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There are several common problems encountered when culturing cells. Problems in primary cultures may have different causes than the same problem in established cell lines. The table below addresses some of the common problems encountered when culturing cells, along with their possible causes and...

Collect whole blood by venipuncture in EDTA-treated collection tubes. Pipette 1mL EDTA-treated whole blood into a tube containing 10-20 mL of Gibco™ ACK Lysing Buffer at room temperature. Allow the blood sample plus ACK Lysing Buffer to incubate at room temperature for 3 – 5 minutes.

The following is a general procedure to rapidly remove various cell lines from the substratum while maintaining cellular integrity. This procedure is not meant to be universally applicable for all cell lines.

The following is a general procedure to rapidly remove various cell lines from the substratum while maintaining cellular integrity. This procedure is not meant to be universally applicable for all cell lines.

Pipette Tips Pipettes Tubes Vials Microplates Dishes Flasks Cylinders Burettes Beakers and Lids Bottles, Jars and Jugs Carboys Tanks Stoppers and Closures Clamps and Supports Funnels Filters and Filtration Racks Boxes Specialty Lab Glassware Cuvets and Cells Microscope Slides Cell Culture Utensils...

The thawing procedure is stressful to a frozen culture. Using good technique and working quickly ensures that a high proportion of the cells survive the procedure. As with other cell culture procedures, we recommend that you closely follow the instructions provided with your cells and other reagents...

Introduction Materials Ordering Information Protocol for Passaging Adherent Cells Notes on Subculturing Adherent Insect Cells, Cell Culture Plastics Cell Culture Media Fetal Bovine Serum Cell Culture Reagents Cell Culture Antibiotics Media Supplements Growth Factors Mammalian Cell Lines Serum Free...

The following protocols describe general procedures for subculturing mammalian cells in suspension culture . Note that the procedure for passaging insect cells differs from that for mammalian cells on several crucial steps. For more information, refer to Notes on Subculturing Insect Cells .

Antibiotics are compounds which kill or prevent the growth of microorganisms. Antibiotics have different modes of action, typically working by disrupting cell wall synthesis, preventing nucleic acid metabolism, or disturbing a microorganism’s ability to synthesize proteins.

Optimize your culture media with a range of liquid and freeze dried supplements. The range includes supplements that are required to create selectivity in your culture media through the use of antibiotics, nutrients and additives essential for microbial growth, and those that boost microbial growth...

Antibiotics, or antibacterials, are antimicrobial drugs used to treat or prevent bacterial infections. Antibiotics are heralded as the medicinal heroes of the 20th century. However, their effectiveness and accessibility has led to overuse and, in recent years, consequent antibiotic resistance.

Designed to deliver reproducibility,Gibco cell culture reagents are infused with quality, customer-focused innovation, and service excellence from beginning to end. From the most basic formulations to the newest innovations, Gibco products provide superior quality, consistency, and performance—for...

Each Advanced™ medium is the standard published basal formulation, supplemented with NEAA and sodium pyruvate. We’ve also added these ingredients to allow serum reduction: Actual serum concentration reductions will vary with the cell line and basal medium used.

AmnioMAX™ C-100 medium was developed specifically for the in vitro diagnostic testing of human amniotic fluid specimens and chorionic villus sampling. Designed to help minimize diagnostic turnaround time, AmnioMAX™ medium maximizes colony growth while maintaining the cytogenetic analyzability of...

For biotechs seeking to develop novel therapeutics for conditions like Parkinson’s disease, diabetes, and cancer, the development of a relevant cell model for use in preclinical research and development phases is imperative.