Geneticin Selective Antibiotic to the growth medium to desired concentrations. A range from 100-1,200 µg/ml in 100 µg increments is recommended.
3. Treat cells with trypsin and dilute to a concentration of 4000 cells/ml.
4. Add 100 µl of cell suspension to each well and incubate plates in a humidified CO2 atmosphere at 37°C.
5. At 10 to 14 days, aspirate the supernatant and wash the cells with phosphate buffered saline and stain the cells with 0.5% methylene blue and 50% methanol for 20 minutes.
6. Score the plates by calculating percentage of survival by number of individual colonies for percent confluence.
7. Calculate the percentage of survival in the presence of each dilution of Geneticin Selective Antibiotic versus the percentage of survival in the absence of Geneticin.
8. Generate a dose response curve by plotting the percentage of survival on the y axis versus the concentration of Geneticin Selective Antibiotic in µg/ml on the x axis for both the sensitive and resistant cell lines.

If you are performing sequential transfections, one needs to establish dose response curve for the first antibiotic, create a stable and then perform a second does response curve on that stable in the presence of 2 antibiotics.

Answer Id:: E4300

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Clinical Significance of the Non-Tuberculous Mycobacteria and the Optimum Methods for Their Isolation [EN] Product Literature

Veterinary Microbiology Capabilities Product Literature

Brochure: para-JEM Brochure Product Literature

Brochure: Mycobacteria Testing Workflow Solutions Product Literature

Can I add antibiotics to OptiPRO SFM? Product FAQ

Answer

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)

Answer Id:: E20494

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Bombyxin is a growth factor for wing imaginal disks in Lepidoptera. Citations & References

  • Authors: Nijhout H Frederik; Grunert Laura W;
  • Journal: Proc Natl Acad Sci U S A
  • PubMed ID: 12429853
Catalog #

Is CTS AIM-V Medium, without phenol red, without antibiotics ready-to-use? Product FAQ

Answer

Yes, CTS AIM-V Medium, without phenol red, without antibiotics is ready-to-use. Additional supplementation with cytokines and growth factors may be required for certain types of cells. Human serum or CTS Immune Cell SR (Cat. Nos. A2596101 and A2596102) can be added to AIM-V medium to promote cell expansion.

Answer Id:: E16474

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VersaTREK REDOX and Myco Media Specifications Product Literature

Brochure: VersaTREK Blood Culture Collection Product Overview Product Literature

Brochure: Blood Culture Solutions Product Literature

VersaTREK Automated Microbial Detection System Product Literature

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